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The map label for this gene is yafC [C]

Identifier: 160876297

GI number: 160876297

Start: 3762927

End: 3763868

Strand: Reverse

Name: yafC [C]

Synonym: Sbal195_3191

Alternate gene names: 160876297

Gene position: 3763868-3762927 (Counterclockwise)

Preceding gene: 160876298

Following gene: 160876288

Centisome position: 70.39

GC content: 46.71

Gene sequence:

>942_bases
ATGTTAGAACTGTTAGAACCTATTGCGATTTTTACTCATGTGGCCAGAGCTGGTAGTTTTAGTGCCGCCGCAAGAAGGCT
TGGGATCTCTAAGTCTAAGGTCAGTACACAAGTCGCCGATCTTGAACATAGACTTGGTGTTCAATTAATCCAACGTACCA
CCCGTAGCTTAAGCCTGACTGAAGCAGGGCATTTGCTGTATCTGCAAGGTGAAGAACTGCTGCGTGACGCTGAACAAGCG
ATAGCCAGCGTGCATAACTTAAATGATGCGACCCGCGGTGTGCTCAAGGTGGGGATTTCCCAATCCTTTGGGGTGATGCA
TATCATTCCTGCGCTGCCATCCTTTATGGCAAAACATCCTGAGCTTGAGCTACAAGTGAGCTTACTGGACCATAAAGTAG
ATGTAGTGAGTGAAGGCTTAGATTTATTGTTAACTATGTCGGAGCAATTACCGCTTGGCATGGTTGCTAGACCTCTGATG
AAGTGCCAATTCCTGTTAGTTGCTTCTCCAGGCTACATTGCTCAACATGGCGAACCAACTCGACCTGAGCAGTTAGTTGA
CCACAATTGTTTGGTCTATCAAGGCGAGTGGCATGAGCACAGCATGTGGCAATTTAAGAAGGGCGATGATTACTGTGAAA
TTGGCGTATCGGGCAATTTCAGGGTGGATAACGCCCCAGCATTAAAATCCGCCGCAATCAGTGGTCTTGGGGTTGTGTAT
CTTGCAAGTTATCTGATGGAAGATGAAATTGCCAAAGGGACATTAGTGCCTTTACTGAAGGATTGGCAATTGACCCACCA
TCTGCCATTGCAGGCGGTTTATCCCCGTCGTAAGCACTTAGCACCTAAGGTCAGTTCTTTTATCGAGTTCATCAAAGATC
ACATAGGAACGCCGCCGTATTGGGATGCGCCCTATGTCGAATTATTCAGTAAACGTCAGTAA

Upstream 100 bases:

>100_bases
AGATATCAATGCTATGGTTATTTTTAAGCGATGATTACGCTAACCACTGCTACTCACCGAAAGTTAATATCATTGTCGTA
AACAGCTTAAGGCGACTTCC

Downstream 100 bases:

>100_bases
AATAGGAAACATTGTTCTCAAAACCAGTTCTATGTGATTTCAAATTAACCAAGTACTTTTATGGCTATTTAAAATCAATA
CTTTAGATTGAGTTGATGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQA
IASVHNLNDATRGVLKVGISQSFGVMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLVASPGYIAQHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
LASYLMEDEIAKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSSFIEFIKDHIGTPPYWDAPYVELFSKRQ

Sequences:

>Translated_313_residues
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQA
IASVHNLNDATRGVLKVGISQSFGVMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLVASPGYIAQHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
LASYLMEDEIAKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSSFIEFIKDHIGTPPYWDAPYVELFSKRQ
>Mature_313_residues
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQA
IASVHNLNDATRGVLKVGISQSFGVMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLVASPGYIAQHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
LASYLMEDEIAKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSSFIEFIKDHIGTPPYWDAPYVELFSKRQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=30.6397306397306, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=29.7945205479452, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1789440, Length=299, Percent_Identity=26.4214046822742, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=27.4914089347079, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=31.1188811188811, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1789639, Length=301, Percent_Identity=27.2425249169435, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=26.2975778546713, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788706, Length=306, Percent_Identity=26.1437908496732, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788297, Length=303, Percent_Identity=25.7425742574257, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=295, Percent_Identity=24.7457627118644, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788481, Length=320, Percent_Identity=27.8125, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=33.6134453781513, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=26.4227642276423, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790783, Length=293, Percent_Identity=24.2320819112628, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=26.6272189349112, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=121, Percent_Identity=33.8842975206612, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1787601, Length=188, Percent_Identity=26.063829787234, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34854; Mature: 34854

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
EAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGVMHIIPALPSFMAKHP
CCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
ELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPGYIAQHGEPT
CCEEEEHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCC
RPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
CHHHHHCCCEEEEECCCCCCCCEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LASYLMEDEIAKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSSFIEFIKDHIGTPPY
HHHHHHHHHHCCCCHHHHHHCCCHHHCCCCHHCCCHHHHCCHHHHHHHHHHHHHCCCCCC
WDAPYVELFSKRQ
CCCHHHHHHCCCC
>Mature Secondary Structure
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
EAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGVMHIIPALPSFMAKHP
CCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
ELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPGYIAQHGEPT
CCEEEEHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCC
RPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
CHHHHHCCCEEEEECCCCCCCCEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LASYLMEDEIAKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSSFIEFIKDHIGTPPY
HHHHHHHHHHCCCCHHHHHHCCCHHHCCCCHHCCCHHHHCCHHHHHHHHHHHHHCCCCCC
WDAPYVELFSKRQ
CCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]