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The map label for this gene is acnB [H]

Identifier: 160877150

GI number: 160877150

Start: 4767424

End: 4770096

Strand: Reverse

Name: acnB [H]

Synonym: Sbal195_4046

Alternate gene names: 160877150

Gene position: 4770096-4767424 (Counterclockwise)

Preceding gene: 160877153

Following gene: 160877149

Centisome position: 89.21

GC content: 48.37

Gene sequence:

>2673_bases
ATGTCGGACTGTCAGCTTCAATTAAGAAGACGTGCAGTTTCGTTGTTAGAGCGCCAAACAAAGAGGAATGTTGCCGTGCT
AGAAGCATATCGTAAACACGTCGCAGAACGTGCTGCAGAGGGCGTAGTCCCTAAGCCATTAGATGCACATCAAGTGGCTG
AACTGGTTAAGTTAGTTCAAAACCCACCCGCAGGTGAAGAGGCTTTCGTTCTCGACCTGCTTGAAAATCGAATTCCCCCA
GGTGTTGATGAAGCTGCTTATGTTAAAGCTGCTTTTTTAGATGCGGTTGCCAAAGGCACTGAAAAATCACCGATTCTGTC
TGCTGAGCGTGCTACTGAGTTGTTAGGCACAATGCAAGGCGGCTACAACATCGAACCTCTGATCGCGCAGCTGGATAACC
CAGCGTTAGCACCTTTAGCCGTTAAAGCTTTATCTCACACTCTGCTAATGTTTGATTCTTTCCACGATGTGGTTGAGAAG
ATGGAAGCGGGCAATGAGTTTGCTAAGCAAGTGGTTGAAGCGTGGGCAAATGCAGATTGGTATTTAAGCCGTCCAAAACT
GGCCGACAAAGTCACTCTGACAGTATTTAAAGTTTCTGGTGAAACCAACACCGATGACTTGTCACCTGCACCAGATGCAT
GGTCACGTCCTGATATTCCATTACACGCGTTAGCTATGCTGAAAAACGCCCGTGATGGTATCGAGCCTGACGTTGCTGGC
GCCGTTGGCCCAGTGAAAAAAATCGAAGCGTTAAAAGCGCAAGGTTTCCCATTAGTGTATGTGGGCGACGTTGTGGGAAC
AGGTTCATCACGTAAATCAGCGACTAACTCAGTACTGTGGTTTATGGGCGATGACATTCCATTCGTACCTAACAAGCGCG
CTGGTGGTTTCTGCCTTGGCGGTAAAATTGCACCGATTTTCTTCAACACTATGGAAGATGCGGGCGCACTGCCAATCGAG
CTTGACGTAAACAAAATGGAAATGGGCGATGTGATTGACATCTACCCATACGCAGGTGTGGTTAAGCGTCACAACAGCGA
CGAAGTGATTTCAGAATTCAGCTTAAAAACTGAAGTGCTGTTAGACGAAGTGCGTGCTGGTGGTCGTATTCCACTGATCA
TCGGTCGTGGTTTAACTGACAAAGCCCGTGAAGTATTAGGTTTAGGCGTATCTGACGTATTCGTGCGTTCACAAGACGTG
GCTGATACTGGTAAGGGTTACACTCTAGCGCAGAAGATGGTCGGTAAAGCATGCGGCGTAGCTGGCGTGCGTCCTGGCCA
ATACTGCGAACCTAAGATGACCTCTGTTGGTTCACAAGACACCACAGGTCCTATGACCCGTGACGAGCTGAAAGATTTAG
CTTGCTTAGGTTTCAGTGCTGATTTGACTATGCAGTCATTCTGTCACACTGCGGCTTATCCAAAGCCTGTTGATGTGAAC
ACACATCATACTCTGCCAGATTTCATCATGAACCGCGGCGGTGTATCACTGCGTCCAGGCGACGGTGTTATCCACTCATG
GTTAAACCGTATGCTGCTGCCAGATACCGTTGGTACGGGTGGTGACTCACATACGCGTTTCCCAATTGGTATTTCATTCC
CAGCTGGTTCTGGCTTAGTGGCGTTTGCTGCGGCGACTGGCGTTATGCCACTCGACATGCCTGAATCAGTATTAGTGCGC
TTCAAGGGCAAAATGCAACCCGGCATCACACTGCGTGATCTGGTACATGCTATCCCATTAAAAGCCATTGAACTGGGCAT
GCTGACCGTTGAGAAGAAAGGTAAAGTCAACATCTTCTCTGGTCGCGTACTGGAAATCGAAGGTCTTGAAACCTTGAAAG
TTGAACAAGCTTTCGAACTGTCTGATGCTTCAGCTGAGCGCTCTGCTGCCGGTTGTACCATCAAGTTAGACAAAGAACCT
ATCATCGAATACCTGAACTCAAACATCATCATGCTGAAGTGGATGATCGCTGAAGGTTACGGCGATCGTCGTACGATTGA
GCGTCGTATCAAGGGCATGGAAGAGTGGTTAGCGAATCCTGAACTGATGAGCGCCGATAAAGATGCTGAGTACGCTGCAG
TTATCGAAATCGATTTAAACGACATCAAAGAGCCAATCCTGTGTGCGCCAAACGATCCTGATGATGCAGTACTGCTGTCA
TCTGTTGCGCAAACTAAGATTGACGAAGTGTTCGTCGGTTCTTGTATGACCAACATTGGTCACTTCCGCGCAACCGGTAA
GATGTTAGACAAGTTCGCTAAAACCCTGCCAACCCGTCTGTGGATTGCTCCACCGACTAAGATGGACAGAGATCAGCTGA
CTGAAGAAGGCTACTACGCGATTTTCGGTCGTGTAGGCGCGCGCATCGAGATCCCAGGCTGTTCACTGTGTATGGGTAAC
CAAGCACGTGTAGCTGAAGGTGCAACTGTCGTATCGACTTCTACCCGTAACTTCCCTAACCGTTTAGGTACAGGCGCTAA
CGTTTACTTAGCCTCTGCTGAACTGGCTGCGGTTGCTGCACTGTTAGGTCGTCTGCCGTCACCTGAAGAATATCAAGAAT
ACGCAAAAGAGTTAGACGCCACAGCGGCTGACACTTACCGTTACTTGAACTTCGATCAAATCGAGTCTTATACACAAAAG
GCATCGGAAGTGATCTTCCAAACTACTGTGTAA

Upstream 100 bases:

>100_bases
GCCGCCGGATTTAGTCCTTATTTCGCTTTTTGCCGAGTGTGACCTGATTCATACTTTTGCAAAGCTGTAGTATACTGCAG
GCCGGAAATACGGGTCGATC

Downstream 100 bases:

>100_bases
TCGATTGCCGTTAAGGTAGCTTGATGTACAAAAGCCAGCGCAAGCTGGCTTTTTTGTAGGTCAGTTTCTCCTCCACATAT
ATATGTACAAATAACCCCTT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 890; Mature: 889

Protein sequence:

>890_residues
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPP
GVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEK
MEAGNEFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIE
LDVNKMEMGDVIDIYPYAGVVKRHNSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDV
ADTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
FKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEP
IIEYLNSNIIMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGN
QARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQK
ASEVIFQTTV

Sequences:

>Translated_890_residues
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPP
GVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEK
MEAGNEFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIE
LDVNKMEMGDVIDIYPYAGVVKRHNSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDV
ADTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
FKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEP
IIEYLNSNIIMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGN
QARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQK
ASEVIFQTTV
>Mature_889_residues
SDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPPG
VDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKM
EAGNEFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAGA
VGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIEL
DVNKMEMGDVIDIYPYAGVVKRHNSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDVA
DTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNT
HHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRF
KGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPI
IEYLNSNIIMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLSS
VAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQ
ARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQKA
SEVIFQTTV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=81.0404624277457, Blast_Score=1445, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=386, Percent_Identity=25.3886010362694, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=26.509186351706, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 96546; Mature: 96415

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQ
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPPAGEEAFVLDLLENRIPPGVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADW
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
YLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
EECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLG
CCCHHHHHHHHHCCCCCEEEECCEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEC
GKIAPIFFNTMEDAGALPIELDVNKMEMGDVIDIYPYAGVVKRHNSDEVISEFSLKTEVL
CEEEEEEEECHHCCCCCEEEEECCCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHH
LDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDVADTGKGYTLAQKMVGKACGV
HHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
AGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
CCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLV
CCCCCHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCE
AFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFS
EEHHHCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHCCEEEEECCCCEEEEC
GRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIIMLKWMIAEGY
CEEEEECCCCEEEHHHHHCCCCCCCHHCCCCCEEEECHHHHHHHHCCCEEEEEEEEECCC
GDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHH
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEE
IFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAA
EEECCCCEEECCCCCEECCCCCCCCCCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHH
LLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQKASEVIFQTTV
HHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPPAGEEAFVLDLLENRIPPGVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADW
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
YLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
EECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLG
CCCHHHHHHHHHCCCCCEEEECCEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEC
GKIAPIFFNTMEDAGALPIELDVNKMEMGDVIDIYPYAGVVKRHNSDEVISEFSLKTEVL
CEEEEEEEECHHCCCCCEEEEECCCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHH
LDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDVADTGKGYTLAQKMVGKACGV
HHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
AGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
CCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLV
CCCCCHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCE
AFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFS
EEHHHCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHCCEEEEECCCCEEEEC
GRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIIMLKWMIAEGY
CEEEEECCCCEEEHHHHHCCCCCCCHHCCCCCEEEECHHHHHHHHCCCEEEEEEEEECCC
GDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHH
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEE
IFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAA
EEECCCCEEECCCCCEECCCCCCCCCCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHH
LLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQKASEVIFQTTV
HHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]