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The map label for this gene is rhtB [H]

Identifier: 16124285

GI number: 16124285

Start: 32914

End: 33546

Strand: Direct

Name: rhtB [H]

Synonym: CC_0029

Alternate gene names: 16124285

Gene position: 32914-33546 (Clockwise)

Preceding gene: 16124280

Following gene: 16124286

Centisome position: 0.82

GC content: 68.4

Gene sequence:

>633_bases
GTGCCCGAGGCCAAGACCTGGATCGCCTTCACCCTTGTCTGTCTGAGCATGGCCCTGACGCCTGGGCCGAACATGCTCTA
TCTGGTCAGCCGCTCGATCTGTCAGGGCCGGTGGGCCGGGATCGTGTCGCTGATCGGCACGGCGGCGGGTTTCGTCGTCT
ACCTGCTGTGCGCGGCGCTGGGCATCACCGCCCTCCTGATGGCCGCGCCGATCGCCTACGACCTGCTCCGTTTCGGCGGC
GCGTTGTATCTCGCCTGGCTGGCGTGGCAGGCGATCAGACCCGGCGGCGCCTCGCCGTTCCAGGTGCGCGAGCTGCCCAA
GGACAGCCCCGCCAAGCTGATCACGATGGGCTTCGTGACCAACATGCTGAACCCAAAGGCGGCGATGTTGTACCTGTCGC
TGCTGCCGCAGTTCATCAAGCCCGAGCATGGGGGCGTCTTCACCCAGTCCCTGGCGCTGGGCCTGACCCAGATCTGCGTC
AGCCTGACCGTGAACGGCGTCATCTGCCTGGCGGCCGGAACCATCGCCGGCTTCCTGGCCGCCCGCCCCGGTTTCGCGCT
TGTCCAGCGCTGGCTGATGGCCACGGTGCTCGGCGGACTGGCGGTGCGGATGGCCACCGAGGCGCGGCGCTGA

Upstream 100 bases:

>100_bases
GCGCGACTGGCGTTCGGAGGGAAATGACCCTCGGGAAGCGTGGACTTTCAGAGCCGTTCGCCTGGGCGCCTAACTCCAAT
CTGCCAAAGGAGTCGCGCCT

Downstream 100 bases:

>100_bases
TCGCGGGCTTGACGCCCTGGCGAGCGCGCGGGCCTATCGGTCGGGTTCTTGGAGTCGCCGATGAAGACCGTCCCCGCCGC
CATCCTGGCCGCCGCCCTGG

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPEAKTWIAFTLVCLSMALTPGPNMLYLVSRSICQGRWAGIVSLIGTAAGFVVYLLCAALGITALLMAAPIAYDLLRFGG
ALYLAWLAWQAIRPGGASPFQVRELPKDSPAKLITMGFVTNMLNPKAAMLYLSLLPQFIKPEHGGVFTQSLALGLTQICV
SLTVNGVICLAAGTIAGFLAARPGFALVQRWLMATVLGGLAVRMATEARR

Sequences:

>Translated_210_residues
MPEAKTWIAFTLVCLSMALTPGPNMLYLVSRSICQGRWAGIVSLIGTAAGFVVYLLCAALGITALLMAAPIAYDLLRFGG
ALYLAWLAWQAIRPGGASPFQVRELPKDSPAKLITMGFVTNMLNPKAAMLYLSLLPQFIKPEHGGVFTQSLALGLTQICV
SLTVNGVICLAAGTIAGFLAARPGFALVQRWLMATVLGGLAVRMATEARR
>Mature_209_residues
PEAKTWIAFTLVCLSMALTPGPNMLYLVSRSICQGRWAGIVSLIGTAAGFVVYLLCAALGITALLMAAPIAYDLLRFGGA
LYLAWLAWQAIRPGGASPFQVRELPKDSPAKLITMGFVTNMLNPKAAMLYLSLLPQFIKPEHGGVFTQSLALGLTQICVS
LTVNGVICLAAGTIAGFLAARPGFALVQRWLMATVLGGLAVRMATEARR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=159, Percent_Identity=28.9308176100629, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788099, Length=197, Percent_Identity=26.3959390862944, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22314; Mature: 22183

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAKTWIAFTLVCLSMALTPGPNMLYLVSRSICQGRWAGIVSLIGTAAGFVVYLLCAAL
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GITALLMAAPIAYDLLRFGGALYLAWLAWQAIRPGGASPFQVRELPKDSPAKLITMGFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHH
NMLNPKAAMLYLSLLPQFIKPEHGGVFTQSLALGLTQICVSLTVNGVICLAAGTIAGFLA
HHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARPGFALVQRWLMATVLGGLAVRMATEARR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEAKTWIAFTLVCLSMALTPGPNMLYLVSRSICQGRWAGIVSLIGTAAGFVVYLLCAAL
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GITALLMAAPIAYDLLRFGGALYLAWLAWQAIRPGGASPFQVRELPKDSPAKLITMGFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHH
NMLNPKAAMLYLSLLPQFIKPEHGGVFTQSLALGLTQICVSLTVNGVICLAAGTIAGFLA
HHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARPGFALVQRWLMATVLGGLAVRMATEARR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]