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The map label for this gene is rlmN

Identifier: 160903334

GI number: 160903334

Start: 2111076

End: 2112119

Strand: Direct

Name: rlmN

Synonym: Pmob_1905

Alternate gene names: 160903334

Gene position: 2111076-2112119 (Clockwise)

Preceding gene: 160903333

Following gene: 160903335

Centisome position: 97.3

GC content: 33.81

Gene sequence:

>1044_bases
ATGCAAACAAAAAACATTTTAGATTTTGAATATTCAGACTTGCAAAGTTATTTGCTCAATGAATTGGGACTTGAAAAGTT
TAGAACTGATCAAATTTGTGATTGGATTTATAAGAAAAGAGTTTTTGATTTCGAATCGATGACTAATTTATCTAAAGACG
ATAGACAAAAACTCAGTGATAATTTTAAAATATCTATTCCTCATATTGTTAAAAAAGAGGTTTCAAAGATAGATGGAACC
ACCAAATATCTTTTGGAGTTAGAGGATAAGAACACTGTTGAAGCGGTAATTATTTACTACCCTTCAAGAACGATAGCTTG
TATATCAACACAAGTTGGTTGCCCACTGAAATGTTCTTTCTGCTCTACAGGACAAAGCGGTTACGTTAGAAATTTATCGA
CAGGTGAGATCATTGGTCAACTGTTAGCTATGGAAAAAGATAAAGAGATGGATGTAAAAAATGTTGTATACATGGGAATG
GGTGAACCATTATTAAATTTTAACAACGTTGTTCAAACCATAGAGATATTAAACCATCCAAAGATGAAAAAGTTAGGGGC
AAGGCATATAACCATTTCAACCGCGGGAATTCCTCAGAAAATTGAGGAAGTAGGTGATTTGAACAAAGAGTTTCGACTTT
CTGTTTCACTTCACGCACCAACGAATCTTCAAAGAGATCAAATAATGCCAATAAATCATAAATATCCGGTAGAGCAGGTT
ATTCAGTCATGCCGAATTTATCAGAAAAAAACAAAAAAGAGGGTTACTTTTGAGTATATTTTGATAAAAGGATTTAATGA
TTCAAAAGAGGACGCTTTGAAATTAGTTGAACTATTTGGTGATTTGAAAGTGATGGTTAATTTAATTCCTGTCAATGAAA
ATCCAGCGGGCTTTGAAAAGCCGTCAAAAAGGTTTATTCAAGCTTTTTTGGATACTCTCGTTAAGAACGGAATTGATGCT
GTGGTGCGAGCTGAGAAAGGTAGTGATATATCAGCCGCATGTGGCCAATTAAGAACTAGAGAGTTAAAAGAAGCTAATAG
GTGA

Upstream 100 bases:

>100_bases
GGCGGAGCCCTCCATCCCAGTTGGGTTAGGGACCGCAGGTCCCTCGTGGAGTATATGGAAGGGGAAACGATAGTATATAT
TTTAATGGAGAGGAAGTTAT

Downstream 100 bases:

>100_bases
ACATAATGATTAAAGTAAGAAAATTAATAAAAAATTTGATATTTTTTATTTTAGGGATAGTTGCCTCTGGAATATTTGCC
TTTTTTTTTATGACAGTTTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MQTKNILDFEYSDLQSYLLNELGLEKFRTDQICDWIYKKRVFDFESMTNLSKDDRQKLSDNFKISIPHIVKKEVSKIDGT
TKYLLELEDKNTVEAVIIYYPSRTIACISTQVGCPLKCSFCSTGQSGYVRNLSTGEIIGQLLAMEKDKEMDVKNVVYMGM
GEPLLNFNNVVQTIEILNHPKMKKLGARHITISTAGIPQKIEEVGDLNKEFRLSVSLHAPTNLQRDQIMPINHKYPVEQV
IQSCRIYQKKTKKRVTFEYILIKGFNDSKEDALKLVELFGDLKVMVNLIPVNENPAGFEKPSKRFIQAFLDTLVKNGIDA
VVRAEKGSDISAACGQLRTRELKEANR

Sequences:

>Translated_347_residues
MQTKNILDFEYSDLQSYLLNELGLEKFRTDQICDWIYKKRVFDFESMTNLSKDDRQKLSDNFKISIPHIVKKEVSKIDGT
TKYLLELEDKNTVEAVIIYYPSRTIACISTQVGCPLKCSFCSTGQSGYVRNLSTGEIIGQLLAMEKDKEMDVKNVVYMGM
GEPLLNFNNVVQTIEILNHPKMKKLGARHITISTAGIPQKIEEVGDLNKEFRLSVSLHAPTNLQRDQIMPINHKYPVEQV
IQSCRIYQKKTKKRVTFEYILIKGFNDSKEDALKLVELFGDLKVMVNLIPVNENPAGFEKPSKRFIQAFLDTLVKNGIDA
VVRAEKGSDISAACGQLRTRELKEANR
>Mature_347_residues
MQTKNILDFEYSDLQSYLLNELGLEKFRTDQICDWIYKKRVFDFESMTNLSKDDRQKLSDNFKISIPHIVKKEVSKIDGT
TKYLLELEDKNTVEAVIIYYPSRTIACISTQVGCPLKCSFCSTGQSGYVRNLSTGEIIGQLLAMEKDKEMDVKNVVYMGM
GEPLLNFNNVVQTIEILNHPKMKKLGARHITISTAGIPQKIEEVGDLNKEFRLSVSLHAPTNLQRDQIMPINHKYPVEQV
IQSCRIYQKKTKKRVTFEYILIKGFNDSKEDALKLVELFGDLKVMVNLIPVNENPAGFEKPSKRFIQAFLDTLVKNGIDA
VVRAEKGSDISAACGQLRTRELKEANR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=39.2441860465116, Blast_Score=236, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PETMO (A9BIC0)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568915.1
- GeneID:   5758088
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1905
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14469
- BioCyc:   PMOB403833:PMOB_1905-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39570; Mature: 39570

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKNILDFEYSDLQSYLLNELGLEKFRTDQICDWIYKKRVFDFESMTNLSKDDRQKLSD
CCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHCC
NFKISIPHIVKKEVSKIDGTTKYLLELEDKNTVEAVIIYYPSRTIACISTQVGCPLKCSF
CCEEECCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEECCCEEEEEECCCCCCEEEEE
CSTGQSGYVRNLSTGEIIGQLLAMEKDKEMDVKNVVYMGMGEPLLNFNNVVQTIEILNHP
CCCCCCCCEECCCHHHHHHHHHHHCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHCCC
KMKKLGARHITISTAGIPQKIEEVGDLNKEFRLSVSLHAPTNLQRDQIMPINHKYPVEQV
CHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHH
IQSCRIYQKKTKKRVTFEYILIKGFNDSKEDALKLVELFGDLKVMVNLIPVNENPAGFEK
HHHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCC
PSKRFIQAFLDTLVKNGIDAVVRAEKGSDISAACGQLRTRELKEANR
HHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTKNILDFEYSDLQSYLLNELGLEKFRTDQICDWIYKKRVFDFESMTNLSKDDRQKLSD
CCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHCC
NFKISIPHIVKKEVSKIDGTTKYLLELEDKNTVEAVIIYYPSRTIACISTQVGCPLKCSF
CCEEECCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEECCCEEEEEECCCCCCEEEEE
CSTGQSGYVRNLSTGEIIGQLLAMEKDKEMDVKNVVYMGMGEPLLNFNNVVQTIEILNHP
CCCCCCCCEECCCHHHHHHHHHHHCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHCCC
KMKKLGARHITISTAGIPQKIEEVGDLNKEFRLSVSLHAPTNLQRDQIMPINHKYPVEQV
CHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHH
IQSCRIYQKKTKKRVTFEYILIKGFNDSKEDALKLVELFGDLKVMVNLIPVNENPAGFEK
HHHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCC
PSKRFIQAFLDTLVKNGIDAVVRAEKGSDISAACGQLRTRELKEANR
HHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA