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The map label for this gene is miaA

Identifier: 160902826

GI number: 160902826

Start: 1487973

End: 1488884

Strand: Direct

Name: miaA

Synonym: Pmob_1380

Alternate gene names: 160902826

Gene position: 1487973-1488884 (Clockwise)

Preceding gene: 160902825

Following gene: 160902827

Centisome position: 68.58

GC content: 33.11

Gene sequence:

>912_bases
GTGAATAAAGTTTTAGTTATAGCAGGTCCTACGGCTGTCGGAAAAACAGAAATATCCATTGAGATAGCCAGAAGAATTAA
TGGTGAAATAATTTGTATGGATTCGAGACAAATTTATAGCCATCTTATAATTGGTACCGCTACGCCTGATGAAGAAACAA
AAAAATTGGTCCCACATCACTTGTATGGTTCAGTAGATCCACGGACCCATTTTACAGCTTTTGATTATAAAAAATTGGCA
GAAAAAAAAATCGGTGAAGTACTAAATAGAGGAAATACACCAGTTTTAGTCGGCGGGACAGGCCTATACCTTGATGCTCT
GAGGAAAGGTTTTTTGAATGTAAAATCCGACTATGGTTTAAGGACTTATTTAAGAAAATTAGAAACAAATAATCCTGGTG
TTTTAAGAAAGATTTTGGTAGATTTGGATCCACAAAGAGCTCAAAAGATTCATCCAAACGACTTGAAAAGGATCATTAGG
GCCATAGAGATTTATGTTATAACAGGTATAAAAATGGGTGAAATTGTAAAAGAAAATAGGCAAGACGAAAATTCTTTTGA
TTACCATATAATAGTACTGGATAGAGAAAGGCAAGAATTGCACGAAAGGATTAACAAAAGAGTTCATCAAATGATCGATG
AAGGTTTGATAGAAGAGGTGCGAAATCTCTTGTCTTTAGGGTATTCTACAACTCTTAACGCTTTAAACACCATAGGTTAT
AAAGAAGTGGTACAATATTTATATGGAAAAATTGATTTTAATGAAATGGTTCACCAAATAAAAGTAAATACTCGTAACTA
TGCCAGAAGACAGATAATATACTTTCGTAAGATAGAAGGTGCAAAGTGGATCAATTTGAGTAAAACAAGTCAAGAAGAGG
TAGTAGATCAAATATTAAGTGAATTTATTTAA

Upstream 100 bases:

>100_bases
TAATCAAAGCACGTTCTAACGACAGTGTATGGGATTTATACAGTTTCGATAAAAAAATCGGAAGTAAAAAAAGTGGAGTA
TTTTTCGGAGTGATTGAAAA

Downstream 100 bases:

>100_bases
TTTCTTGTGCGAGGGGTGATAACATGGCAGAAAAGTTCAATTTACAAGACCGATTTTTGAATATTTTGAGGATTAACAAG
ATAGAAGTAAAAATCTATTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNKVLVIAGPTAVGKTEISIEIARRINGEIICMDSRQIYSHLIIGTATPDEETKKLVPHHLYGSVDPRTHFTAFDYKKLA
EKKIGEVLNRGNTPVLVGGTGLYLDALRKGFLNVKSDYGLRTYLRKLETNNPGVLRKILVDLDPQRAQKIHPNDLKRIIR
AIEIYVITGIKMGEIVKENRQDENSFDYHIIVLDRERQELHERINKRVHQMIDEGLIEEVRNLLSLGYSTTLNALNTIGY
KEVVQYLYGKIDFNEMVHQIKVNTRNYARRQIIYFRKIEGAKWINLSKTSQEEVVDQILSEFI

Sequences:

>Translated_303_residues
MNKVLVIAGPTAVGKTEISIEIARRINGEIICMDSRQIYSHLIIGTATPDEETKKLVPHHLYGSVDPRTHFTAFDYKKLA
EKKIGEVLNRGNTPVLVGGTGLYLDALRKGFLNVKSDYGLRTYLRKLETNNPGVLRKILVDLDPQRAQKIHPNDLKRIIR
AIEIYVITGIKMGEIVKENRQDENSFDYHIIVLDRERQELHERINKRVHQMIDEGLIEEVRNLLSLGYSTTLNALNTIGY
KEVVQYLYGKIDFNEMVHQIKVNTRNYARRQIIYFRKIEGAKWINLSKTSQEEVVDQILSEFI
>Mature_303_residues
MNKVLVIAGPTAVGKTEISIEIARRINGEIICMDSRQIYSHLIIGTATPDEETKKLVPHHLYGSVDPRTHFTAFDYKKLA
EKKIGEVLNRGNTPVLVGGTGLYLDALRKGFLNVKSDYGLRTYLRKLETNNPGVLRKILVDLDPQRAQKIHPNDLKRIIR
AIEIYVITGIKMGEIVKENRQDENSFDYHIIVLDRERQELHERINKRVHQMIDEGLIEEVRNLLSLGYSTTLNALNTIGY
KEVVQYLYGKIDFNEMVHQIKVNTRNYARRQIIYFRKIEGAKWINLSKTSQEEVVDQILSEFI

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=301, Percent_Identity=29.2358803986711, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1790613, Length=303, Percent_Identity=34.3234323432343, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=26.7326732673267, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=32.7586206896552, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=34.7169811320755, Blast_Score=144, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PETMO (A9BHW9)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568407.1
- ProteinModelPortal:   A9BHW9
- SMR:   A9BHW9
- GeneID:   5756673
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1380
- HOGENOM:   HBG559459
- OMA:   KEVRHAI
- BioCyc:   PMOB403833:PMOB_1380-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34943; Mature: 34943

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLVIAGPTAVGKTEISIEIARRINGEIICMDSRQIYSHLIIGTATPDEETKKLVPHH
CCCEEEEECCCCCCCCEEEEEEHHHCCCEEEEEECHHHHHHEEEECCCCCHHHHHHCCHH
LYGSVDPRTHFTAFDYKKLAEKKIGEVLNRGNTPVLVGGTGLYLDALRKGFLNVKSDYGL
HCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCCH
RTYLRKLETNNPGVLRKILVDLDPQRAQKIHPNDLKRIIRAIEIYVITGIKMGEIVKENR
HHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHEECCCHHHHHHHCC
QDENSFDYHIIVLDRERQELHERINKRVHQMIDEGLIEEVRNLLSLGYSTTLNALNTIGY
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
KEVVQYLYGKIDFNEMVHQIKVNTRNYARRQIIYFRKIEGAKWINLSKTSQEEVVDQILS
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHH
EFI
HCC
>Mature Secondary Structure
MNKVLVIAGPTAVGKTEISIEIARRINGEIICMDSRQIYSHLIIGTATPDEETKKLVPHH
CCCEEEEECCCCCCCCEEEEEEHHHCCCEEEEEECHHHHHHEEEECCCCCHHHHHHCCHH
LYGSVDPRTHFTAFDYKKLAEKKIGEVLNRGNTPVLVGGTGLYLDALRKGFLNVKSDYGL
HCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCCH
RTYLRKLETNNPGVLRKILVDLDPQRAQKIHPNDLKRIIRAIEIYVITGIKMGEIVKENR
HHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHEECCCHHHHHHHCC
QDENSFDYHIIVLDRERQELHERINKRVHQMIDEGLIEEVRNLLSLGYSTTLNALNTIGY
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
KEVVQYLYGKIDFNEMVHQIKVNTRNYARRQIIYFRKIEGAKWINLSKTSQEEVVDQILS
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHH
EFI
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA