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The map label for this gene is punA [H]

Identifier: 160902049

GI number: 160902049

Start: 624803

End: 625615

Strand: Direct

Name: punA [H]

Synonym: Pmob_0573

Alternate gene names: 160902049

Gene position: 624803-625615 (Clockwise)

Preceding gene: 160902048

Following gene: 160902050

Centisome position: 28.8

GC content: 34.81

Gene sequence:

>813_bases
ATGACCACTAAAATCGAAAAAGCTTCTCAATATATTAAAGAAAGTACAGAAATAAAACCAATTTTAGGATTAATATTAGG
CTCTGGCCTTGGATACATAGCGGACCAGGTAGAAAATCCAAAGGTGATTGAATACAAGGACATTCCTTTTTTTCCACAAT
CAACTGTAGTAGGACATGAAGGGTCTTTAGTCATAGGTACTATAGAAGGTATCCCTGTCATGATATTAAAAGGACGATTT
CATGCTTATGAAGGAATTGAACTAAAAGATATTGTTTTTCCTATTTATGTGATGAAGGATTTAGGAGTTAAAGGTCTGAT
AATAACGAATGCAGCTGGTGGAATTAACAAAACATTTTCTCCAGGTGATATAGTGGTAGACGTCGATTTCATAAATTTTA
CATTCAAAAATCCTTTAAGAGGCCCTAATTTAGATGAGTTTGGCCCCAGATTCCCTTCTTTAGTTGAGCCTGTCGATAAA
AATTGGGTAAAAAGCGTTATCGAGAAATGTAAAAAAGATAATATAGAGCTCAAAGAGGGGACTTATTTATGGACTTTGGG
TCCATCTTATGAAACCCCTTCTGAAATTAGAATGTTCGACAAATATGAAGCAGACCTAGTAGGAATGTCAACCTTACCAG
AAGTGATGGCGGCGAATCATGTAGGCTTAAAAGTTATCTCATTTTCTGCAGTTACCAATATGGCTGCTGGTATTCTCCCA
CAACCTCTAAAACATGAAGATGTTCTTAGAATAACTGAAAAAATAAAGGGAAAGTTTGAAAAGGTCGTATACAACGCTAT
TAAATTATTTTAA

Upstream 100 bases:

>100_bases
TCTTACGTCACATGAATCAAAGCATATAATAATAGGAAATCTAAATTAAAGTTTAACTCTTTAAATTTATTTTTTGCCCT
AAGGAGGTACATATTTACAT

Downstream 100 bases:

>100_bases
TATAAAACATCAAAAAGAACGGAGGACAAAAGTTGTCTGAAAATCTAAAAGAGATTGTAGAAGAGATAAGTAAAAATGAT
AATATTTTGATAGTAGGCCA

Product: purine nucleoside phosphorylase I, inosine and guanosine-specific

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTTKIEKASQYIKESTEIKPILGLILGSGLGYIADQVENPKVIEYKDIPFFPQSTVVGHEGSLVIGTIEGIPVMILKGRF
HAYEGIELKDIVFPIYVMKDLGVKGLIITNAAGGINKTFSPGDIVVDVDFINFTFKNPLRGPNLDEFGPRFPSLVEPVDK
NWVKSVIEKCKKDNIELKEGTYLWTLGPSYETPSEIRMFDKYEADLVGMSTLPEVMAANHVGLKVISFSAVTNMAAGILP
QPLKHEDVLRITEKIKGKFEKVVYNAIKLF

Sequences:

>Translated_270_residues
MTTKIEKASQYIKESTEIKPILGLILGSGLGYIADQVENPKVIEYKDIPFFPQSTVVGHEGSLVIGTIEGIPVMILKGRF
HAYEGIELKDIVFPIYVMKDLGVKGLIITNAAGGINKTFSPGDIVVDVDFINFTFKNPLRGPNLDEFGPRFPSLVEPVDK
NWVKSVIEKCKKDNIELKEGTYLWTLGPSYETPSEIRMFDKYEADLVGMSTLPEVMAANHVGLKVISFSAVTNMAAGILP
QPLKHEDVLRITEKIKGKFEKVVYNAIKLF
>Mature_269_residues
TTKIEKASQYIKESTEIKPILGLILGSGLGYIADQVENPKVIEYKDIPFFPQSTVVGHEGSLVIGTIEGIPVMILKGRFH
AYEGIELKDIVFPIYVMKDLGVKGLIITNAAGGINKTFSPGDIVVDVDFINFTFKNPLRGPNLDEFGPRFPSLVEPVDKN
WVKSVIEKCKKDNIELKEGTYLWTLGPSYETPSEIRMFDKYEADLVGMSTLPEVMAANHVGLKVISFSAVTNMAAGILPQ
PLKHEDVLRITEKIKGKFEKVVYNAIKLF

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=266, Percent_Identity=41.3533834586466, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI47132622, Length=223, Percent_Identity=27.3542600896861, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=40.0793650793651, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17541190, Length=252, Percent_Identity=38.0952380952381, Blast_Score=177, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6323238, Length=250, Percent_Identity=42, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24656093, Length=237, Percent_Identity=45.9915611814346, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24656090, Length=237, Percent_Identity=45.9915611814346, Blast_Score=210, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45552885, Length=237, Percent_Identity=45.9915611814346, Blast_Score=210, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45552887, Length=237, Percent_Identity=45.9915611814346, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24762376, Length=238, Percent_Identity=36.1344537815126, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI221459247, Length=286, Percent_Identity=28.3216783216783, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130079, Length=275, Percent_Identity=25.8181818181818, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29979; Mature: 29848

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKIEKASQYIKESTEIKPILGLILGSGLGYIADQVENPKVIEYKDIPFFPQSTVVGHE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCCCEECCC
GSLVIGTIEGIPVMILKGRFHAYEGIELKDIVFPIYVMKDLGVKGLIITNAAGGINKTFS
CCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PGDIVVDVDFINFTFKNPLRGPNLDEFGPRFPSLVEPVDKNWVKSVIEKCKKDNIELKEG
CCCEEEEEEEEEEEECCCCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEECC
TYLWTLGPSYETPSEIRMFDKYEADLVGMSTLPEVMAANHVGLKVISFSAVTNMAAGILP
EEEEEECCCCCCHHHHHHHHHHCCCEECHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCC
QPLKHEDVLRITEKIKGKFEKVVYNAIKLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKIEKASQYIKESTEIKPILGLILGSGLGYIADQVENPKVIEYKDIPFFPQSTVVGHE
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCCCEECCC
GSLVIGTIEGIPVMILKGRFHAYEGIELKDIVFPIYVMKDLGVKGLIITNAAGGINKTFS
CCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PGDIVVDVDFINFTFKNPLRGPNLDEFGPRFPSLVEPVDKNWVKSVIEKCKKDNIELKEG
CCCEEEEEEEEEEEECCCCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEECC
TYLWTLGPSYETPSEIRMFDKYEADLVGMSTLPEVMAANHVGLKVISFSAVTNMAAGILP
EEEEEECCCCCCHHHHHHHHHHCCCEECHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCC
QPLKHEDVLRITEKIKGKFEKVVYNAIKLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]