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The map label for this gene is menF [H]

Identifier: 160877552

GI number: 160877552

Start: 5260161

End: 5261519

Strand: Direct

Name: menF [H]

Synonym: Sbal195_4451

Alternate gene names: 160877552

Gene position: 5260161-5261519 (Clockwise)

Preceding gene: 160877543

Following gene: 160877554

Centisome position: 98.37

GC content: 48.2

Gene sequence:

>1359_bases
TTGCCCGCTCATGCCCTGTCTGAAGACCTTAAGTCTCTCATCGACAAACTCATTCAATTAAAGCAAGTTCCGCCGACGGA
ACCGATAGCGCAGTTGTCGCTTACAACTGTCGCTATGCCTTTAATTTCATGGTTGGCATCGCAGACACAATTTCCACGTA
TTTACTGGCATGGGCGTGACAGAATCGAAGAAGTCGCGGCGATTGGGGTATGTAAAGACTTCAAATTTGAAACCGGAATC
AATGATCAGCAACTGGCCATGGCCTACCAAGAGCAGCGAGCCTTATCGACGAATCAGAATATTCGCTACTATGGCGGTGT
TGCCTTCGATCGCACTATAGAGTCTTGGCCTGAGTTCGGTAATAGCCGCTTTATCCTGCCGCGCATTGAATTTAGACGCA
GCGGTAGCAAGTTTAGTCTGTTAGTAAATCTTAATTTTGCCGAGAATGATCACCATAGCGAAATCGACAAGGCCATCGCC
GCCATCGAAGCTGTGATGCCCGCTAAACCACTCTCACCACCGAAGAAAATCGTTCCTCTTGGCCGCAGTGACAAGCCCAA
TTTTGAACGCTGGAAAACCTTAGTCGAGCAAGTGGTGGATCCTAAGTTCAATCAAGATACCCCAAAAGTCGTGTTATCCC
GCTTAACGCAACTTGAAGTCAACGAACCTATCGATCCTTGGATGGTGCTGGCTTGCTGGCAGGGACGTAATCCCAACAGC
TTCCAATTTGGCTTCCAATTCAGTCCAGAACGCACCTTTATCTCTTGCTCACCAGAGCGTTTATTCCGCCGCCGTCAGCA
GGAATTATTCACTGAGGCACTCGCAGGTACGACAGTTCGCGGCCTCAATCAAGAAGAAGATATTGCGCTGGCGAATGCCT
TGCTAGAAGACAACAAAAATAGCGTTGAGAACCAACTCGTTCGCAGTCACATAGTGAATATGCTCACACCGCTGAGTAAT
TATGTTGGCGCCGATGAATTAGCGACCATCTTTAAACTGAATCATATTCAACATTTACATCGCGCAATTCGAGCCGAATT
AAAACCGGGCGTGAATGATTTCCAACTGTTGCAAGCGCTGCATCCAACCCCTGCAGTGGGTGGCTTACCGCGGCATTCGG
CATTGAACTTTATTCGTCAGCGTGAAGGTTATATTCGTGGCTGGTACGCTGGTGCCTGCGGTTATTTCAATAAATACGAG
AGTGAGTTTTCAGTGGCAATCCGCAGTGCGTTGATCGAACCCGGTCGGATTAACCTATTTGCGGGGGCGGGAATTATTGC
GGGTTCAGAGCCCGAAGCTGAGTGGCAAGAGCTTGAAAATAAACTCGCGACTATCATGTCGATTCTGTTCGAAATGTAA

Upstream 100 bases:

>100_bases
ACTTGAAAAAGAGTACATTAACCTGAGTCGAATTTATTAACGCTGACAGAGTGACCTTCAGCTTTCGCCTTTATCCCTTA
TCTTTACTTTGGAGTTCTAT

Downstream 100 bases:

>100_bases
TAAACCGAATAAATTTAAGGCCTATTCATCATCAGCCTTTCTTTTTTCAAGTATTTCAAGATAAAGCCTGTCCTGCTCTC
CCGAATCGATTAAACGCTTA

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPAHALSEDLKSLIDKLIQLKQVPPTEPIAQLSLTTVAMPLISWLASQTQFPRIYWHGRDRIEEVAAIGVCKDFKFETGI
NDQQLAMAYQEQRALSTNQNIRYYGGVAFDRTIESWPEFGNSRFILPRIEFRRSGSKFSLLVNLNFAENDHHSEIDKAIA
AIEAVMPAKPLSPPKKIVPLGRSDKPNFERWKTLVEQVVDPKFNQDTPKVVLSRLTQLEVNEPIDPWMVLACWQGRNPNS
FQFGFQFSPERTFISCSPERLFRRRQQELFTEALAGTTVRGLNQEEDIALANALLEDNKNSVENQLVRSHIVNMLTPLSN
YVGADELATIFKLNHIQHLHRAIRAELKPGVNDFQLLQALHPTPAVGGLPRHSALNFIRQREGYIRGWYAGACGYFNKYE
SEFSVAIRSALIEPGRINLFAGAGIIAGSEPEAEWQELENKLATIMSILFEM

Sequences:

>Translated_452_residues
MPAHALSEDLKSLIDKLIQLKQVPPTEPIAQLSLTTVAMPLISWLASQTQFPRIYWHGRDRIEEVAAIGVCKDFKFETGI
NDQQLAMAYQEQRALSTNQNIRYYGGVAFDRTIESWPEFGNSRFILPRIEFRRSGSKFSLLVNLNFAENDHHSEIDKAIA
AIEAVMPAKPLSPPKKIVPLGRSDKPNFERWKTLVEQVVDPKFNQDTPKVVLSRLTQLEVNEPIDPWMVLACWQGRNPNS
FQFGFQFSPERTFISCSPERLFRRRQQELFTEALAGTTVRGLNQEEDIALANALLEDNKNSVENQLVRSHIVNMLTPLSN
YVGADELATIFKLNHIQHLHRAIRAELKPGVNDFQLLQALHPTPAVGGLPRHSALNFIRQREGYIRGWYAGACGYFNKYE
SEFSVAIRSALIEPGRINLFAGAGIIAGSEPEAEWQELENKLATIMSILFEM
>Mature_451_residues
PAHALSEDLKSLIDKLIQLKQVPPTEPIAQLSLTTVAMPLISWLASQTQFPRIYWHGRDRIEEVAAIGVCKDFKFETGIN
DQQLAMAYQEQRALSTNQNIRYYGGVAFDRTIESWPEFGNSRFILPRIEFRRSGSKFSLLVNLNFAENDHHSEIDKAIAA
IEAVMPAKPLSPPKKIVPLGRSDKPNFERWKTLVEQVVDPKFNQDTPKVVLSRLTQLEVNEPIDPWMVLACWQGRNPNSF
QFGFQFSPERTFISCSPERLFRRRQQELFTEALAGTTVRGLNQEEDIALANALLEDNKNSVENQLVRSHIVNMLTPLSNY
VGADELATIFKLNHIQHLHRAIRAELKPGVNDFQLLQALHPTPAVGGLPRHSALNFIRQREGYIRGWYAGACGYFNKYES
EFSVAIRSALIEPGRINLFAGAGIIAGSEPEAEWQELENKLATIMSILFEM

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=450, Percent_Identity=34.4444444444444, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786809, Length=242, Percent_Identity=29.3388429752066, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787518, Length=253, Percent_Identity=28.8537549407115, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788114, Length=200, Percent_Identity=29, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=292, Percent_Identity=29.1095890410959, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324361, Length=273, Percent_Identity=24.1758241758242, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51225; Mature: 51094

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHALSEDLKSLIDKLIQLKQVPPTEPIAQLSLTTVAMPLISWLASQTQFPRIYWHGRD
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
RIEEVAAIGVCKDFKFETGINDQQLAMAYQEQRALSTNQNIRYYGGVAFDRTIESWPEFG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCC
NSRFILPRIEFRRSGSKFSLLVNLNFAENDHHSEIDKAIAAIEAVMPAKPLSPPKKIVPL
CCEEEEECHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEECC
GRSDKPNFERWKTLVEQVVDPKFNQDTPKVVLSRLTQLEVNEPIDPWMVLACWQGRNPNS
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
FQFGFQFSPERTFISCSPERLFRRRQQELFTEALAGTTVRGLNQEEDIALANALLEDNKN
EEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCH
SVENQLVRSHIVNMLTPLSNYVGADELATIFKLNHIQHLHRAIRAELKPGVNDFQLLQAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
HPTPAVGGLPRHSALNFIRQREGYIRGWYAGACGYFNKYESEFSVAIRSALIEPGRINLF
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGAGIIAGSEPEAEWQELENKLATIMSILFEM
ECCCEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAHALSEDLKSLIDKLIQLKQVPPTEPIAQLSLTTVAMPLISWLASQTQFPRIYWHGRD
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
RIEEVAAIGVCKDFKFETGINDQQLAMAYQEQRALSTNQNIRYYGGVAFDRTIESWPEFG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCC
NSRFILPRIEFRRSGSKFSLLVNLNFAENDHHSEIDKAIAAIEAVMPAKPLSPPKKIVPL
CCEEEEECHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEECC
GRSDKPNFERWKTLVEQVVDPKFNQDTPKVVLSRLTQLEVNEPIDPWMVLACWQGRNPNS
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
FQFGFQFSPERTFISCSPERLFRRRQQELFTEALAGTTVRGLNQEEDIALANALLEDNKN
EEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCH
SVENQLVRSHIVNMLTPLSNYVGADELATIFKLNHIQHLHRAIRAELKPGVNDFQLLQAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
HPTPAVGGLPRHSALNFIRQREGYIRGWYAGACGYFNKYESEFSVAIRSALIEPGRINLF
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGAGIIAGSEPEAEWQELENKLATIMSILFEM
ECCCEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]