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The map label for this gene is rfaF [H]

Identifier: 16124641

GI number: 16124641

Start: 405999

End: 407213

Strand: Direct

Name: rfaF [H]

Synonym: CC_0386

Alternate gene names: 16124641

Gene position: 405999-407213 (Clockwise)

Preceding gene: 16124638

Following gene: 16124642

Centisome position: 10.11

GC content: 68.72

Gene sequence:

>1215_bases
ATGACACAGAGGGCCTTTCCGATCCTTTTCATCACGGCGACGCGTATCGGTGACGCGGTGCTGTCGTCCGGCCTGATCAA
GATGCTGGCCGACCAGATCCCCAACGCGCGCTTCACCATCGTCGCCGGCCCGCTGGCCGCGCCGCTCTTCGCTCACGTCC
CCGGGCTCGACCGGGTGATCGTGATGGAGAAGGGCAAGGGCAAGGGCCACTGGTTCAAGCTCTGGCGCCAGGTGCGCCAC
AAGAAGTGGAGCCTGATCGTCGACCTGCGCGGTTCGGCCACGGCGCTCTTCCTGCGCCGCGACAAGCGCGCCATCTGGAA
GAAGGTCCCGGGCGAGGTCGTCCACAAGGTGGTGGACGCGGCCCGCACGCTGAAGCTCGAGGGCGATCCGCCGCCGCCCT
ATCTCTACATCACGCCCGAGGTGCAGGCCCTGGCGGACGAACTGCTGGGCCTGACCGAGGGTGGCGAGACCGGACCGATC
CTGGCCGTGGGCCCGGCGGCCAACTGGATCGGCAAGGTCTGGCCGATCGAGCGCTTCACCCAGACCGCCGCCCAGCTGCT
GGACAAGGACGGCCCGATGGCCGGCGGCCGGCTGCTGATCCTGGGCGGTCCGGAAGACACCCGCATGGTCGAGGAACTGC
GCATGGCCTCGGCGCGCGGGCGCACCATCGACCTGACCGGCAAGGTGGATCTGCTCACCGCCTATGCCTGCCTGAAGCGC
GCCAGCCTGTTCATCGGCAACGACTCGGGCCTGATGCACATCGCCGCCGCCGCCGGCACGCCGACGGTCGGGCTGTTCGG
TCCCTCCGACGAGCGCCGCTATGCGCCGTGGGGCGAGCACGCTGTGGCGGTGCGCGGGCCAAGGTCGTTCGATCAGTTCC
TGACCATCGATCCCGAGCTCTCCCAGGCGATCCGCCACATGAGCGATCTTCCGGTGACCACGGTGTTCAAGGCCGCCAAG
GATCTGCTGGCCAAGGTGTCTGGGACCGTCCCGTCGATTGTGTCCGATGCCGTTTCGGAAGCCGAAATCGTCGGATCGAC
GCCCCCAGCCGAGCCTGAGGCCATGGCCCAGACCACCGTCGATCCGATCGAGGCGGCGCCCACCGACGCGCTCAGCGACC
CTGCCGATGAGGGCGAGGCAAAGCGCCCGATCGAGGACGCGGTTGAGCCGCTCAAGACCCAACGCCGCGCAGCGCGGCCG
AGGAAAGCCCGATGA

Upstream 100 bases:

>100_bases
GGCACATGTGGCGCCGTGTCGCGGGCGACGCCAGTCCTTGAGGTAACTCAAGTGTTAACGTGGGGTGCGCCGACTCACCG
ACCGGCCTATAGGTGTACCG

Downstream 100 bases:

>100_bases
AGGCCGTCGTCGCTCTTTGTGCGCTGATCGTCCTGGCGCCGGGCGCAGCCTTCGCCGGGGCGCCGGGTGAGCAGTTCTTC
GAGCGCCTGAGCGCGCTGTG

Product: ADP-heptose--LPS-heptosyl transferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTQRAFPILFITATRIGDAVLSSGLIKMLADQIPNARFTIVAGPLAAPLFAHVPGLDRVIVMEKGKGKGHWFKLWRQVRH
KKWSLIVDLRGSATALFLRRDKRAIWKKVPGEVVHKVVDAARTLKLEGDPPPPYLYITPEVQALADELLGLTEGGETGPI
LAVGPAANWIGKVWPIERFTQTAAQLLDKDGPMAGGRLLILGGPEDTRMVEELRMASARGRTIDLTGKVDLLTAYACLKR
ASLFIGNDSGLMHIAAAAGTPTVGLFGPSDERRYAPWGEHAVAVRGPRSFDQFLTIDPELSQAIRHMSDLPVTTVFKAAK
DLLAKVSGTVPSIVSDAVSEAEIVGSTPPAEPEAMAQTTVDPIEAAPTDALSDPADEGEAKRPIEDAVEPLKTQRRAARP
RKAR

Sequences:

>Translated_404_residues
MTQRAFPILFITATRIGDAVLSSGLIKMLADQIPNARFTIVAGPLAAPLFAHVPGLDRVIVMEKGKGKGHWFKLWRQVRH
KKWSLIVDLRGSATALFLRRDKRAIWKKVPGEVVHKVVDAARTLKLEGDPPPPYLYITPEVQALADELLGLTEGGETGPI
LAVGPAANWIGKVWPIERFTQTAAQLLDKDGPMAGGRLLILGGPEDTRMVEELRMASARGRTIDLTGKVDLLTAYACLKR
ASLFIGNDSGLMHIAAAAGTPTVGLFGPSDERRYAPWGEHAVAVRGPRSFDQFLTIDPELSQAIRHMSDLPVTTVFKAAK
DLLAKVSGTVPSIVSDAVSEAEIVGSTPPAEPEAMAQTTVDPIEAAPTDALSDPADEGEAKRPIEDAVEPLKTQRRAARP
RKAR
>Mature_403_residues
TQRAFPILFITATRIGDAVLSSGLIKMLADQIPNARFTIVAGPLAAPLFAHVPGLDRVIVMEKGKGKGHWFKLWRQVRHK
KWSLIVDLRGSATALFLRRDKRAIWKKVPGEVVHKVVDAARTLKLEGDPPPPYLYITPEVQALADELLGLTEGGETGPIL
AVGPAANWIGKVWPIERFTQTAAQLLDKDGPMAGGRLLILGGPEDTRMVEELRMASARGRTIDLTGKVDLLTAYACLKRA
SLFIGNDSGLMHIAAAAGTPTVGLFGPSDERRYAPWGEHAVAVRGPRSFDQFLTIDPELSQAIRHMSDLPVTTVFKAAKD
LLAKVSGTVPSIVSDAVSEAEIVGSTPPAEPEAMAQTTVDPIEAAPTDALSDPADEGEAKRPIEDAVEPLKTQRRAARPR
KAR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=325, Percent_Identity=24.6153846153846, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790062, Length=336, Percent_Identity=23.5119047619048, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 43604; Mature: 43473

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRAFPILFITATRIGDAVLSSGLIKMLADQIPNARFTIVAGPLAAPLFAHVPGLDRVI
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEE
VMEKGKGKGHWFKLWRQVRHKKWSLIVDLRGSATALFLRRDKRAIWKKVPGEVVHKVVDA
EEECCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEHHHHHHHHHCCHHHHHHHHHH
ARTLKLEGDPPPPYLYITPEVQALADELLGLTEGGETGPILAVGPAANWIGKVWPIERFT
HHEEEECCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHCCCCHHHHH
QTAAQLLDKDGPMAGGRLLILGGPEDTRMVEELRMASARGRTIDLTGKVDLLTAYACLKR
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
ASLFIGNDSGLMHIAAAAGTPTVGLFGPSDERRYAPWGEHAVAVRGPRSFDQFLTIDPEL
CEEEEECCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHEEECHHH
SQAIRHMSDLPVTTVFKAAKDLLAKVSGTVPSIVSDAVSEAEIVGSTPPAEPEAMAQTTV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
DPIEAAPTDALSDPADEGEAKRPIEDAVEPLKTQRRAARPRKAR
CCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQRAFPILFITATRIGDAVLSSGLIKMLADQIPNARFTIVAGPLAAPLFAHVPGLDRVI
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEE
VMEKGKGKGHWFKLWRQVRHKKWSLIVDLRGSATALFLRRDKRAIWKKVPGEVVHKVVDA
EEECCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEHHHHHHHHHCCHHHHHHHHHH
ARTLKLEGDPPPPYLYITPEVQALADELLGLTEGGETGPILAVGPAANWIGKVWPIERFT
HHEEEECCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHCCCCHHHHH
QTAAQLLDKDGPMAGGRLLILGGPEDTRMVEELRMASARGRTIDLTGKVDLLTAYACLKR
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
ASLFIGNDSGLMHIAAAAGTPTVGLFGPSDERRYAPWGEHAVAVRGPRSFDQFLTIDPEL
CEEEEECCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHEEECHHH
SQAIRHMSDLPVTTVFKAAKDLLAKVSGTVPSIVSDAVSEAEIVGSTPPAEPEAMAQTTV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
DPIEAAPTDALSDPADEGEAKRPIEDAVEPLKTQRRAARPRKAR
CCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]