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The map label for this gene is ahpF [H]

Identifier: 160876745

GI number: 160876745

Start: 4289152

End: 4290738

Strand: Direct

Name: ahpF [H]

Synonym: Sbal195_3639

Alternate gene names: 160876745

Gene position: 4289152-4290738 (Clockwise)

Preceding gene: 160876744

Following gene: 160876751

Centisome position: 80.21

GC content: 50.35

Gene sequence:

>1587_bases
ATGTTAGATGCGAATTTAAAAAATCAACTGCAAACCTATCTGCAAAACCTCAAGAGACCAGTTGAACTTGTCGTGTCTGC
AGATGAGAGCAAAAAGTCTCAAGAATTAAAAAGCTTAGCGCAAGACATTGTCAGCCTATCTTCTCTGGTGAGCCTGAAAG
AAACTCAGGGCGAACGCACCCCTGCGATGACAGTGATAAACCCACAACTTAATACGCAAATCAGCTTTGCTGGTCTGCCT
ATGGGTCACGAATTTACCTCACTTGTGTTGGCCTTACTGCACACGGGTGGACACCCAATCAAACTCAGCGATGACATTAT
CGAGCAGATCCGCAATCTGCCGGGCAAATATGCCTTTGAAACTTACGTCTCACTGACCTGCCAAAACTGCCCAGACGTAG
TACAGGCACTGAACATGATGGCGGCGATTAACCCGAATATCACCAACGTGATGATCGACGGCGCCCTGTTCCAAGACGAA
GTGGCGAACCGTAACATTATGGCCGTGCCTTCTGTCTACTTAAATGGCGAAGTGTTTGCAGCCGGTCGTATCAGCATTGG
CGAGATCCTCAATAAACTGGACACCAATGCCGCAAGCCGCAAGGCCGATGAAATTAATGAAAAAGCGCCATTCGAAGTCT
TAGTGGTAGGCGGCGGCCCAGCGGGCGCAGCGGCAGCTATTTATGCAGCACGTAAAGGCCTTCGCACTGGTATTGTTGCC
GATAAGTTTGGCGGCCAAGTGGCTGAAACCGTTGGTATCGAGAACTTTATTTCGGTCAAAGCGACCGAAGGTCCAAAGCT
AGTGGCAAACCTTGAAGCCCATGTACGAGACTACGAAGTCGACATCATGGACAACCAAAAGGCAGTGTCGTTATCAACAG
ATGGCTTGTTCGAGCTTGAACTGGCAAGCGGCGCTAAATTACGTAGCCGTACCGTATTGTTAGCCACAGGTGCACGCTGG
CGAGAAATGAACGTCCCCGGCGAGAAAGAATACCGTGGTAAAGGTGTCGCATACTGCCCGCATTGCGATGGCCCGTTATT
TAAAGGCAAGCGTGTGGCGGTGATTGGCGGCGGTAACTCAGGTATCGAAGCGGCTATCGATCTCGCCAACATAGTAGAAC
ATGTGACTGTGCTGGAGTTTGATAAAACCCTACGCGCCGACGATGTGCTACAACGTAAAGCAGCGTCTATGGGTAACATC
AAAATTATTACCCAAGCCATGACCACAGAAGTGACTGGCGATGGTACTCGGGTTAATGGCCTGAACTACACAGACAGAGC
AACAGGCGAGAGTCACCATGTCGCACTTGCAGGTATCTTCGTACAGATTGGGCTAGTGCCGAATACCGAGTGGCTAAAAG
GTGTGGTTGAACTGACGCCACGTGGGGAAATCATTGTCGATGCCCGCGGTCAAACATCCATCCCTGGAGTATTTGCGGCA
GGCGATGTCACTGACACGCCGTTTAAGCAAATTATCATCGCCATGGGCAGCGGCGCGAGTGCATCACTGGGCGCATTTGA
TTACCTAATTCGCCACAGCGATGAAGAAATCGCCGAAACGAAAACCACAGACACGAAAGCGGCCTAA

Upstream 100 bases:

>100_bases
CACAGTTGTAGCGCCACGCAGCAAAGGATTAAGCCCGCCCCTGCAAGGGCCTAGACTGGCCCCCTTATCTCTCCCTTTTA
CGCGATTTTGGAGTCATCAC

Downstream 100 bases:

>100_bases
TTGTTAAACATTAACCGCATAACCTAAGAAGCCAGCGCAAGCTGGCTTCTTTTGCATCGAATAAATAAAATTTTTCACAA
AGAACGAGTTAAAGCTTCCT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLAQDIVSLSSLVSLKETQGERTPAMTVINPQLNTQISFAGLP
MGHEFTSLVLALLHTGGHPIKLSDDIIEQIRNLPGKYAFETYVSLTCQNCPDVVQALNMMAAINPNITNVMIDGALFQDE
VANRNIMAVPSVYLNGEVFAAGRISIGEILNKLDTNAASRKADEINEKAPFEVLVVGGGPAGAAAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVSLSTDGLFELELASGAKLRSRTVLLATGARW
REMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDKTLRADDVLQRKAASMGNI
KIITQAMTTEVTGDGTRVNGLNYTDRATGESHHVALAGIFVQIGLVPNTEWLKGVVELTPRGEIIVDARGQTSIPGVFAA
GDVTDTPFKQIIIAMGSGASASLGAFDYLIRHSDEEIAETKTTDTKAA

Sequences:

>Translated_528_residues
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLAQDIVSLSSLVSLKETQGERTPAMTVINPQLNTQISFAGLP
MGHEFTSLVLALLHTGGHPIKLSDDIIEQIRNLPGKYAFETYVSLTCQNCPDVVQALNMMAAINPNITNVMIDGALFQDE
VANRNIMAVPSVYLNGEVFAAGRISIGEILNKLDTNAASRKADEINEKAPFEVLVVGGGPAGAAAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVSLSTDGLFELELASGAKLRSRTVLLATGARW
REMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDKTLRADDVLQRKAASMGNI
KIITQAMTTEVTGDGTRVNGLNYTDRATGESHHVALAGIFVQIGLVPNTEWLKGVVELTPRGEIIVDARGQTSIPGVFAA
GDVTDTPFKQIIIAMGSGASASLGAFDYLIRHSDEEIAETKTTDTKAA
>Mature_528_residues
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLAQDIVSLSSLVSLKETQGERTPAMTVINPQLNTQISFAGLP
MGHEFTSLVLALLHTGGHPIKLSDDIIEQIRNLPGKYAFETYVSLTCQNCPDVVQALNMMAAINPNITNVMIDGALFQDE
VANRNIMAVPSVYLNGEVFAAGRISIGEILNKLDTNAASRKADEINEKAPFEVLVVGGGPAGAAAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVSLSTDGLFELELASGAKLRSRTVLLATGARW
REMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDKTLRADDVLQRKAASMGNI
KIITQAMTTEVTGDGTRVNGLNYTDRATGESHHVALAGIFVQIGLVPNTEWLKGVVELTPRGEIIVDARGQTSIPGVFAA
GDVTDTPFKQIIIAMGSGASASLGAFDYLIRHSDEEIAETKTTDTKAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=58.027079303675, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=36.1290322580645, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=34.6153846153846, Blast_Score=153, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=337, Percent_Identity=31.7507418397626, Blast_Score=150, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56439; Mature: 56439

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLAQDIVSLSSLVSLKETQGERT
CCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAMTVINPQLNTQISFAGLPMGHEFTSLVLALLHTGGHPIKLSDDIIEQIRNLPGKYAFE
CEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHCCCCCEEEE
TYVSLTCQNCPDVVQALNMMAAINPNITNVMIDGALFQDEVANRNIMAVPSVYLNGEVFA
HEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCEEEECEEEECCEEEE
AGRISIGEILNKLDTNAASRKADEINEKAPFEVLVVGGGPAGAAAAIYAARKGLRTGIVA
ECCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVSLSTDGLFELE
HHCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHCEEEEEEEECCCEEEEEECCCEEEEE
LASGAKLRSRTVLLATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNS
ECCCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDKTLRADDVLQRKAASMGNIKIITQAMTTEVTGDGTRVNG
CHHHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEECC
LNYTDRATGESHHVALAGIFVQIGLVPNTEWLKGVVELTPRGEIIVDARGQTSIPGVFAA
CCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHEECCCCCEEEECCCCCCCCCEEEE
GDVTDTPFKQIIIAMGSGASASLGAFDYLIRHSDEEIAETKTTDTKAA
CCCCCCCHHEEEEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLAQDIVSLSSLVSLKETQGERT
CCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAMTVINPQLNTQISFAGLPMGHEFTSLVLALLHTGGHPIKLSDDIIEQIRNLPGKYAFE
CEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHCCCCCEEEE
TYVSLTCQNCPDVVQALNMMAAINPNITNVMIDGALFQDEVANRNIMAVPSVYLNGEVFA
HEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCEEEECEEEECCEEEE
AGRISIGEILNKLDTNAASRKADEINEKAPFEVLVVGGGPAGAAAAIYAARKGLRTGIVA
ECCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVSLSTDGLFELE
HHCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHCEEEEEEEECCCEEEEEECCCEEEEE
LASGAKLRSRTVLLATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNS
ECCCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDKTLRADDVLQRKAASMGNIKIITQAMTTEVTGDGTRVNG
CHHHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEECC
LNYTDRATGESHHVALAGIFVQIGLVPNTEWLKGVVELTPRGEIIVDARGQTSIPGVFAA
CCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHEECCCCCEEEECCCCCCCCCEEEE
GDVTDTPFKQIIIAMGSGASASLGAFDYLIRHSDEEIAETKTTDTKAA
CCCCCCCHHEEEEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]