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The map label for this gene is nrfC [H]

Identifier: 160876964

GI number: 160876964

Start: 4547686

End: 4548468

Strand: Direct

Name: nrfC [H]

Synonym: Sbal195_3860

Alternate gene names: 160876964

Gene position: 4547686-4548468 (Clockwise)

Preceding gene: 160876956

Following gene: 160876965

Centisome position: 85.05

GC content: 53.38

Gene sequence:

>783_bases
ATGGACAAGAGTAAACGTCAGTTTCTCGGTAAAATGGCCAGCGTCAGTGTTGGCGCCGCACTCATCCCTGTGGTGCAAGT
GTATGCGCAGGATAATCAACGCGGCCCTCAAGTGCACAGCGCCAGCGAAGGCCAAGTGGATAAACGTTACGGTATGGTAA
TTGACCTACGCCGCTGCGTGGGCTGCCAAGCCTGTACCGTCGCTTGCACTATCGAGAATCAACCGCCCCTAGGGCAATTT
CGCACCACAGTGCAGCAGTATGAAGTTTCGCAGCAGGAAGATACCACGCCGCCTGCGTTTTTAATGCTACCAAGACTTTG
TAATCACTGCGAAAACCCGCCCTGCATCCCAGTTTGTCCTACGGGCGCTACCTTCCAGCGCAAAGACGGCATAGTGGTAG
TAAATAATGAATGGTGCGTTGGCTGTGGCTACTGCGTGCAAGCCTGTCCCTATGATGCGCGCTTTATCAACCACGATACC
AATACCGCCGACAAATGTACCTTCTGCGCCCACAGGTTAGATGCAGGGTTACTGCCCGCCTGTGTCGAAACCTGCGTAGG
AGAAGCCCGTGTGATTGGCGATCTGAATAATCCCAACAGCCAGATAAGTCAACTGCTCGCCACCAATAAAACCGATATCA
AGGTACTCAAACCCACGGCCAATACTGCGCCACGGGTGTTCTACATCGGCATGAGCAGCGCTTTTGAGCACAAGATCAGC
GGCCAAGCCCCCGTTAGAACCTTGCTTACCGATACTGGCGAGGAGATCCACCATGGTCATTAA

Upstream 100 bases:

>100_bases
GCCTCGCCTTTTACAGAATGCCTGTAAACCTCGCCATAAACCTTAACGCTAGGTCTATGAGCCAGTTTATTCGCCATCTG
CAAGAGTGAAGGAAATCTGT

Downstream 100 bases:

>100_bases
AGAAATCTTTACCCCAGGTATCGCCATTACTTGGCTACCTTGGGCGGTGCAATACTTTTTTATGATGGGTCTGGCCTATG
CCAGTGTGTGGATCGCGACA

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDKSKRQFLGKMASVSVGAALIPVVQVYAQDNQRGPQVHSASEGQVDKRYGMVIDLRRCVGCQACTVACTIENQPPLGQF
RTTVQQYEVSQQEDTTPPAFLMLPRLCNHCENPPCIPVCPTGATFQRKDGIVVVNNEWCVGCGYCVQACPYDARFINHDT
NTADKCTFCAHRLDAGLLPACVETCVGEARVIGDLNNPNSQISQLLATNKTDIKVLKPTANTAPRVFYIGMSSAFEHKIS
GQAPVRTLLTDTGEEIHHGH

Sequences:

>Translated_260_residues
MDKSKRQFLGKMASVSVGAALIPVVQVYAQDNQRGPQVHSASEGQVDKRYGMVIDLRRCVGCQACTVACTIENQPPLGQF
RTTVQQYEVSQQEDTTPPAFLMLPRLCNHCENPPCIPVCPTGATFQRKDGIVVVNNEWCVGCGYCVQACPYDARFINHDT
NTADKCTFCAHRLDAGLLPACVETCVGEARVIGDLNNPNSQISQLLATNKTDIKVLKPTANTAPRVFYIGMSSAFEHKIS
GQAPVRTLLTDTGEEIHHGH
>Mature_260_residues
MDKSKRQFLGKMASVSVGAALIPVVQVYAQDNQRGPQVHSASEGQVDKRYGMVIDLRRCVGCQACTVACTIENQPPLGQF
RTTVQQYEVSQQEDTTPPAFLMLPRLCNHCENPPCIPVCPTGATFQRKDGIVVVNNEWCVGCGYCVQACPYDARFINHDT
NTADKCTFCAHRLDAGLLPACVETCVGEARVIGDLNNPNSQISQLLATNKTDIKVLKPTANTAPRVFYIGMSSAFEHKIS
GQAPVRTLLTDTGEEIHHGH

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=234, Percent_Identity=41.4529914529915, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=41.1042944785276, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787122, Length=189, Percent_Identity=36.5079365079365, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=35.978835978836, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787749, Length=197, Percent_Identity=31.9796954314721, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787478, Length=134, Percent_Identity=39.5522388059701, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787740, Length=139, Percent_Identity=36.6906474820144, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790326, Length=197, Percent_Identity=31.9796954314721, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789370, Length=195, Percent_Identity=30.2564102564103, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787963, Length=185, Percent_Identity=25.9459459459459, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789067, Length=187, Percent_Identity=31.5508021390374, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788811, Length=176, Percent_Identity=31.25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082179, Length=142, Percent_Identity=32.3943661971831, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082180, Length=143, Percent_Identity=32.1678321678322, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=30.188679245283, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28380; Mature: 28380

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSKRQFLGKMASVSVGAALIPVVQVYAQDNQRGPQVHSASEGQVDKRYGMVIDLRRCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCEEEHHHHC
GCQACTVACTIENQPPLGQFRTTVQQYEVSQQEDTTPPAFLMLPRLCNHCENPPCIPVCP
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECC
TGATFQRKDGIVVVNNEWCVGCGYCVQACPYDARFINHDTNTADKCTFCAHRLDAGLLPA
CCCCEECCCCEEEECCCEEEECCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCHHHH
CVETCVGEARVIGDLNNPNSQISQLLATNKTDIKVLKPTANTAPRVFYIGMSSAFEHKIS
HHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCHHHHHHCC
GQAPVRTLLTDTGEEIHHGH
CCCHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MDKSKRQFLGKMASVSVGAALIPVVQVYAQDNQRGPQVHSASEGQVDKRYGMVIDLRRCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCEEEHHHHC
GCQACTVACTIENQPPLGQFRTTVQQYEVSQQEDTTPPAFLMLPRLCNHCENPPCIPVCP
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECC
TGATFQRKDGIVVVNNEWCVGCGYCVQACPYDARFINHDTNTADKCTFCAHRLDAGLLPA
CCCCEECCCCEEEECCCEEEECCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCHHHH
CVETCVGEARVIGDLNNPNSQISQLLATNKTDIKVLKPTANTAPRVFYIGMSSAFEHKIS
HHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCHHHHHHCC
GQAPVRTLLTDTGEEIHHGH
CCCHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]