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The map label for this gene is rumB

Identifier: 160876725

GI number: 160876725

Start: 4267729

End: 4268901

Strand: Reverse

Name: rumB

Synonym: Sbal195_3619

Alternate gene names: 160876725

Gene position: 4268901-4267729 (Counterclockwise)

Preceding gene: 160876726

Following gene: 160876724

Centisome position: 79.83

GC content: 48.34

Gene sequence:

>1173_bases
GTGAGTGCTGCTATTGTGAATGCCGTTAAGCAATGTGGTTATTTTAATCAAGGTCAGTGCCTTTCTTGCCGGCATATTCA
GCAGCCTCTAGCGCAGCAAGTAGCGGTTAAAACCCAAACGTTACTGCAATTACTCGCGCCTTTTATTCCCGCAAATTCGG
CAGAACTTTTTCTGCCTCCCATTACTGGCGATGACAGCGGCTTTCGTAATAAAGCCAAAATGGTAGTACTCGGCGCCGCC
CATGAACCCGTGTTGGGGATTGTGAGCCCAAGTGGCGAGGCGGTGGATCTTTGCGACTGCCTGTTATATCCAGCTGATAT
GCAAGCCTTGTTGCACCGTTTAACCCGCTTTGTGCAGCAGGCGGGCCTTCCTCCCTATCGGGTTGATAAAGCCAAGGGCG
AGCTTAAGTTTATTCTGCTGACCCGCAGCCAAGTACGCGGCGAATATCTGCTGCGTTTTGTGCTGCGATCACACAATGGC
ATTGAACGTATCGAACGCGAATTACCTGCATTACTGGCCGAATATCCGCAAATTAAAGTGGTGTCAGTCAATATCCAACC
AGTTCATATGGCAATCCTTGAGGGCGATGAAGAGATTTTTTTAACTGAGAATACTAGGCTTGAAGAACGTTTTAATCATG
TGCCTTTGTTCATTCGGCCCAAGAGCTTTTTCCAGACCAATCCGCAGGTTGCGGCTCAGTTATATCAAACCGCCCGTGAG
TGGGTTGCCGAGTTTTCTCCTCGCTCGTTATGGGATCTGTTTTGTGGCGTCGGCGGTTTCGGACTACACTGCGCATCCAA
AGACATAACACTCACAGGGATTGAAATTGAAGCCGAGGCGATTGCCTGCGCGCAAATGTCAGCGCAAATGATGGGGCTCG
AAAACGTGCAATTTATGGCGCTCGATTCCACCGACTTTGCCAAGGGCAAAAGCGCTGCCGATAAGCCGGATTTGATCATT
GTTAATCCACCAAGGCGCGGCATTGGCGAGGCTTTATGCCAGTCATTAAGCGAGTTTGCACCTAAGGCAATTCTTTATTC
CAGCTGTAATCCCAAAACCTTAGCGAAAGATCTCGAGCATATTCAAGGTTATCACTTAACCAAGGTGCAGCTATTTGATT
TATTCCCCCACACGGATCATTTTGAAGTCTTAGCTATGTTGGTGAAGGATTAA

Upstream 100 bases:

>100_bases
TAAAATTGCGCCCTTGGTACTGGTTGAGTGTGTTTCGAGATGATTTTTACCCGTAAAACCGCTACAGTGTGGCGCTTTTT
TACTGAATCTAGGATCTGTC

Downstream 100 bases:

>100_bases
TCTTTTAGCTCATCAATGTATCTCTAAGCATTTGTACTCACTCGTTTTACGACTCTTGAAGTCCGCTCAAAAAATCTTAA
GGGCTCTGCAGTGTGGAGCC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLLQLLAPFIPANSAELFLPPITGDDSGFRNKAKMVVLGAA
HEPVLGIVSPSGEAVDLCDCLLYPADMQALLHRLTRFVQQAGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNG
IERIERELPALLAEYPQIKVVSVNIQPVHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
WVAEFSPRSLWDLFCGVGGFGLHCASKDITLTGIEIEAEAIACAQMSAQMMGLENVQFMALDSTDFAKGKSAADKPDLII
VNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHTDHFEVLAMLVKD

Sequences:

>Translated_390_residues
MSAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLLQLLAPFIPANSAELFLPPITGDDSGFRNKAKMVVLGAA
HEPVLGIVSPSGEAVDLCDCLLYPADMQALLHRLTRFVQQAGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNG
IERIERELPALLAEYPQIKVVSVNIQPVHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
WVAEFSPRSLWDLFCGVGGFGLHCASKDITLTGIEIEAEAIACAQMSAQMMGLENVQFMALDSTDFAKGKSAADKPDLII
VNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHTDHFEVLAMLVKD
>Mature_389_residues
SAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLLQLLAPFIPANSAELFLPPITGDDSGFRNKAKMVVLGAAH
EPVLGIVSPSGEAVDLCDCLLYPADMQALLHRLTRFVQQAGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNGI
ERIERELPALLAEYPQIKVVSVNIQPVHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTAREW
VAEFSPRSLWDLFCGVGGFGLHCASKDITLTGIEIEAEAIACAQMSAQMMGLENVQFMALDSTDFAKGKSAADKPDLIIV
NPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHTDHFEVLAMLVKD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI51173878, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI269784680, Length=190, Percent_Identity=29.4736842105263, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI269784676, Length=190, Percent_Identity=29.4736842105263, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI20149688, Length=190, Percent_Identity=29.4736842105263, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI269784678, Length=190, Percent_Identity=29.4736842105263, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=54.8812664907652, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789148, Length=181, Percent_Identity=31.4917127071823, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24666579, Length=362, Percent_Identity=25.1381215469613, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMB_SHEB2 (B8E5Q5)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002359331.1
- ProteinModelPortal:   B8E5Q5
- GeneID:   7086038
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_3424
- HOGENOM:   HBG296593
- OMA:   SSCNPHT
- ProtClustDB:   PRK03522
- HAMAP:   MF_01012
- InterPro:   IPR011825
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02085

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.189

Molecular weight: Translated: 43330; Mature: 43199

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 349-349 BINDING 225-225 BINDING 254-254 BINDING 275-275 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLLQLLAPFIPANSAELFLPP
CCHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
ITGDDSGFRNKAKMVVLGAAHEPVLGIVSPSGEAVDLCDCLLYPADMQALLHRLTRFVQQ
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNGIERIERELPALLAEYPQIKV
CCCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VSVNIQPVHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
EEEECCEEEEEEEECCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHH
WVAEFSPRSLWDLFCGVGGFGLHCASKDITLTGIEIEAEAIACAQMSAQMMGLENVQFMA
HHHHCCCHHHHHHHHCCCCCCEEECCCCEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEE
LDSTDFAKGKSAADKPDLIIVNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEH
ECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
IQGYHLTKVQLFDLFPHTDHFEVLAMLVKD
HCCCCEEEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLLQLLAPFIPANSAELFLPP
CHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
ITGDDSGFRNKAKMVVLGAAHEPVLGIVSPSGEAVDLCDCLLYPADMQALLHRLTRFVQQ
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNGIERIERELPALLAEYPQIKV
CCCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VSVNIQPVHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
EEEECCEEEEEEEECCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHH
WVAEFSPRSLWDLFCGVGGFGLHCASKDITLTGIEIEAEAIACAQMSAQMMGLENVQFMA
HHHHCCCHHHHHHHHCCCCCCEEECCCCEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEE
LDSTDFAKGKSAADKPDLIIVNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEH
ECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
IQGYHLTKVQLFDLFPHTDHFEVLAMLVKD
HCCCCEEEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA