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The map label for this gene is rsmD [H]

Identifier: 160877398

GI number: 160877398

Start: 5077521

End: 5078189

Strand: Direct

Name: rsmD [H]

Synonym: Sbal195_4296

Alternate gene names: 160877398

Gene position: 5077521-5078189 (Clockwise)

Preceding gene: 160877393

Following gene: 160877399

Centisome position: 94.96

GC content: 48.88

Gene sequence:

>669_bases
TTGGTTAAGAATCGAACGGTCAAAAATAATGTGACCAAAAGTCCAACCGCCAAAAATAAACCCGGTAGCGGTCAAGTGCG
GATCATCGCGGGCCAATGGCGTTCGCGTCGTTTACCTATTCAAGATCTCGAAGGTTTACGCCCGACAACAGACAGAGTGC
GCGAAACCCTGTTTAACTGGCTTGCCAATGACTTAGTTAATGCGCGAGTGCTCGACTGTTTCGGCGGCAGCGGCGCTTTA
GCCCTCGAAGCCCTATCTCGCTATGCCAGTTTCGCCAAGATTATCGAGTTGCAAAGCGGCGCAGCCATGCAATTAAAAGA
AAATCTGCAAACCCTAAAATGCGACAAGGCCGAAGTGCTCAACGCCGATACCTTAGTGGTGTTGCAAAGAGGCTGCGAGC
AAGGTTTCGATGTAGTGTTTATCGACCCTCCCTTTCGCAAGGGATTGGCAGAAAAAACCATTCAACTGCTCGATACTCAA
GCTTGGCTCAATGATGGCGCGCTGATTTATGTCGAGATCGAATCTGAATTAACCCAACTGGCCATTCCATCTAGCTGGCA
CGCCTTGAAAGAAAAGAATGCGGGGCAAGTGAGTTACCGCTTATATCAATATCATGCCGACAACACTGCCGAACCTATCG
AAGATGAAAGCCATGCCCTTGCTGATTAA

Upstream 100 bases:

>100_bases
CCTTCTCACCTTTTAGTGAGACTTTGATATAAAATAGTGGCCTTTATTAAGGTGGCATAGTCTACCACTTTCTTTCTTCA
GGCAAAATCACGGGGGCACT

Downstream 100 bases:

>100_bases
CCTAGGTAAAGCCATTACCCTCATCGCTTGGTTGATGATGGCCTACAACTTGGTTATGCCATTTGAAGGTAATGTGGCGA
TTATTTTGAATATCCTGTTT

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MVKNRTVKNNVTKSPTAKNKPGSGQVRIIAGQWRSRRLPIQDLEGLRPTTDRVRETLFNWLANDLVNARVLDCFGGSGAL
ALEALSRYASFAKIIELQSGAAMQLKENLQTLKCDKAEVLNADTLVVLQRGCEQGFDVVFIDPPFRKGLAEKTIQLLDTQ
AWLNDGALIYVEIESELTQLAIPSSWHALKEKNAGQVSYRLYQYHADNTAEPIEDESHALAD

Sequences:

>Translated_222_residues
MVKNRTVKNNVTKSPTAKNKPGSGQVRIIAGQWRSRRLPIQDLEGLRPTTDRVRETLFNWLANDLVNARVLDCFGGSGAL
ALEALSRYASFAKIIELQSGAAMQLKENLQTLKCDKAEVLNADTLVVLQRGCEQGFDVVFIDPPFRKGLAEKTIQLLDTQ
AWLNDGALIYVEIESELTQLAIPSSWHALKEKNAGQVSYRLYQYHADNTAEPIEDESHALAD
>Mature_222_residues
MVKNRTVKNNVTKSPTAKNKPGSGQVRIIAGQWRSRRLPIQDLEGLRPTTDRVRETLFNWLANDLVNARVLDCFGGSGAL
ALEALSRYASFAKIIELQSGAAMQLKENLQTLKCDKAEVLNADTLVVLQRGCEQGFDVVFIDPPFRKGLAEKTIQLLDTQ
AWLNDGALIYVEIESELTQLAIPSSWHALKEKNAGQVSYRLYQYHADNTAEPIEDESHALAD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=194, Percent_Identity=55.6701030927835, Blast_Score=217, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 24709; Mature: 24709

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNRTVKNNVTKSPTAKNKPGSGQVRIIAGQWRSRRLPIQDLEGLRPTTDRVRETLFNW
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHH
LANDLVNARVLDCFGGSGALALEALSRYASFAKIIELQSGAAMQLKENLQTLKCDKAEVL
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHC
NADTLVVLQRGCEQGFDVVFIDPPFRKGLAEKTIQLLDTQAWLNDGALIYVEIESELTQL
CCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
AIPSSWHALKEKNAGQVSYRLYQYHADNTAEPIEDESHALAD
CCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVKNRTVKNNVTKSPTAKNKPGSGQVRIIAGQWRSRRLPIQDLEGLRPTTDRVRETLFNW
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHH
LANDLVNARVLDCFGGSGALALEALSRYASFAKIIELQSGAAMQLKENLQTLKCDKAEVL
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHC
NADTLVVLQRGCEQGFDVVFIDPPFRKGLAEKTIQLLDTQAWLNDGALIYVEIESELTQL
CCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
AIPSSWHALKEKNAGQVSYRLYQYHADNTAEPIEDESHALAD
CCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]