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The map label for this gene is queA [H]

Identifier: 160902969

GI number: 160902969

Start: 1649717

End: 1650742

Strand: Direct

Name: queA [H]

Synonym: Pmob_1526

Alternate gene names: 160902969

Gene position: 1649717-1650742 (Clockwise)

Preceding gene: 160902968

Following gene: 160902970

Centisome position: 76.04

GC content: 37.04

Gene sequence:

>1026_bases
ATGAGTGAACAGAGCTTTGACTTAGAGCAGTATAACTACGAGTTACCTGAAGAGCTTATAGCCCAAGAACCTGTTGAACC
AAGAGATAGCTGCAAGCTGATGGTGCTAAATAGAAGAGTAAAAAGTATCGAACACAAAGTTTTTAGGGATATAAAAAATT
ATTTACGACCCGGCGATTTGCTTGTTTTAAACAATACCAGGGTTATCCCTGCAAGATTGTACGGCAAAAAGGAAACAGGT
GCTAAAGTTGAAGTTTTACTTTTAGAAAAAGATGGAAACGATAAAACATGGAAAGCTTTGGTAAAGCCAGGTGGAAAGAT
AAAAAAAGGCAACAAACTTATCTTTGAAGATGATTTAGTTTGCACAGTAGTAGAACATTTGGAAGACGGATCTAGAATTT
TAGAATTTGACGATCCCAACTTTTTCTCAAAATTGCCAAAAATTGGCGAAGTCCCTTTACCGCCTTATATTAAAAAACAG
ATCGATGATCCAGAAAAATATCAAACTACTTACGCAAAAGAAGATGGTGCCGTGGCTGCGCCAACCGCTGGTTTGCATTT
CACACAAGAGTTAATAGACGAGCTCACCGATTACGGTGTTAGATTTGCTGAAGTTACTCTTCATGTAGGTTTAGGAACCT
TTAGACCTGTTAGCGCGGCAGATATAAGGAACCACCAAATACATGAGGAGTATTATACCGTATCAAAGAGTGTGCTTCGC
GATATAGTTAGAGCTAAAGCCGAAGGTAAAAGGGTAATAGCCACAGGAACAACGGTTGTTAGAACGCTCGAATCAATTGC
CAGAAATCCAGACAAACTCGCTGGTAAAACAGATCTATATATTTATCCGCCTTTTGAATTTAAAATAATTGATGCTATGA
TCACCAATTTTCATCTACCGAAATCTTCACTTCTTTTTCTTGTTTCGGCATTTGCTGGTCAAGATTTTATTATGAACTCC
TATGAAATTGCTAAAGAAAAGAAATATAGGTTTTTTTCGTTTGGAGATGCTATGTTCATAATTTAA

Upstream 100 bases:

>100_bases
CATTTTAGTAATAAACTTGGTTATTACATGATTTCACAAGATAAATTTAGTGAATATCGGTTTGATAAAGACAAATTATT
GGAACTTCGGAGGTTTGTAG

Downstream 100 bases:

>100_bases
TTTCTATATTACACACTTTAGAAAAAATTTCAACATAATATTTAAATTTTAAAAGGGTCACAGGGTGGGTTAAAGAAAGT
ATTCTTTATATTAAAGTTTA

Product: S-adenosylmethionine--tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSEQSFDLEQYNYELPEELIAQEPVEPRDSCKLMVLNRRVKSIEHKVFRDIKNYLRPGDLLVLNNTRVIPARLYGKKETG
AKVEVLLLEKDGNDKTWKALVKPGGKIKKGNKLIFEDDLVCTVVEHLEDGSRILEFDDPNFFSKLPKIGEVPLPPYIKKQ
IDDPEKYQTTYAKEDGAVAAPTAGLHFTQELIDELTDYGVRFAEVTLHVGLGTFRPVSAADIRNHQIHEEYYTVSKSVLR
DIVRAKAEGKRVIATGTTVVRTLESIARNPDKLAGKTDLYIYPPFEFKIIDAMITNFHLPKSSLLFLVSAFAGQDFIMNS
YEIAKEKKYRFFSFGDAMFII

Sequences:

>Translated_341_residues
MSEQSFDLEQYNYELPEELIAQEPVEPRDSCKLMVLNRRVKSIEHKVFRDIKNYLRPGDLLVLNNTRVIPARLYGKKETG
AKVEVLLLEKDGNDKTWKALVKPGGKIKKGNKLIFEDDLVCTVVEHLEDGSRILEFDDPNFFSKLPKIGEVPLPPYIKKQ
IDDPEKYQTTYAKEDGAVAAPTAGLHFTQELIDELTDYGVRFAEVTLHVGLGTFRPVSAADIRNHQIHEEYYTVSKSVLR
DIVRAKAEGKRVIATGTTVVRTLESIARNPDKLAGKTDLYIYPPFEFKIIDAMITNFHLPKSSLLFLVSAFAGQDFIMNS
YEIAKEKKYRFFSFGDAMFII
>Mature_340_residues
SEQSFDLEQYNYELPEELIAQEPVEPRDSCKLMVLNRRVKSIEHKVFRDIKNYLRPGDLLVLNNTRVIPARLYGKKETGA
KVEVLLLEKDGNDKTWKALVKPGGKIKKGNKLIFEDDLVCTVVEHLEDGSRILEFDDPNFFSKLPKIGEVPLPPYIKKQI
DDPEKYQTTYAKEDGAVAAPTAGLHFTQELIDELTDYGVRFAEVTLHVGLGTFRPVSAADIRNHQIHEEYYTVSKSVLRD
IVRAKAEGKRVIATGTTVVRTLESIARNPDKLAGKTDLYIYPPFEFKIIDAMITNFHLPKSSLLFLVSAFAGQDFIMNSY
EIAKEKKYRFFSFGDAMFII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=339, Percent_Identity=46.9026548672566, Blast_Score=300, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38843; Mature: 38712

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQSFDLEQYNYELPEELIAQEPVEPRDSCKLMVLNRRVKSIEHKVFRDIKNYLRPGDL
CCCCCCCHHHCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
LVLNNTRVIPARLYGKKETGAKVEVLLLEKDGNDKTWKALVKPGGKIKKGNKLIFEDDLV
EEECCCEEEEHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCEECCCEEEEECCHH
CTVVEHLEDGSRILEFDDPNFFSKLPKIGEVPLPPYIKKQIDDPEKYQTTYAKEDGAVAA
HHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCCCCEEC
PTAGLHFTQELIDELTDYGVRFAEVTLHVGLGTFRPVSAADIRNHQIHEEYYTVSKSVLR
CCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
DIVRAKAEGKRVIATGTTVVRTLESIARNPDKLAGKTDLYIYPPFEFKIIDAMITNFHLP
HHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHCCCCCEEEECCCCEEHHHHHHHHCCCC
KSSLLFLVSAFAGQDFIMNSYEIAKEKKYRFFSFGDAMFII
HHHHHHHHHHHCCCCHHHCCHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure 
SEQSFDLEQYNYELPEELIAQEPVEPRDSCKLMVLNRRVKSIEHKVFRDIKNYLRPGDL
CCCCCCHHHCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
LVLNNTRVIPARLYGKKETGAKVEVLLLEKDGNDKTWKALVKPGGKIKKGNKLIFEDDLV
EEECCCEEEEHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCEECCCEEEEECCHH
CTVVEHLEDGSRILEFDDPNFFSKLPKIGEVPLPPYIKKQIDDPEKYQTTYAKEDGAVAA
HHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCCCCEEC
PTAGLHFTQELIDELTDYGVRFAEVTLHVGLGTFRPVSAADIRNHQIHEEYYTVSKSVLR
CCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
DIVRAKAEGKRVIATGTTVVRTLESIARNPDKLAGKTDLYIYPPFEFKIIDAMITNFHLP
HHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHCCCCCEEEECCCCEEHHHHHHHHCCCC
KSSLLFLVSAFAGQDFIMNSYEIAKEKKYRFFSFGDAMFII
HHHHHHHHHHHCCCCHHHCCHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]