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The map label for this gene is glpK [H]

Identifier: 16125473

GI number: 16125473

Start: 1381088

End: 1382578

Strand: Direct

Name: glpK [H]

Synonym: CC_1223

Alternate gene names: 16125473

Gene position: 1381088-1382578 (Clockwise)

Preceding gene: 16125472

Following gene: 16125474

Centisome position: 34.38

GC content: 70.02

Gene sequence:

>1491_bases
ATGGCGCAAGACCTGATCCTGGCCCTGGACCAGGGCACCACCAGCACGCGCGCCATCCTGTTCGATCGGGCGGGGACGGC
CCTGGGCGACGCCTCGCGTCCTCTGCGTCAAAGCTATCCGGCCGACGGCTGGGTCGAGCATGACGCCGAGGAGATCTACG
CCGCCGCCGTCGCTGTGCTGCGCGAGGTCACCGAGGCCTCCGGGCGGATGGCGGACATCGCTGCGATCGGCGTCACCAAC
CAGCGCGAGACCGTGGTCGTCTGGGATCGGGCTACGGGCCGTCCGATCCATCCAGCCATCGTCTGGCAGGATCGCCGCAC
CGCTGACGTCTGCGCGCGCCTCAAGGCCGAGGGGCATGAGCCGAGGGTGACCGAGGTTACGGGCCTGCTGCTCGACCCCT
ATTTCTCGGCGACCAAGATCGCCTGGATCCTCGACAAGACACCCGGCGCCAGGGCCCGGGCGGAGGCCGGCGAGCTTTTG
TGCGGCACCATGGACTGCTGGGTGATCTGGCGACTGACGGGTGGCGCCGTGCACGCGACCGACGCCACCAACGCTTCTCG
AACCTTGCTGTTCGATATCGGCGCTCAGGCCTGGTCGAGCGAGATGCTGGCGTTGTTCAATGTGCCCTCGACGATCCTGC
CCAAGGTGCTGGACTGCGTCGACGACTATGGCGCGACGCAGGCCGACATCCTGGGCCGCGAGGTCCCCATTCGGGGCGTG
GCGGGCGATCAGCAGGCGGCGCTGATGGGGCAGGGCTGTGTCGCTCCGGGCCAGATGAAGGCCACCTACGGCACCGGCTG
CTTCATGCTGATCAATACGGGGACTGACCGCGCCGTCTCGCGGGCGCGCCTGTTGACCACCGTCGCGGCGCGGGTCGGCG
GCAAGACGACCTACGCGCTGGAAGGGTCGATCTTCGTTGCAGGCGCGGCGATCCAGTGGCTGGGCGAGGGGCTCGGAATC
ACGGGCGGTCCCCGCGCCGCTGAGGCGCTGGCCCGGGCGGCCAAGCCGGATCACGCGGTGGTGGTGGTTCCCGCGTTCAC
GGGTCTTGGCGCCCCCTGGTGGGACGCCGAGGCGCGGGGTGGGATCTTCGGTCTGACGCGCGATGCAGGACTACCCGAGA
TCGCCGCCGCCACCTTCGACGCCTGTGCGCTGCAGAGCCGCGACCTGATCGAGGCGATGCGCGCCGATGCGCCCACGGCC
TTTGGCGAAGCGGCGCAACTGCGGATCGACGGCGGTATGGCCAAGAGCGCCTGGTTCTCGCAGCGGCTGGCCGATCTCAC
GGGGCTCTCGGTGCGCCGCGCCAGCTATCAGGAGACCACGGCCCTGGGGGCGGCGTTGTTCGCCGCCGTCGGCGCGGGTC
TCTACGGGTCGATCGAAGAGGCCGCCGAAGCCAGTCCAGCCGCCGAGACCTTAGCGCCGGCCATGGACGACCATGTGCGG
GAAGCCGCCTACGCCCGTTGGCTCGACGCGGTGCCGCGCGTTCGACTTTAG

Upstream 100 bases:

>100_bases
GACCTTGCGGCCCTCAAATGAAAACATGCTTAAACAGGGCAAAGACGCCGATGCGCTGTTACGCGTCGAGATGTCGCAAG
CTTGAGTGGAGGAACTGTCG

Downstream 100 bases:

>100_bases
TTGATCACCCGTTGCGGTGACATCGCTGTCATGGTCTCTAGCCCGCAAACAATCATAAGCGGAGGCCGCCATGACCACTC
GACGCGCCATGCTCGGCGCA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAQDLILALDQGTTSTRAILFDRAGTALGDASRPLRQSYPADGWVEHDAEEIYAAAVAVLREVTEASGRMADIAAIGVTN
QRETVVVWDRATGRPIHPAIVWQDRRTADVCARLKAEGHEPRVTEVTGLLLDPYFSATKIAWILDKTPGARARAEAGELL
CGTMDCWVIWRLTGGAVHATDATNASRTLLFDIGAQAWSSEMLALFNVPSTILPKVLDCVDDYGATQADILGREVPIRGV
AGDQQAALMGQGCVAPGQMKATYGTGCFMLINTGTDRAVSRARLLTTVAARVGGKTTYALEGSIFVAGAAIQWLGEGLGI
TGGPRAAEALARAAKPDHAVVVVPAFTGLGAPWWDAEARGGIFGLTRDAGLPEIAAATFDACALQSRDLIEAMRADAPTA
FGEAAQLRIDGGMAKSAWFSQRLADLTGLSVRRASYQETTALGAALFAAVGAGLYGSIEEAAEASPAAETLAPAMDDHVR
EAAYARWLDAVPRVRL

Sequences:

>Translated_496_residues
MAQDLILALDQGTTSTRAILFDRAGTALGDASRPLRQSYPADGWVEHDAEEIYAAAVAVLREVTEASGRMADIAAIGVTN
QRETVVVWDRATGRPIHPAIVWQDRRTADVCARLKAEGHEPRVTEVTGLLLDPYFSATKIAWILDKTPGARARAEAGELL
CGTMDCWVIWRLTGGAVHATDATNASRTLLFDIGAQAWSSEMLALFNVPSTILPKVLDCVDDYGATQADILGREVPIRGV
AGDQQAALMGQGCVAPGQMKATYGTGCFMLINTGTDRAVSRARLLTTVAARVGGKTTYALEGSIFVAGAAIQWLGEGLGI
TGGPRAAEALARAAKPDHAVVVVPAFTGLGAPWWDAEARGGIFGLTRDAGLPEIAAATFDACALQSRDLIEAMRADAPTA
FGEAAQLRIDGGMAKSAWFSQRLADLTGLSVRRASYQETTALGAALFAAVGAGLYGSIEEAAEASPAAETLAPAMDDHVR
EAAYARWLDAVPRVRL
>Mature_495_residues
AQDLILALDQGTTSTRAILFDRAGTALGDASRPLRQSYPADGWVEHDAEEIYAAAVAVLREVTEASGRMADIAAIGVTNQ
RETVVVWDRATGRPIHPAIVWQDRRTADVCARLKAEGHEPRVTEVTGLLLDPYFSATKIAWILDKTPGARARAEAGELLC
GTMDCWVIWRLTGGAVHATDATNASRTLLFDIGAQAWSSEMLALFNVPSTILPKVLDCVDDYGATQADILGREVPIRGVA
GDQQAALMGQGCVAPGQMKATYGTGCFMLINTGTDRAVSRARLLTTVAARVGGKTTYALEGSIFVAGAAIQWLGEGLGIT
GGPRAAEALARAAKPDHAVVVVPAFTGLGAPWWDAEARGGIFGLTRDAGLPEIAAATFDACALQSRDLIEAMRADAPTAF
GEAAQLRIDGGMAKSAWFSQRLADLTGLSVRRASYQETTALGAALFAAVGAGLYGSIEEAAEASPAAETLAPAMDDHVRE
AAYARWLDAVPRVRL

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=39.4422310756972, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=39.4422310756972, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=38.6587771203156, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=40.4382470119522, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI88196792, Length=506, Percent_Identity=27.8656126482213, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=46.4646464646465, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1789987, Length=491, Percent_Identity=24.847250509165, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1790005, Length=491, Percent_Identity=23.6252545824847, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789168, Length=478, Percent_Identity=24.2677824267782, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787789, Length=490, Percent_Identity=26.3265306122449, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=499, Percent_Identity=37.2745490981964, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=21.8146718146718, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71986147, Length=265, Percent_Identity=25.6603773584906, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321755, Length=550, Percent_Identity=31.4545454545455, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI17864214, Length=502, Percent_Identity=38.0478087649402, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24654573, Length=443, Percent_Identity=39.2776523702032, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24655555, Length=469, Percent_Identity=37.7398720682303, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655560, Length=469, Percent_Identity=37.7398720682303, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655570, Length=469, Percent_Identity=37.7398720682303, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655574, Length=469, Percent_Identity=37.7398720682303, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655565, Length=469, Percent_Identity=37.7398720682303, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=30.9297912713473, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=31.3765182186235, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=26.6260162601626, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21357867, Length=514, Percent_Identity=25.875486381323, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24656372, Length=328, Percent_Identity=27.4390243902439, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45552909, Length=247, Percent_Identity=28.3400809716599, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 52261; Mature: 52130

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDLILALDQGTTSTRAILFDRAGTALGDASRPLRQSYPADGWVEHDAEEIYAAAVAVL
CCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHH
REVTEASGRMADIAAIGVTNQRETVVVWDRATGRPIHPAIVWQDRRTADVCARLKAEGHE
HHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCCCC
PRVTEVTGLLLDPYFSATKIAWILDKTPGARARAEAGELLCGTMDCWVIWRLTGGAVHAT
CCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHCCCCEEECCCCEEEEEEECCCEEEEC
DATNASRTLLFDIGAQAWSSEMLALFNVPSTILPKVLDCVDDYGATQADILGREVPIRGV
CCCCCCCEEEEECCCHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
AGDQQAALMGQGCVAPGQMKATYGTGCFMLINTGTDRAVSRARLLTTVAARVGGKTTYAL
CCCCHHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEE
EGSIFVAGAAIQWLGEGLGITGGPRAAEALARAAKPDHAVVVVPAFTGLGAPWWDAEARG
CCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCC
GIFGLTRDAGLPEIAAATFDACALQSRDLIEAMRADAPTAFGEAAQLRIDGGMAKSAWFS
CEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
QRLADLTGLSVRRASYQETTALGAALFAAVGAGLYGSIEEAAEASPAAETLAPAMDDHVR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
EAAYARWLDAVPRVRL
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQDLILALDQGTTSTRAILFDRAGTALGDASRPLRQSYPADGWVEHDAEEIYAAAVAVL
CCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHH
REVTEASGRMADIAAIGVTNQRETVVVWDRATGRPIHPAIVWQDRRTADVCARLKAEGHE
HHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCCCC
PRVTEVTGLLLDPYFSATKIAWILDKTPGARARAEAGELLCGTMDCWVIWRLTGGAVHAT
CCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHCCCCEEECCCCEEEEEEECCCEEEEC
DATNASRTLLFDIGAQAWSSEMLALFNVPSTILPKVLDCVDDYGATQADILGREVPIRGV
CCCCCCCEEEEECCCHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
AGDQQAALMGQGCVAPGQMKATYGTGCFMLINTGTDRAVSRARLLTTVAARVGGKTTYAL
CCCCHHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEE
EGSIFVAGAAIQWLGEGLGITGGPRAAEALARAAKPDHAVVVVPAFTGLGAPWWDAEARG
CCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCC
GIFGLTRDAGLPEIAAATFDACALQSRDLIEAMRADAPTAFGEAAQLRIDGGMAKSAWFS
CEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
QRLADLTGLSVRRASYQETTALGAALFAAVGAGLYGSIEEAAEASPAAETLAPAMDDHVR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
EAAYARWLDAVPRVRL
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]