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The map label for this gene is rluF [H]

Identifier: 160876688

GI number: 160876688

Start: 4213492

End: 4214202

Strand: Direct

Name: rluF [H]

Synonym: Sbal195_3582

Alternate gene names: 160876688

Gene position: 4213492-4214202 (Clockwise)

Preceding gene: 160876687

Following gene: 160876692

Centisome position: 78.8

GC content: 51.9

Gene sequence:

>711_bases
ATGCGCCTTGCAAAATACCTCGCCCAGTGTGGCATAGGCTCACGCCGTGAAGCCTGCCGTTTGATTGAGGCCGGACGCAT
TACACTCAATGGCCATATCGCCCGCCACACAGATCCCATCCGTTTGGACGAATTATTAAACTGCCTCGACCGTATTGAGC
TTGATGGCGCACCTATCACTCAAACCGAAACCCTCGCCTACTGGGTATTTAACAAAGCCGTAGGCACGGATTGCCGCCTG
CTTGAGCAGGATGAAACGAGTCTTGTACACTTGCTTCCCAAAGCGCCAAGACTCTATCCCGTCGGCAGGCTCGATAAGGA
TTCCCGCGGCCTGTTGCTGTTAACTAACGATGGTGAGCTGACCCATAAACTGATGCACCCCTGTTTTGCCCACAGCAAAA
CCTACCATGTGCGCCTCGATAGGCCATTCACTGATGAGTTTGTTGAACAAATGGCCAGTGGAGTGCGTTATAAAGATGTG
CAAACCCTGCCCTGCCAAGTCCGCCGCCTCAGTTCGGACAGCTTTGAAATCGTGCTGACTCAGGGACTAAATCGCCAAAT
TCGGCGTATGGCCATCGCCTTAGGCTATAAGGTAATTGACTTACAGCGCGTCGCATTAATGACATTAACGCTAGAATCCT
TAGCCGAAGGAGCGATGCGGCCACTCACCCCACAGGAAGTTGCAGTGCTTAAAGCCACAGTCACAATTTAA

Upstream 100 bases:

>100_bases
ACAGCAGGTTGAAATAGCTAATTAAGATGCTGATAAGTACAATTGAAGGAGCCGAATTCGGCTCCTTTTTTATGTTTAAA
TTCATCAACCTTGGATTGAT

Downstream 100 bases:

>100_bases
TGGCAGACAAATCGCGAATGCCCTCAAGCAGCTAGCCTCAATTTGAGACAAATGCCCGCGCGAACAATATTTTCCAATGA
ACGCCTCTGAAGCTAGAAAC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRLAKYLAQCGIGSRREACRLIEAGRITLNGHIARHTDPIRLDELLNCLDRIELDGAPITQTETLAYWVFNKAVGTDCRL
LEQDETSLVHLLPKAPRLYPVGRLDKDSRGLLLLTNDGELTHKLMHPCFAHSKTYHVRLDRPFTDEFVEQMASGVRYKDV
QTLPCQVRRLSSDSFEIVLTQGLNRQIRRMAIALGYKVIDLQRVALMTLTLESLAEGAMRPLTPQEVAVLKATVTI

Sequences:

>Translated_236_residues
MRLAKYLAQCGIGSRREACRLIEAGRITLNGHIARHTDPIRLDELLNCLDRIELDGAPITQTETLAYWVFNKAVGTDCRL
LEQDETSLVHLLPKAPRLYPVGRLDKDSRGLLLLTNDGELTHKLMHPCFAHSKTYHVRLDRPFTDEFVEQMASGVRYKDV
QTLPCQVRRLSSDSFEIVLTQGLNRQIRRMAIALGYKVIDLQRVALMTLTLESLAEGAMRPLTPQEVAVLKATVTI
>Mature_236_residues
MRLAKYLAQCGIGSRREACRLIEAGRITLNGHIARHTDPIRLDELLNCLDRIELDGAPITQTETLAYWVFNKAVGTDCRL
LEQDETSLVHLLPKAPRLYPVGRLDKDSRGLLLLTNDGELTHKLMHPCFAHSKTYHVRLDRPFTDEFVEQMASGVRYKDV
QTLPCQVRRLSSDSFEIVLTQGLNRQIRRMAIALGYKVIDLQRVALMTLTLESLAEGAMRPLTPQEVAVLKATVTI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=229, Percent_Identity=37.9912663755458, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=33.9055793991416, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787524, Length=224, Percent_Identity=33.9285714285714, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081838, Length=145, Percent_Identity=32.4137931034483, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26660; Mature: 26660

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAKYLAQCGIGSRREACRLIEAGRITLNGHIARHTDPIRLDELLNCLDRIELDGAPIT
CCHHHHHHHCCCCCHHHHHHHHHCCEEEEECEEECCCCCCCHHHHHHHHHHHCCCCCCCC
QTETLAYWVFNKAVGTDCRLLEQDETSLVHLLPKAPRLYPVGRLDKDSRGLLLLTNDGEL
CHHHEEEEEEHHHCCCCCEEECCCCCCEEEECCCCCCEEECCCCCCCCCCEEEEECCCHH
THKLMHPCFAHSKTYHVRLDRPFTDEFVEQMASGVRYKDVQTLPCQVRRLSSDSFEIVLT
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEECCCCCCCHHHHHCCCCCEEEEEE
QGLNRQIRRMAIALGYKVIDLQRVALMTLTLESLAEGAMRPLTPQEVAVLKATVTI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECC
>Mature Secondary Structure
MRLAKYLAQCGIGSRREACRLIEAGRITLNGHIARHTDPIRLDELLNCLDRIELDGAPIT
CCHHHHHHHCCCCCHHHHHHHHHCCEEEEECEEECCCCCCCHHHHHHHHHHHCCCCCCCC
QTETLAYWVFNKAVGTDCRLLEQDETSLVHLLPKAPRLYPVGRLDKDSRGLLLLTNDGEL
CHHHEEEEEEHHHCCCCCEEECCCCCCEEEECCCCCCEEECCCCCCCCCCEEEEECCCHH
THKLMHPCFAHSKTYHVRLDRPFTDEFVEQMASGVRYKDVQTLPCQVRRLSSDSFEIVLT
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEECCCCCCCHHHHHCCCCCEEEEEE
QGLNRQIRRMAIALGYKVIDLQRVALMTLTLESLAEGAMRPLTPQEVAVLKATVTI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]