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The map label for this gene is pnp

Identifier: 160902045

GI number: 160902045

Start: 619293

End: 621380

Strand: Direct

Name: pnp

Synonym: Pmob_0569

Alternate gene names: 160902045

Gene position: 619293-621380 (Clockwise)

Preceding gene: 160902044

Following gene: 160902046

Centisome position: 28.54

GC content: 35.49

Gene sequence:

>2088_bases
ATGAAAGTATTGGAAAAAGAACTTTTTGGCAGAAAATTACGTATTGAACATGGTAAAGTTGCCAAACAGTCTCTTGGTTC
TGTGATGCTGACTTTTAACGAGTCAACTATACTAGTTACAGCAGACGCTTCGGAAGAAACAGTAAAAGGTCAGGACTTCT
TTCCATTAACCGTAGAATTTCAAGAAAAATTCTATGCAGTTGGTAAAATTCCAGGAGGTTTTATAAAAAGGGAAGGGAAA
CCAAGCGATGAAGCCATATTAGCTGCAAGGCTTATCGATAGACCTATTAGACCTTTGTTTCCAGAGAATTTTTTCAATGA
GGTCCAAGTAATAACTACGGTATTTTCAATGTTGAATGGCGATAGTATAGAAACATGGGGTATAACAGGTGCTTCGTTAG
CCCTGAATCTTTCACCTATACCATTTAATGGTATTGTTGCGGGAGTAAGGATAGGATACGTGGATGGACAATTCATTGCC
TTCCCAACCCAAGAACAACTAAAGAATTCTAAAATTGACATGGTCGTTGCAGGAACCAAAGATGCAGTTACAATGGTAGA
AGGTGAATCACTGGAAGTTTCTGAAGAAGAAATGGTAGAGGCTCTGATGTTCGCACAGGAAAAGATAAAAGAAATTATAG
CAATTGAAGAAGAGTTTCTTTCAGAATTAAACATTGAAAAATGGGAAGTTCAAAAAGAAATAGTTCCAGAGGAATTTATT
GAAGATTACCTATCTCTCATAGACGAAGAAGAGTTAAAAAAGGTACTACTCACAAAAGGTAAAAAAAACAGGGATAAAGT
TATATCAGAGTATAAAAAGAACGTAATGAAAAAATTTGAAGAAATCTTCTTGGAAAAATGGAGTGTGGCTTTTTTTGAAG
ATAAAAAACGTTTCTTAGAAAATGCTTTTGAAGAAAAACTTCAAGATTCGATGAGAAAAATGATTATTAACGAGAACAAA
AGAGTTGATGGAAGAACTTGTGATGAAATTAGAGATATCACCTGTGAAGTAGGGCTGATACCTAGAGTGCACGGTTCTGC
ACTTTTTACCAGAGGGGAAACTCAATCTCTAGGAACTGTTACTTTAGGTGCTCCTTTGGATGTTCAAGTTCTTGATACTA
TATTCAGCGACGAAGAAAAGAGGTTTATGCTCCATTATAATTTTCCTCCTTTCTCAACCGGTGAAGTAAAAAGGCTCAGA
GGAGTCAGCAGAAGAGAAATTGGCCATGGGCATTTAGCAGAAAGAGCACATAAAAACCTTATCCCAAGCGATGAAGAGTT
CCCTTATACTATTAGAGTCGTTTCTGATATTTTAGAATCAAATGGTTCCTCATCTATGGCTAGCGTATGCTCTGCCTCCT
TGGCTTTAATGGATGCAGGTGTACCAATCCAAAAACATGTTGCGGGTATTGCCATGGGTTTGATCTTTGAAAACGATAAT
TTCGTAGTTTTAACAGATATTTTAGGAATGGAGGACCACCTAGGAGACATGGATTTTAAAGTAGCAGGAACAAGAGATGG
AATAACTGCTTTTCAAATGGATGTTAAAACCAGTCAGGTTAATAAAGAAGTTCTTCAAAAGGCTTTAGAAAAAGCAAAAA
TAGCACGGTTGAAAATTTTAGATAAGATGTATGAAACTATCCCTCAACCACGCAAAGAATTGTCTCCATACGTTCCAATA
ATGAAAGTATTTAAAATACCCGTCTCAAAAATAGGAGAAGTAATCGGACCCGGTGGAAAAAATATTAAAGAGATCAGCGA
ATTATACAATGTTGAAGTTTATATAGAAGATGATGGAAAAGTCAAGGTAACTGGCCATAATGCAAACAAGGTTGATGAAG
CTATTAATCACATTCAAAATTTAATCGCACAAGTAGAAAAAGGTGGAATTTTTGAAGGTACAGTGAAAAGAGTCGAAAAA
TACGGTATATTTGTAGAAGTGTTACCCGGTAAGGTTGGAATGTTACATGTATCCAATTTGAAAGACAAACTTGAATCCTT
TAAAATTGGTGATAAGGTTAAAGTCGAAGTAATGAAGGTTGAAGACCAAGGAAAATTCCAATTAAAACAATTAAAAGAAG
AAAATTAA

Upstream 100 bases:

>100_bases
TTATCAAGAAATCATCAATAAATTAGGTATAAGAGGTTGATAACAAATTTTTTAAAAAGCGGGGCATAAGCTCCGTTTTG
TTTTTATAGGAGGTGTGAGA

Downstream 100 bases:

>100_bases
AAAATCAAACGTGCGGCTTTTTAACCGCATGTTTTTATTAATATTAGTAATAGATATTGTTTTTAGAAGTTCTAAAATTT
ATATAAAGCAACCCTTTCCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MKVLEKELFGRKLRIEHGKVAKQSLGSVMLTFNESTILVTADASEETVKGQDFFPLTVEFQEKFYAVGKIPGGFIKREGK
PSDEAILAARLIDRPIRPLFPENFFNEVQVITTVFSMLNGDSIETWGITGASLALNLSPIPFNGIVAGVRIGYVDGQFIA
FPTQEQLKNSKIDMVVAGTKDAVTMVEGESLEVSEEEMVEALMFAQEKIKEIIAIEEEFLSELNIEKWEVQKEIVPEEFI
EDYLSLIDEEELKKVLLTKGKKNRDKVISEYKKNVMKKFEEIFLEKWSVAFFEDKKRFLENAFEEKLQDSMRKMIINENK
RVDGRTCDEIRDITCEVGLIPRVHGSALFTRGETQSLGTVTLGAPLDVQVLDTIFSDEEKRFMLHYNFPPFSTGEVKRLR
GVSRREIGHGHLAERAHKNLIPSDEEFPYTIRVVSDILESNGSSSMASVCSASLALMDAGVPIQKHVAGIAMGLIFENDN
FVVLTDILGMEDHLGDMDFKVAGTRDGITAFQMDVKTSQVNKEVLQKALEKAKIARLKILDKMYETIPQPRKELSPYVPI
MKVFKIPVSKIGEVIGPGGKNIKEISELYNVEVYIEDDGKVKVTGHNANKVDEAINHIQNLIAQVEKGGIFEGTVKRVEK
YGIFVEVLPGKVGMLHVSNLKDKLESFKIGDKVKVEVMKVEDQGKFQLKQLKEEN

Sequences:

>Translated_695_residues
MKVLEKELFGRKLRIEHGKVAKQSLGSVMLTFNESTILVTADASEETVKGQDFFPLTVEFQEKFYAVGKIPGGFIKREGK
PSDEAILAARLIDRPIRPLFPENFFNEVQVITTVFSMLNGDSIETWGITGASLALNLSPIPFNGIVAGVRIGYVDGQFIA
FPTQEQLKNSKIDMVVAGTKDAVTMVEGESLEVSEEEMVEALMFAQEKIKEIIAIEEEFLSELNIEKWEVQKEIVPEEFI
EDYLSLIDEEELKKVLLTKGKKNRDKVISEYKKNVMKKFEEIFLEKWSVAFFEDKKRFLENAFEEKLQDSMRKMIINENK
RVDGRTCDEIRDITCEVGLIPRVHGSALFTRGETQSLGTVTLGAPLDVQVLDTIFSDEEKRFMLHYNFPPFSTGEVKRLR
GVSRREIGHGHLAERAHKNLIPSDEEFPYTIRVVSDILESNGSSSMASVCSASLALMDAGVPIQKHVAGIAMGLIFENDN
FVVLTDILGMEDHLGDMDFKVAGTRDGITAFQMDVKTSQVNKEVLQKALEKAKIARLKILDKMYETIPQPRKELSPYVPI
MKVFKIPVSKIGEVIGPGGKNIKEISELYNVEVYIEDDGKVKVTGHNANKVDEAINHIQNLIAQVEKGGIFEGTVKRVEK
YGIFVEVLPGKVGMLHVSNLKDKLESFKIGDKVKVEVMKVEDQGKFQLKQLKEEN
>Mature_695_residues
MKVLEKELFGRKLRIEHGKVAKQSLGSVMLTFNESTILVTADASEETVKGQDFFPLTVEFQEKFYAVGKIPGGFIKREGK
PSDEAILAARLIDRPIRPLFPENFFNEVQVITTVFSMLNGDSIETWGITGASLALNLSPIPFNGIVAGVRIGYVDGQFIA
FPTQEQLKNSKIDMVVAGTKDAVTMVEGESLEVSEEEMVEALMFAQEKIKEIIAIEEEFLSELNIEKWEVQKEIVPEEFI
EDYLSLIDEEELKKVLLTKGKKNRDKVISEYKKNVMKKFEEIFLEKWSVAFFEDKKRFLENAFEEKLQDSMRKMIINENK
RVDGRTCDEIRDITCEVGLIPRVHGSALFTRGETQSLGTVTLGAPLDVQVLDTIFSDEEKRFMLHYNFPPFSTGEVKRLR
GVSRREIGHGHLAERAHKNLIPSDEEFPYTIRVVSDILESNGSSSMASVCSASLALMDAGVPIQKHVAGIAMGLIFENDN
FVVLTDILGMEDHLGDMDFKVAGTRDGITAFQMDVKTSQVNKEVLQKALEKAKIARLKILDKMYETIPQPRKELSPYVPI
MKVFKIPVSKIGEVIGPGGKNIKEISELYNVEVYIEDDGKVKVTGHNANKVDEAINHIQNLIAQVEKGGIFEGTVKRVEK
YGIFVEVLPGKVGMLHVSNLKDKLESFKIGDKVKVEVMKVEDQGKFQLKQLKEEN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=678, Percent_Identity=36.1356932153392, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=45.095168374817, Blast_Score=587, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI115534063, Length=690, Percent_Identity=34.2028985507246, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI281362905, Length=678, Percent_Identity=36.283185840708, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651641, Length=678, Percent_Identity=36.283185840708, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651643, Length=678, Percent_Identity=36.283185840708, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI161079377, Length=623, Percent_Identity=36.7576243980738, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PETMO (A9BH73)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001567626.1
- ProteinModelPortal:   A9BH73
- SMR:   A9BH73
- GeneID:   5757941
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_0569
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   PMOB403833:PMOB_0569-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78199; Mature: 78199

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLEKELFGRKLRIEHGKVAKQSLGSVMLTFNESTILVTADASEETVKGQDFFPLTVEF
CCCHHHHHCCCEEEECCCHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEEEE
QEKFYAVGKIPGGFIKREGKPSDEAILAARLIDRPIRPLFPENFFNEVQVITTVFSMLNG
CHHEEEECCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DSIETWGITGASLALNLSPIPFNGIVAGVRIGYVDGQFIAFPTQEQLKNSKIDMVVAGTK
CCEEECCCCCCEEEEECCCCCCCCEEEEEEEEEECCCEEECCCHHHHCCCCEEEEEECCC
DAVTMVEGESLEVSEEEMVEALMFAQEKIKEIIAIEEEFLSELNIEKWEVQKEIVPEEFI
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
EDYLSLIDEEELKKVLLTKGKKNRDKVISEYKKNVMKKFEEIFLEKWSVAFFEDKKRFLE
HHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAFEEKLQDSMRKMIINENKRVDGRTCDEIRDITCEVGLIPRVHGSALFTRGETQSLGTV
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEECCCCCCCCCEEEECCCCCCCCEE
TLGAPLDVQVLDTIFSDEEKRFMLHYNFPPFSTGEVKRLRGVSRREIGHGHLAERAHKNL
EECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHCCHHHHCCCCHHHHHHHHCC
IPSDEEFPYTIRVVSDILESNGSSSMASVCSASLALMDAGVPIQKHVAGIAMGLIFENDN
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCC
FVVLTDILGMEDHLGDMDFKVAGTRDGITAFQMDVKTSQVNKEVLQKALEKAKIARLKIL
EEEEEECCCCHHHCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
DKMYETIPQPRKELSPYVPIMKVFKIPVSKIGEVIGPGGKNIKEISELYNVEVYIEDDGK
HHHHHHCCCCHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHEEEEEEECCCE
VKVTGHNANKVDEAINHIQNLIAQVEKGGIFEGTVKRVEKYGIFVEVLPGKVGMLHVSNL
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCEEEEEHHHH
KDKLESFKIGDKVKVEVMKVEDQGKFQLKQLKEEN
HHHHHHCCCCCEEEEEEEEECCCCCHHHHHHCCCC
>Mature Secondary Structure
MKVLEKELFGRKLRIEHGKVAKQSLGSVMLTFNESTILVTADASEETVKGQDFFPLTVEF
CCCHHHHHCCCEEEECCCHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEEEE
QEKFYAVGKIPGGFIKREGKPSDEAILAARLIDRPIRPLFPENFFNEVQVITTVFSMLNG
CHHEEEECCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DSIETWGITGASLALNLSPIPFNGIVAGVRIGYVDGQFIAFPTQEQLKNSKIDMVVAGTK
CCEEECCCCCCEEEEECCCCCCCCEEEEEEEEEECCCEEECCCHHHHCCCCEEEEEECCC
DAVTMVEGESLEVSEEEMVEALMFAQEKIKEIIAIEEEFLSELNIEKWEVQKEIVPEEFI
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
EDYLSLIDEEELKKVLLTKGKKNRDKVISEYKKNVMKKFEEIFLEKWSVAFFEDKKRFLE
HHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAFEEKLQDSMRKMIINENKRVDGRTCDEIRDITCEVGLIPRVHGSALFTRGETQSLGTV
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEECCCCCCCCCEEEECCCCCCCCEE
TLGAPLDVQVLDTIFSDEEKRFMLHYNFPPFSTGEVKRLRGVSRREIGHGHLAERAHKNL
EECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHCCHHHHCCCCHHHHHHHHCC
IPSDEEFPYTIRVVSDILESNGSSSMASVCSASLALMDAGVPIQKHVAGIAMGLIFENDN
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCC
FVVLTDILGMEDHLGDMDFKVAGTRDGITAFQMDVKTSQVNKEVLQKALEKAKIARLKIL
EEEEEECCCCHHHCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
DKMYETIPQPRKELSPYVPIMKVFKIPVSKIGEVIGPGGKNIKEISELYNVEVYIEDDGK
HHHHHHCCCCHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHEEEEEEECCCE
VKVTGHNANKVDEAINHIQNLIAQVEKGGIFEGTVKRVEKYGIFVEVLPGKVGMLHVSNL
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCEEEEEHHHH
KDKLESFKIGDKVKVEVMKVEDQGKFQLKQLKEEN
HHHHHHCCCCCEEEEEEEEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA