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The map label for this gene is oprF [H]

Identifier: 160876689

GI number: 160876689

Start: 4214366

End: 4215823

Strand: Reverse

Name: oprF [H]

Synonym: Sbal195_3583

Alternate gene names: 160876689

Gene position: 4215823-4214366 (Counterclockwise)

Preceding gene: 160876690

Following gene: 160876686

Centisome position: 78.84

GC content: 48.97

Gene sequence:

>1458_bases
ATGGGACATAAGACGTTATTGAGTGCTGTACTTTTGACCGCGACCTGCGGTGTCGCCACTTTTGCGGTGAATGCTGAATC
GCTGTTTACTCCCTTTCCTGATGCCAAATCGCCTGAAGAAAAGCGCATCCACTTTACGCCCTTTGAACTTATCACCGCAG
TGAATGGTGACAAGTTCACTCTGTTGCCTGTATCAGGAGAATTGACTCGACAAAGCGTTGAGTTACCCGAGGGGTATAGT
CCTGAGCATGTTATCAATAATTATTTGGCACAGCTAAAAAAGCTGAATGCTGAAGTGTTATTTAGTTGTCAGGCCGTCAC
CTGCGGTGATGGTCAAGCCATGCAAACTCAGCTAAAGCCGCTTAAAAGCGTGAATGCCGACTATCACAGTGCTTACGTGG
CGGCAAAACTGAAAGGTACTCGCGGTGAGGTTTACACCAGTATCTATGTCGTCAATCGCGAGGATAGATACACCTATCTG
CAAGTCGATATTCTCGACGCTATCCCTGAGCCGCTAGACTTGATCCAAGCCAATAGTGACTTTTTACAGCGGGCACCGAA
AGAGGTTGAGATTAAAGATAGGCGTAGTGACGATGCTCAAGGAGCGGCAGATCATCCTTTGCTGGGGCGTCTGCCTGGCA
GTTACATTGATCGCTATAAGCAAACCAATTTTACGCAGGTGCCTGTGATTGTGGGCATTGCCGGAGCTGACTACCAGACC
AAGTCGCTGGATGCCAAACTCACCCAGATAATCTACCGCATGCCTGAAAGCTATTCCCTGTTTGAAATCAACAGCAATTA
TGCCGCCGCAGCACAAAAGCTGGCAGCAGAGCGGGTATTCCATTGCAAAGGCACGGCTTGCGGCGACGATGATAGCCTGA
TTAATGCCATCAAATTAATCAAGGACGATAAAGAGGACGAATGGCAGGAGTATCAGCTGTTTAAGCTTAGTCACGCAAAG
GGCGATGTTTACTTCGATATCTATTCCCAAGGATATTTCGATGGCACACCTGCCGATACTACGCTTAGGGTAATGGAGCT
TTCTACTCTGAAGGATGATCGTGTGGCGATTAATCTCGATGCCTTGACTGATGCGATTAGCCAAACTGGCAAGGCGACGT
TGGAGGGATTGCTGTTTGATTATGACAGTGAGCGTATGTTGCCTGAGTCCAAACCTGTACTCGACGTACTCGCAAGCTAT
CTTAAGCAGCATCCGGCACTGAGTTTCTATGTGGTCGGACATACCGATGACAAGGGCGAGCGGAGTTACAATCAAAGCCT
GTCGGAGCGGCGCGCCGCAGCTGTGATTAAGCAGTTGAGTGACGGGTTTCAAATTCCGTCGGCCCAATTAACCGCCCACG
GTAACGGGGAGTACAGCCCAGTGGCCAGCAACACTAATGACGCCGGACAAAAATTGAATCGCAGAGTGGAGTTAGTGCTT
CGTTCGGATAAAAAGTAG

Upstream 100 bases:

>100_bases
AATACCTAGGTAAAAATTTTTATCGTATTATCCCATCACTATTTCTTTATCTATCTAGTTGGTTCGCGAGAGCTAGATTC
CCCACAGAAAAGGATTTTTT

Downstream 100 bases:

>100_bases
CTCAAGGTTATCAGCTTGAAAGGCTATCTTCGTTCCATCAAAAACGCCTGAGATGACTCGGGCGTTTCTAGCTTCAGAGG
CGTTCATTGGAAAATATTGT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGHKTLLSAVLLTATCGVATFAVNAESLFTPFPDAKSPEEKRIHFTPFELITAVNGDKFTLLPVSGELTRQSVELPEGYS
PEHVINNYLAQLKKLNAEVLFSCQAVTCGDGQAMQTQLKPLKSVNADYHSAYVAAKLKGTRGEVYTSIYVVNREDRYTYL
QVDILDAIPEPLDLIQANSDFLQRAPKEVEIKDRRSDDAQGAADHPLLGRLPGSYIDRYKQTNFTQVPVIVGIAGADYQT
KSLDAKLTQIIYRMPESYSLFEINSNYAAAAQKLAAERVFHCKGTACGDDDSLINAIKLIKDDKEDEWQEYQLFKLSHAK
GDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLDALTDAISQTGKATLEGLLFDYDSERMLPESKPVLDVLASY
LKQHPALSFYVVGHTDDKGERSYNQSLSERRAAAVIKQLSDGFQIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
RSDKK

Sequences:

>Translated_485_residues
MGHKTLLSAVLLTATCGVATFAVNAESLFTPFPDAKSPEEKRIHFTPFELITAVNGDKFTLLPVSGELTRQSVELPEGYS
PEHVINNYLAQLKKLNAEVLFSCQAVTCGDGQAMQTQLKPLKSVNADYHSAYVAAKLKGTRGEVYTSIYVVNREDRYTYL
QVDILDAIPEPLDLIQANSDFLQRAPKEVEIKDRRSDDAQGAADHPLLGRLPGSYIDRYKQTNFTQVPVIVGIAGADYQT
KSLDAKLTQIIYRMPESYSLFEINSNYAAAAQKLAAERVFHCKGTACGDDDSLINAIKLIKDDKEDEWQEYQLFKLSHAK
GDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLDALTDAISQTGKATLEGLLFDYDSERMLPESKPVLDVLASY
LKQHPALSFYVVGHTDDKGERSYNQSLSERRAAAVIKQLSDGFQIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
RSDKK
>Mature_484_residues
GHKTLLSAVLLTATCGVATFAVNAESLFTPFPDAKSPEEKRIHFTPFELITAVNGDKFTLLPVSGELTRQSVELPEGYSP
EHVINNYLAQLKKLNAEVLFSCQAVTCGDGQAMQTQLKPLKSVNADYHSAYVAAKLKGTRGEVYTSIYVVNREDRYTYLQ
VDILDAIPEPLDLIQANSDFLQRAPKEVEIKDRRSDDAQGAADHPLLGRLPGSYIDRYKQTNFTQVPVIVGIAGADYQTK
SLDAKLTQIIYRMPESYSLFEINSNYAAAAQKLAAERVFHCKGTACGDDDSLINAIKLIKDDKEDEWQEYQLFKLSHAKG
DVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLDALTDAISQTGKATLEGLLFDYDSERMLPESKPVLDVLASYL
KQHPALSFYVVGHTDDKGERSYNQSLSERRAAAVIKQLSDGFQIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVLR
SDKK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=106, Percent_Identity=37.7358490566038, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 53762; Mature: 53631

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHKTLLSAVLLTATCGVATFAVNAESLFTPFPDAKSPEEKRIHFTPFELITAVNGDKFT
CCHHHHHHHHHHHHHCCHHEEEECHHHHCCCCCCCCCCCCCEEEECHHHHHEECCCCEEE
LLPVSGELTRQSVELPEGYSPEHVINNYLAQLKKLNAEVLFSCQAVTCGDGQAMQTQLKP
EEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCHHHHHHHHH
LKSVNADYHSAYVAAKLKGTRGEVYTSIYVVNREDRYTYLQVDILDAIPEPLDLIQANSD
HHHCCCCHHHEEEEEEECCCCCEEEEEEEEEECCCCEEEEEEEEHHHCCCCHHHHCCCCH
FLQRAPKEVEIKDRRSDDAQGAADHPLLGRLPGSYIDRYKQTNFTQVPVIVGIAGADYQT
HHHCCCCCCEECCCCCCCCCCCCCCCCHHCCCHHHHHHHHCCCCEECCEEEEEECCCCCC
KSLDAKLTQIIYRMPESYSLFEINSNYAAAAQKLAAERVFHCKGTACGDDDSLINAIKLI
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHH
KDDKEDEWQEYQLFKLSHAKGDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLD
HCCCCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEHH
ALTDAISQTGKATLEGLLFDYDSERMLPESKPVLDVLASYLKQHPALSFYVVGHTDDKGE
HHHHHHHHCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCH
RSYNQSLSERRAAAVIKQLSDGFQIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEE
RSDKK
ECCCC
>Mature Secondary Structure 
GHKTLLSAVLLTATCGVATFAVNAESLFTPFPDAKSPEEKRIHFTPFELITAVNGDKFT
CHHHHHHHHHHHHHCCHHEEEECHHHHCCCCCCCCCCCCCEEEECHHHHHEECCCCEEE
LLPVSGELTRQSVELPEGYSPEHVINNYLAQLKKLNAEVLFSCQAVTCGDGQAMQTQLKP
EEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCHHHHHHHHH
LKSVNADYHSAYVAAKLKGTRGEVYTSIYVVNREDRYTYLQVDILDAIPEPLDLIQANSD
HHHCCCCHHHEEEEEEECCCCCEEEEEEEEEECCCCEEEEEEEEHHHCCCCHHHHCCCCH
FLQRAPKEVEIKDRRSDDAQGAADHPLLGRLPGSYIDRYKQTNFTQVPVIVGIAGADYQT
HHHCCCCCCEECCCCCCCCCCCCCCCCHHCCCHHHHHHHHCCCCEECCEEEEEECCCCCC
KSLDAKLTQIIYRMPESYSLFEINSNYAAAAQKLAAERVFHCKGTACGDDDSLINAIKLI
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHH
KDDKEDEWQEYQLFKLSHAKGDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLD
HCCCCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEHH
ALTDAISQTGKATLEGLLFDYDSERMLPESKPVLDVLASYLKQHPALSFYVVGHTDDKGE
HHHHHHHHCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCH
RSYNQSLSERRAAAVIKQLSDGFQIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEE
RSDKK
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]