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The map label for this gene is gatA [H]

Identifier: 160876389

GI number: 160876389

Start: 3865135

End: 3866889

Strand: Direct

Name: gatA [H]

Synonym: Sbal195_3283

Alternate gene names: 160876389

Gene position: 3865135-3866889 (Clockwise)

Preceding gene: 160876388

Following gene: 160876392

Centisome position: 72.28

GC content: 54.25

Gene sequence:

>1755_bases
ATGTCACTGTTGTTGCAACACAATTGGATTTCAGCGGGAGGCAGTCAAATGTTTGAAGTCACTGAAGTTTCTATTGCCGA
GCTGCGTGCAGCACTCGAATCCGGCCGCACCACAGCAGTGGAGCTGGTGAAGTCTTATCTCGCACGGGTTGAGGCGTATG
ACGGCCCAGCTACGCAGACTAAGCTCAATGCGTTAGTGGTGCCCAATCCCGATGCGCTCAAAGAAGCTGAGGCGTCAGAT
GCGCGCCGCGCCCGTGGTGAAACCTTAAGCCCACTTGAGGGGATTACGTATACCGCTAAGGACAGCTATCTAGTGAAGGG
GCTGACGGCGGCGTCTGGCAGCCCAGCCTTTAAGGATTTAGTGGCGCAATACGATGCCTTCGCCGTTGAGCGCCTACGCG
CCGCGGGTGCTATTTGCTTAGGCAAAACCAATATGCCACCCATGGCCAATGGCGGTATGCAGCGTGGTCACTATGGCCGC
GCTGAAAGCCCATACAATGCTGAGTATCTTACTGCACCATTCGCTTCTGGCTCTTCCAATGGGGCGGGCACGGGCACGGC
AGCAAGTTACTCTGCTTTTGGTCTTGCTGAAGAAACTTGGTCGAGCGGTCGTGGTCCGGCATCTAATAACGGTTTGTGTG
CCTACACTCCGTCACGGGGCGTGATTTCCGTGCGCGGTAACTGGCCGTTAACGCCGACCATGGATGTAGTGGTGCCTTAC
ACTCGCACTATGGCAGATATGCTCGAAGTGTTGGATGTGATTGTGGCCGATGACCCAATTACCCGTGGCGACCTCTGGCG
TCTACAAACTTGGGTCGAAATTCCCAAAGCATCAAGTGTCCGTCCAGCGTCTTATCTGGCGCTTGCCGCTCAAGCCGACA
CGCTTAAAGGTAAGCGTTTTGGTGTGCCGCGCATGTACATCAATAAGGATGAATTGGCGGGAACCAGCGAAAATCCGGGC
ATTGGTGGCCCAACGGGCCAACGTATCCACACTCGCCAATCGGTTATCGATTTGTGGGAAGCGGCTCGTAAAGCCTTAGA
AGCTGCCGGCGCCGAAGTGATTGAAGTTGATTTTCCGCTGGTATCAAATTGTGAAGGCGACCGCCCAGGCGCGCCGACCG
TGTTTAACCGTGGCATAGTCACGCCTCAGTTCCTCCATGATGAATTATGGGAACTCTCAGGTTGGGCGTTCGATGAATTC
CTGCGTGCTAACAATGACCCTAAACTCAACAAACTTGCTGATGTTGTTGGGCATTTGATTTTCCCCCACGACTTAGGCAC
CTTGCCAAACCGTGAAGGTGACTTAGCGGCAGGCATGGAAGAGTACGTCAACATGGCTAAGCGTGGTCTTAAATCCTATG
ACCAGATCGACAGTATTCCCGATGGTCTGCGTGGCTTAGAGAAGACGCGTAAGCTAGATCTCGAAGACTGGATGGACGGG
CTAAAGCTTGATGCCGTGCTGTTTCCAACCGTGGCAGATGTCGGCCCTGCCGATGCCCATGTGAATCCGGCTTCAGCCGA
TATCGCTTGGAGCAACGGAGTTTGGGTGGCAAACGGAAACCTCGCGATTCGTCACTTAGGGGTACCGACTGTGACTGTAC
CTATGGGCGTGATGGCCGATATTGGCATGCCAGTGGGGTTAACCTTCGCGGGCCGCGCCTATGACGATAATAACCTGCTT
AAATTTGCAGGTGCATTTGAGTCGACAGGTGAAAGGCGCATGATCCCGCCACGCACGCCAAAGCTTGGTGAATAA

Upstream 100 bases:

>100_bases
TGCCCGTGAGATTTTATTAGGTGGTGGCAATATCCATTGTATTACCCAACAGATCCCCTCTGGAAAGTAAGTCAACCTAA
GGAGAACAGCATGACGATTG

Downstream 100 bases:

>100_bases
GCGCCAAGACAATACATAAACCTAAGCACTAACTGTAAGCATTAAATGTAAGCACTAAATGTAAGCACTAAATGCATGAG
CTTAGAAATAAAAACGGGAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSLLLQHNWISAGGSQMFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVPNPDALKEAEASD
ARRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGR
AESPYNAEYLTAPFASGSSNGAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPY
TRTMADMLEVLDVIVADDPITRGDLWRLQTWVEIPKASSVRPASYLALAAQADTLKGKRFGVPRMYINKDELAGTSENPG
IGGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEF
LRANNDPKLNKLADVVGHLIFPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDG
LKLDAVLFPTVADVGPADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDNNLL
KFAGAFESTGERRMIPPRTPKLGE

Sequences:

>Translated_584_residues
MSLLLQHNWISAGGSQMFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVPNPDALKEAEASD
ARRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGR
AESPYNAEYLTAPFASGSSNGAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPY
TRTMADMLEVLDVIVADDPITRGDLWRLQTWVEIPKASSVRPASYLALAAQADTLKGKRFGVPRMYINKDELAGTSENPG
IGGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEF
LRANNDPKLNKLADVVGHLIFPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDG
LKLDAVLFPTVADVGPADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDNNLL
KFAGAFESTGERRMIPPRTPKLGE
>Mature_583_residues
SLLLQHNWISAGGSQMFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVPNPDALKEAEASDA
RRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGRA
ESPYNAEYLTAPFASGSSNGAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYT
RTMADMLEVLDVIVADDPITRGDLWRLQTWVEIPKASSVRPASYLALAAQADTLKGKRFGVPRMYINKDELAGTSENPGI
GGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEFL
RANNDPKLNKLADVVGHLIFPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDGL
KLDAVLFPTVADVGPADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDNNLLK
FAGAFESTGERRMIPPRTPKLGE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=568, Percent_Identity=25.3521126760563, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI195972892, Length=241, Percent_Identity=28.2157676348548, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=268, Percent_Identity=25.3731343283582, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=253, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=304, Percent_Identity=26.6447368421053, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161078093, Length=254, Percent_Identity=33.4645669291339, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24644968, Length=251, Percent_Identity=30.6772908366534, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21356731, Length=244, Percent_Identity=28.2786885245902, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45550774, Length=259, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648435, Length=254, Percent_Identity=26.7716535433071, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648437, Length=254, Percent_Identity=26.7716535433071, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652985, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922090, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652981, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652983, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 62647; Mature: 62516

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLLQHNWISAGGSQMFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQT
CCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCE
KLNALVVPNPDALKEAEASDARRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDL
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHHH
VAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFASGSSN
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPY
CCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHEEECH
TRTMADMLEVLDVIVADDPITRGDLWRLQTWVEIPKASSVRPASYLALAAQADTLKGKRF
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHEEEEHHHHHCCCCCC
GVPRMYINKDELAGTSENPGIGGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPL
CCCCEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCH
VSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVVGHLI
HCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHH
FPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCC
LKLDAVLFPTVADVGPADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMAD
CEECEEEECHHHCCCCCCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHH
IGMPVGLTFAGRAYDDNNLLKFAGAFESTGERRMIPPRTPKLGE
CCCCCCEEEECCEECCCCEEEEECCHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLLLQHNWISAGGSQMFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQT
CEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCE
KLNALVVPNPDALKEAEASDARRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDL
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHHH
VAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFASGSSN
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPY
CCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHEEECH
TRTMADMLEVLDVIVADDPITRGDLWRLQTWVEIPKASSVRPASYLALAAQADTLKGKRF
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHEEEEHHHHHCCCCCC
GVPRMYINKDELAGTSENPGIGGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPL
CCCCEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCH
VSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVVGHLI
HCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHH
FPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCC
LKLDAVLFPTVADVGPADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMAD
CEECEEEECHHHCCCCCCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHH
IGMPVGLTFAGRAYDDNNLLKFAGAFESTGERRMIPPRTPKLGE
CCCCCCEEEECCEECCCCEEEEECCHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA