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The map label for this gene is amyB [H]

Identifier: 160903148

GI number: 160903148

Start: 1876890

End: 1878170

Strand: Direct

Name: amyB [H]

Synonym: Pmob_1713

Alternate gene names: 160903148

Gene position: 1876890-1878170 (Clockwise)

Preceding gene: 160903144

Following gene: 160903149

Centisome position: 86.51

GC content: 31.85

Gene sequence:

>1281_bases
GTGGCAATAGATTCACCAAGTTGGTTAAAAAGTTCTATAATTTATGAAGTTTTTGTTAGAAATTATGGAAATGCTGGAAC
CTTCAACGATATATATAACGATATTGAACGTATTAAGGCTTTGGGAGCAGATATACTTTGGTTTATGCCTTTTTATCCCA
TCGGAAAAGTTGGTAGAAAAGGTACACATGGGAGTCCCTATTCAATAAAAGATTACGAAGAAATATCGAAAGAAATTGGT
AATAAGAGGGATTTTAAAAGATTAATAGACAAAGCACATGATAACGGATTAAAAATAATGATCGATATTGTTTTCAATCA
CACCGCTATTGATTCGAAGCTAGCTGAAACACATCCTGAATGGTTTATGAAAGATCAAAACGGAAAATTAACTAGAAAGG
TAGAAGATTGGAACGACGTTTACGATTTGGATTATGATAATTTAGATTTATGGGATTATCTAATAAATGTTTTAGATGGT
TGGATTGATCTTGGTGTAGATGCTTTTAGATGTGATGTCGCTTCGCTAGTGCCTTTAGAGTTTTGGAAAAAATCTAGAGA
ACTGTTGAATCAAAAAAAGGAAATTATCTGGTTGGCTGAAACACTTGACCCGAAATTTATTCATGATCTCAGAACTAAAG
GGTACGATGTTCACTCGGACTCTGAGGTATATCAAGCTTTTGACCTTACATACGATTACGATGGCTTCTATTATCTAAGG
AGCTATTTTTCTGGAGAGAAAGATTTGAAGCATTTTTTTGAACATGTTTTTTTGCAGGAAACTATATTCCCAAAAAATTC
TCTTAAGTTGCGTTTTTTAGAAAATCATGATAATCCTAGAATAGCATCCGTTTTAGAGGGAAAAAATAAGATTAAAAATT
GGACTGTTTTATATAATCTTTTGCCAGGGGCATCTTTAATATATGCAGGCCAAGAATTAATGATGGAAAAACTGCCTAAC
CTTTTTGAGAAAGATTCAATCAAATGGGACAAAGGTGATTATGAATTTTTAAGTTTTTTCAAAAAAATCATTAAATTGAC
TAAAGAGATTAAATCTACTTGTAATCAATTCTCTCTTTGCGAGTTATCTGAGGGTATAGTAATGATAAAATGGAATGGAG
AAGAGGAGGAATACATTACCATTTTGAATTTGGAAGATAGACACGGAAAAATACCAGTAGATTTTAATTTATATGGAACA
GACCTTATAACGAATGAAACTGTTTCAATACAAAACTACTTTGTGATCAGCAAGTTACCTATGATTATAAAAGTCAGGTA
A

Upstream 100 bases:

>100_bases
ATTTTAAAAAAAGGGATAGGTATTTGATATAATCGATGATATAATACTTATTAGAATAATCGGGATGCAAAGAAAAATTT
CACTTCGAGGAGGTCCAATC

Downstream 100 bases:

>100_bases
AACAGCTAATAATTCAAATAAACCTTTGATTGATTTAATGGAGGTAGATGAATGGGAATTTTTGAAAAGTTCAAAAATGG
TTTAAGTAAGGCGAGAAACA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAIDSPSWLKSSIIYEVFVRNYGNAGTFNDIYNDIERIKALGADILWFMPFYPIGKVGRKGTHGSPYSIKDYEEISKEIG
NKRDFKRLIDKAHDNGLKIMIDIVFNHTAIDSKLAETHPEWFMKDQNGKLTRKVEDWNDVYDLDYDNLDLWDYLINVLDG
WIDLGVDAFRCDVASLVPLEFWKKSRELLNQKKEIIWLAETLDPKFIHDLRTKGYDVHSDSEVYQAFDLTYDYDGFYYLR
SYFSGEKDLKHFFEHVFLQETIFPKNSLKLRFLENHDNPRIASVLEGKNKIKNWTVLYNLLPGASLIYAGQELMMEKLPN
LFEKDSIKWDKGDYEFLSFFKKIIKLTKEIKSTCNQFSLCELSEGIVMIKWNGEEEEYITILNLEDRHGKIPVDFNLYGT
DLITNETVSIQNYFVISKLPMIIKVR

Sequences:

>Translated_426_residues
MAIDSPSWLKSSIIYEVFVRNYGNAGTFNDIYNDIERIKALGADILWFMPFYPIGKVGRKGTHGSPYSIKDYEEISKEIG
NKRDFKRLIDKAHDNGLKIMIDIVFNHTAIDSKLAETHPEWFMKDQNGKLTRKVEDWNDVYDLDYDNLDLWDYLINVLDG
WIDLGVDAFRCDVASLVPLEFWKKSRELLNQKKEIIWLAETLDPKFIHDLRTKGYDVHSDSEVYQAFDLTYDYDGFYYLR
SYFSGEKDLKHFFEHVFLQETIFPKNSLKLRFLENHDNPRIASVLEGKNKIKNWTVLYNLLPGASLIYAGQELMMEKLPN
LFEKDSIKWDKGDYEFLSFFKKIIKLTKEIKSTCNQFSLCELSEGIVMIKWNGEEEEYITILNLEDRHGKIPVDFNLYGT
DLITNETVSIQNYFVISKLPMIIKVR
>Mature_425_residues
AIDSPSWLKSSIIYEVFVRNYGNAGTFNDIYNDIERIKALGADILWFMPFYPIGKVGRKGTHGSPYSIKDYEEISKEIGN
KRDFKRLIDKAHDNGLKIMIDIVFNHTAIDSKLAETHPEWFMKDQNGKLTRKVEDWNDVYDLDYDNLDLWDYLINVLDGW
IDLGVDAFRCDVASLVPLEFWKKSRELLNQKKEIIWLAETLDPKFIHDLRTKGYDVHSDSEVYQAFDLTYDYDGFYYLRS
YFSGEKDLKHFFEHVFLQETIFPKNSLKLRFLENHDNPRIASVLEGKNKIKNWTVLYNLLPGASLIYAGQELMMEKLPNL
FEKDSIKWDKGDYEFLSFFKKIIKLTKEIKSTCNQFSLCELSEGIVMIKWNGEEEEYITILNLEDRHGKIPVDFNLYGTD
LITNETVSIQNYFVISKLPMIIKVR

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=383, Percent_Identity=22.7154046997389, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790687, Length=382, Percent_Identity=22.7748691099476, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321726, Length=397, Percent_Identity=24.9370277078086, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=467, Percent_Identity=24.1970021413276, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=216, Percent_Identity=28.2407407407407, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=216, Percent_Identity=28.2407407407407, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=217, Percent_Identity=27.6497695852535, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=217, Percent_Identity=27.6497695852535, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=142, Percent_Identity=28.8732394366197, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45549022, Length=403, Percent_Identity=23.3250620347395, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586599, Length=222, Percent_Identity=27.4774774774775, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 50043; Mature: 49911

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDSPSWLKSSIIYEVFVRNYGNAGTFNDIYNDIERIKALGADILWFMPFYPIGKVGRK
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCHHCCCC
GTHGSPYSIKDYEEISKEIGNKRDFKRLIDKAHDNGLKIMIDIVFNHTAIDSKLAETHPE
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCH
WFMKDQNGKLTRKVEDWNDVYDLDYDNLDLWDYLINVLDGWIDLGVDAFRCDVASLVPLE
HEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FWKKSRELLNQKKEIIWLAETLDPKFIHDLRTKGYDVHSDSEVYQAFDLTYDYDGFYYLR
HHHHHHHHHCCCHHEEEEEHHCCHHHHHHHHHCCCCCCCCCHHHHHEECEECCCHHHHHH
SYFSGEKDLKHFFEHVFLQETIFPKNSLKLRFLENHDNPRIASVLEGKNKIKNWTVLYNL
HHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCHHHHCCEEEEEEC
LPGASLIYAGQELMMEKLPNLFEKDSIKWDKGDYEFLSFFKKIIKLTKEIKSTCNQFSLC
CCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
ELSEGIVMIKWNGEEEEYITILNLEDRHGKIPVDFNLYGTDLITNETVSIQNYFVISKLP
ECCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEECCEEECCCEEEEEEEEEEECCC
MIIKVR
EEEEEC
>Mature Secondary Structure 
AIDSPSWLKSSIIYEVFVRNYGNAGTFNDIYNDIERIKALGADILWFMPFYPIGKVGRK
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCHHCCCC
GTHGSPYSIKDYEEISKEIGNKRDFKRLIDKAHDNGLKIMIDIVFNHTAIDSKLAETHPE
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCH
WFMKDQNGKLTRKVEDWNDVYDLDYDNLDLWDYLINVLDGWIDLGVDAFRCDVASLVPLE
HEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FWKKSRELLNQKKEIIWLAETLDPKFIHDLRTKGYDVHSDSEVYQAFDLTYDYDGFYYLR
HHHHHHHHHCCCHHEEEEEHHCCHHHHHHHHHCCCCCCCCCHHHHHEECEECCCHHHHHH
SYFSGEKDLKHFFEHVFLQETIFPKNSLKLRFLENHDNPRIASVLEGKNKIKNWTVLYNL
HHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCHHHHCCEEEEEEC
LPGASLIYAGQELMMEKLPNLFEKDSIKWDKGDYEFLSFFKKIIKLTKEIKSTCNQFSLC
CCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
ELSEGIVMIKWNGEEEEYITILNLEDRHGKIPVDFNLYGTDLITNETVSIQNYFVISKLP
ECCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEECCEEECCCEEEEEEEEEEECCC
MIIKVR
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2458257 [H]