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The map label for this gene is def [H]

Identifier: 160901540

GI number: 160901540

Start: 44757

End: 45293

Strand: Direct

Name: def [H]

Synonym: Pmob_0049

Alternate gene names: 160901540

Gene position: 44757-45293 (Clockwise)

Preceding gene: 160901539

Following gene: 160901541

Centisome position: 2.06

GC content: 36.87

Gene sequence:

>537_bases
ATGGAAATTAGACTTATTGGGGACCCTGTTTTAAGAAAAAGGGCAAAAAAAGTGGAAAATTTCGATGACAATTTAAAAGA
TGTCGTTGATGAGATGTTTTCTACAATGTATCTATACGATGGAGTAGGTTTAGCTGCACCTCAAGTGGGAATATCTTTGA
GGTTTTTCATCATGGATTCTCGGGAAGAAAATGAAAAGGGCAAAAAAGTTGTTATTAATCCCGAAATAATAGAGTTCTTA
GGAGAAGAGGTTAGCTTTGAAGAAGGATGCCTAAGCATTCCTGATATATTTGAAGATGTTGTTAGACCTGAAGGTGTGAG
GGTGCGTTATCAGGATTTAAGTGGCAACGTTATAGAGGAAGAACTTCACGGGTATCAGGCAAGAATATTTCAACATGAAA
CAGATCACCTTGAGGGAATTCTATTCACTGACAAATTACCCATTGTAAAGAAAGCCAGGTTAAAAAAGGAATTGAACCAA
CTAATAGAAAAGGGCAAAAAACGTGCATGGGAGTTAGGTGAAACGGTAAAAACATGA

Upstream 100 bases:

>100_bases
GTAATAAATCAAGGTTATGTTGCTGTAACGCCGCTAAGTGTTTTCATGACAAAATATGATTTTATAGATGAATTAAAATC
TTGGTTGGAGGTTTAAAAAG

Downstream 100 bases:

>100_bases
CAAATAGTAAGGATTTTAAAATTGTTTTCATGGGGACACCTGATTTTGGTGCCCAAGTTTTGGAAGAACTAATAAAAAAC
AATTTCAATGTTGTTGGAGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MEIRLIGDPVLRKRAKKVENFDDNLKDVVDEMFSTMYLYDGVGLAAPQVGISLRFFIMDSREENEKGKKVVINPEIIEFL
GEEVSFEEGCLSIPDIFEDVVRPEGVRVRYQDLSGNVIEEELHGYQARIFQHETDHLEGILFTDKLPIVKKARLKKELNQ
LIEKGKKRAWELGETVKT

Sequences:

>Translated_178_residues
MEIRLIGDPVLRKRAKKVENFDDNLKDVVDEMFSTMYLYDGVGLAAPQVGISLRFFIMDSREENEKGKKVVINPEIIEFL
GEEVSFEEGCLSIPDIFEDVVRPEGVRVRYQDLSGNVIEEELHGYQARIFQHETDHLEGILFTDKLPIVKKARLKKELNQ
LIEKGKKRAWELGETVKT
>Mature_178_residues
MEIRLIGDPVLRKRAKKVENFDDNLKDVVDEMFSTMYLYDGVGLAAPQVGISLRFFIMDSREENEKGKKVVINPEIIEFL
GEEVSFEEGCLSIPDIFEDVVRPEGVRVRYQDLSGNVIEEELHGYQARIFQHETDHLEGILFTDKLPIVKKARLKKELNQ
LIEKGKKRAWELGETVKT

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=30.8176100628931, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=42.603550295858, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645728, Length=160, Percent_Identity=39.375, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=36.4779874213836, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20539; Mature: 20539

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRLIGDPVLRKRAKKVENFDDNLKDVVDEMFSTMYLYDGVGLAAPQVGISLRFFIMDS
CEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
REENEKGKKVVINPEIIEFLGEEVSFEEGCLSIPDIFEDVVRPEGVRVRYQDLSGNVIEE
CCCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHH
ELHGYQARIFQHETDHLEGILFTDKLPIVKKARLKKELNQLIEKGKKRAWELGETVKT
HHHHHHHHHHHCCCHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIRLIGDPVLRKRAKKVENFDDNLKDVVDEMFSTMYLYDGVGLAAPQVGISLRFFIMDS
CEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
REENEKGKKVVINPEIIEFLGEEVSFEEGCLSIPDIFEDVVRPEGVRVRYQDLSGNVIEE
CCCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHH
ELHGYQARIFQHETDHLEGILFTDKLPIVKKARLKKELNQLIEKGKKRAWELGETVKT
HHHHHHHHHHHCCCHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9086272; 10360571 [H]