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The map label for this gene is ramA [H]

Identifier: 160903084

GI number: 160903084

Start: 1803443

End: 1804243

Strand: Direct

Name: ramA [H]

Synonym: Pmob_1648

Alternate gene names: 160903084

Gene position: 1803443-1804243 (Clockwise)

Preceding gene: 160903073

Following gene: 160903085

Centisome position: 83.13

GC content: 33.46

Gene sequence:

>801_bases
ATGTTTAAAGTTAGCGTAGTTCAATTCAAACCAGAACTATTTGAGATCGAGAAAAATGTAAATAAGGTAATGAAGATTAT
CGAAGGAGTCGATAGCAATTTTATAGTCTTTCCAGAATTAGCTTTTACCGGTTATGCTTTTTCCTCTAAAAAAGAAGTAG
AAGAAACTTACGAGTCGCCTTTAGATGGGATCGGATACGCCTTTAAAACTTTTAAGGAATTTTCTAAAGATACTGGTGTA
AGTGTAGTATATGGTTTTAACGAGAAGTATGAAGGTAAGTATTATAATTCATCAATTTTGATCAAAAGTGATGGGACATA
TAAAATATATAGAAAGACTCACTTGTTTTTCAGAGAGAAACTGTTTTTTACCCCGGGGGACACGGGTTTTTGGGTGGATA
ATATTAACGGAATAAATGTTGGAGTTGCCATCTGTTTTGATTGGTACTTTCCGGAATCCTTTAGAACATTGGCTTTATTA
GGTGCAGATTTAATTTTGCATCCAGCCAATTTAGTCTTACCATATTGTCAAGAAGCCAATAAAATTAGATCTTTAGAAAA
CAAGATTTATATTGCCACATCAAATATTTGGGGAACCCAGATCAACGGTGAAATAGAGTATTCTTTCACGGGTAAAAGTC
AAGTGACTTCACCTGATGGAGAGGTGCTTTTTAGATTACCAGAGCAAGGTGATTTTATTCAAACAGCAGAAATAGATGAA
AGGAAATCTCAAAATAAACGTATTAATGAATACAACGATCTCTTCCAAGATCGAAAACCAAAATATTATTTTCACTCTTA
G

Upstream 100 bases:

>100_bases
TTATACAATAATTTTAAATAGTTGGCAAAAGTTTTCTTCTTTTTATTATATAATGTGCATTGGAGTAGAGTAAATGGGTG
AGTGAAGAGGAGGTTGAAAA

Downstream 100 bases:

>100_bases
ATTAAGAGTGATATAATTTTTGATTGCGTTTAAATTTAGCTAGCTTATAAGCACTTTATCATAGATATTATATGTATTCT
CTTAACTTAATAAATCACTT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MFKVSVVQFKPELFEIEKNVNKVMKIIEGVDSNFIVFPELAFTGYAFSSKKEVEETYESPLDGIGYAFKTFKEFSKDTGV
SVVYGFNEKYEGKYYNSSILIKSDGTYKIYRKTHLFFREKLFFTPGDTGFWVDNINGINVGVAICFDWYFPESFRTLALL
GADLILHPANLVLPYCQEANKIRSLENKIYIATSNIWGTQINGEIEYSFTGKSQVTSPDGEVLFRLPEQGDFIQTAEIDE
RKSQNKRINEYNDLFQDRKPKYYFHS

Sequences:

>Translated_266_residues
MFKVSVVQFKPELFEIEKNVNKVMKIIEGVDSNFIVFPELAFTGYAFSSKKEVEETYESPLDGIGYAFKTFKEFSKDTGV
SVVYGFNEKYEGKYYNSSILIKSDGTYKIYRKTHLFFREKLFFTPGDTGFWVDNINGINVGVAICFDWYFPESFRTLALL
GADLILHPANLVLPYCQEANKIRSLENKIYIATSNIWGTQINGEIEYSFTGKSQVTSPDGEVLFRLPEQGDFIQTAEIDE
RKSQNKRINEYNDLFQDRKPKYYFHS
>Mature_266_residues
MFKVSVVQFKPELFEIEKNVNKVMKIIEGVDSNFIVFPELAFTGYAFSSKKEVEETYESPLDGIGYAFKTFKEFSKDTGV
SVVYGFNEKYEGKYYNSSILIKSDGTYKIYRKTHLFFREKLFFTPGDTGFWVDNINGINVGVAICFDWYFPESFRTLALL
GADLILHPANLVLPYCQEANKIRSLENKIYIATSNIWGTQINGEIEYSFTGKSQVTSPDGEVLFRLPEQGDFIQTAEIDE
RKSQNKRINEYNDLFQDRKPKYYFHS

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 30791; Mature: 30791

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVSVVQFKPELFEIEKNVNKVMKIIEGVDSNFIVFPELAFTGYAFSSKKEVEETYESP
CEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHEECCCCCCHHHHHHHHHCC
LDGIGYAFKTFKEFSKDTGVSVVYGFNEKYEGKYYNSSILIKSDGTYKIYRKTHLFFREK
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCEEEEECCCCEEEEEHHHHEEEEE
LFFTPGDTGFWVDNINGINVGVAICFDWYFPESFRTLALLGADLILHPANLVLPYCQEAN
EEECCCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHCCHHEECCHHHHHHHHHHHH
KIRSLENKIYIATSNIWGTQINGEIEYSFTGKSQVTSPDGEVLFRLPEQGDFIQTAEIDE
HHHHHCCEEEEEECCCCCEEECCEEEEEECCCCCCCCCCCCEEEECCCCCCEEEECCCHH
RKSQNKRINEYNDLFQDRKPKYYFHS
HHHHCCCCHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MFKVSVVQFKPELFEIEKNVNKVMKIIEGVDSNFIVFPELAFTGYAFSSKKEVEETYESP
CEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHEECCCCCCHHHHHHHHHCC
LDGIGYAFKTFKEFSKDTGVSVVYGFNEKYEGKYYNSSILIKSDGTYKIYRKTHLFFREK
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCEEEEECCCCEEEEEHHHHEEEEE
LFFTPGDTGFWVDNINGINVGVAICFDWYFPESFRTLALLGADLILHPANLVLPYCQEAN
EEECCCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHCCHHEECCHHHHHHHHHHHH
KIRSLENKIYIATSNIWGTQINGEIEYSFTGKSQVTSPDGEVLFRLPEQGDFIQTAEIDE
HHHHHCCEEEEEECCCCCEEECCEEEEEECCCCCCCCCCCCEEEECCCCCCEEEECCCHH
RKSQNKRINEYNDLFQDRKPKYYFHS
HHHHCCCCHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA