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The map label for this gene is ahpF [H]

Identifier: 160902042

GI number: 160902042

Start: 617142

End: 617813

Strand: Direct

Name: ahpF [H]

Synonym: Pmob_0566

Alternate gene names: 160902042

Gene position: 617142-617813 (Clockwise)

Preceding gene: 160902041

Following gene: 160902043

Centisome position: 28.45

GC content: 34.67

Gene sequence:

>672_bases
ATGGAAAAATTGATGGACAAAGAAACACAAAACAAGGTAAGAGAAATTTTAGAGGAATTGACAGAGCCTGTTCAAATATT
TTTATTCAAAAATGATGGAGAATATTCAGAAATTGTTGAACAGTTACTTGGAGAGCTCAAGGAATTAGATGATAGAATAA
AAGTTGATACCTACCATTCTGATTCTGAAGAAATTAACAACTACGATATTGAAAGAGATTTATTTCCAGCTATGGTAATA
TTAGATAGCGAAGGAAACGATTACGGAATTAGATATTATGGGATCCCTTCGGGATATGAGTTCACTACTCTGCTTCAAAA
TCTCATCGCTGTATCTAATAATAGTGTGACCTCTTTCAGCGATGAAAACAAAGAAAAATTATCTAAAATAGACAAAAAGA
TGAGGATCAGAGTTTTCGTGACTCCAACTTGTCCATACTGCCCTAGGGCTGTTTTTGCAGCTCATCAAGCCGCTATGTTA
AACCCTAATATAACAGGAGAAATGATAGAAGCTAACGAATTCGATCGAATATCTTTTGAATATGGTGTAAGTTCCGTTCC
ACATACCGTGATAGAAGTAAAAGAAAGCGAAGAATGGGTAAAAAAAGGTGAGTTTGTGGGAGCCTACCCTGAAAATAGTT
TTGTGGAAGAAGTTTTAAAAGCAGTTGAATAA

Upstream 100 bases:

>100_bases
TTAACAAAACATATAGAATTTTAAAGTAGATTAAATCAAAATATGTTAATATACCTATATAGGGTATATACAACACTAAA
TTAGATGGAGGTTTTTAAAT

Downstream 100 bases:

>100_bases
AGTAGTTGAAGGAGGTTCTCAGATGTTTTTTAACGTTGAAGGGGCTAACAAAAAATCCGAAATAAAACAACAATATGACG
TTGTAATAATCGGTGGCGGT

Product: glutaredoxin-like domain-containing protein

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MEKLMDKETQNKVREILEELTEPVQIFLFKNDGEYSEIVEQLLGELKELDDRIKVDTYHSDSEEINNYDIERDLFPAMVI
LDSEGNDYGIRYYGIPSGYEFTTLLQNLIAVSNNSVTSFSDENKEKLSKIDKKMRIRVFVTPTCPYCPRAVFAAHQAAML
NPNITGEMIEANEFDRISFEYGVSSVPHTVIEVKESEEWVKKGEFVGAYPENSFVEEVLKAVE

Sequences:

>Translated_223_residues
MEKLMDKETQNKVREILEELTEPVQIFLFKNDGEYSEIVEQLLGELKELDDRIKVDTYHSDSEEINNYDIERDLFPAMVI
LDSEGNDYGIRYYGIPSGYEFTTLLQNLIAVSNNSVTSFSDENKEKLSKIDKKMRIRVFVTPTCPYCPRAVFAAHQAAML
NPNITGEMIEANEFDRISFEYGVSSVPHTVIEVKESEEWVKKGEFVGAYPENSFVEEVLKAVE
>Mature_223_residues
MEKLMDKETQNKVREILEELTEPVQIFLFKNDGEYSEIVEQLLGELKELDDRIKVDTYHSDSEEINNYDIERDLFPAMVI
LDSEGNDYGIRYYGIPSGYEFTTLLQNLIAVSNNSVTSFSDENKEKLSKIDKKMRIRVFVTPTCPYCPRAVFAAHQAAML
NPNITGEMIEANEFDRISFEYGVSSVPHTVIEVKESEEWVKKGEFVGAYPENSFVEEVLKAVE

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=192, Percent_Identity=25.5208333333333, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 25632; Mature: 25632

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLMDKETQNKVREILEELTEPVQIFLFKNDGEYSEIVEQLLGELKELDDRIKVDTYHS
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
DSEEINNYDIERDLFPAMVILDSEGNDYGIRYYGIPSGYEFTTLLQNLIAVSNNSVTSFS
CHHHHCCCCCCHHCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
DENKEKLSKIDKKMRIRVFVTPTCPYCPRAVFAAHQAAMLNPNITGEMIEANEFDRISFE
CCCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEHH
YGVSSVPHTVIEVKESEEWVKKGEFVGAYPENSFVEEVLKAVE
CCCCCCCHHHEEEHHHHHHHHCCCEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEKLMDKETQNKVREILEELTEPVQIFLFKNDGEYSEIVEQLLGELKELDDRIKVDTYHS
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
DSEEINNYDIERDLFPAMVILDSEGNDYGIRYYGIPSGYEFTTLLQNLIAVSNNSVTSFS
CHHHHCCCCCCHHCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
DENKEKLSKIDKKMRIRVFVTPTCPYCPRAVFAAHQAAMLNPNITGEMIEANEFDRISFE
CCCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEHH
YGVSSVPHTVIEVKESEEWVKKGEFVGAYPENSFVEEVLKAVE
CCCCCCCHHHEEEHHHHHHHHCCCEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]