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The map label for this gene is alaS [H]

Identifier: 160876373

GI number: 160876373

Start: 3845236

End: 3847860

Strand: Reverse

Name: alaS [H]

Synonym: Sbal195_3267

Alternate gene names: 160876373

Gene position: 3847860-3845236 (Counterclockwise)

Preceding gene: 160876374

Following gene: 160876372

Centisome position: 71.96

GC content: 48.38

Gene sequence:

>2625_bases
ATGTATCAAACTACAGCAGAGCTTAGAAGCGCTTTCCTCGAATTTTTCCGCAGCAATGGTCATCAAGTTGTGGACAGTAG
TTCATTAGTGCCAGGCAACGATCCCACACTTTTATTTACCAATGCAGGCATGAACCAGTTCAAGGACGTGTTTCTTGGCA
TGGACAAACGCAATTATACGCGTGCGACCACTGCTCAACGTTGTGTACGCGCCGGTGGTAAGCATAACGATTTAGATAAC
GTCGGTTATACAGCACGTCATCACACCTTTTTCGAAATGCTGGGTAACTTCAGTTTTGGTGATTATTTCAAAGAAGAAGC
GATTCGCTTTGGCTGGACTTTCTTAACTGAAACTTTAAAGCTGCCGAAAGAACGTCTGTGTGTCACTATTTATCAAACAG
ATGATGAAGCCTTTGAAATCTGGAATAAAAAGATTGGTGTTGCTGCCGAGAACATTATCCGTATCGGCGACAACAAAGGT
GCTGCTTATGCGTCAGATAACTTCTGGCAAATGGGTGATACCGGTCCTTGCGGCCCATGTTCTGAGATTTTCTATGACCA
TGGCGACCATATTTGGGGCGGCCGTCCTGGCAGTCCCGAAGAAGATGGCGACCGTTTCATCGAAATCTGGAACATAGTGT
TCATGCAGTACAATCGTCAAGCGTCGGGTGAAATGCTACCTTTGCCTAAGCCATCGGTTGATACTGGCATGGGGATTGAG
CGTATTGCCGCGATTATGCAAGGCGTACATTCTAACTACGAAATCGATATCTTCCGTACGCTGATCGCCAAAGCGGCTGA
AATCATCGGCGTAAGCGATTTAACCGAGAAGTCACTGCGCGTCATTGCCGACCATATCCGTTCATGTGCATTCCTTATTG
CCGATGGCGTTATGCCGTCGAATGAAGGCCGCGGTTATGTGCTGCGCCGCATTATCCGCCGCGCAGTTCGCCATGGTAAC
AAGTTAGGCGCGACCGAAGCTTTCTTCTATAAGCTGGTTCCGTCTTTGATTGCGGTGATGGGCGATGCGGCCAAAGGGTT
AGCTGAGACTCAAGCGATTGTTGAAAAAGCACTGAAAGCGGAAGAAGAGCAATTTGCCCGTACGCTTGAACGTGGTTTAG
GCATTTTAGATGCGGCTTTAAACGAGTTAAAAGGTGACACCTTAGACGGTGAAACGGTTTTTAAGCTGTATGACACCTAT
GGCTTCCCAATGGATTTAACCGCTGACGTGTGTCGCGAGCGCAACATCATTGTCGATGAAGCAGGTTTCGAAGTCGCTAT
GGCTGAACAACGTAGCCGCGCCCAAGCTGCCGGTAACTTTGGTGCAGATTACAACGCTGCACTGAAAATCGATGCTGAAA
CCGCATTCAGTGGTTATACCGAATTAGCGGGCCAAGCTAAGGTCACCGCGATTTATCAAAACGGTGAATCAGTGACGGCG
ATCAAAGCGGGTGACGAAGCGGTTGTTGTACTCGACGTGACACCTTTCTACGCTGAATCTGGCGGTCAAGTGGGCGATAA
AGGCCAACTGGTTGCGAACGGTGTTGAATTTACCGTGAACGACACGCAAAAGTACGGTCAAGCAACGGGCCATCAAGGTG
TTTTAGCTACAGGTAACTTGAGTGTTGGCCAAGTTGTTGAAGCGAAAGTCGACAAAAAACTGCGTCACCGTACTCAGTTA
AACCATTCTGTTACGCATTTACTGCATGCCGCACTGCGTCAAGTGCTCGGTACTCACGTTTCGCAAAAAGGTTCTTTGGT
TGATCCTGAGCGTTTGCGTTTTGACTTCTCCCATTTCGAAGGCGTAAAAGCGACGGAATTAAAAGAAGTTGAAGAATTAG
TTAACACTCAAATTCGTCGTAACCATGAGCTGAAAACCGCTGAAATGGGTATAGATGAAGCCAAAGAGAAAGGCGCAATG
GCGCTCTTTGGTGAGAAATATGACTCACAAGTGCGTGTTGTGACTATGGGGGATTTCTCTATCGAATTGTGTGGTGGTAC
CCATGTGGGCCGCACGGGCGATATTGGCTTATTCAAAATCACCTCTGAAGCGGGTATTGCTGCGGGTGTACGTCGTATCG
AAGCCGTTACTGGCGCTGCTGCTATGGCCTATGTGGCGCAGCAACAAGCTGAACTGGAAGAAGCTGCAGCCTTATTGAAA
GGCGATGCCAACTCAGTCGTTGCTAAGTTAAAAGCCCAGCTCGATAAGATGAAGCAACTCGAAAAAGAAATGGCGCAGTT
GAAAGACAAGCTCGCGGCTGCAGCGAGTGCTGATTTAGTGGGCGATGCCGTTGTGGTTAATGGCGTTAACGTGCTGATCA
AAAAGTTAGACGGCGTTGAGGCAAGTTCATTACGCGGTTTGCAAGATGAATTAAAACAAAAATTGAAATCGGCCATTATC
GTGCTTGGCACTGCGCAGGAAGGGAAAGTTAACCTGATCGCCGGTGTGAGTAACGATCTGATCGGCAAAGTCAAAGCGGG
TGAGCTAGTCGCTATGGTCGCTGCACAAGTGGGCGGTAAAGGCGGCGGTCGTCCTGATATGGCTCAAGCGGGCGGTAGCC
AGCCAGAAAATCTGGATGCGGCATTGGCTCAAGTATTACCTTGGATTACTGAGCGTCTAGCTTAA

Upstream 100 bases:

>100_bases
GCTGGTCGTCCTTACTCAGTCTGCTTATAATGCTGGGCAAATAGAAATACGGCTAGCCTAAGGCGTAGCTGGTAATGCTT
ACCCAATTCAGGATGATTTC

Downstream 100 bases:

>100_bases
GAGGTTGCTGTGCCCCAAAAACGAAAGCTTAGTGGTAGCAAGCTTTTTGTGAAAAAGTTCGGCGGCACTTCGGTGGGTTC
TATTGAACGTATCGAAGCGG

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MYQTTAELRSAFLEFFRSNGHQVVDSSSLVPGNDPTLLFTNAGMNQFKDVFLGMDKRNYTRATTAQRCVRAGGKHNDLDN
VGYTARHHTFFEMLGNFSFGDYFKEEAIRFGWTFLTETLKLPKERLCVTIYQTDDEAFEIWNKKIGVAAENIIRIGDNKG
AAYASDNFWQMGDTGPCGPCSEIFYDHGDHIWGGRPGSPEEDGDRFIEIWNIVFMQYNRQASGEMLPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFRTLIAKAAEIIGVSDLTEKSLRVIADHIRSCAFLIADGVMPSNEGRGYVLRRIIRRAVRHGN
KLGATEAFFYKLVPSLIAVMGDAAKGLAETQAIVEKALKAEEEQFARTLERGLGILDAALNELKGDTLDGETVFKLYDTY
GFPMDLTADVCRERNIIVDEAGFEVAMAEQRSRAQAAGNFGADYNAALKIDAETAFSGYTELAGQAKVTAIYQNGESVTA
IKAGDEAVVVLDVTPFYAESGGQVGDKGQLVANGVEFTVNDTQKYGQATGHQGVLATGNLSVGQVVEAKVDKKLRHRTQL
NHSVTHLLHAALRQVLGTHVSQKGSLVDPERLRFDFSHFEGVKATELKEVEELVNTQIRRNHELKTAEMGIDEAKEKGAM
ALFGEKYDSQVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEAGIAAGVRRIEAVTGAAAMAYVAQQQAELEEAAALLK
GDANSVVAKLKAQLDKMKQLEKEMAQLKDKLAAAASADLVGDAVVVNGVNVLIKKLDGVEASSLRGLQDELKQKLKSAII
VLGTAQEGKVNLIAGVSNDLIGKVKAGELVAMVAAQVGGKGGGRPDMAQAGGSQPENLDAALAQVLPWITERLA

Sequences:

>Translated_874_residues
MYQTTAELRSAFLEFFRSNGHQVVDSSSLVPGNDPTLLFTNAGMNQFKDVFLGMDKRNYTRATTAQRCVRAGGKHNDLDN
VGYTARHHTFFEMLGNFSFGDYFKEEAIRFGWTFLTETLKLPKERLCVTIYQTDDEAFEIWNKKIGVAAENIIRIGDNKG
AAYASDNFWQMGDTGPCGPCSEIFYDHGDHIWGGRPGSPEEDGDRFIEIWNIVFMQYNRQASGEMLPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFRTLIAKAAEIIGVSDLTEKSLRVIADHIRSCAFLIADGVMPSNEGRGYVLRRIIRRAVRHGN
KLGATEAFFYKLVPSLIAVMGDAAKGLAETQAIVEKALKAEEEQFARTLERGLGILDAALNELKGDTLDGETVFKLYDTY
GFPMDLTADVCRERNIIVDEAGFEVAMAEQRSRAQAAGNFGADYNAALKIDAETAFSGYTELAGQAKVTAIYQNGESVTA
IKAGDEAVVVLDVTPFYAESGGQVGDKGQLVANGVEFTVNDTQKYGQATGHQGVLATGNLSVGQVVEAKVDKKLRHRTQL
NHSVTHLLHAALRQVLGTHVSQKGSLVDPERLRFDFSHFEGVKATELKEVEELVNTQIRRNHELKTAEMGIDEAKEKGAM
ALFGEKYDSQVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEAGIAAGVRRIEAVTGAAAMAYVAQQQAELEEAAALLK
GDANSVVAKLKAQLDKMKQLEKEMAQLKDKLAAAASADLVGDAVVVNGVNVLIKKLDGVEASSLRGLQDELKQKLKSAII
VLGTAQEGKVNLIAGVSNDLIGKVKAGELVAMVAAQVGGKGGGRPDMAQAGGSQPENLDAALAQVLPWITERLA
>Mature_874_residues
MYQTTAELRSAFLEFFRSNGHQVVDSSSLVPGNDPTLLFTNAGMNQFKDVFLGMDKRNYTRATTAQRCVRAGGKHNDLDN
VGYTARHHTFFEMLGNFSFGDYFKEEAIRFGWTFLTETLKLPKERLCVTIYQTDDEAFEIWNKKIGVAAENIIRIGDNKG
AAYASDNFWQMGDTGPCGPCSEIFYDHGDHIWGGRPGSPEEDGDRFIEIWNIVFMQYNRQASGEMLPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFRTLIAKAAEIIGVSDLTEKSLRVIADHIRSCAFLIADGVMPSNEGRGYVLRRIIRRAVRHGN
KLGATEAFFYKLVPSLIAVMGDAAKGLAETQAIVEKALKAEEEQFARTLERGLGILDAALNELKGDTLDGETVFKLYDTY
GFPMDLTADVCRERNIIVDEAGFEVAMAEQRSRAQAAGNFGADYNAALKIDAETAFSGYTELAGQAKVTAIYQNGESVTA
IKAGDEAVVVLDVTPFYAESGGQVGDKGQLVANGVEFTVNDTQKYGQATGHQGVLATGNLSVGQVVEAKVDKKLRHRTQL
NHSVTHLLHAALRQVLGTHVSQKGSLVDPERLRFDFSHFEGVKATELKEVEELVNTQIRRNHELKTAEMGIDEAKEKGAM
ALFGEKYDSQVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEAGIAAGVRRIEAVTGAAAMAYVAQQQAELEEAAALLK
GDANSVVAKLKAQLDKMKQLEKEMAQLKDKLAAAASADLVGDAVVVNGVNVLIKKLDGVEASSLRGLQDELKQKLKSAII
VLGTAQEGKVNLIAGVSNDLIGKVKAGELVAMVAAQVGGKGGGRPDMAQAGGSQPENLDAALAQVLPWITERLA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=976, Percent_Identity=37.0901639344262, Blast_Score=541, Evalue=1e-153,
Organism=Homo sapiens, GI38569417, Length=978, Percent_Identity=32.6175869120654, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI1789048, Length=875, Percent_Identity=69.4857142857143, Blast_Score=1252, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=974, Percent_Identity=35.9342915811088, Blast_Score=529, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17536681, Length=754, Percent_Identity=34.6153846153846, Blast_Score=408, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324911, Length=964, Percent_Identity=38.1742738589212, Blast_Score=582, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24582809, Length=967, Percent_Identity=36.7114788004137, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45552267, Length=967, Percent_Identity=36.7114788004137, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24658214, Length=916, Percent_Identity=29.9126637554585, Blast_Score=325, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94731; Mature: 94731

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQTTAELRSAFLEFFRSNGHQVVDSSSLVPGNDPTLLFTNAGMNQFKDVFLGMDKRNYT
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTAQRCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEEAIRFGWTFLTETLK
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LPKERLCVTIYQTDDEAFEIWNKKIGVAAENIIRIGDNKGAAYASDNFWQMGDTGPCGPC
CCCHHEEEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCCEEECCCCEECCCCCCCCCH
SEIFYDHGDHIWGGRPGSPEEDGDRFIEIWNIVFMQYNRQASGEMLPLPKPSVDTGMGIE
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
RIAAIMQGVHSNYEIDIFRTLIAKAAEIIGVSDLTEKSLRVIADHIRSCAFLIADGVMPS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NEGRGYVLRRIIRRAVRHGNKLGATEAFFYKLVPSLIAVMGDAAKGLAETQAIVEKALKA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEEQFARTLERGLGILDAALNELKGDTLDGETVFKLYDTYGFPMDLTADVCRERNIIVDE
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCEEEEC
AGFEVAMAEQRSRAQAAGNFGADYNAALKIDAETAFSGYTELAGQAKVTAIYQNGESVTA
CCCEEHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHCCHHHHCCCEEEEEEEECCCEEEE
IKAGDEAVVVLDVTPFYAESGGQVGDKGQLVANGVEFTVNDTQKYGQATGHQGVLATGNL
EECCCCEEEEEECCCHHHCCCCCCCCCCCEEECCEEEEECCHHHHCCCCCCCCEEEECCC
SVGQVVEAKVDKKLRHRTQLNHSVTHLLHAALRQVLGTHVSQKGSLVDPERLRFDFSHFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHCC
GVKATELKEVEELVNTQIRRNHELKTAEMGIDEAKEKGAMALFGEKYDSQVRVVTMGDFS
CCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHCCCEEEECCCCCCEEEEEEECCEE
IELCGGTHVGRTGDIGLFKITSEAGIAAGVRRIEAVTGAAAMAYVAQQQAELEEAAALLK
EEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDANSVVAKLKAQLDKMKQLEKEMAQLKDKLAAAASADLVGDAVVVNGVNVLIKKLDGVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
ASSLRGLQDELKQKLKSAIIVLGTAQEGKVNLIAGVSNDLIGKVKAGELVAMVAAQVGGK
HHHHCCHHHHHHHHHHCEEEEEECCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHCCC
GGGRPDMAQAGGSQPENLDAALAQVLPWITERLA
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYQTTAELRSAFLEFFRSNGHQVVDSSSLVPGNDPTLLFTNAGMNQFKDVFLGMDKRNYT
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTAQRCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEEAIRFGWTFLTETLK
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LPKERLCVTIYQTDDEAFEIWNKKIGVAAENIIRIGDNKGAAYASDNFWQMGDTGPCGPC
CCCHHEEEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCCEEECCCCEECCCCCCCCCH
SEIFYDHGDHIWGGRPGSPEEDGDRFIEIWNIVFMQYNRQASGEMLPLPKPSVDTGMGIE
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
RIAAIMQGVHSNYEIDIFRTLIAKAAEIIGVSDLTEKSLRVIADHIRSCAFLIADGVMPS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NEGRGYVLRRIIRRAVRHGNKLGATEAFFYKLVPSLIAVMGDAAKGLAETQAIVEKALKA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEEQFARTLERGLGILDAALNELKGDTLDGETVFKLYDTYGFPMDLTADVCRERNIIVDE
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCEEEEC
AGFEVAMAEQRSRAQAAGNFGADYNAALKIDAETAFSGYTELAGQAKVTAIYQNGESVTA
CCCEEHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHCCHHHHCCCEEEEEEEECCCEEEE
IKAGDEAVVVLDVTPFYAESGGQVGDKGQLVANGVEFTVNDTQKYGQATGHQGVLATGNL
EECCCCEEEEEECCCHHHCCCCCCCCCCCEEECCEEEEECCHHHHCCCCCCCCEEEECCC
SVGQVVEAKVDKKLRHRTQLNHSVTHLLHAALRQVLGTHVSQKGSLVDPERLRFDFSHFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHCC
GVKATELKEVEELVNTQIRRNHELKTAEMGIDEAKEKGAMALFGEKYDSQVRVVTMGDFS
CCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHCCCEEEECCCCCCEEEEEEECCEE
IELCGGTHVGRTGDIGLFKITSEAGIAAGVRRIEAVTGAAAMAYVAQQQAELEEAAALLK
EEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDANSVVAKLKAQLDKMKQLEKEMAQLKDKLAAAASADLVGDAVVVNGVNVLIKKLDGVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
ASSLRGLQDELKQKLKSAIIVLGTAQEGKVNLIAGVSNDLIGKVKAGELVAMVAAQVGGK
HHHHCCHHHHHHHHHHCEEEEEECCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHCCC
GGGRPDMAQAGGSQPENLDAALAQVLPWITERLA
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA