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The map label for this gene is ycdT [H]

Identifier: 160876864

GI number: 160876864

Start: 4428517

End: 4430535

Strand: Direct

Name: ycdT [H]

Synonym: Sbal195_3760

Alternate gene names: 160876864

Gene position: 4428517-4430535 (Clockwise)

Preceding gene: 160876861

Following gene: 160876866

Centisome position: 82.82

GC content: 43.24

Gene sequence:

>2019_bases
ATGCAAAATTGCGATACATCTATATTCAAAATGAACAAACAAGTTATGCTAAGCTCACTATTAGCTATTGATTTTAATGT
GAACAGGATACCTTTTTTGCTACGTCCATTCCGATACTTATCATTTACCTTGTTGCTGCTCTTACTGCTGCTCAGCCTCG
CTATGGCAGAAGAAAAGACTCAGGTTGATAAAATATTTGAAAGACTTGAAAGCGGTGAGCCCATGACCCGCGAAGCAATT
AACAACGATCTTGCATTATTGAGCAAACTGATTGCAACCGACGATGTACTCCGCAATGAAAAGCTACAGCTACTAAAATG
CTGGGAGCAAAGTGCCGAAACGGATACAGACATAGCACAGGCAGTTAAGAATACGAGTCAAACACTCACCCTTATTCCAC
CAACGGCTTCCGCGCATTTCATCACCGACCTCATGCTTTGTCATTCTTGGTTTTTGCAGTTAAATGGTGATGTAGATAGC
GCAATGGAAGGCTATAACCTCAGCGTTAAGCGTGCCTATGAGCATGAGGATCTTAAACTCATTGCCGACTCACGCAGCCT
TCGCGGCGCTCTGTATTCCTATATTGGCGATTTTTCTGCGGCATTAGACGATCTGATAACTGCACAAGATCTCTACGAGT
CACTGAATTTAACCGGCTGGGCTAACATCAATCTTGCCGATATGGCCACCAGCTTTAGACGTTATGGCGATCCCCAAAGT
GCAATCCGTTACTACAATAAACTCAAAGCACTCTATTTAAAGAACAATAACCAAGAGCAGGCAATGTATGTCACCTCAGA
AATAGGTCTGGCTTTAGATGAACTGGGTGAACATCAACAAGCTATTGATCACTTCTTGCTCAGCTATCAATACTTTAAGG
AACATCAGCTAGAAAAAGTGGCCGCCACTATGGCGACGGACATTGCCTTTAGCTTAATCAAACTCCATCGTCTTAATGAA
GCGGAAAAATATTTAACTGAAGCCGCAGCTGTGATTAGCGAAAAAGATTATGCCGCTTATAGCTTTATGAAACTCTTTAC
GGCCAATATCAAGTTTCAACAAGCACGTTATAAAGAAGCGCTTACTGAACTCGAACAGGGGGAAAAAGCCTTTCGAACTC
TGCAAAATGATCGGGGACTCACCCAACTATTACAGCTGAAAAGTGATGTCTATGCGGCGATGAATAATTTACCTGCGGCC
TATGGCGCACTGCAGGAATTTGTCGCCCTCACCAAAAAAGTCGATGACAACTCCTTCTCTCATCAAACCACAGAATTAAA
AATGAAGTTTGATACCACTAGGATTGAATCTGAAAATCAGCGCTTGATTGAAAACCAAAGCCTTAAAGAACAAGAACTCG
CGCTGCTTGAGAAGAATAAGTCATTGCAACACATCATTTTATTATTGACGGGCTTTATCCTAACAATAGTGTCGATATTT
GCGTATAAACAAGTGCAGAGAAATCGCCAATTACAGGTCATAGCCCTCACCGATTACCTCACCAAGCTCCCTAATCGTCG
GCATATTTATGCCCAAGCCGCGCAGTATTTTCAGCAAGCCTTAAAACAACAAAGCCCTTTTTCGGTGATTATCTTTGATG
CGGACCACTTCAAGAAAATTAACGATAACTTCGGCCATGAACTGGGCGATCGCGCCTTACTGACGATCGCAAGCGCAAGT
CAAGCCCTAACGGGTAATAAAGATCTTATTGGCCGTATCGGTGGTGAAGAGTTTTTAATTCTCTTACCCAACACAGATGC
TGAACGTGCTTGTGAACTTGCCCATCAGTTGCAAGCTCATATTAGCCGCCTAGGCGCAGAGGATTTACCCGCTGAACTCA
AATTGACTGTTAGCGCAGGAGTCGCAACCTTAGCGCCACAAAAAAAGACTGAAAATACCTATCAAGACAAAGACTTTGCC
AGCCTACTGAAACGTGCCGACGACGCCCTTTATGAGGCTAAAAATGCGGGAAGGAATTGTGTTAAAGCTGCCAGAAATGA
AGCTGTGAGCGAGAGTTAA

Upstream 100 bases:

>100_bases
CCGCTCATCATGAGTAGGGCAGCACAAAGTAATTTTTTCAAGGGTGATTCCTTTATAGGATAATTTCCAAGGCGAAGTTT
ATACCTTGATAACTAACTGA

Downstream 100 bases:

>100_bases
ACGCTTTTATGCCAAAACACTTGATGCTGTAATGGCGGCGGTAAAGCACCAAACCATGTCTGATATAAACGACTAAACTC
CCCTGAGTCTTTCATTTTCT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MQNCDTSIFKMNKQVMLSSLLAIDFNVNRIPFLLRPFRYLSFTLLLLLLLLSLAMAEEKTQVDKIFERLESGEPMTREAI
NNDLALLSKLIATDDVLRNEKLQLLKCWEQSAETDTDIAQAVKNTSQTLTLIPPTASAHFITDLMLCHSWFLQLNGDVDS
AMEGYNLSVKRAYEHEDLKLIADSRSLRGALYSYIGDFSAALDDLITAQDLYESLNLTGWANINLADMATSFRRYGDPQS
AIRYYNKLKALYLKNNNQEQAMYVTSEIGLALDELGEHQQAIDHFLLSYQYFKEHQLEKVAATMATDIAFSLIKLHRLNE
AEKYLTEAAAVISEKDYAAYSFMKLFTANIKFQQARYKEALTELEQGEKAFRTLQNDRGLTQLLQLKSDVYAAMNNLPAA
YGALQEFVALTKKVDDNSFSHQTTELKMKFDTTRIESENQRLIENQSLKEQELALLEKNKSLQHIILLLTGFILTIVSIF
AYKQVQRNRQLQVIALTDYLTKLPNRRHIYAQAAQYFQQALKQQSPFSVIIFDADHFKKINDNFGHELGDRALLTIASAS
QALTGNKDLIGRIGGEEFLILLPNTDAERACELAHQLQAHISRLGAEDLPAELKLTVSAGVATLAPQKKTENTYQDKDFA
SLLKRADDALYEAKNAGRNCVKAARNEAVSES

Sequences:

>Translated_672_residues
MQNCDTSIFKMNKQVMLSSLLAIDFNVNRIPFLLRPFRYLSFTLLLLLLLLSLAMAEEKTQVDKIFERLESGEPMTREAI
NNDLALLSKLIATDDVLRNEKLQLLKCWEQSAETDTDIAQAVKNTSQTLTLIPPTASAHFITDLMLCHSWFLQLNGDVDS
AMEGYNLSVKRAYEHEDLKLIADSRSLRGALYSYIGDFSAALDDLITAQDLYESLNLTGWANINLADMATSFRRYGDPQS
AIRYYNKLKALYLKNNNQEQAMYVTSEIGLALDELGEHQQAIDHFLLSYQYFKEHQLEKVAATMATDIAFSLIKLHRLNE
AEKYLTEAAAVISEKDYAAYSFMKLFTANIKFQQARYKEALTELEQGEKAFRTLQNDRGLTQLLQLKSDVYAAMNNLPAA
YGALQEFVALTKKVDDNSFSHQTTELKMKFDTTRIESENQRLIENQSLKEQELALLEKNKSLQHIILLLTGFILTIVSIF
AYKQVQRNRQLQVIALTDYLTKLPNRRHIYAQAAQYFQQALKQQSPFSVIIFDADHFKKINDNFGHELGDRALLTIASAS
QALTGNKDLIGRIGGEEFLILLPNTDAERACELAHQLQAHISRLGAEDLPAELKLTVSAGVATLAPQKKTENTYQDKDFA
SLLKRADDALYEAKNAGRNCVKAARNEAVSES
>Mature_672_residues
MQNCDTSIFKMNKQVMLSSLLAIDFNVNRIPFLLRPFRYLSFTLLLLLLLLSLAMAEEKTQVDKIFERLESGEPMTREAI
NNDLALLSKLIATDDVLRNEKLQLLKCWEQSAETDTDIAQAVKNTSQTLTLIPPTASAHFITDLMLCHSWFLQLNGDVDS
AMEGYNLSVKRAYEHEDLKLIADSRSLRGALYSYIGDFSAALDDLITAQDLYESLNLTGWANINLADMATSFRRYGDPQS
AIRYYNKLKALYLKNNNQEQAMYVTSEIGLALDELGEHQQAIDHFLLSYQYFKEHQLEKVAATMATDIAFSLIKLHRLNE
AEKYLTEAAAVISEKDYAAYSFMKLFTANIKFQQARYKEALTELEQGEKAFRTLQNDRGLTQLLQLKSDVYAAMNNLPAA
YGALQEFVALTKKVDDNSFSHQTTELKMKFDTTRIESENQRLIENQSLKEQELALLEKNKSLQHIILLLTGFILTIVSIF
AYKQVQRNRQLQVIALTDYLTKLPNRRHIYAQAAQYFQQALKQQSPFSVIIFDADHFKKINDNFGHELGDRALLTIASAS
QALTGNKDLIGRIGGEEFLILLPNTDAERACELAHQLQAHISRLGAEDLPAELKLTVSAGVATLAPQKKTENTYQDKDFA
SLLKRADDALYEAKNAGRNCVKAARNEAVSES

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=191, Percent_Identity=33.5078534031414, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=34.7826086956522, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=36.8131868131868, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=39.1061452513966, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=36.5269461077844, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693134, Length=225, Percent_Identity=29.3333333333333, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=30.4878048780488, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081977, Length=199, Percent_Identity=31.1557788944724, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=30.4597701149425, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=29.8913043478261, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788085, Length=192, Percent_Identity=26.5625, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081974, Length=143, Percent_Identity=25.1748251748252, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 76004; Mature: 76004

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNCDTSIFKMNKQVMLSSLLAIDFNVNRIPFLLRPFRYLSFTLLLLLLLLSLAMAEEKT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVDKIFERLESGEPMTREAINNDLALLSKLIATDDVLRNEKLQLLKCWEQSAETDTDIAQ
HHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AVKNTSQTLTLIPPTASAHFITDLMLCHSWFLQLNGDVDSAMEGYNLSVKRAYEHEDLKL
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCHHE
IADSRSLRGALYSYIGDFSAALDDLITAQDLYESLNLTGWANINLADMATSFRRYGDPQS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHH
AIRYYNKLKALYLKNNNQEQAMYVTSEIGLALDELGEHQQAIDHFLLSYQYFKEHQLEKV
HHHHHHHHHEEEEECCCCCHHEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AATMATDIAFSLIKLHRLNEAEKYLTEAAAVISEKDYAAYSFMKLFTANIKFQQARYKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LTELEQGEKAFRTLQNDRGLTQLLQLKSDVYAAMNNLPAAYGALQEFVALTKKVDDNSFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
HQTTELKMKFDTTRIESENQRLIENQSLKEQELALLEKNKSLQHIILLLTGFILTIVSIF
CCCEEEEEEECHHHCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AYKQVQRNRQLQVIALTDYLTKLPNRRHIYAQAAQYFQQALKQQSPFSVIIFDADHFKKI
HHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
NDNFGHELGDRALLTIASASQALTGNKDLIGRIGGEEFLILLPNTDAERACELAHQLQAH
HHHHCHHHCCHHEEEEHHHHHHHCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
ISRLGAEDLPAELKLTVSAGVATLAPQKKTENTYQDKDFASLLKRADDALYEAKNAGRNC
HHHCCCCCCCCCEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
VKAARNEAVSES
HHHHHHHCCCCC
>Mature Secondary Structure
MQNCDTSIFKMNKQVMLSSLLAIDFNVNRIPFLLRPFRYLSFTLLLLLLLLSLAMAEEKT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVDKIFERLESGEPMTREAINNDLALLSKLIATDDVLRNEKLQLLKCWEQSAETDTDIAQ
HHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AVKNTSQTLTLIPPTASAHFITDLMLCHSWFLQLNGDVDSAMEGYNLSVKRAYEHEDLKL
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCHHE
IADSRSLRGALYSYIGDFSAALDDLITAQDLYESLNLTGWANINLADMATSFRRYGDPQS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHH
AIRYYNKLKALYLKNNNQEQAMYVTSEIGLALDELGEHQQAIDHFLLSYQYFKEHQLEKV
HHHHHHHHHEEEEECCCCCHHEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AATMATDIAFSLIKLHRLNEAEKYLTEAAAVISEKDYAAYSFMKLFTANIKFQQARYKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LTELEQGEKAFRTLQNDRGLTQLLQLKSDVYAAMNNLPAAYGALQEFVALTKKVDDNSFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
HQTTELKMKFDTTRIESENQRLIENQSLKEQELALLEKNKSLQHIILLLTGFILTIVSIF
CCCEEEEEEECHHHCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AYKQVQRNRQLQVIALTDYLTKLPNRRHIYAQAAQYFQQALKQQSPFSVIIFDADHFKKI
HHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
NDNFGHELGDRALLTIASASQALTGNKDLIGRIGGEEFLILLPNTDAERACELAHQLQAH
HHHHCHHHCCHHEEEEHHHHHHHCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
ISRLGAEDLPAELKLTVSAGVATLAPQKKTENTYQDKDFASLLKRADDALYEAKNAGRNC
HHHCCCCCCCCCEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
VKAARNEAVSES
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]