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The map label for this gene is cpsA1 [H]

Identifier: 160877359

GI number: 160877359

Start: 5038391

End: 5039788

Strand: Reverse

Name: cpsA1 [H]

Synonym: Sbal195_4257

Alternate gene names: 160877359

Gene position: 5039788-5038391 (Counterclockwise)

Preceding gene: 160877360

Following gene: 160877358

Centisome position: 94.25

GC content: 51.22

Gene sequence:

>1398_bases
ATGACTTTACCCTCAGTATCACTTGCTGCATCAACATCATTTGAATTTAAGCCCCTAGTAAATAAATCCCATTTTTCTAA
GACCTTACTCGCTTCAGCCTTAGCCTTGAGCTTTATGGGCGCGGGTTTGAGTTCGGCGCAAGCGGCGACCAGTGCTATGC
CGGATGCGGCTAAGTTGGCGGCGGGTGTGGAGCAAAAGGTGATCGACTGGCGCCGCGATTTACATCAACACCCTGAGTTG
TCAAATCGTGAATTTCGCACCAGCAAGATTATTGAAAAGCATCTGAAATCATTAGGATTGGAAGTACAAACGGGTGTGGC
ACATACAGGCGTGGTGGCCATTTTGAAAGGCGGAAAGCTGAAAGGTGGCAAGTCTGGTCCGTTGATTGCGATTCGGGCGG
ATATGGATGCTTTACCTGTGACTGAGGTGGTCGATTTGCCCTTTGCCTCTAAAGCGACCGATACCTATCGTGATCAAAAA
GTGGGGGTGATGCATGCCTGTGGCCACGATACCCATGTGGCCATGTTGATGGGCGTGGCTGAAAACCTCGTGAAAGTGAA
AGACAGCCTCGCGGGTGATGTGATGTTTATCTTCCAACCTGCCGAAGAAGGCGCGCCAGACGGGGAAGAAGGCGGCGCGG
AGTTAATGCTTAAAGAAGGGTTGTTTGCTAAGCGTAAACCTGACCAAGTGTTTGGTATGCATGTTACCTCCAGCATGCCG
AGTGGCATGATTGGTGTGCGCAGTGGCCCGGCGATGGCGAGTGAAGATTCCTTTACGATCAAAGTGAAAGGTCGCCAAAC
CCATGGTTCGCGCCCTTGGAATGGGGTCGATCCTATTGTCGCTGCGGCGCAAATTATCACTAACGTGCAAACGATCGTCA
GCCGTCAGGTGGATATCACTAAAGCCCCTGCCGTGGTGAGCTTTGGTGCGGTTAATGGTGGCATTCGCTCTAACATTATT
CCCGATGAAGTCGAGCTGATTGGCACTATTCGCACCTTCGACCAGCCCATGCGCGCCGATATCAAAGTGCGTTTAGCTGA
AATGGCCGAGTTGTCGGCGAAAACGCTTGGTGCGAGTGCGACCACAGAGATCCACCCAGGTTATCCTGTCGTGGTGAACA
ATCCCGAGTTAGTCGCTAGTATGAGGCCTGTGCTTGCCAGTGTGGTGGGCGATAAGATGCTGATTGAACCTGAGTTAATC
ACAGGTGCCGAAGACTTTTCTTATTACGCCTTAGAAGCGCCTGGGATGTTTTTCTTCCTCGGGGTGACGCCAAAGGGAAC
GGATCCAGAAACCGCGGCCAGCAATCATTCTCCGGCTTTTTATGTAGATGAAAGTGCGTTAAAAGTGGGAGTTGAGGCCA
TGACTAAAGTGGCGTTGACTGCGCTAAATGCGCAATAA

Upstream 100 bases:

>100_bases
TGAAGCACAGTCTTTTTTACCTTTATCATTAAATCTATTAACAATTTCTCCTTGTCCGTGTAGTTTAGGTAGCTTATAAA
AACAACAATAGGCTATCGCT

Downstream 100 bases:

>100_bases
GCCACGCAAGCCTTATTCCGTAAGCTCTTCGGTATAACTTGAGCTTACAAAAAATCGGCGCGTGATAAGCATCCAGCGCT
TGATTTGTTAAACAATTTAC

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTLPSVSLAASTSFEFKPLVNKSHFSKTLLASALALSFMGAGLSSAQAATSAMPDAAKLAAGVEQKVIDWRRDLHQHPEL
SNREFRTSKIIEKHLKSLGLEVQTGVAHTGVVAILKGGKLKGGKSGPLIAIRADMDALPVTEVVDLPFASKATDTYRDQK
VGVMHACGHDTHVAMLMGVAENLVKVKDSLAGDVMFIFQPAEEGAPDGEEGGAELMLKEGLFAKRKPDQVFGMHVTSSMP
SGMIGVRSGPAMASEDSFTIKVKGRQTHGSRPWNGVDPIVAAAQIITNVQTIVSRQVDITKAPAVVSFGAVNGGIRSNII
PDEVELIGTIRTFDQPMRADIKVRLAEMAELSAKTLGASATTEIHPGYPVVVNNPELVASMRPVLASVVGDKMLIEPELI
TGAEDFSYYALEAPGMFFFLGVTPKGTDPETAASNHSPAFYVDESALKVGVEAMTKVALTALNAQ

Sequences:

>Translated_465_residues
MTLPSVSLAASTSFEFKPLVNKSHFSKTLLASALALSFMGAGLSSAQAATSAMPDAAKLAAGVEQKVIDWRRDLHQHPEL
SNREFRTSKIIEKHLKSLGLEVQTGVAHTGVVAILKGGKLKGGKSGPLIAIRADMDALPVTEVVDLPFASKATDTYRDQK
VGVMHACGHDTHVAMLMGVAENLVKVKDSLAGDVMFIFQPAEEGAPDGEEGGAELMLKEGLFAKRKPDQVFGMHVTSSMP
SGMIGVRSGPAMASEDSFTIKVKGRQTHGSRPWNGVDPIVAAAQIITNVQTIVSRQVDITKAPAVVSFGAVNGGIRSNII
PDEVELIGTIRTFDQPMRADIKVRLAEMAELSAKTLGASATTEIHPGYPVVVNNPELVASMRPVLASVVGDKMLIEPELI
TGAEDFSYYALEAPGMFFFLGVTPKGTDPETAASNHSPAFYVDESALKVGVEAMTKVALTALNAQ
>Mature_464_residues
TLPSVSLAASTSFEFKPLVNKSHFSKTLLASALALSFMGAGLSSAQAATSAMPDAAKLAAGVEQKVIDWRRDLHQHPELS
NREFRTSKIIEKHLKSLGLEVQTGVAHTGVVAILKGGKLKGGKSGPLIAIRADMDALPVTEVVDLPFASKATDTYRDQKV
GVMHACGHDTHVAMLMGVAENLVKVKDSLAGDVMFIFQPAEEGAPDGEEGGAELMLKEGLFAKRKPDQVFGMHVTSSMPS
GMIGVRSGPAMASEDSFTIKVKGRQTHGSRPWNGVDPIVAAAQIITNVQTIVSRQVDITKAPAVVSFGAVNGGIRSNIIP
DEVELIGTIRTFDQPMRADIKVRLAEMAELSAKTLGASATTEIHPGYPVVVNNPELVASMRPVLASVVGDKMLIEPELIT
GAEDFSYYALEAPGMFFFLGVTPKGTDPETAASNHSPAFYVDESALKVGVEAMTKVALTALNAQ

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=434, Percent_Identity=26.2672811059908, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 49242; Mature: 49111

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPSVSLAASTSFEFKPLVNKSHFSKTLLASALALSFMGAGLSSAQAATSAMPDAAKLA
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
AGVEQKVIDWRRDLHQHPELSNREFRTSKIIEKHLKSLGLEVQTGVAHTGVVAILKGGKL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCC
KGGKSGPLIAIRADMDALPVTEVVDLPFASKATDTYRDQKVGVMHACGHDTHVAMLMGVA
CCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
ENLVKVKDSLAGDVMFIFQPAEEGAPDGEEGGAELMLKEGLFAKRKPDQVFGMHVTSSMP
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHEEECCCCCCCCCCCCEEEEEEECCCC
SGMIGVRSGPAMASEDSFTIKVKGRQTHGSRPWNGVDPIVAAAQIITNVQTIVSRQVDIT
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KAPAVVSFGAVNGGIRSNIIPDEVELIGTIRTFDQPMRADIKVRLAEMAELSAKTLGASA
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TTEIHPGYPVVVNNPELVASMRPVLASVVGDKMLIEPELITGAEDFSYYALEAPGMFFFL
CCEECCCCCEEECCHHHHHHHHHHHHHHHCCCEEECCHHCCCCCCCCEEEEECCCEEEEE
GVTPKGTDPETAASNHSPAFYVDESALKVGVEAMTKVALTALNAQ
EECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLPSVSLAASTSFEFKPLVNKSHFSKTLLASALALSFMGAGLSSAQAATSAMPDAAKLA
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
AGVEQKVIDWRRDLHQHPELSNREFRTSKIIEKHLKSLGLEVQTGVAHTGVVAILKGGKL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCC
KGGKSGPLIAIRADMDALPVTEVVDLPFASKATDTYRDQKVGVMHACGHDTHVAMLMGVA
CCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
ENLVKVKDSLAGDVMFIFQPAEEGAPDGEEGGAELMLKEGLFAKRKPDQVFGMHVTSSMP
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHEEECCCCCCCCCCCCEEEEEEECCCC
SGMIGVRSGPAMASEDSFTIKVKGRQTHGSRPWNGVDPIVAAAQIITNVQTIVSRQVDIT
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KAPAVVSFGAVNGGIRSNIIPDEVELIGTIRTFDQPMRADIKVRLAEMAELSAKTLGASA
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TTEIHPGYPVVVNNPELVASMRPVLASVVGDKMLIEPELITGAEDFSYYALEAPGMFFFL
CCEECCCCCEEECCHHHHHHHHHHHHHHHCCCEEECCHHCCCCCCCCEEEEECCCEEEEE
GVTPKGTDPETAASNHSPAFYVDESALKVGVEAMTKVALTALNAQ
EECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]