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The map label for this gene is tfbC

Identifier: 16120252

GI number: 16120252

Start: 282126

End: 283079

Strand: Reverse

Name: tfbC

Synonym: VNG6351G

Alternate gene names: 16120252

Gene position: 283079-282126 (Counterclockwise)

Preceding gene: 16120253

Following gene: 16120251

Centisome position: 77.47

GC content: 59.43

Gene sequence:

>954_bases
ATGGAACGGGCGACTCGGGAACGTGAAAAGGAACAGCGTGAGCAAGCACAAACGAACGACGAGGCACAGCAGTGCCCGGA
GTGCAACTCAGCCAACGTGATCACCGATCAGAGCGAACGAGTCTGTGAGGACTGCGGGCTCGTTCTTGAGGACGACCAAA
TTGATCACGGCCCGGAGTGGCGGGCGTTCAACTCCTCAGAGCGCGACCAGAAATCACGCGTGGGCGCGCCGACGACGAAA
ACGATGCACGATAAGGGGCTGACGACCCAGATAGACTGGAAGGACAAGGATGCCTACGGTCGCTCACTTGACGCGAAGAA
ACGAAACCAGATGAATCGCCTCCGGAAATGGCAGGAGCGCATCCGGACGAAGGACGCGGGTGAACGCAACCTCCAGTTCG
CGCTCTCTGAGATCGATCGGATGGCATCCGCGCTTGGCGTCCCACGTAGTGTTCGCGAGGTCGCATCGGTCATCTATCGG
CGCGCGCTCAAAGAGGACCTCATCCGTGGGCGCTCAATCGAGGGCGTCGCGACTGCCTGCCTCTACGCCGCCTGCCGCCA
AGAGGGCATCCCGCGAACCCTCGAAGAAGTGACGGAAGTCGCCCGCATCGACCAGAAGGAGATCGGACGGACGTATCGCT
ATGTCGCCCAAGAACTCTCCCTCGAAATTCAGCCAACCGACCCCAAGGAATACCTCCCCCGCTTCGCGAGCGACCTCGAA
CTTTCTGAAGAAGTCATCGCAAAGGCCCGCGAAATCATCGACACCTCCGCCGAACAAGGACTACTCTCTGGGAAATCACC
ATCCGGATTTGCCGCCGCCGCCATCTACGCGGCAAGCCTCCTCTGCAACGAGAAGAAAACCCAGCGCGAAGTCGCCAACG
TCGCAAACGTCACCGAAGTCACCATCCGCAACCGCTACCAAGAACAAATCGAAGCGATGGGCTTCGGAGTATAA

Upstream 100 bases:

>100_bases
TTGGCCTTCGGGCAGAACTGGAGTCTGCACTGCAATCTGTCTCTTTGGCTGGCGACAAGCAAAGGCATATATATATACTT
ACTAGGTTGTCATATGGTGA

Downstream 100 bases:

>100_bases
ACTGCTAACTCGGCTCCTTTACTGGACTCGCCAGCCCAACACACTGACCGAGGATCGCTTATTGACGAAAGCGTGACGCC
CGCTCTGTTGCGATGTTCAG

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 3

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MERATREREKEQREQAQTNDEAQQCPECNSANVITDQSERVCEDCGLVLEDDQIDHGPEWRAFNSSERDQKSRVGAPTTK
TMHDKGLTTQIDWKDKDAYGRSLDAKKRNQMNRLRKWQERIRTKDAGERNLQFALSEIDRMASALGVPRSVREVASVIYR
RALKEDLIRGRSIEGVATACLYAACRQEGIPRTLEEVTEVARIDQKEIGRTYRYVAQELSLEIQPTDPKEYLPRFASDLE
LSEEVIAKAREIIDTSAEQGLLSGKSPSGFAAAAIYAASLLCNEKKTQREVANVANVTEVTIRNRYQEQIEAMGFGV

Sequences:

>Translated_317_residues
MERATREREKEQREQAQTNDEAQQCPECNSANVITDQSERVCEDCGLVLEDDQIDHGPEWRAFNSSERDQKSRVGAPTTK
TMHDKGLTTQIDWKDKDAYGRSLDAKKRNQMNRLRKWQERIRTKDAGERNLQFALSEIDRMASALGVPRSVREVASVIYR
RALKEDLIRGRSIEGVATACLYAACRQEGIPRTLEEVTEVARIDQKEIGRTYRYVAQELSLEIQPTDPKEYLPRFASDLE
LSEEVIAKAREIIDTSAEQGLLSGKSPSGFAAAAIYAASLLCNEKKTQREVANVANVTEVTIRNRYQEQIEAMGFGV
>Mature_317_residues
MERATREREKEQREQAQTNDEAQQCPECNSANVITDQSERVCEDCGLVLEDDQIDHGPEWRAFNSSERDQKSRVGAPTTK
TMHDKGLTTQIDWKDKDAYGRSLDAKKRNQMNRLRKWQERIRTKDAGERNLQFALSEIDRMASALGVPRSVREVASVIYR
RALKEDLIRGRSIEGVATACLYAACRQEGIPRTLEEVTEVARIDQKEIGRTYRYVAQELSLEIQPTDPKEYLPRFASDLE
LSEEVIAKAREIIDTSAEQGLLSGKSPSGFAAAAIYAASLLCNEKKTQREVANVANVTEVTIRNRYQEQIEAMGFGV

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=286, Percent_Identity=34.965034965035, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI22035556, Length=188, Percent_Identity=30.3191489361702, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17564892, Length=292, Percent_Identity=33.9041095890411, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531397, Length=174, Percent_Identity=27.0114942528736, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325343, Length=301, Percent_Identity=28.2392026578073, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321685, Length=172, Percent_Identity=27.3255813953488, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136758, Length=270, Percent_Identity=34.4444444444444, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI281361961, Length=299, Percent_Identity=26.4214046822742, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24647658, Length=299, Percent_Identity=26.4214046822742, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B3_HALSA (Q9HHK5)

Other databases:

- EMBL:   AE004438
- RefSeq:   NP_395840.1
- ProteinModelPortal:   Q9HHK5
- GeneID:   1449350
- GenomeReviews:   AE004438_GR
- KEGG:   hal:VNG6351G
- NMPDR:   fig|64091.1.peg.2524
- HOGENOM:   HBG646536
- OMA:   YKRGLTS
- ProtClustDB:   CLSK511020
- BioCyc:   HSP64091:VNG6351G-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 35905; Mature: 35905

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERATREREKEQREQAQTNDEAQQCPECNSANVITDQSERVCEDCGLVLEDDQIDHGPEW
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCC
RAFNSSERDQKSRVGAPTTKTMHDKGLTTQIDWKDKDAYGRSLDAKKRNQMNRLRKWQER
CCCCCCHHHHHHHCCCCCCHHHHCCCCCEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHH
IRTKDAGERNLQFALSEIDRMASALGVPRSVREVASVIYRRALKEDLIRGRSIEGVATAC
HHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LYAACRQEGIPRTLEEVTEVARIDQKEIGRTYRYVAQELSLEIQPTDPKEYLPRFASDLE
HHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LSEEVIAKAREIIDTSAEQGLLSGKSPSGFAAAAIYAASLLCNEKKTQREVANVANVTEV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIRNRYQEQIEAMGFGV
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERATREREKEQREQAQTNDEAQQCPECNSANVITDQSERVCEDCGLVLEDDQIDHGPEW
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCC
RAFNSSERDQKSRVGAPTTKTMHDKGLTTQIDWKDKDAYGRSLDAKKRNQMNRLRKWQER
CCCCCCHHHHHHHCCCCCCHHHHCCCCCEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHH
IRTKDAGERNLQFALSEIDRMASALGVPRSVREVASVIYRRALKEDLIRGRSIEGVATAC
HHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LYAACRQEGIPRTLEEVTEVARIDQKEIGRTYRYVAQELSLEIQPTDPKEYLPRFASDLE
HHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LSEEVIAKAREIIDTSAEQGLLSGKSPSGFAAAAIYAASLLCNEKKTQREVANVANVTEV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIRNRYQEQIEAMGFGV
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11016950