Click here to switch to the map view.

The map label for this gene is ubiF [H]

Identifier: 160876543

GI number: 160876543

Start: 4056351

End: 4057613

Strand: Reverse

Name: ubiF [H]

Synonym: Sbal195_3437

Alternate gene names: 160876543

Gene position: 4057613-4056351 (Counterclockwise)

Preceding gene: 160876552

Following gene: 160876542

Centisome position: 75.88

GC content: 48.38

Gene sequence:

>1263_bases
ATGAGCCAAATTGATGAAACAAGTGCACCGATGACATCCGATACAACTCATTCAGACGTGACTCATGTTAAAGGCACTCA
TGTTGAAGACGCTGAGTTCGATATCATTCTCGTCGGCGGCGGTATGGTCGGTGCGGCGACGGCAATTGGATTAGCCCAGC
AGGGCTTGCAAGTGGCGGTGATTGAATCATTTGCGCCAGAGGCTTATCAAGCCGAGCAGCCGTTAGATGTGCGAGTCTCG
GCCATCAGTGTGGCCTCTGAAACGCTGCTTGAACGTCTTGGCGCCTTGGACTCACTGCTGAAAATGCGCAATGTGCCTTA
TCTTGGCTTAGAAACTTGGGAGCTTGATGGCTGCATCACCCAATTCCACTGCTCGCAAATTGGTGCTAGTCATTTAGGTC
ATATCGTTGAAAACCGTTTAATCCAACTTGCCCTGTGGCAACAAATGACGCAATTGGCTGGCATTACTTTATATTGCCCC
GATAAAGTGGTGGCATTTGCCCGTGATAGTGATGGCGTCAGTGTGCACTTACAATCGAATAGTAAACTCAACGCTAAGTT
ATTGGTCGGTGCCGATGGTGCACACTCGCAGGTACGCCAATGGGCTGGGATTGGTATTTCAGGGTGGGATTATGCTCAAT
CGGCGATGCTGATCAATATCAACACCGCACAGGGCCAGCAAGATGTCACTTGGCAGCAGTTTACGCCAAAAGGACCGCGT
TCATTATTACCTCTGCCCGGCAACAATGCTTCGCTGGTGTGGTACGACGACGCCAACCGAATAAAACAGTTAATGCAGCT
TAACAACAAACAGTTGGCCGAACAGATACGTTTGCATTTCCCTGCACGCTTAGATGCCGATTTCACCGTTGAGAATAAAG
GCAGTTTTGGTCTGACTCGTCGCCATGCACAGCGTTATTATAGTCAGAACGTGGTGATTTTAGGGGATGCCGCCCACACG
ATTAATCCGCTCGCTGGCCAAGGGGTGAACCTTGGGTTTAAGGATGTTGAAGCGTTTTTGGCGGTCATCAAGGATGCATT
AACCCAAGATAATATCTGGTGGTCAAATGAAGTGCTGGCCCAATATCAGCGCAAACGCTATCGCGATAACCAACTGATGA
TGACGGCGATGGATTTGTTTTATGCAGGTTTTAGTAACGATATCCTGCCGTTAAAGCTGCTGCGTAATGGCGCGTTAAAG
TTGGCAAACATCAATTCTCCGATCAAAAAGACCGTCCTTAAATATGCAATGGGGCTTAATTAA

Upstream 100 bases:

>100_bases
AAAATAGCTGGCGTATAGACTGGCGTATAGACTTGCGCATAGATAGTGTAAAGACGTCGCTTGGGTCGCAATAAACAAGG
TAATAAAGGTGGTAAGTAAG

Downstream 100 bases:

>100_bases
TATCGAGTTGCTCTGCGTTCAGACTCAGTGCGATGACGTGGCATTTGTTGACTTGGGCATTGAGTGCTAAAATGCCGCGT
TTTTAGCAGCTATGGTCAGT

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSQIDETSAPMTSDTTHSDVTHVKGTHVEDAEFDIILVGGGMVGAATAIGLAQQGLQVAVIESFAPEAYQAEQPLDVRVS
AISVASETLLERLGALDSLLKMRNVPYLGLETWELDGCITQFHCSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCP
DKVVAFARDSDGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVTWQQFTPKGPR
SLLPLPGNNASLVWYDDANRIKQLMQLNNKQLAEQIRLHFPARLDADFTVENKGSFGLTRRHAQRYYSQNVVILGDAAHT
INPLAGQGVNLGFKDVEAFLAVIKDALTQDNIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALK
LANINSPIKKTVLKYAMGLN

Sequences:

>Translated_420_residues
MSQIDETSAPMTSDTTHSDVTHVKGTHVEDAEFDIILVGGGMVGAATAIGLAQQGLQVAVIESFAPEAYQAEQPLDVRVS
AISVASETLLERLGALDSLLKMRNVPYLGLETWELDGCITQFHCSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCP
DKVVAFARDSDGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVTWQQFTPKGPR
SLLPLPGNNASLVWYDDANRIKQLMQLNNKQLAEQIRLHFPARLDADFTVENKGSFGLTRRHAQRYYSQNVVILGDAAHT
INPLAGQGVNLGFKDVEAFLAVIKDALTQDNIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALK
LANINSPIKKTVLKYAMGLN
>Mature_419_residues
SQIDETSAPMTSDTTHSDVTHVKGTHVEDAEFDIILVGGGMVGAATAIGLAQQGLQVAVIESFAPEAYQAEQPLDVRVSA
ISVASETLLERLGALDSLLKMRNVPYLGLETWELDGCITQFHCSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCPD
KVVAFARDSDGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVTWQQFTPKGPRS
LLPLPGNNASLVWYDDANRIKQLMQLNNKQLAEQIRLHFPARLDADFTVENKGSFGLTRRHAQRYYSQNVVILGDAAHTI
NPLAGQGVNLGFKDVEAFLAVIKDALTQDNIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALKL
ANINSPIKKTVLKYAMGLN

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=387, Percent_Identity=30.4909560723514, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI32967307, Length=328, Percent_Identity=29.5731707317073, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1786883, Length=386, Percent_Identity=48.4455958549223, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789273, Length=390, Percent_Identity=38.4615384615385, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789274, Length=402, Percent_Identity=28.1094527363184, Blast_Score=171, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI25146631, Length=434, Percent_Identity=26.7281105990783, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321694, Length=449, Percent_Identity=24.4988864142539, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920760, Length=452, Percent_Identity=27.212389380531, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 46240; Mature: 46109

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIDETSAPMTSDTTHSDVTHVKGTHVEDAEFDIILVGGGMVGAATAIGLAQQGLQVAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEE
IESFAPEAYQAEQPLDVRVSAISVASETLLERLGALDSLLKMRNVPYLGLETWELDGCIT
EHHCCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEECCCHHH
QFHCSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCPDKVVAFARDSDGVSVHLQSN
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCEEEEEECC
SKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVTWQQFTPKGPR
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCC
SLLPLPGNNASLVWYDDANRIKQLMQLNNKQLAEQIRLHFPARLDADFTVENKGSFGLTR
CCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
RHAQRYYSQNVVILGDAAHTINPLAGQGVNLGFKDVEAFLAVIKDALTQDNIWWSNEVLA
HHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALKLANINSPIKKTVLKYAMGLN
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQIDETSAPMTSDTTHSDVTHVKGTHVEDAEFDIILVGGGMVGAATAIGLAQQGLQVAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEE
IESFAPEAYQAEQPLDVRVSAISVASETLLERLGALDSLLKMRNVPYLGLETWELDGCIT
EHHCCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEECCCHHH
QFHCSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCPDKVVAFARDSDGVSVHLQSN
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCEEEEEECC
SKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVTWQQFTPKGPR
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCC
SLLPLPGNNASLVWYDDANRIKQLMQLNNKQLAEQIRLHFPARLDADFTVENKGSFGLTR
CCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
RHAQRYYSQNVVILGDAAHTINPLAGQGVNLGFKDVEAFLAVIKDALTQDNIWWSNEVLA
HHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALKLANINSPIKKTVLKYAMGLN
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]