Click here to switch to the map view.

The map label for this gene is ydgK [H]

Identifier: 16125480

GI number: 16125480

Start: 1388074

End: 1389291

Strand: Direct

Name: ydgK [H]

Synonym: CC_1230

Alternate gene names: 16125480

Gene position: 1388074-1389291 (Clockwise)

Preceding gene: 16125478

Following gene: 16125482

Centisome position: 34.56

GC content: 69.46

Gene sequence:

>1218_bases
ATGACGAACGCCAGCCCGCCGCCCGCCCATGTTCCGTGGCGCCTTGTCCTGCTTCTGGGCGCGCTGACGGCCTTCGCGCC
GATGTCGATCGACATGTATCTGTCGAGCCTTCCCTCGATCGGCCGGACACTGAACGCCGCCCCCGACCAGACCCAGGCGA
CCCTGGCGGCCTTCTTCGCGGGTATGGCCATCGGCCAGGTGTTCTACGGGCCGGCCTCTGACCGGTTCGGCCGTCGGCCG
CCTCTGCTGGTCGGCGTCGGCCTGTTCATCGCCGCCTCGATCGTCTGCGCCGTCGCGCCGAACATCGAGACCCTGATCGC
CGCGCGGTTCGTTCAGGCGCTGGGCGGCTGCGCGGGACCCGTGATCGCCCGCGCGGTGGTGCGTGACCGCTTCAGCCATA
CTGACACGGCGCGCGTCCTTTCGCTGATGACCCTGATCATGGGCCTGGCGCCGGTGCTGGCGCCGCAGCTGGGCGGGGTC
GTCCAGTTTGTGGCGGGCTGGCGCGGGGTGTTCTGGACTCTGGTGATGTTCGGTGTGCTGATCGCCGCCTGGGTCGTGCT
GAGCCTGACCGAATCCCGCTCGGCGGCGACCGAGGCGCAGGCCCGCAGCGAAAATCCGCTGAAGGCCTATGTCGCGCTGT
TCGGGATGCGCCGCCTGATGGGTTACGCCCTGGCGGGGGCCCTGAACGGGGCGGTGCTGTTCACCTACATCTCGGGCGCG
CCGGATCTGGTGATGGGGACCTATGGCCACAGCCCGCTGGTGTTCAATCTGGTCTTCGCCTTAAACGCTGTCGGCATCAT
CGGCGCCAGCCAGATCAATCGTTTCCTGTTGCGCCGCTCAACGCCCGACCAGGTGCTGGCGCGCGCCAGTGTCGCCTCCA
TTGTCGCCGCGCTCCTGCTGACCCTGGCGGCCTGGACGGGCTTCGGCGGGCAATGGACCGTGCTGCCGCTGCTGTTCGCG
GCGCTCTCGCTCTACGGACTGATGGGCGGCAACACCATGGCCGGAGCGCTCAGCGTCGATCCTCAGCGCGCCGGATCAAC
CTCCGCGCTGATGGGCTCGGCCTCGTTCGGGGCCGGGGCCGTGGCGTCCTGGGCGGGGGCTGCGCTGCACGACGGCACGC
CACGTCCGGTGGCCGGCGTGATGTTCGCCTGTCTGGTCGGATCGGCCCTGGCGATCTTCCTGCTGGCCCTGCCGGGCGCG
AAGAAGATTCAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGTTGTTATATGATTGACGGTAAGCTGCTGCGTGCGCGCCAGCCCGGCAAGCGTCATTTCAGGTTTCCGTAGGGGCGA
ACGGGCGTAAAAGGGCGCGC

Downstream 100 bases:

>100_bases
GGCTGGTTGAAACCGCGCCAGGCCATCAGCGCCCAGCCGGCGATGAACGACAGCCCGCCCAACGGCGTAATCGCGCCCAG
GATGCGCGGCGCGCCCAGCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTNASPPPAHVPWRLVLLLGALTAFAPMSIDMYLSSLPSIGRTLNAAPDQTQATLAAFFAGMAIGQVFYGPASDRFGRRP
PLLVGVGLFIAASIVCAVAPNIETLIAARFVQALGGCAGPVIARAVVRDRFSHTDTARVLSLMTLIMGLAPVLAPQLGGV
VQFVAGWRGVFWTLVMFGVLIAAWVVLSLTESRSAATEAQARSENPLKAYVALFGMRRLMGYALAGALNGAVLFTYISGA
PDLVMGTYGHSPLVFNLVFALNAVGIIGASQINRFLLRRSTPDQVLARASVASIVAALLLTLAAWTGFGGQWTVLPLLFA
ALSLYGLMGGNTMAGALSVDPQRAGSTSALMGSASFGAGAVASWAGAALHDGTPRPVAGVMFACLVGSALAIFLLALPGA
KKIQA

Sequences:

>Translated_405_residues
MTNASPPPAHVPWRLVLLLGALTAFAPMSIDMYLSSLPSIGRTLNAAPDQTQATLAAFFAGMAIGQVFYGPASDRFGRRP
PLLVGVGLFIAASIVCAVAPNIETLIAARFVQALGGCAGPVIARAVVRDRFSHTDTARVLSLMTLIMGLAPVLAPQLGGV
VQFVAGWRGVFWTLVMFGVLIAAWVVLSLTESRSAATEAQARSENPLKAYVALFGMRRLMGYALAGALNGAVLFTYISGA
PDLVMGTYGHSPLVFNLVFALNAVGIIGASQINRFLLRRSTPDQVLARASVASIVAALLLTLAAWTGFGGQWTVLPLLFA
ALSLYGLMGGNTMAGALSVDPQRAGSTSALMGSASFGAGAVASWAGAALHDGTPRPVAGVMFACLVGSALAIFLLALPGA
KKIQA
>Mature_404_residues
TNASPPPAHVPWRLVLLLGALTAFAPMSIDMYLSSLPSIGRTLNAAPDQTQATLAAFFAGMAIGQVFYGPASDRFGRRPP
LLVGVGLFIAASIVCAVAPNIETLIAARFVQALGGCAGPVIARAVVRDRFSHTDTARVLSLMTLIMGLAPVLAPQLGGVV
QFVAGWRGVFWTLVMFGVLIAAWVVLSLTESRSAATEAQARSENPLKAYVALFGMRRLMGYALAGALNGAVLFTYISGAP
DLVMGTYGHSPLVFNLVFALNAVGIIGASQINRFLLRRSTPDQVLARASVASIVAALLLTLAAWTGFGGQWTVLPLLFAA
LSLYGLMGGNTMAGALSVDPQRAGSTSALMGSASFGAGAVASWAGAALHDGTPRPVAGVMFACLVGSALAIFLLALPGAK
KIQA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=352, Percent_Identity=30.6818181818182, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790146, Length=389, Percent_Identity=30.8483290488432, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI48994889, Length=359, Percent_Identity=28.4122562674095, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082312, Length=342, Percent_Identity=29.5321637426901, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787065, Length=271, Percent_Identity=26.9372693726937, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790794, Length=260, Percent_Identity=22.6923076923077, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790195, Length=145, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6325414, Length=183, Percent_Identity=23.4972677595628, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41827; Mature: 41696

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
TNASPPPAHVPWRLVLLLGALTAFAPMSIDMYLSSLPSIGRTLNAAPDQTQATLAAFFA
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GMAIGQVFYGPASDRFGRRPPLLVGVGLFIAASIVCAVAPNIETLIAARFVQALGGCAGP
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
VIARAVVRDRFSHTDTARVLSLMTLIMGLAPVLAPQLGGVVQFVAGWRGVFWTLVMFGVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAWVVLSLTESRSAATEAQARSENPLKAYVALFGMRRLMGYALAGALNGAVLFTYISGA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PDLVMGTYGHSPLVFNLVFALNAVGIIGASQINRFLLRRSTPDQVLARASVASIVAALLL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TLAAWTGFGGQWTVLPLLFAALSLYGLMGGNTMAGALSVDPQRAGSTSALMGSASFGAGA
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHCCCCHHHHCCCCCCCCH
VASWAGAALHDGTPRPVAGVMFACLVGSALAIFLLALPGAKKIQA
HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]