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The map label for this gene is purA2 [H]

Identifier: 160876437

GI number: 160876437

Start: 3928539

End: 3929795

Strand: Direct

Name: purA2 [H]

Synonym: Sbal195_3331

Alternate gene names: 160876437

Gene position: 3928539-3929795 (Clockwise)

Preceding gene: 160876435

Following gene: 160876439

Centisome position: 73.47

GC content: 48.69

Gene sequence:

>1257_bases
ATGCCGTCGATCGTGGTTGTGGGTGCAAATTGGGGTGATGAAGGCAAAGGCCGCATCGTAGATTATTTAGCCGCGGATGC
CGCTGCCAGTATTCGCTTTCAAGGCGGTAACAACGCCGGACATACCGTAGTCAACGACTATGGCACCTTTAAATTACACC
AATTACCAAGTGGCATTTTCAACCCTAACTGCATCGCAGTATTAGGCCCTGGCATGGTCATCAGCCCTGAAAAGCTCAGC
ATAGAAATTGCTGAAGTACAAGCGACCGGTGTTGACGTCAAGTTATGCATTTCTGACCGTGCGACCCTGTGCTTACCTTT
ACATGCATTAGAAGATACCTTAGAAGAACAACGTCTTGGCGATTTAGCCTATGGTTCAACCCGTCAAGGGATTGCACCTG
CCTATGGTGATCGCGTGATGAAAAAAGGCATTTTAGTCGGTTGGTTAAACCAACCTGAAGTGCTGCAAGAACGTATTCAA
TTTATGCTCGACTGGAAAATGCCACAATTAAAGGCACTTTATCCAAGCTGCGATTTTAATCAAACCGCCGCTGAAATGAC
CGCATGGTTGTTAGAAGTGACTGCGCCATGGCGTCCATTTATTTGTAATGTGACTGAGCCATTAAAAGCATTACAAAAAC
AAGATGCAAGCTTACTGTTTGAAGCGCAATTAGGTGCTGGCCGCGACTTAGTCTACGGCGAATATCCTTATACCACCTCA
TCAAACGTGACTGCGGCTTACGCAGGTATCGGTAGTGGTTTACCAGCCCTGCGCCCAGAGCGCGTGATTGCTGTGGCGAA
AGCCTTTAGCTCATCTGTGGGTACTGGCACTTTAGTGACGGCTATGGAAGAGCAAGACGCCTTCCGTGAAAACGCCAATG
AATTTGGTGCAACCACAGGCCGTCCACGTGACATGGGTTACTTCGATGCTGTTGCCACCCGTAATGGTGTGGAGCTGCAA
GCGGCAACTGAAATTGCCCTAACTAAGATTGATTGCCTGTCAGGCATGAAAGATCTGAAAATATGTGTTGCCTATGAAGG
TGAGCACACTGAGAACCCAATTTGGCCACAAACTGCCGCGTTGAGCCCAGTGTATGAGCAAATGCCAAGCTGGAGCGAAG
ATATTACCGGTTGTCGCACCTTCGAGAGCCTGCCAGTGGCCGCACAACACTATGTTGAACGTATTGAAGCCCTAATGGGT
GTGCGCGTTAGCATGGTTTCTGTTGGACCTGAGCGCGATCAAATGATCATCCGCTAG

Upstream 100 bases:

>100_bases
ATGATATATGAAATCCTTATTGAGATGATAACCATAATTAATTTCTCTTATTCAAATGACGGGTCTAGCATAGCGTCTTA
ATCAATGAGGAGATGTTGTT

Downstream 100 bases:

>100_bases
TCGCGAAGTTTACCCTGCAAAAAAGCCATATTCGCTCCGGTGAATATGGCTTTTTACTTTTATATCGTTTGTCATTGAAG
AATCTCTCAAGACCTTGGTA

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPSIVVVGANWGDEGKGRIVDYLAADAAASIRFQGGNNAGHTVVNDYGTFKLHQLPSGIFNPNCIAVLGPGMVISPEKLS
IEIAEVQATGVDVKLCISDRATLCLPLHALEDTLEEQRLGDLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQ
FMLDWKMPQLKALYPSCDFNQTAAEMTAWLLEVTAPWRPFICNVTEPLKALQKQDASLLFEAQLGAGRDLVYGEYPYTTS
SNVTAAYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDAFRENANEFGATTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLSGMKDLKICVAYEGEHTENPIWPQTAALSPVYEQMPSWSEDITGCRTFESLPVAAQHYVERIEALMG
VRVSMVSVGPERDQMIIR

Sequences:

>Translated_418_residues
MPSIVVVGANWGDEGKGRIVDYLAADAAASIRFQGGNNAGHTVVNDYGTFKLHQLPSGIFNPNCIAVLGPGMVISPEKLS
IEIAEVQATGVDVKLCISDRATLCLPLHALEDTLEEQRLGDLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQ
FMLDWKMPQLKALYPSCDFNQTAAEMTAWLLEVTAPWRPFICNVTEPLKALQKQDASLLFEAQLGAGRDLVYGEYPYTTS
SNVTAAYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDAFRENANEFGATTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLSGMKDLKICVAYEGEHTENPIWPQTAALSPVYEQMPSWSEDITGCRTFESLPVAAQHYVERIEALMG
VRVSMVSVGPERDQMIIR
>Mature_417_residues
PSIVVVGANWGDEGKGRIVDYLAADAAASIRFQGGNNAGHTVVNDYGTFKLHQLPSGIFNPNCIAVLGPGMVISPEKLSI
EIAEVQATGVDVKLCISDRATLCLPLHALEDTLEEQRLGDLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQF
MLDWKMPQLKALYPSCDFNQTAAEMTAWLLEVTAPWRPFICNVTEPLKALQKQDASLLFEAQLGAGRDLVYGEYPYTTSS
NVTAAYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDAFRENANEFGATTGRPRDMGYFDAVATRNGVELQA
ATEIALTKIDCLSGMKDLKICVAYEGEHTENPIWPQTAALSPVYEQMPSWSEDITGCRTFESLPVAAQHYVERIEALMGV
RVSMVSVGPERDQMIIR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=34.4907407407407, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=33.9578454332553, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI40316944, Length=400, Percent_Identity=31.5, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=37.3831775700935, Blast_Score=277, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=32.1759259259259, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=32.1759259259259, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=30.8584686774942, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=34.3529411764706, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45456; Mature: 45325

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVVVGANWGDEGKGRIVDYLAADAAASIRFQGGNNAGHTVVNDYGTFKLHQLPSGIF
CCCEEEEECCCCCCCCCCEEEHHHHCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCCC
NPNCIAVLGPGMVISPEKLSIEIAEVQATGVDVKLCISDRATLCLPLHALEDTLEEQRLG
CCCEEEEECCCEEECCCCCEEEEEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQFMLDWKMPQLKALYPSCDFN
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCCCC
QTAAEMTAWLLEVTAPWRPFICNVTEPLKALQKQDASLLFEAQLGAGRDLVYGEYPYTTS
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDAFRENANEFGATTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCHHHHCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLSGMKDLKICVAYEGEHTENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHH
LSPVYEQMPSWSEDITGCRTFESLPVAAQHYVERIEALMGVRVSMVSVGPERDQMIIR
CCHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCEECC
>Mature Secondary Structure 
PSIVVVGANWGDEGKGRIVDYLAADAAASIRFQGGNNAGHTVVNDYGTFKLHQLPSGIF
CCEEEEECCCCCCCCCCEEEHHHHCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCCC
NPNCIAVLGPGMVISPEKLSIEIAEVQATGVDVKLCISDRATLCLPLHALEDTLEEQRLG
CCCEEEEECCCEEECCCCCEEEEEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQFMLDWKMPQLKALYPSCDFN
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCCCC
QTAAEMTAWLLEVTAPWRPFICNVTEPLKALQKQDASLLFEAQLGAGRDLVYGEYPYTTS
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDAFRENANEFGATTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCHHHHCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLSGMKDLKICVAYEGEHTENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHH
LSPVYEQMPSWSEDITGCRTFESLPVAAQHYVERIEALMGVRVSMVSVGPERDQMIIR
CCHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA