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The map label for this gene is gspE [H]

Identifier: 160876491

GI number: 160876491

Start: 4006040

End: 4008121

Strand: Direct

Name: gspE [H]

Synonym: Sbal195_3385

Alternate gene names: 160876491

Gene position: 4006040-4008121 (Clockwise)

Preceding gene: 160876487

Following gene: 160876492

Centisome position: 74.92

GC content: 41.59

Gene sequence:

>2082_bases
ATGGTACGAACATCGACACATTGGCTTCTGGATGCTCTGCTAAGCTCAAAACCTGAGCTTGTGCTACCAGATAAATATAA
TCTTTCAATGGATACTCGTGAAGCTTGGCATCAATTACAAGAAACACTAAGGTTACCTGCTAGCGCAATTTCTGAGGCTC
TTTCTTTAGCCTATTCTACAGATGGTATTAACATACAAGAATTTAAGCCTCCCAGAGATAACCCTTTTAATGAAAGAGTT
TGTCGCGAGATAGGCATATTGCCCTTTGATTTGAGTGTTAATGAGCCTGTTATTGCAACTTATGACCCTCGTCTAACTCC
AGAGCAGCATGAACAAATACGTTTTATTCTGAGACGTGACTTTAAACTGGCTATTTTGTCTCCTGATGAAATTGATATAG
GGATTACACGACTTTTTTCGACAATTGATCACGATTATAGAACTATCGATCTGACTTCTGCGTCAGTTGAGGAAAATGAA
ACTTCTCAGTTAGCAAAAGCGATCTTACGCTCAGCTATAGATAAAAGAGCGTCGGATATTCATATGCATCCAATGGTTGG
TGGTGCAGCAATTAGGTTTCGAGTTGATGGGATTTTAGAGCGAATTGCATCGTTATCCAAGCAACAGCACGATGGCTTAT
CTCGCTTTTTTACTATGAATGCTGGCCTTGATGTGAACCCTCTAATTGCTCAAGATGGACGAATGCGATTAATTTACGGT
CAACGTGAAATTGACGTTAGGCTGTCATTATTGCCCGTATTTGATGGCATGAGAATAGTGTGTCGCCTTCTTGAGCAAGG
CAAGCAGTTTTCTTTAGTCAACAGTGGCTTTTCCCCCTCTGATTATCGTGCATTAAAACGACTTGTATCCTACGGCTCCG
GAATTGTTTTGTTAACGGGGCCTACTGGGTCTGGAAAGACATCAACACTGTACGCTCTGCTTTCAGAACTTAATAGTGTA
GATGTAAACATTATGACCTTGGAAAACCCTGTGGAATACGTTTTACCCGGTATATCACAAGTGCAAATCAATGATGCTCA
AGGTCTTTCTTTTGGTGATACGTTGCGATCAATACTAAGGCAAGACCCAGACATTGTTTTGGTAGGCGAAATACGCGATG
CGGAAACGGCAAAAATAGCATCTCAAGCGGCATTAACGGGGCATATAGTCTTGTCTACACTGCATACCAATGACGCGATG
GCGACAGTGCCTAGACTTTTGGATCTTGGGATAAATCCATCGGTTTTAGCAGATGCATTGGTTGGTGTCGTTTCACAGCG
TCTGGTAAGAAAACTATGTTCATTCTGTAAAACAAAGGTTACTAAGCCACTCAAACCTGAAGAACAGGAATATGAACGAA
TTACCGGTGAGCTGCCTAGCTGTCGTGCGCTAGGATGTGATGAGTGCCACTATACTGGCTATACGGGGCGCCTTCCTGTT
ACTGAAGTCATGGAAGTATCACCAGAGATGCGAGCGGTTATGTTGAGTGGCTGTAATAATGTTAAAGATTTAACAGAAGC
GAATAAAGGTTTTCAGTCCCAGATGGCCGTTAGCGCAGGGCAGTGGATAGTGTCTGGTGAAACAACAGCCCAAGAAATTA
GCCGTGAGTTGGGTTTGCGTTTTTGGCGTGAGCTTGCAATGGTAAATGATAAAGCACCAAATATGTTGCTCGACAATTTT
TCAGGGTCAAACCCGCTTTCAGAAGATCGCCTCAAAGTATTATTGATCTCTGATAATAAAGGGCTTCAATCAGCGCTAGC
TGAAAGTTTTCAATATGGACTAGTCTTTGTTAATAACGAACACAAAGCATCAGAAATTTTGAAAAAAGATACTTCGGTTA
TTGCTATTTTAATTGATTCTGCACTTATGCAGTCTACTCCAGCTGAATGGCTTGGAAAGCTGAGAGAGCAATTGGCTTGG
TCGGGAACCCCTGTGCTATTTTTAATCCCCCCCAATCAAAATGAAACCAAAATATTGTTGAATAAATTCCATGCTCATGC
GATGGAATACGATGAAAATATGACCCCTCCGACTGAAATTGTTAATAAGCTACAAAGCATACTGACTAAAGGAACACTCT
AA

Upstream 100 bases:

>100_bases
TTAAAATGTTTTCACCTTTTTCCTGTACGGTAAAAAGTTGTCAACATACTGCGGGCTTTATACGAAGTGATATCAAGTAA
ACAAAGGCAAAGAGGTCTTA

Downstream 100 bases:

>100_bases
TATGAACGCTCAAATATTACGAAAAAACAATAATCGAGGCTTCAACTTGTTAGAGATAATGGTTGTTGTAGCGATTATTG
GAATACTCGCGGTTGTGGCG

Product: type II secretion system protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MVRTSTHWLLDALLSSKPELVLPDKYNLSMDTREAWHQLQETLRLPASAISEALSLAYSTDGINIQEFKPPRDNPFNERV
CREIGILPFDLSVNEPVIATYDPRLTPEQHEQIRFILRRDFKLAILSPDEIDIGITRLFSTIDHDYRTIDLTSASVEENE
TSQLAKAILRSAIDKRASDIHMHPMVGGAAIRFRVDGILERIASLSKQQHDGLSRFFTMNAGLDVNPLIAQDGRMRLIYG
QREIDVRLSLLPVFDGMRIVCRLLEQGKQFSLVNSGFSPSDYRALKRLVSYGSGIVLLTGPTGSGKTSTLYALLSELNSV
DVNIMTLENPVEYVLPGISQVQINDAQGLSFGDTLRSILRQDPDIVLVGEIRDAETAKIASQAALTGHIVLSTLHTNDAM
ATVPRLLDLGINPSVLADALVGVVSQRLVRKLCSFCKTKVTKPLKPEEQEYERITGELPSCRALGCDECHYTGYTGRLPV
TEVMEVSPEMRAVMLSGCNNVKDLTEANKGFQSQMAVSAGQWIVSGETTAQEISRELGLRFWRELAMVNDKAPNMLLDNF
SGSNPLSEDRLKVLLISDNKGLQSALAESFQYGLVFVNNEHKASEILKKDTSVIAILIDSALMQSTPAEWLGKLREQLAW
SGTPVLFLIPPNQNETKILLNKFHAHAMEYDENMTPPTEIVNKLQSILTKGTL

Sequences:

>Translated_693_residues
MVRTSTHWLLDALLSSKPELVLPDKYNLSMDTREAWHQLQETLRLPASAISEALSLAYSTDGINIQEFKPPRDNPFNERV
CREIGILPFDLSVNEPVIATYDPRLTPEQHEQIRFILRRDFKLAILSPDEIDIGITRLFSTIDHDYRTIDLTSASVEENE
TSQLAKAILRSAIDKRASDIHMHPMVGGAAIRFRVDGILERIASLSKQQHDGLSRFFTMNAGLDVNPLIAQDGRMRLIYG
QREIDVRLSLLPVFDGMRIVCRLLEQGKQFSLVNSGFSPSDYRALKRLVSYGSGIVLLTGPTGSGKTSTLYALLSELNSV
DVNIMTLENPVEYVLPGISQVQINDAQGLSFGDTLRSILRQDPDIVLVGEIRDAETAKIASQAALTGHIVLSTLHTNDAM
ATVPRLLDLGINPSVLADALVGVVSQRLVRKLCSFCKTKVTKPLKPEEQEYERITGELPSCRALGCDECHYTGYTGRLPV
TEVMEVSPEMRAVMLSGCNNVKDLTEANKGFQSQMAVSAGQWIVSGETTAQEISRELGLRFWRELAMVNDKAPNMLLDNF
SGSNPLSEDRLKVLLISDNKGLQSALAESFQYGLVFVNNEHKASEILKKDTSVIAILIDSALMQSTPAEWLGKLREQLAW
SGTPVLFLIPPNQNETKILLNKFHAHAMEYDENMTPPTEIVNKLQSILTKGTL
>Mature_693_residues
MVRTSTHWLLDALLSSKPELVLPDKYNLSMDTREAWHQLQETLRLPASAISEALSLAYSTDGINIQEFKPPRDNPFNERV
CREIGILPFDLSVNEPVIATYDPRLTPEQHEQIRFILRRDFKLAILSPDEIDIGITRLFSTIDHDYRTIDLTSASVEENE
TSQLAKAILRSAIDKRASDIHMHPMVGGAAIRFRVDGILERIASLSKQQHDGLSRFFTMNAGLDVNPLIAQDGRMRLIYG
QREIDVRLSLLPVFDGMRIVCRLLEQGKQFSLVNSGFSPSDYRALKRLVSYGSGIVLLTGPTGSGKTSTLYALLSELNSV
DVNIMTLENPVEYVLPGISQVQINDAQGLSFGDTLRSILRQDPDIVLVGEIRDAETAKIASQAALTGHIVLSTLHTNDAM
ATVPRLLDLGINPSVLADALVGVVSQRLVRKLCSFCKTKVTKPLKPEEQEYERITGELPSCRALGCDECHYTGYTGRLPV
TEVMEVSPEMRAVMLSGCNNVKDLTEANKGFQSQMAVSAGQWIVSGETTAQEISRELGLRFWRELAMVNDKAPNMLLDNF
SGSNPLSEDRLKVLLISDNKGLQSALAESFQYGLVFVNNEHKASEILKKDTSVIAILIDSALMQSTPAEWLGKLREQLAW
SGTPVLFLIPPNQNETKILLNKFHAHAMEYDENMTPPTEIVNKLQSILTKGTL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=378, Percent_Identity=43.6507936507937, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1786296, Length=401, Percent_Identity=37.4064837905237, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=34.3971631205674, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 76955; Mature: 76955

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRTSTHWLLDALLSSKPELVLPDKYNLSMDTREAWHQLQETLRLPASAISEALSLAYST
CCCCCHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DGINIQEFKPPRDNPFNERVCREIGILPFDLSVNEPVIATYDPRLTPEQHEQIRFILRRD
CCCEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCC
FKLAILSPDEIDIGITRLFSTIDHDYRTIDLTSASVEENETSQLAKAILRSAIDKRASDI
CEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCE
HMHPMVGGAAIRFRVDGILERIASLSKQQHDGLSRFFTMNAGLDVNPLIAQDGRMRLIYG
EECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCEECCCCCEEEEEC
QREIDVRLSLLPVFDGMRIVCRLLEQGKQFSLVNSGFSPSDYRALKRLVSYGSGIVLLTG
CCEEEEEEEEEEHHCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEC
PTGSGKTSTLYALLSELNSVDVNIMTLENPVEYVLPGISQVQINDAQGLSFGDTLRSILR
CCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
QDPDIVLVGEIRDAETAKIASQAALTGHIVLSTLHTNDAMATVPRLLDLGINPSVLADAL
CCCCEEEEECCCCCHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
VGVVSQRLVRKLCSFCKTKVTKPLKPEEQEYERITGELPSCRALGCDECHYTGYTGRLPV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
TEVMEVSPEMRAVMLSGCNNVKDLTEANKGFQSQMAVSAGQWIVSGETTAQEISRELGLR
HHHHHCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
FWRELAMVNDKAPNMLLDNFSGSNPLSEDRLKVLLISDNKGLQSALAESFQYGLVFVNNE
HHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
HKASEILKKDTSVIAILIDSALMQSTPAEWLGKLREQLAWSGTPVLFLIPPNQNETKILL
HHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
NKFHAHAMEYDENMTPPTEIVNKLQSILTKGTL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRTSTHWLLDALLSSKPELVLPDKYNLSMDTREAWHQLQETLRLPASAISEALSLAYST
CCCCCHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DGINIQEFKPPRDNPFNERVCREIGILPFDLSVNEPVIATYDPRLTPEQHEQIRFILRRD
CCCEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCC
FKLAILSPDEIDIGITRLFSTIDHDYRTIDLTSASVEENETSQLAKAILRSAIDKRASDI
CEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCE
HMHPMVGGAAIRFRVDGILERIASLSKQQHDGLSRFFTMNAGLDVNPLIAQDGRMRLIYG
EECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCEECCCCCEEEEEC
QREIDVRLSLLPVFDGMRIVCRLLEQGKQFSLVNSGFSPSDYRALKRLVSYGSGIVLLTG
CCEEEEEEEEEEHHCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEC
PTGSGKTSTLYALLSELNSVDVNIMTLENPVEYVLPGISQVQINDAQGLSFGDTLRSILR
CCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
QDPDIVLVGEIRDAETAKIASQAALTGHIVLSTLHTNDAMATVPRLLDLGINPSVLADAL
CCCCEEEEECCCCCHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
VGVVSQRLVRKLCSFCKTKVTKPLKPEEQEYERITGELPSCRALGCDECHYTGYTGRLPV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
TEVMEVSPEMRAVMLSGCNNVKDLTEANKGFQSQMAVSAGQWIVSGETTAQEISRELGLR
HHHHHCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
FWRELAMVNDKAPNMLLDNFSGSNPLSEDRLKVLLISDNKGLQSALAESFQYGLVFVNNE
HHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
HKASEILKKDTSVIAILIDSALMQSTPAEWLGKLREQLAWSGTPVLFLIPPNQNETKILL
HHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
NKFHAHAMEYDENMTPPTEIVNKLQSILTKGTL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]