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The map label for this gene is phrA [H]

Identifier: 160876317

GI number: 160876317

Start: 3779455

End: 3780954

Strand: Reverse

Name: phrA [H]

Synonym: Sbal195_3211

Alternate gene names: 160876317

Gene position: 3780954-3779455 (Counterclockwise)

Preceding gene: 160876318

Following gene: 160876316

Centisome position: 70.71

GC content: 49.27

Gene sequence:

>1500_bases
ATGACATCAGTTAACGATTCAATGCCAGTAGATTCAATGCGAATAGAAGTGCAGGGCAATAATGCACTGATGTGGTTTCG
CCAAGATTTACGCCTTGCCGATAATCAGGCGCTAAGTGCAGCCTGTGATTGGGCGCGGGAGAATGGCGTGGCACTTAAGG
CCATTTATATCGCTACGCCAACTCAGTGGCAACGCCATGATGTGGCGCCCATTCAGTTAGATTTTATCGAACGTCACATC
AATCTGCTTGCCCAAGGGCTGGCGAGTTTGGGGATTACATTCGAATTAATTCAGTTAGATACCTTTGCCGATGTGCCAGC
GTTTATCAGTGATTACTGCCAGCAGCAAGGTATTAGCCGCGTGTTTGCAGGCAGTGAGCCTGAGATTAACGAGCAACAGC
GTGACCAAGCCTGTATTCAGGCTGGTATTCCTCTGGTGCTAACCGAAGAGCATTGTTTGCTTAAGCCGGGCACAGTGCTG
AACTTGTCCGGCGACATGTATAAGGTTTTCACCCCTTTTAGCCGTAAGTGGCGAGAAATTGCCGCCCGCCATGCCATTTT
GCCGCTTCCAGTTCCGGCGCCGCTTGGACCTGTGTTGACCGAGCCAAAGCCGCATTTATTTAACAATGTCACTAAAGTAT
CGAGTGAACTTTGGGCTGCGGGAGAAGGGCAGGCAAAGCGTATACTTTCAGCATTTATTCAGCAGAAAGTACAGGATTAT
AAGCAAGATAGGGATTTCCCCGCCATCGATGGCACCAGTTCAATTTCACCTTATCTGGCCATTGGCGTCGTTAGCCCAAG
ACAATGCGTTGCCGCGTTGCTGCATGACTTCCCCGAGGTGATTGTCGACGATACTAGCCCAGCTCGTACTTGGTTAAATG
AGCTTACCTGGCGTGAGTTTTATCGTCATTTGCTGGTGGCATTTCCTGACTTATCTAAGAATTATAATTTCAATAGACAA
GCCGACCATGTGCAGTGGCGTAACAATCAGCAGGAGTTTGCCGCTTGGTGTGAAGGTCGTACAGGCTATCCGATTGTCGA
TGCGGCAATGCGCCAGCTCAATCAAACGGGTTGGATGCACAATCGTTTGCGTATGGTGGTGGCGAGTTTCCTGACGAAAC
ATCTACTGATTGATTGGCGTTGGGGCGAGCGTAATTTCCGTCAAAAGCTTATCGATGGTGACTTAGCCGCGAATAATGGC
GGCTGGCAGTGGTCCGCCGGTTGTGGCTGTGATGCCCAGCCTTATTTTCGCATTTTTAATCCTATGAGCCAGAGTGAAAA
GTTTGATCCAGATGGCAGCTTCATCCGTAAGTACTTACCAGAGCTCGCATCTTGGGGAATTAAGCAATTACATCAACCCA
GTACAGCGCAGACACCGTCCTTGTTTGACCAGCCGTTATTCACTAAAACGGCTTATCCGAGTGCGATTGTCGAGCACAGT
GCAGCAAGAGCAAGGGCATTAACCGTATTAGGCGTATTGAAAAAGGCCGCGGCCCAATAA

Upstream 100 bases:

>100_bases
ATACCGTCTCGCTCCGTGGGTAAGGTCAACAGAAGTCACAAAGGCAAAGGGAAAAGTATTGGCGTGAGTCAAAGCGAGCA
GGGCAATAGTGGAGAAAACA

Downstream 100 bases:

>100_bases
ATAGGATCAGGAGCAATAACCAGATGACAAACCAAGCGACGACTGAGACTAATACGCTTGTTGATGATTTTATTCAGCTT
TATCAAGCCTTGAATAAAGA

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTSVNDSMPVDSMRIEVQGNNALMWFRQDLRLADNQALSAACDWARENGVALKAIYIATPTQWQRHDVAPIQLDFIERHI
NLLAQGLASLGITFELIQLDTFADVPAFISDYCQQQGISRVFAGSEPEINEQQRDQACIQAGIPLVLTEEHCLLKPGTVL
NLSGDMYKVFTPFSRKWREIAARHAILPLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQKVQDY
KQDRDFPAIDGTSSISPYLAIGVVSPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREFYRHLLVAFPDLSKNYNFNRQ
ADHVQWRNNQQEFAAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERNFRQKLIDGDLAANNG
GWQWSAGCGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFDQPLFTKTAYPSAIVEHS
AARARALTVLGVLKKAAAQ

Sequences:

>Translated_499_residues
MTSVNDSMPVDSMRIEVQGNNALMWFRQDLRLADNQALSAACDWARENGVALKAIYIATPTQWQRHDVAPIQLDFIERHI
NLLAQGLASLGITFELIQLDTFADVPAFISDYCQQQGISRVFAGSEPEINEQQRDQACIQAGIPLVLTEEHCLLKPGTVL
NLSGDMYKVFTPFSRKWREIAARHAILPLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQKVQDY
KQDRDFPAIDGTSSISPYLAIGVVSPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREFYRHLLVAFPDLSKNYNFNRQ
ADHVQWRNNQQEFAAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERNFRQKLIDGDLAANNG
GWQWSAGCGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFDQPLFTKTAYPSAIVEHS
AARARALTVLGVLKKAAAQ
>Mature_498_residues
TSVNDSMPVDSMRIEVQGNNALMWFRQDLRLADNQALSAACDWARENGVALKAIYIATPTQWQRHDVAPIQLDFIERHIN
LLAQGLASLGITFELIQLDTFADVPAFISDYCQQQGISRVFAGSEPEINEQQRDQACIQAGIPLVLTEEHCLLKPGTVLN
LSGDMYKVFTPFSRKWREIAARHAILPLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQKVQDYK
QDRDFPAIDGTSSISPYLAIGVVSPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREFYRHLLVAFPDLSKNYNFNRQA
DHVQWRNNQQEFAAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERNFRQKLIDGDLAANNGG
WQWSAGCGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFDQPLFTKTAYPSAIVEHSA
ARARALTVLGVLKKAAAQ

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=490, Percent_Identity=26.1224489795918, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI188536100, Length=491, Percent_Identity=25.4582484725051, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI188536103, Length=440, Percent_Identity=25.4545454545455, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1786926, Length=478, Percent_Identity=44.3514644351464, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324962, Length=503, Percent_Identity=32.4055666003976, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137248, Length=515, Percent_Identity=26.9902912621359, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24585455, Length=515, Percent_Identity=26.9902912621359, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24648152, Length=532, Percent_Identity=25.3759398496241, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 56303; Mature: 56171

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVNDSMPVDSMRIEVQGNNALMWFRQDLRLADNQALSAACDWARENGVALKAIYIATP
CCCCCCCCCCCCEEEEEECCCEEEEEHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEECC
TQWQRHDVAPIQLDFIERHINLLAQGLASLGITFELIQLDTFADVPAFISDYCQQQGISR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHCCHHHHHHHHHHHCCHH
VFAGSEPEINEQQRDQACIQAGIPLVLTEEHCLLKPGTVLNLSGDMYKVFTPFSRKWREI
HHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEECCCCEEECCCCEEEECCHHHHHHHHH
AARHAILPLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQKVQDY
HHHCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KQDRDFPAIDGTSSISPYLAIGVVSPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREF
HHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHH
YRHLLVAFPDLSKNYNFNRQADHVQWRNNQQEFAAWCEGRTGYPIVDAAMRQLNQTGWMH
HHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHH
NRLRMVVASFLTKHLLIDWRWGERNFRQKLIDGDLAANNGGWQWSAGCGCDAQPYFRIFN
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHC
PMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFDQPLFTKTAYPSAIVEHS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHCCCCHHHHHHH
AARARALTVLGVLKKAAAQ
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSVNDSMPVDSMRIEVQGNNALMWFRQDLRLADNQALSAACDWARENGVALKAIYIATP
CCCCCCCCCCCEEEEEECCCEEEEEHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEECC
TQWQRHDVAPIQLDFIERHINLLAQGLASLGITFELIQLDTFADVPAFISDYCQQQGISR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHCCHHHHHHHHHHHCCHH
VFAGSEPEINEQQRDQACIQAGIPLVLTEEHCLLKPGTVLNLSGDMYKVFTPFSRKWREI
HHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEECCCCEEECCCCEEEECCHHHHHHHHH
AARHAILPLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQKVQDY
HHHCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KQDRDFPAIDGTSSISPYLAIGVVSPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREF
HHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHH
YRHLLVAFPDLSKNYNFNRQADHVQWRNNQQEFAAWCEGRTGYPIVDAAMRQLNQTGWMH
HHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHH
NRLRMVVASFLTKHLLIDWRWGERNFRQKLIDGDLAANNGGWQWSAGCGCDAQPYFRIFN
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHC
PMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFDQPLFTKTAYPSAIVEHS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHCCCCHHHHHHH
AARARALTVLGVLKKAAAQ
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]