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The map label for this gene is dosC [H]

Identifier: 160903105

GI number: 160903105

Start: 1827133

End: 1828632

Strand: Direct

Name: dosC [H]

Synonym: Pmob_1669

Alternate gene names: 160903105

Gene position: 1827133-1828632 (Clockwise)

Preceding gene: 160903104

Following gene: 160903107

Centisome position: 84.22

GC content: 29.87

Gene sequence:

>1500_bases
GTGGGTGCAGATTACGGAGATGTGAATAGAATGAATATAAAATTGCTTGAAAAGTATCAATCAAAAATTAATTCAGCTTA
TGTAAACCCAGGTATTACACTTTTACTTGAAAACTTCACAAAAGAAGTTCTTTTTGATTTTGAAGTTACAGTTAAAATTC
ATTCAGATCCACTTCAAGTACCTGATAATCTGTATAAATTAATAAAGATATTTAATTCATTCTCTATTTTCACGATAAAA
AATATAGAAGAGACTAATTTACTAGAAGAACAAAAAGTTGAATTGAAAAGTTTTCAAATGGAGAATATCATTGAAATTAA
TTACGAAACACTCAAAAAAGAGGAAAGTATGGCTTTGTATGAGACAATTAAAAATGCCTCTTCGTTAGTAGGATCAATGC
TTACCGATATTCTACTCAACTCTTCAAAAATAGCCAATGTATTCAGGGCTTCAAGAATTATATTAGAAAAAGGGTATAAA
CCTGGAACTTCTATCGATGAAATGATATATATAACTATGACAGCGATAACAGGTGGCTTTGCAGGAGGATTTAACAGGGC
TATCTTATTTGTTGAAGACAGTGGTGATTTCAAGGTTCATAGAGTTATAGGACCTGAAAATGAAATGGAAGCCCACAAAA
CTTATGAAAAGTTTGAAACACTTGAAACAAACATTGATTCATATTTGTCTCAATATGAGTTTGGAATGAGTTATTTTTCA
AATTTAGAAAAAAGGATAAAAGGTATTATTATTAAAAAAGAATTGTTAATAGATAATGATCTTTTTAAATACGCAGTTGA
ATCGCATAGCACTATTAAATTACCTGTAAGTCGATTGGAGGAAAAGATAGTAAATTTACTTGATTTAAGAGGAGAAATAG
CTATTTCACCAATAGAAATAGAGGATGAAAAACTTGCGTTTTATCTTTGCGATAATAGATACAACGGAAAACCAATTACA
GATGATCAAATCGAGATTCTAGATTATTATGCCAAAGAAAGTGGTGTGATGTGGCAAAACAAAATTTATCAGAGTTTATT
AAAAAAAGATGCTCAGATAGATTCTCTTACAGAGATTGGAAACAGAAGAAGTTACGAAAATTATATTTCTTCTATAAAAT
ATCTAAAAAATCAGAAAATAGCCTTAGTTGTTCTTGATTTAGACAACTTCAAAAAGGTAAACGATACATATGGCCACGAA
GAAGGAGACTCACTCTTAAAAAAATTTGCGCAACTATGTGTCGATAATTTGAGAAAAAAAGATAATATATTCAGATATGG
AGGCGATGAATTCGTAATAATTTTAGGAGGAGTTAAAAAAGAAGAGGTTTATTCTATTTTGGAAAGAATAAACAGCAAGC
TAAAACAAGAAACAAACGTTACTTTTTCAGCTGGAGTCGCCTACGGTGAAAGCCATGAAATAGATTCACTTTTCAATATA
GCAGATGAAAATTTATACATTGCAAAAGGGCAAGGGAAAAATAATATTATCATCACTTGA

Upstream 100 bases:

>100_bases
TGTCTCGGTTGAATAAATTGAACAGACATGTAGAATCTTTAACAAAAACTAATGGTGAGTTGATTACACCAGATGAAATA
TTAGACGTAATTATGAAAAT

Downstream 100 bases:

>100_bases
AATTTTATGCTGTTGCCACGTATTTTAAAATGAAAATCTTATTTTTAAAAAACTTTTTTAACCTAAGGCTACATCGAGAA
CCAACATAAGCAAAAATCCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MGADYGDVNRMNIKLLEKYQSKINSAYVNPGITLLLENFTKEVLFDFEVTVKIHSDPLQVPDNLYKLIKIFNSFSIFTIK
NIEETNLLEEQKVELKSFQMENIIEINYETLKKEESMALYETIKNASSLVGSMLTDILLNSSKIANVFRASRIILEKGYK
PGTSIDEMIYITMTAITGGFAGGFNRAILFVEDSGDFKVHRVIGPENEMEAHKTYEKFETLETNIDSYLSQYEFGMSYFS
NLEKRIKGIIIKKELLIDNDLFKYAVESHSTIKLPVSRLEEKIVNLLDLRGEIAISPIEIEDEKLAFYLCDNRYNGKPIT
DDQIEILDYYAKESGVMWQNKIYQSLLKKDAQIDSLTEIGNRRSYENYISSIKYLKNQKIALVVLDLDNFKKVNDTYGHE
EGDSLLKKFAQLCVDNLRKKDNIFRYGGDEFVIILGGVKKEEVYSILERINSKLKQETNVTFSAGVAYGESHEIDSLFNI
ADENLYIAKGQGKNNIIIT

Sequences:

>Translated_499_residues
MGADYGDVNRMNIKLLEKYQSKINSAYVNPGITLLLENFTKEVLFDFEVTVKIHSDPLQVPDNLYKLIKIFNSFSIFTIK
NIEETNLLEEQKVELKSFQMENIIEINYETLKKEESMALYETIKNASSLVGSMLTDILLNSSKIANVFRASRIILEKGYK
PGTSIDEMIYITMTAITGGFAGGFNRAILFVEDSGDFKVHRVIGPENEMEAHKTYEKFETLETNIDSYLSQYEFGMSYFS
NLEKRIKGIIIKKELLIDNDLFKYAVESHSTIKLPVSRLEEKIVNLLDLRGEIAISPIEIEDEKLAFYLCDNRYNGKPIT
DDQIEILDYYAKESGVMWQNKIYQSLLKKDAQIDSLTEIGNRRSYENYISSIKYLKNQKIALVVLDLDNFKKVNDTYGHE
EGDSLLKKFAQLCVDNLRKKDNIFRYGGDEFVIILGGVKKEEVYSILERINSKLKQETNVTFSAGVAYGESHEIDSLFNI
ADENLYIAKGQGKNNIIIT
>Mature_498_residues
GADYGDVNRMNIKLLEKYQSKINSAYVNPGITLLLENFTKEVLFDFEVTVKIHSDPLQVPDNLYKLIKIFNSFSIFTIKN
IEETNLLEEQKVELKSFQMENIIEINYETLKKEESMALYETIKNASSLVGSMLTDILLNSSKIANVFRASRIILEKGYKP
GTSIDEMIYITMTAITGGFAGGFNRAILFVEDSGDFKVHRVIGPENEMEAHKTYEKFETLETNIDSYLSQYEFGMSYFSN
LEKRIKGIIIKKELLIDNDLFKYAVESHSTIKLPVSRLEEKIVNLLDLRGEIAISPIEIEDEKLAFYLCDNRYNGKPITD
DQIEILDYYAKESGVMWQNKIYQSLLKKDAQIDSLTEIGNRRSYENYISSIKYLKNQKIALVVLDLDNFKKVNDTYGHEE
GDSLLKKFAQLCVDNLRKKDNIFRYGGDEFVIILGGVKKEEVYSILERINSKLKQETNVTFSAGVAYGESHEIDSLFNIA
DENLYIAKGQGKNNIIIT

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=33.1360946745562, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=34.7826086956522, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=31.2138728323699, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=32.7272727272727, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=29.608938547486, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787056, Length=152, Percent_Identity=36.1842105263158, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=32.3353293413174, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786584, Length=153, Percent_Identity=31.3725490196078, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=120, Percent_Identity=35, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=32.4022346368715, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=214, Percent_Identity=26.1682242990654, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787802, Length=168, Percent_Identity=29.7619047619048, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57192; Mature: 57061

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00583 PFKB_KINASES_1 ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGADYGDVNRMNIKLLEKYQSKINSAYVNPGITLLLENFTKEVLFDFEVTVKIHSDPLQV
CCCCCCCCCCHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHEEEEEEEEEECCCCCCC
PDNLYKLIKIFNSFSIFTIKNIEETNLLEEQKVELKSFQMENIIEINYETLKKEESMALY
CHHHHHHHHHHCCCCEEEECCCCHHCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHH
ETIKNASSLVGSMLTDILLNSSKIANVFRASRIILEKGYKPGTSIDEMIYITMTAITGGF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEHHCCC
AGGFNRAILFVEDSGDFKVHRVIGPENEMEAHKTYEKFETLETNIDSYLSQYEFGMSYFS
CCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLEKRIKGIIIKKELLIDNDLFKYAVESHSTIKLPVSRLEEKIVNLLDLRGEIAISPIEI
HHHHHHHHEEEEHHHHHCCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEECCEEE
EDEKLAFYLCDNRYNGKPITDDQIEILDYYAKESGVMWQNKIYQSLLKKDAQIDSLTEIG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHC
NRRSYENYISSIKYLKNQKIALVVLDLDNFKKVNDTYGHEEGDSLLKKFAQLCVDNLRKK
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DNIFRYGGDEFVIILGGVKKEEVYSILERINSKLKQETNVTFSAGVAYGESHEIDSLFNI
CCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
ADENLYIAKGQGKNNIIIT
CCCCEEEEECCCCCCEEEC
>Mature Secondary Structure 
GADYGDVNRMNIKLLEKYQSKINSAYVNPGITLLLENFTKEVLFDFEVTVKIHSDPLQV
CCCCCCCCCHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHEEEEEEEEEECCCCCCC
PDNLYKLIKIFNSFSIFTIKNIEETNLLEEQKVELKSFQMENIIEINYETLKKEESMALY
CHHHHHHHHHHCCCCEEEECCCCHHCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHH
ETIKNASSLVGSMLTDILLNSSKIANVFRASRIILEKGYKPGTSIDEMIYITMTAITGGF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEHHCCC
AGGFNRAILFVEDSGDFKVHRVIGPENEMEAHKTYEKFETLETNIDSYLSQYEFGMSYFS
CCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLEKRIKGIIIKKELLIDNDLFKYAVESHSTIKLPVSRLEEKIVNLLDLRGEIAISPIEI
HHHHHHHHEEEEHHHHHCCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEECCEEE
EDEKLAFYLCDNRYNGKPITDDQIEILDYYAKESGVMWQNKIYQSLLKKDAQIDSLTEIG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHC
NRRSYENYISSIKYLKNQKIALVVLDLDNFKKVNDTYGHEEGDSLLKKFAQLCVDNLRKK
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DNIFRYGGDEFVIILGGVKKEEVYSILERINSKLKQETNVTFSAGVAYGESHEIDSLFNI
CCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
ADENLYIAKGQGKNNIIIT
CCCCEEEEECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]