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The map label for this gene is ispD [H]

Identifier: 160876383

GI number: 160876383

Start: 3858058

End: 3858849

Strand: Reverse

Name: ispD [H]

Synonym: Sbal195_3277

Alternate gene names: 160876383

Gene position: 3858849-3858058 (Counterclockwise)

Preceding gene: 160876384

Following gene: 160876382

Centisome position: 72.16

GC content: 51.39

Gene sequence:

>792_bases
ATGTCAGAGCTAGGACATTTAGATCTTGGATTAAGCGAACAATCCAATCCAGGCAGTCAATCTGAGCCGGTAATGCAAAC
TGAATTGCAGCGCCAAGCTGAGCATATCACTCAAGCTGTGGTGGCTATTGTTCCCGCTGCGGGCATTGGTAGCCGCATGG
GCGCCGACAGACCTAAACAATATCTTGAGGTTTGCGGCCAGAGTATTCTCGGCCATACTTTAGATAAGCTGTTGTCTCAC
CCGCACATTTCCAACGTCATCGTCGCCTTACATCCGAATGATACTATCTTCAATACCTTGCCACAGGCCGCGCATCCTAA
ATTGACCACAGTATTAGGTGGCGGTGAGCGCGCTGATTCTGTGTTGGCGGCGCTGCAAATTGCGCCCGAGCACAGTTGGG
CATTAGTGCATGATGCGGCAAGACCTTGTTTAACCGCGACGGATATCGACAAGTTACTCGCATCCAGAGTGCAATTTCCC
CAAGGCGCGATTTTGGCCATGCCAGTGCGCGATACCATGAAGCGCGCCAATAAACAGGGCGAAATCTGCGCTACTGTGTG
CCGCGATAATTTATGGCATGCATTAACGCCGCAGTTATTTCAGGCAGCGCAACTAAAAGATTACCTCAATGCCGCTCTGG
CGGCAGGTGTGGTGGTGACAGATGAGGCTTCGGCGATGGAATGGGCGGGCATTTCACCTGGACTCGTTTCGGGCCGTGCT
GACAATATAAAAGTGACGCATCCCGATGATTTGGGATTGGCCGAATTGTTTTTAATGCGTGCCGCAAAATAG

Upstream 100 bases:

>100_bases
GCGTAACGAACTTGGCATGGTCAAAGAAGGTGAAACCTTCTATCGCGTCGTGGGTGGTGATCGCTCAGTATCGAGTCCAT
CCCAGTAATATGGAGTTAGG

Downstream 100 bases:

>100_bases
TCATAATCCAATTAAAATAATACGTTAGGAATATTAAATGAAAATCCGTATCGGCCATGGTTTCGATGTGCATAAATTTG
GTGCTGCACGGCCCCTCATT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSELGHLDLGLSEQSNPGSQSEPVMQTELQRQAEHITQAVVAIVPAAGIGSRMGADRPKQYLEVCGQSILGHTLDKLLSH
PHISNVIVALHPNDTIFNTLPQAAHPKLTTVLGGGERADSVLAALQIAPEHSWALVHDAARPCLTATDIDKLLASRVQFP
QGAILAMPVRDTMKRANKQGEICATVCRDNLWHALTPQLFQAAQLKDYLNAALAAGVVVTDEASAMEWAGISPGLVSGRA
DNIKVTHPDDLGLAELFLMRAAK

Sequences:

>Translated_263_residues
MSELGHLDLGLSEQSNPGSQSEPVMQTELQRQAEHITQAVVAIVPAAGIGSRMGADRPKQYLEVCGQSILGHTLDKLLSH
PHISNVIVALHPNDTIFNTLPQAAHPKLTTVLGGGERADSVLAALQIAPEHSWALVHDAARPCLTATDIDKLLASRVQFP
QGAILAMPVRDTMKRANKQGEICATVCRDNLWHALTPQLFQAAQLKDYLNAALAAGVVVTDEASAMEWAGISPGLVSGRA
DNIKVTHPDDLGLAELFLMRAAK
>Mature_262_residues
SELGHLDLGLSEQSNPGSQSEPVMQTELQRQAEHITQAVVAIVPAAGIGSRMGADRPKQYLEVCGQSILGHTLDKLLSHP
HISNVIVALHPNDTIFNTLPQAAHPKLTTVLGGGERADSVLAALQIAPEHSWALVHDAARPCLTATDIDKLLASRVQFPQ
GAILAMPVRDTMKRANKQGEICATVCRDNLWHALTPQLFQAAQLKDYLNAALAAGVVVTDEASAMEWAGISPGLVSGRAD
NIKVTHPDDLGLAELFLMRAAK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=54.954954954955, Blast_Score=236, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 28082; Mature: 27951

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELGHLDLGLSEQSNPGSQSEPVMQTELQRQAEHITQAVVAIVPAAGIGSRMGADRPKQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
YLEVCGQSILGHTLDKLLSHPHISNVIVALHPNDTIFNTLPQAAHPKLTTVLGGGERADS
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCCEEEEECCCHHHHH
VLAALQIAPEHSWALVHDAARPCLTATDIDKLLASRVQFPQGAILAMPVRDTMKRANKQG
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCC
EICATVCRDNLWHALTPQLFQAAQLKDYLNAALAAGVVVTDEASAMEWAGISPGLVSGRA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHCCCCCCCCCCCC
DNIKVTHPDDLGLAELFLMRAAK
CCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SELGHLDLGLSEQSNPGSQSEPVMQTELQRQAEHITQAVVAIVPAAGIGSRMGADRPKQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
YLEVCGQSILGHTLDKLLSHPHISNVIVALHPNDTIFNTLPQAAHPKLTTVLGGGERADS
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCCEEEEECCCHHHHH
VLAALQIAPEHSWALVHDAARPCLTATDIDKLLASRVQFPQGAILAMPVRDTMKRANKQG
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCC
EICATVCRDNLWHALTPQLFQAAQLKDYLNAALAAGVVVTDEASAMEWAGISPGLVSGRA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHCCCCCCCCCCCC
DNIKVTHPDDLGLAELFLMRAAK
CCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]