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The map label for this gene is splB [H]

Identifier: 160902861

GI number: 160902861

Start: 1521593

End: 1522609

Strand: Direct

Name: splB [H]

Synonym: Pmob_1416

Alternate gene names: 160902861

Gene position: 1521593-1522609 (Clockwise)

Preceding gene: 160902860

Following gene: 160902865

Centisome position: 70.13

GC content: 31.76

Gene sequence:

>1017_bases
TTGAAGATCTCAAATCAAAACTATACAAGTGAAACCTTCTCACACATCTACATTGAAAAAGAAGCTTTAAATTACTCCCT
AACAAATGAAATTACTAGCAAGCTGAGCAAAAGTAAGATAGTATATATAGATAACTACAATGAAATATTCTCTAGAAAGA
ACCAAAACCCTTTTCTACAAAAACTCTCACTGTCTTTGATTCTAGCAGTTAACAAGGGTGATTTTATCTATAAAGGGTCT
CCTTTGTGCCATGACTTTGAACAACAAAATTTCTACTACACAAATTTCATAGTTAATTGTCTGTTCGATTGCGATTACTG
TTATTTGAAAGGTATGAACTTCTCTTCCCACATTTTATTCTTTGTTAATCTCTCAGATTACTTTCATGAATTAAGCAGAT
TGTTAAGTGAAGAAGGGAAGCTTTATCTAAGCATATCGTATGATTCAGATATCCTTCTTTTTGAAGGGATCTACCCATTT
TTAAAAGAATGGATAGAGTTTGCTAAGGCAAACCCTTTGATAACTATAGAAATTAGGACTAAAACAACCAATTATAAAAA
ATTTTTGAACTACTCTCCATTAGAAAATTTGATCATTACATGGAGTATATCCCCCCAAGAAGTAATAGCAAAATATGAAA
AAAACACCCCCTCTTTAGAAAGAAGGCTAATGGCTATATCTCAGTTACTACAAAAAGGTTGGAAAGTCAATTTAGCGTTG
GATCCTATACTTTATATAGGAGAAGATTGGGGAGAGAAATATAAGGCGTTCATTAACCAGATATCACAGAACATTAACTT
AAAGAAGATTAATGCGATTACACTGGGAGTATTCAGAATGCCGTCTGACTACTTAAAAAGATTTAAAAAGTTTTTTAAAT
CCCCGATTGCATTTTTCCCTTATCAAACCGCAAATGGTGTATCCACTTACCCTGAGGAATTAAAAAGTAAAATGATTGAA
TTCGTCTCTTCAGAGTTGGAAAAAATATATGATAAAAGAAACATATACATAGTTTGA

Upstream 100 bases:

>100_bases
GCATATGCCTATTTCAAATCTAATAAGAAAGTAAGCCCTTTATTCTTAGAAGAATATTTTAACTACATTCCAAAAAGCCA
AAAAGAAAGGGATGCGACTT

Downstream 100 bases:

>100_bases
TTGTTTTAGAAAAAAAGACGGACCAATGTCCGCCATTATTTAATGAATCAATTGATTAATTTTTTTACAAAAACAGGTAG
AGAAAAAGATGCAACGTGAA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKISNQNYTSETFSHIYIEKEALNYSLTNEITSKLSKSKIVYIDNYNEIFSRKNQNPFLQKLSLSLILAVNKGDFIYKGS
PLCHDFEQQNFYYTNFIVNCLFDCDYCYLKGMNFSSHILFFVNLSDYFHELSRLLSEEGKLYLSISYDSDILLFEGIYPF
LKEWIEFAKANPLITIEIRTKTTNYKKFLNYSPLENLIITWSISPQEVIAKYEKNTPSLERRLMAISQLLQKGWKVNLAL
DPILYIGEDWGEKYKAFINQISQNINLKKINAITLGVFRMPSDYLKRFKKFFKSPIAFFPYQTANGVSTYPEELKSKMIE
FVSSELEKIYDKRNIYIV

Sequences:

>Translated_338_residues
MKISNQNYTSETFSHIYIEKEALNYSLTNEITSKLSKSKIVYIDNYNEIFSRKNQNPFLQKLSLSLILAVNKGDFIYKGS
PLCHDFEQQNFYYTNFIVNCLFDCDYCYLKGMNFSSHILFFVNLSDYFHELSRLLSEEGKLYLSISYDSDILLFEGIYPF
LKEWIEFAKANPLITIEIRTKTTNYKKFLNYSPLENLIITWSISPQEVIAKYEKNTPSLERRLMAISQLLQKGWKVNLAL
DPILYIGEDWGEKYKAFINQISQNINLKKINAITLGVFRMPSDYLKRFKKFFKSPIAFFPYQTANGVSTYPEELKSKMIE
FVSSELEKIYDKRNIYIV
>Mature_338_residues
MKISNQNYTSETFSHIYIEKEALNYSLTNEITSKLSKSKIVYIDNYNEIFSRKNQNPFLQKLSLSLILAVNKGDFIYKGS
PLCHDFEQQNFYYTNFIVNCLFDCDYCYLKGMNFSSHILFFVNLSDYFHELSRLLSEEGKLYLSISYDSDILLFEGIYPF
LKEWIEFAKANPLITIEIRTKTTNYKKFLNYSPLENLIITWSISPQEVIAKYEKNTPSLERRLMAISQLLQKGWKVNLAL
DPILYIGEDWGEKYKAFINQISQNINLKKINAITLGVFRMPSDYLKRFKKFFKSPIAFFPYQTANGVSTYPEELKSKMIE
FVSSELEKIYDKRNIYIV

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39807; Mature: 39807

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISNQNYTSETFSHIYIEKEALNYSLTNEITSKLSKSKIVYIDNYNEIFSRKNQNPFLQ
CCCCCCCCCHHHHHEEEEEHHHCCCHHHHHHHHHHCCCEEEEEECCHHHHHCCCCCHHHH
KLSLSLILAVNKGDFIYKGSPLCHDFEQQNFYYTNFIVNCLFDCDYCYLKGMNFSSHILF
HHCEEEEEEECCCCEEECCCCCCCCHHHCCEEHHHHHHHHHHCCCCCEEECCCCCCEEEE
FVNLSDYFHELSRLLSEEGKLYLSISYDSDILLFEGIYPFLKEWIEFAKANPLITIEIRT
EEEHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEEE
KTTNYKKFLNYSPLENLIITWSISPQEVIAKYEKNTPSLERRLMAISQLLQKGWKVNLAL
CCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEE
DPILYIGEDWGEKYKAFINQISQNINLKKINAITLGVFRMPSDYLKRFKKFFKSPIAFFP
CCEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCHHHHHHHHHHHCCCCEECC
YQTANGVSTYPEELKSKMIEFVSSELEKIYDKRNIYIV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MKISNQNYTSETFSHIYIEKEALNYSLTNEITSKLSKSKIVYIDNYNEIFSRKNQNPFLQ
CCCCCCCCCHHHHHEEEEEHHHCCCHHHHHHHHHHCCCEEEEEECCHHHHHCCCCCHHHH
KLSLSLILAVNKGDFIYKGSPLCHDFEQQNFYYTNFIVNCLFDCDYCYLKGMNFSSHILF
HHCEEEEEEECCCCEEECCCCCCCCHHHCCEEHHHHHHHHHHCCCCCEEECCCCCCEEEE
FVNLSDYFHELSRLLSEEGKLYLSISYDSDILLFEGIYPFLKEWIEFAKANPLITIEIRT
EEEHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEEE
KTTNYKKFLNYSPLENLIITWSISPQEVIAKYEKNTPSLERRLMAISQLLQKGWKVNLAL
CCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEE
DPILYIGEDWGEKYKAFINQISQNINLKKINAITLGVFRMPSDYLKRFKKFFKSPIAFFP
CCEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCHHHHHHHHHHHCCCCEECC
YQTANGVSTYPEELKSKMIEFVSSELEKIYDKRNIYIV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]