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The map label for this gene is iaaA [H]

Identifier: 16124352

GI number: 16124352

Start: 107402

End: 108280

Strand: Direct

Name: iaaA [H]

Synonym: CC_0097

Alternate gene names: 16124352

Gene position: 107402-108280 (Clockwise)

Preceding gene: 16124345

Following gene: 16124353

Centisome position: 2.67

GC content: 73.49

Gene sequence:

>879_bases
ATGTCAAACAAACGTTTTAGTCTCGCCCTGCACGGCGGCGCCGGAGCCAAGCGCGGCCACGACTACGGCGTCGAGATCGC
CCATATGCGCGGCCTCGTCGAAGCCGCCCGCGATCGCCTGGCCGCCGGCGCCGGCGCGCTCGACGTGGCGGTCGAGACCG
TGGTCGGCCTTGAGGCCAGCGGGCTCTACATCGCCGGCAAGGGCGCCTCGCCCAACGCCGACGGCGAATATGAGCTGGAC
GCCAGCCTGATGGACGGCGCCACCCTGCGCGCCGGGTCGGTCGCCGCCCTGCAGGGCTTCAAGAGCCCGATCCTGGCCGC
CCGGGCGGTGATGGAGCACACCCCGCACGTGATGCTGGCCGGCCAGGGCGCTATCGCCTTCGCCCGCGAGCAGGGCCTGG
AGACCGTCGAGGATCCCGACGCCTGGTTCACCCGCGCCGGCGCCTTCGAGGACAACCATCCGCCGGACGCGCTGCCCACC
GGCACCGTCGGCTGCGTGGTCCGCGACGGCGAAGGGCGCCTCGCCGCCGCCACCTCCACCGCCGGAGTGTTCGGCAAGCG
GCCCGGCCGGGTGGGCGACAGCCCCATCATCGGCGCCGGGGCCTGGGCCGACGGCCACGCCGCCGTCTCTTGCACCGGCC
AGGGCGAGTACTTCATCCGCGCCGCCGTCGCCGCCCAGATCGCCCACCGCGTCCGCTTCGGCGGCGAGGCCCTGGACGCG
GCGGCCCAGGCTGCGATCGACAGCGTCGCGGCGCTGGGCGGTCACGGCGGACTGATCGCGGTCGATCGTGACGGCAACAT
CGCCATGCCCTTCGTCTCCAGCGGTCTGAAACGCGCCGCCCTGATGCCGGACGGAACCATCGTCAGCGCGGCGTTCTAG

Upstream 100 bases:

>100_bases
CGCCGACTTCCTTGCTGAGCACCGCCTTCATATCGCCTCTCCCACGTTTCTTGGCTAAGAGGCCGGACGCTAGCGCGGGA
CGTCGAGCCAAGGAAGCAGA

Downstream 100 bases:

>100_bases
AAATCCTCCCCCAGCGGGGGAGGCGGCGCGAAGCGCCGGAGGAGGAAGTTCGGTTGAGCCGGCCTCTTCCCCCTCCGTCG
GCTGCGCCGACACCTCCCCC

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSNKRFSLALHGGAGAKRGHDYGVEIAHMRGLVEAARDRLAAGAGALDVAVETVVGLEASGLYIAGKGASPNADGEYELD
ASLMDGATLRAGSVAALQGFKSPILAARAVMEHTPHVMLAGQGAIAFAREQGLETVEDPDAWFTRAGAFEDNHPPDALPT
GTVGCVVRDGEGRLAAATSTAGVFGKRPGRVGDSPIIGAGAWADGHAAVSCTGQGEYFIRAAVAAQIAHRVRFGGEALDA
AAQAAIDSVAALGGHGGLIAVDRDGNIAMPFVSSGLKRAALMPDGTIVSAAF

Sequences:

>Translated_292_residues
MSNKRFSLALHGGAGAKRGHDYGVEIAHMRGLVEAARDRLAAGAGALDVAVETVVGLEASGLYIAGKGASPNADGEYELD
ASLMDGATLRAGSVAALQGFKSPILAARAVMEHTPHVMLAGQGAIAFAREQGLETVEDPDAWFTRAGAFEDNHPPDALPT
GTVGCVVRDGEGRLAAATSTAGVFGKRPGRVGDSPIIGAGAWADGHAAVSCTGQGEYFIRAAVAAQIAHRVRFGGEALDA
AAQAAIDSVAALGGHGGLIAVDRDGNIAMPFVSSGLKRAALMPDGTIVSAAF
>Mature_291_residues
SNKRFSLALHGGAGAKRGHDYGVEIAHMRGLVEAARDRLAAGAGALDVAVETVVGLEASGLYIAGKGASPNADGEYELDA
SLMDGATLRAGSVAALQGFKSPILAARAVMEHTPHVMLAGQGAIAFAREQGLETVEDPDAWFTRAGAFEDNHPPDALPTG
TVGCVVRDGEGRLAAATSTAGVFGKRPGRVGDSPIIGAGAWADGHAAVSCTGQGEYFIRAAVAAQIAHRVRFGGEALDAA
AQAAIDSVAALGGHGGLIAVDRDGNIAMPFVSSGLKRAALMPDGTIVSAAF

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=303, Percent_Identity=35.6435643564356, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI145275200, Length=303, Percent_Identity=35.6435643564356, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI98991769, Length=310, Percent_Identity=30.9677419354839, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI285002251, Length=219, Percent_Identity=33.7899543378995, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI285002253, Length=219, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787050, Length=294, Percent_Identity=44.8979591836735, Blast_Score=207, Evalue=1e-54,
Organism=Drosophila melanogaster, GI18921183, Length=315, Percent_Identity=33.968253968254, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221330102, Length=247, Percent_Identity=35.6275303643725, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24652135, Length=247, Percent_Identity=35.6275303643725, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=32.089552238806, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 29463; Mature: 29332

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKRFSLALHGGAGAKRGHDYGVEIAHMRGLVEAARDRLAAGAGALDVAVETVVGLEAS
CCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
GLYIAGKGASPNADGEYELDASLMDGATLRAGSVAALQGFKSPILAARAVMEHTPHVMLA
CEEEEECCCCCCCCCCEEECCHHHCCCEECCCCHHHHHHCCHHHHHHHHHHHCCCEEEEE
GQGAIAFAREQGLETVEDPDAWFTRAGAFEDNHPPDALPTGTVGCVVRDGEGRLAAATST
CCCCEEEHHHCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECC
AGVFGKRPGRVGDSPIIGAGAWADGHAAVSCTGQGEYFIRAAVAAQIAHRVRFGGEALDA
CCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHH
AAQAAIDSVAALGGHGGLIAVDRDGNIAMPFVSSGLKRAALMPDGTIVSAAF
HHHHHHHHHHHHCCCCCEEEEECCCCEECHHHHCCCHHEEECCCCCEEEECC
>Mature Secondary Structure 
SNKRFSLALHGGAGAKRGHDYGVEIAHMRGLVEAARDRLAAGAGALDVAVETVVGLEAS
CCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
GLYIAGKGASPNADGEYELDASLMDGATLRAGSVAALQGFKSPILAARAVMEHTPHVMLA
CEEEEECCCCCCCCCCEEECCHHHCCCEECCCCHHHHHHCCHHHHHHHHHHHCCCEEEEE
GQGAIAFAREQGLETVEDPDAWFTRAGAFEDNHPPDALPTGTVGCVVRDGEGRLAAATST
CCCCEEEHHHCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECC
AGVFGKRPGRVGDSPIIGAGAWADGHAAVSCTGQGEYFIRAAVAAQIAHRVRFGGEALDA
CCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHH
AAQAAIDSVAALGGHGGLIAVDRDGNIAMPFVSSGLKRAALMPDGTIVSAAF
HHHHHHHHHHHHCCCCCEEEEECCCCEECHHHHCCCHHEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]