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The map label for this gene is def2 [H]

Identifier: 160876705

GI number: 160876705

Start: 4234409

End: 4234966

Strand: Direct

Name: def2 [H]

Synonym: Sbal195_3599

Alternate gene names: 160876705

Gene position: 4234409-4234966 (Clockwise)

Preceding gene: 160876704

Following gene: 160876706

Centisome position: 79.19

GC content: 48.92

Gene sequence:

>558_bases
ATGTTTAAACATTTGATGCCAAAACAAACCCCGCCTTTATTACCCATAGCGTTAGTGGGTGAAGCTATCCTAGGGCAGAC
TGCAATTGCAGTGCATCATTTCGATGCCGAGCTCGCGCACCTTGCAGAGCAAATGTCTGCCAGCATGGAAGCCGCCAAAG
GCGTTGGCATCGCGGCGCCACAGGTTCACAGTCCCTTAGCTTTATTTATTATGGCTTCTCGACCTAATGAACGTTATCCC
GACGCACCCAATATGGCGCCTGTAGTCGTGGTTAATCCACAAATCATTCATGCATCGAGTGAATTAGTCGGCGGCGAAGA
AGGTTGCCTGTCCGTTCCCGGCCAAAGATTTAACATACTAAGACATCAAGCCATTGAAGTGCGCTACCAGAATTTACAAG
GCGAGTGGCAACAGGCCGAATTAACAGGCTTTATTGCGCGGATTTTTCAACACGAATTTGATCACCTGCAAGGGATCACC
TTACTCGAGCGTTCACGTATGCCTGAACAGGCTCAGTCGATTTCAGCCCAGTCCATCGAACAAGGTGAAGCGCAATAA

Upstream 100 bases:

>100_bases
ACTCAGGCTGAAGAAACGCCGCCGCCACATTACTAATACTGACGCTTTAAAATAAGACTTTGATTCAAAGACGGTTACCG
CAAAATATCCAGGGAATGCG

Downstream 100 bases:

>100_bases
TGAAACTGTCTTTGGTTACACCTTTATCTCGCGCGTTTATCGCCCCCGCTTTGGCAATAGGATTGGCGCTAGGTTTATCG
GGCTGCGCCTACAACAGTAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MFKHLMPKQTPPLLPIALVGEAILGQTAIAVHHFDAELAHLAEQMSASMEAAKGVGIAAPQVHSPLALFIMASRPNERYP
DAPNMAPVVVVNPQIIHASSELVGGEEGCLSVPGQRFNILRHQAIEVRYQNLQGEWQQAELTGFIARIFQHEFDHLQGIT
LLERSRMPEQAQSISAQSIEQGEAQ

Sequences:

>Translated_185_residues
MFKHLMPKQTPPLLPIALVGEAILGQTAIAVHHFDAELAHLAEQMSASMEAAKGVGIAAPQVHSPLALFIMASRPNERYP
DAPNMAPVVVVNPQIIHASSELVGGEEGCLSVPGQRFNILRHQAIEVRYQNLQGEWQQAELTGFIARIFQHEFDHLQGIT
LLERSRMPEQAQSISAQSIEQGEAQ
>Mature_185_residues
MFKHLMPKQTPPLLPIALVGEAILGQTAIAVHHFDAELAHLAEQMSASMEAAKGVGIAAPQVHSPLALFIMASRPNERYP
DAPNMAPVVVVNPQIIHASSELVGGEEGCLSVPGQRFNILRHQAIEVRYQNLQGEWQQAELTGFIARIFQHEFDHLQGIT
LLERSRMPEQAQSISAQSIEQGEAQ

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=166, Percent_Identity=32.5301204819277, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1789682, Length=137, Percent_Identity=32.8467153284672, Blast_Score=76, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20282; Mature: 20282

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHLMPKQTPPLLPIALVGEAILGQTAIAVHHFDAELAHLAEQMSASMEAAKGVGIAAP
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QVHSPLALFIMASRPNERYPDAPNMAPVVVVNPQIIHASSELVGGEEGCLSVPGQRFNIL
CCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCEEEECCHHHCCCCCCCCCCCCHHHHHH
RHQAIEVRYQNLQGEWQQAELTGFIARIFQHEFDHLQGITLLERSRMPEQAQSISAQSIE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
QGEAQ
CCCCC
>Mature Secondary Structure
MFKHLMPKQTPPLLPIALVGEAILGQTAIAVHHFDAELAHLAEQMSASMEAAKGVGIAAP
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QVHSPLALFIMASRPNERYPDAPNMAPVVVVNPQIIHASSELVGGEEGCLSVPGQRFNIL
CCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCEEEECCHHHCCCCCCCCCCCCHHHHHH
RHQAIEVRYQNLQGEWQQAELTGFIARIFQHEFDHLQGITLLERSRMPEQAQSISAQSIE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
QGEAQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]