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The map label for this gene is mutM

Identifier: 160877565

GI number: 160877565

Start: 5274128

End: 5274943

Strand: Direct

Name: mutM

Synonym: Sbal195_4464

Alternate gene names: 160877565

Gene position: 5274128-5274943 (Clockwise)

Preceding gene: 160877564

Following gene: 160877569

Centisome position: 98.63

GC content: 51.47

Gene sequence:

>816_bases
ATGCCGGAATTACCAGAAGTTGAAGTCACCCGTCAGGGTATTGCACCCTTCCTTGTCGAGCAAACCGTGGTTGATCTTGT
GATCCGCAATGGTTCGCTGCGCTGGCCAGTGCCTGACATCGCCAAACAAATTATCGGCCAAGTCATACGCCAAGTGCGTC
GCCGTGCTAAGTATCTGTTAATCGACACTGATGCGGGCACTAGCATAGTGCATTTAGGCATGTCGGGGAGTTTACGGATT
TTGCCCCACGATACCCCAGTGGAAAAGCATGACCATATAGACTTAGTGCTCGCCAACGGCCGCATTTTACGCTTTAACGA
TCCTAGACGTTTCGGTGCTTGGCTATGGTGTGAACTCCCAGAAGAAGCGCATCCATTACTCGCCAAACTGGGGCCAGAGC
CGCTGACCAATGCTTTTAATGTCACACAATTAGCGGCAGCTTTAGCGGGCAAGAAAAAGGCCATCAAACTCTGCCTGATG
GATAATCATATCGTGGTGGGAGTGGGCAATATTTACGCCAATGAAGCCCTGTTTGCGGCGGGCATTCACCCAGAAGCCGA
AGCAGGTAAAATCGATATCGAGCGCTTGACCGTTTTAGTTGCCGAAGTGAAGCAAATCTTAGCCCACGCCATTAAGCAAG
GCGGCACTACGTTGAAAGACTTTACCAATGCCGATGGCAAGCCGGGCTACTTCGCCCAAAAGCTGCATGTCTATAGTCGC
GGCGGTGAAACCTGCACCAGCTGCGGTAACCTACTGAGTGAGATCCGTTTAGGCCAACGCACCACAGTATTCTGTGGCAT
TTGCCAGACACGCTAA

Upstream 100 bases:

>100_bases
ATTTAGTGCCTCCGAGTTTACGAGCTAAACTGCAACCGATCCTCGATGCGAAACCCGCAGCTTAATGCCGCCCCATTTTA
CTTAAGATAAGAAAGAATCC

Downstream 100 bases:

>100_bases
CTATCACTCGCTAATAGCCGTAAAAATGCCTGCAATTGCAGGCATTTTATTCTTTATATTGGTTACTCTTTTAACCAGCG
CCCCGGATGTTCGGTAAAGT

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPELPEVEVTRQGIAPFLVEQTVVDLVIRNGSLRWPVPDIAKQIIGQVIRQVRRRAKYLLIDTDAGTSIVHLGMSGSLRI
LPHDTPVEKHDHIDLVLANGRILRFNDPRRFGAWLWCELPEEAHPLLAKLGPEPLTNAFNVTQLAAALAGKKKAIKLCLM
DNHIVVGVGNIYANEALFAAGIHPEAEAGKIDIERLTVLVAEVKQILAHAIKQGGTTLKDFTNADGKPGYFAQKLHVYSR
GGETCTSCGNLLSEIRLGQRTTVFCGICQTR

Sequences:

>Translated_271_residues
MPELPEVEVTRQGIAPFLVEQTVVDLVIRNGSLRWPVPDIAKQIIGQVIRQVRRRAKYLLIDTDAGTSIVHLGMSGSLRI
LPHDTPVEKHDHIDLVLANGRILRFNDPRRFGAWLWCELPEEAHPLLAKLGPEPLTNAFNVTQLAAALAGKKKAIKLCLM
DNHIVVGVGNIYANEALFAAGIHPEAEAGKIDIERLTVLVAEVKQILAHAIKQGGTTLKDFTNADGKPGYFAQKLHVYSR
GGETCTSCGNLLSEIRLGQRTTVFCGICQTR
>Mature_270_residues
PELPEVEVTRQGIAPFLVEQTVVDLVIRNGSLRWPVPDIAKQIIGQVIRQVRRRAKYLLIDTDAGTSIVHLGMSGSLRIL
PHDTPVEKHDHIDLVLANGRILRFNDPRRFGAWLWCELPEEAHPLLAKLGPEPLTNAFNVTQLAAALAGKKKAIKLCLMD
NHIVVGVGNIYANEALFAAGIHPEAEAGKIDIERLTVLVAEVKQILAHAIKQGGTTLKDFTNADGKPGYFAQKLHVYSRG
GETCTSCGNLLSEIRLGQRTTVFCGICQTR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=270, Percent_Identity=55.5555555555556, Blast_Score=305, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_SHEB2 (B8EDQ9)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002360158.1
- ProteinModelPortal:   B8EDQ9
- SMR:   B8EDQ9
- GeneID:   7089210
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_4269
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 29743; Mature: 29612

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 57-57 ACT_SITE 260-260 BINDING 90-90 BINDING 109-109 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVTRQGIAPFLVEQTVVDLVIRNGSLRWPVPDIAKQIIGQVIRQVRRRAKYLL
CCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
IDTDAGTSIVHLGMSGSLRILPHDTPVEKHDHIDLVLANGRILRFNDPRRFGAWLWCELP
EECCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCEEEEECCCCHHCCEEEEECC
EEAHPLLAKLGPEPLTNAFNVTQLAAALAGKKKAIKLCLMDNHIVVGVGNIYANEALFAA
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCHHCCEEEEE
GIHPEAEAGKIDIERLTVLVAEVKQILAHAIKQGGTTLKDFTNADGKPGYFAQKLHVYSR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCCHHHHHEEEECC
GGETCTSCGNLLSEIRLGQRTTVFCGICQTR
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure 
PELPEVEVTRQGIAPFLVEQTVVDLVIRNGSLRWPVPDIAKQIIGQVIRQVRRRAKYLL
CCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
IDTDAGTSIVHLGMSGSLRILPHDTPVEKHDHIDLVLANGRILRFNDPRRFGAWLWCELP
EECCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCEEEEECCCCHHCCEEEEECC
EEAHPLLAKLGPEPLTNAFNVTQLAAALAGKKKAIKLCLMDNHIVVGVGNIYANEALFAA
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCHHCCEEEEE
GIHPEAEAGKIDIERLTVLVAEVKQILAHAIKQGGTTLKDFTNADGKPGYFAQKLHVYSR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCCHHHHHEEEECC
GGETCTSCGNLLSEIRLGQRTTVFCGICQTR
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA