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The map label for this gene is rpoA

Identifier: 16125521

GI number: 16125521

Start: 1418278

End: 1419294

Strand: Direct

Name: rpoA

Synonym: CC_1272

Alternate gene names: 16125521

Gene position: 1418278-1419294 (Clockwise)

Preceding gene: 16125520

Following gene: 16125522

Centisome position: 35.31

GC content: 63.32

Gene sequence:

>1017_bases
GTGATCGAAAGAAACTGGAACGAGCTGATCCGTCCTGAGAAGCCGCAAATCGAAACCGGCGCCGATGCGACTCGCAAGGC
CCGTATCGTCGCCGAACCGCTCGAGCGCGGCTTCGGCGTGACGCTCGGCAACGCTCTGCGTCGCGTTCTCCTCTCGTCGC
TGCAAGGCGCCGCCGTCACCGCCATCCAAATCGATGGCGTGGTGCACGAATTCTCCTCGCTCGAAGGCGTCCGTGAAGAC
GTCGTCGACATCGTTCTGAATATCAAGCAACTGGCCGTGCGCATGCACGCCGAAGGTCCGAAGCGCATGACCCTGCGCGC
CACGGGCCCCGGCCCGGTGACCGCTGGTCAGATCGAAACCCCGGCCGACATCGAAATCCTGAACCCCGACCACGTGCTCT
GCACGCTGGACGACGGCGCTTCGGTGCGCATGGAGTTCACGGTCAATAACGGCAAGGGCTACGTCCCGGCCGACCGCAAC
CGTCCGGAAGACGCGCCGATCGGCCTCATCGCCGTCGACGCTCTCTACTCGCCGGTCAAGCGCGTCGCCTACCGCGTCGA
GCCGACCCGTCAGGGTCAGTCGCTGGACTACGACAAGCTGATCCTGGAAGTGGAAACCAACGGCGCCGTCACTCCGGTGG
ACGCCGTGGCCTACGCCGCCCGGATCCTGCAGGACCAACTGCAGATCTTCATCACCTTCGAGGAACCGAAGGCCAAGTCG
GCCGACGAGTCCAAGCCGGAACTGCCGTTCAACCCGGCCCTGCTGAAGAAGGTCGACGAGCTGGAACTGTCGGTCCGTTC
GGCCAACTGCCTGAAGAACGACAACATCGTCTACATCGGCGACCTGATCCAGAAGACCGAAGCCGAGATGCTCCGCACCC
CGAACTTCGGCCGCAAGTCGCTGAACGAGATCAAGGAAGTGCTCGCCGGCATGGGTCTGCACCTCGGCATGGACGTGCCG
AACTGGCCGCCGGAGAACATCGAAGACCTGGCCAAGAAGTTCGAAGACCAGATCTAA

Upstream 100 bases:

>100_bases
CGCCCAAGCGTCGTCGCGTCTAGTACTTTTTAGACCACAATCTTCCTTCGCCGGCTGCGTAAACCGCGGCCGGCGAGCCG
CGTTCAAGGGACCCGACCAC

Downstream 100 bases:

>100_bases
GACTTCCAGCCTAACGCGCTTAATCCGGGCCGCCTGCTCCATTTCTTCGGATTTTGGGGAGGGCGGCCTGGGCCTTTTGA
AGCCGCAAACGCGGTGGAGG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIERNWNELIRPEKPQIETGADATRKARIVAEPLERGFGVTLGNALRRVLLSSLQGAAVTAIQIDGVVHEFSSLEGVRED
VVDIVLNIKQLAVRMHAEGPKRMTLRATGPGPVTAGQIETPADIEILNPDHVLCTLDDGASVRMEFTVNNGKGYVPADRN
RPEDAPIGLIAVDALYSPVKRVAYRVEPTRQGQSLDYDKLILEVETNGAVTPVDAVAYAARILQDQLQIFITFEEPKAKS
ADESKPELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAGMGLHLGMDVP
NWPPENIEDLAKKFEDQI

Sequences:

>Translated_338_residues
MIERNWNELIRPEKPQIETGADATRKARIVAEPLERGFGVTLGNALRRVLLSSLQGAAVTAIQIDGVVHEFSSLEGVRED
VVDIVLNIKQLAVRMHAEGPKRMTLRATGPGPVTAGQIETPADIEILNPDHVLCTLDDGASVRMEFTVNNGKGYVPADRN
RPEDAPIGLIAVDALYSPVKRVAYRVEPTRQGQSLDYDKLILEVETNGAVTPVDAVAYAARILQDQLQIFITFEEPKAKS
ADESKPELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAGMGLHLGMDVP
NWPPENIEDLAKKFEDQI
>Mature_338_residues
MIERNWNELIRPEKPQIETGADATRKARIVAEPLERGFGVTLGNALRRVLLSSLQGAAVTAIQIDGVVHEFSSLEGVRED
VVDIVLNIKQLAVRMHAEGPKRMTLRATGPGPVTAGQIETPADIEILNPDHVLCTLDDGASVRMEFTVNNGKGYVPADRN
RPEDAPIGLIAVDALYSPVKRVAYRVEPTRQGQSLDYDKLILEVETNGAVTPVDAVAYAARILQDQLQIFITFEEPKAKS
ADESKPELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAGMGLHLGMDVP
NWPPENIEDLAKKFEDQI

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=327, Percent_Identity=51.3761467889908, Blast_Score=330, Evalue=9e-92,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CAUCN (B8H4F8)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002516703.1
- ProteinModelPortal:   B8H4F8
- GeneID:   7331785
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01330
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37273; Mature: 37273

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERNWNELIRPEKPQIETGADATRKARIVAEPLERGFGVTLGNALRRVLLSSLQGAAVT
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
AIQIDGVVHEFSSLEGVREDVVDIVLNIKQLAVRMHAEGPKRMTLRATGPGPVTAGQIET
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
PADIEILNPDHVLCTLDDGASVRMEFTVNNGKGYVPADRNRPEDAPIGLIAVDALYSPVK
CCCEEEECCCEEEEEECCCCEEEEEEEEECCCEECCCCCCCCCCCCEEEEEHHHHHHHHH
RVAYRVEPTRQGQSLDYDKLILEVETNGAVTPVDAVAYAARILQDQLQIFITFEEPKAKS
HHHEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCC
ADESKPELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHH
LNEIKEVLAGMGLHLGMDVPNWPPENIEDLAKKFEDQI
HHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIERNWNELIRPEKPQIETGADATRKARIVAEPLERGFGVTLGNALRRVLLSSLQGAAVT
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
AIQIDGVVHEFSSLEGVREDVVDIVLNIKQLAVRMHAEGPKRMTLRATGPGPVTAGQIET
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
PADIEILNPDHVLCTLDDGASVRMEFTVNNGKGYVPADRNRPEDAPIGLIAVDALYSPVK
CCCEEEECCCEEEEEECCCCEEEEEEEEECCCEECCCCCCCCCCCCEEEEEHHHHHHHHH
RVAYRVEPTRQGQSLDYDKLILEVETNGAVTPVDAVAYAARILQDQLQIFITFEEPKAKS
HHHEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCC
ADESKPELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHH
LNEIKEVLAGMGLHLGMDVPNWPPENIEDLAKKFEDQI
HHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA