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The map label for this gene is dacA [H]

Identifier: 160876563

GI number: 160876563

Start: 4077198

End: 4078373

Strand: Direct

Name: dacA [H]

Synonym: Sbal195_3457

Alternate gene names: 160876563

Gene position: 4077198-4078373 (Clockwise)

Preceding gene: 160876562

Following gene: 160876564

Centisome position: 76.25

GC content: 44.81

Gene sequence:

>1176_bases
ATGATGAATTTTGTAAAAAGTCCCATTAAAACATTGCTGCTAATTTCCAGTGCCTCTCTCCCCGTTTATGCTGCGCAGCC
TATTATTACACCTGATGCACCTTCAGTAGCCGCCAAGGCGTTCGTATTGATGGATTATAATTCGGGTCAGATTATCGCTG
AAAGTAACGCTTATGAAAGCCTTAACCCAGCCAGTTTAACTAAGATGATGACCAGTTACGTGATCGGTCAAGAAATCAAA
GCGGGCAATGTCTCCGTTGATGATGATGTGACTATCAGTAAAAATGCTTGGTCTAAAAACTTCTCAGACTCTTCTAAGAT
GTTCATTGAAGTAGGCACTACGGTTAAAGTGGGCGATCTAAACCGCGGTATCATCATTCAATCGGGTAACGATGCGTGTG
TGGCGATGGCCGAGCACATTGCCGGTACTGAAGGCGCCTTCGTTGACATGATGAACTCATGGGCGAAGCAACTGGGGATG
CGTGATAGCTACTTCGAAAACGCCCACGGTTTAGATGCAGAAAACCACAAATCAACCGCCTATGATATGGCATTACTGGG
CGCAGCGTTAATCCGCGACGTGCCAGATGAATACCACATCTATAGCGAAAAATTCTATACATTCAATGGTATCAAACAAT
ACAACCGTAATGGTTTATTGTGGGATAACAGCATGAACGTGGACGGTATCAAAACCGGTCACACCTCAGGTGCGGGCTAC
AACTTAGTGGCATCGGCAACCAAAGACGGCATGCGTTTAATTTCAGTGGTGATGGGCACTCAAAGTGAATCAGCCCGTAA
AGCTGAAAGTAAGAAATTACTGACCTATGGCTTCCGTTTCTTCGAAACAGTAACACCATACAAGGCGGGTGATAGCTTTA
TCACTCAGCAAATTTGGTACGGTGACAAGAGCACGGTTGATTTAGGCGTGGCAACTGACACACCTATCACCATCAGCCGT
GGCCGTGGTAAAGACTTGAAAGCAAACTTCGAGCTGACTAAACCATTAGATGCACCGTTGAAAAAAGGTGAAACTGTTGG
CCGTCTATTCTTCCAACTTGATGGTAAAGACATTGCCCAGTTCCCACTGGTGACGTTGCAAGAAGTCAACGAAGGCAGCT
GGTTTAGTAAATTAGTTGATTACTTTAAGCAGATGTTTTCTGGATGGTTTAGCTAA

Upstream 100 bases:

>100_bases
GAAGGAACCTTCACTAAAACCCACTGTCGGAAAGTGGTACACTGTTGAAGATCTTCTCGAATCCTTTCCATTTCATACAA
CTAAAAGACGTGCCAAGTTA

Downstream 100 bases:

>100_bases
GCTAAGTCACATAAATAAAGCCACCTTCGGGTGGCTTTATTTTTTTGGGGAGTGCATCGCCCTTGAATCGGGTATAATCG
TCACCATATATATCCCAATA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MMNFVKSPIKTLLLISSASLPVYAAQPIITPDAPSVAAKAFVLMDYNSGQIIAESNAYESLNPASLTKMMTSYVIGQEIK
AGNVSVDDDVTISKNAWSKNFSDSSKMFIEVGTTVKVGDLNRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGM
RDSYFENAHGLDAENHKSTAYDMALLGAALIRDVPDEYHIYSEKFYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSESARKAESKKLLTYGFRFFETVTPYKAGDSFITQQIWYGDKSTVDLGVATDTPITISR
GRGKDLKANFELTKPLDAPLKKGETVGRLFFQLDGKDIAQFPLVTLQEVNEGSWFSKLVDYFKQMFSGWFS

Sequences:

>Translated_391_residues
MMNFVKSPIKTLLLISSASLPVYAAQPIITPDAPSVAAKAFVLMDYNSGQIIAESNAYESLNPASLTKMMTSYVIGQEIK
AGNVSVDDDVTISKNAWSKNFSDSSKMFIEVGTTVKVGDLNRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGM
RDSYFENAHGLDAENHKSTAYDMALLGAALIRDVPDEYHIYSEKFYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSESARKAESKKLLTYGFRFFETVTPYKAGDSFITQQIWYGDKSTVDLGVATDTPITISR
GRGKDLKANFELTKPLDAPLKKGETVGRLFFQLDGKDIAQFPLVTLQEVNEGSWFSKLVDYFKQMFSGWFS
>Mature_391_residues
MMNFVKSPIKTLLLISSASLPVYAAQPIITPDAPSVAAKAFVLMDYNSGQIIAESNAYESLNPASLTKMMTSYVIGQEIK
AGNVSVDDDVTISKNAWSKNFSDSSKMFIEVGTTVKVGDLNRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGM
RDSYFENAHGLDAENHKSTAYDMALLGAALIRDVPDEYHIYSEKFYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSESARKAESKKLLTYGFRFFETVTPYKAGDSFITQQIWYGDKSTVDLGVATDTPITISR
GRGKDLKANFELTKPLDAPLKKGETVGRLFFQLDGKDIAQFPLVTLQEVNEGSWFSKLVDYFKQMFSGWFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=366, Percent_Identity=57.6502732240437, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787062, Length=370, Percent_Identity=55.1351351351351, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI87082022, Length=382, Percent_Identity=47.3821989528796, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI87082054, Length=251, Percent_Identity=27.0916334661355, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42971; Mature: 42971

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNFVKSPIKTLLLISSASLPVYAAQPIITPDAPSVAAKAFVLMDYNSGQIIAESNAYES
CCCHHHHHHHHEEEEECCCCCEEECCCEECCCCCCHHEEEEEEEECCCCEEEECCCCCCC
LNPASLTKMMTSYVIGQEIKAGNVSVDDDVTISKNAWSKNFSDSSKMFIEVGTTVKVGDL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEECCC
NRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGMRDSYFENAHGLDAENHKSTA
CCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHH
YDMALLGAALIRDVPDEYHIYSEKFYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
HHHHHHHHHHHHHCCHHHEEECCEEEEECCHHHHCCCCEEECCCCCCCCEECCCCCCCCC
NLVASATKDGMRLISVVMGTQSESARKAESKKLLTYGFRFFETVTPYKAGDSFITQQIWY
EEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEC
GDKSTVDLGVATDTPITISRGRGKDLKANFELTKPLDAPLKKGETVGRLFFQLDGKDIAQ
CCCCEEEEEEECCCCEEEECCCCCCEEECEEECCCCCCHHHCCCCEEEEEEEECCCCHHH
FPLVTLQEVNEGSWFSKLVDYFKQMFSGWFS
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMNFVKSPIKTLLLISSASLPVYAAQPIITPDAPSVAAKAFVLMDYNSGQIIAESNAYES
CCCHHHHHHHHEEEEECCCCCEEECCCEECCCCCCHHEEEEEEEECCCCEEEECCCCCCC
LNPASLTKMMTSYVIGQEIKAGNVSVDDDVTISKNAWSKNFSDSSKMFIEVGTTVKVGDL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEECCC
NRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGMRDSYFENAHGLDAENHKSTA
CCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHH
YDMALLGAALIRDVPDEYHIYSEKFYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
HHHHHHHHHHHHHCCHHHEEECCEEEEECCHHHHCCCCEEECCCCCCCCEECCCCCCCCC
NLVASATKDGMRLISVVMGTQSESARKAESKKLLTYGFRFFETVTPYKAGDSFITQQIWY
EEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEC
GDKSTVDLGVATDTPITISRGRGKDLKANFELTKPLDAPLKKGETVGRLFFQLDGKDIAQ
CCCCEEEEEEECCCCEEEECCCCCCEEECEEECCCCCCHHHCCCCEEEEEEEECCCCHHH
FPLVTLQEVNEGSWFSKLVDYFKQMFSGWFS
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]