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The map label for this gene is acbB [H]

Identifier: 160876223

GI number: 160876223

Start: 3680159

End: 3681139

Strand: Direct

Name: acbB [H]

Synonym: Sbal195_3117

Alternate gene names: 160876223

Gene position: 3680159-3681139 (Clockwise)

Preceding gene: 160876222

Following gene: 160876224

Centisome position: 68.82

GC content: 42.51

Gene sequence:

>981_bases
ATGAAAAAAGTACTAGTGACTGGAGCGGATGGGTTTATCGGCTCTCACCTTGTCGAAATGCTAGTTGCACAAGGTTATCA
AGTAAGGGCACTTAGCCAATATAACTCCTTCAATTATTGGGGTTGGCTTGAAAACATTGATTGTCTCGATGAGGTTGAAG
TCATTTGTGGAGATATTCGTGATCCGCACTTCTGTAAACACTTATGCAAAGATATCGATGTGATTTATCACCTTGCAGCG
CTTATCGCAATTCCTTATTCCTATATAGCCCCCGATAGCTATCTTGATACCAACGCGAAAGGCACCCTCAATATTTGTCA
GGCCGCACTTGAAAATAACGTTTCAAGAGTGATTCACACCTCAACTAGTGAAGTCTACGGCACAGCTAAATACGTCCCCA
TTGATGAACAACACCCTCTGCAGCCACAGTCACCTTATAGTGCCTCAAAAATGGCGGCAGATGCGATGGCAATGAGTTTT
CACAATAGCTTTGAGTTACCGCTCACTATCGCTCGACCATTTAATACTTATGGTCCGAGGCAATCGGCACGAGCTGTGAT
CCCGACGATTATTAGCCAAATTGCTGCGGGAGCGACACAAATCAAGTTAGGCGATATCTCCCCTACCCGTGATTTCAACT
ACGTGTTAGATACTTGCCGTGGCTTCATCGCATTGGCGGCGCATGACAATTGCATCGGTGAAACGCTCAATATCTCGTCC
AATTATGAAATATCAATTGAAGACACTCTGAACATTATCAAGCAAAACATGCATAGTGATGTGGAATTCATTACGGACGA
CGCACGCTTAAGACCTCAACAATCTGAAGTGTTCAGATTATGGGGAGATAATAGTAAAATCAAAACACTAACTGGATACC
AACCTCAATTCGACATTCACATAGGATTGAAAGAAACCATTACTTGGTTCACCGAACCAGCTAATCTGAGCAAATATAAG
TCTACTATTTACAATAGATAA

Upstream 100 bases:

>100_bases
CTTAATGTTGAATTATACGACTTCATTCAACAATTCGACGATTCTAGAGATTAGTTGAAACGCTAGGCCCTGATGCCTCT
TTCAAAAGCAAAGGATTATC

Downstream 100 bases:

>100_bases
TAGGAGTGTGGCATCAATGACGGCATCATGGGATCCAGTATGGGAAACAGTATTCACAACCCAAGCTTGGGGAAAATACC
CAGGTGAAGACTTAATTCGA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKKVLVTGADGFIGSHLVEMLVAQGYQVRALSQYNSFNYWGWLENIDCLDEVEVICGDIRDPHFCKHLCKDIDVIYHLAA
LIAIPYSYIAPDSYLDTNAKGTLNICQAALENNVSRVIHTSTSEVYGTAKYVPIDEQHPLQPQSPYSASKMAADAMAMSF
HNSFELPLTIARPFNTYGPRQSARAVIPTIISQIAAGATQIKLGDISPTRDFNYVLDTCRGFIALAAHDNCIGETLNISS
NYEISIEDTLNIIKQNMHSDVEFITDDARLRPQQSEVFRLWGDNSKIKTLTGYQPQFDIHIGLKETITWFTEPANLSKYK
STIYNR

Sequences:

>Translated_326_residues
MKKVLVTGADGFIGSHLVEMLVAQGYQVRALSQYNSFNYWGWLENIDCLDEVEVICGDIRDPHFCKHLCKDIDVIYHLAA
LIAIPYSYIAPDSYLDTNAKGTLNICQAALENNVSRVIHTSTSEVYGTAKYVPIDEQHPLQPQSPYSASKMAADAMAMSF
HNSFELPLTIARPFNTYGPRQSARAVIPTIISQIAAGATQIKLGDISPTRDFNYVLDTCRGFIALAAHDNCIGETLNISS
NYEISIEDTLNIIKQNMHSDVEFITDDARLRPQQSEVFRLWGDNSKIKTLTGYQPQFDIHIGLKETITWFTEPANLSKYK
STIYNR
>Mature_326_residues
MKKVLVTGADGFIGSHLVEMLVAQGYQVRALSQYNSFNYWGWLENIDCLDEVEVICGDIRDPHFCKHLCKDIDVIYHLAA
LIAIPYSYIAPDSYLDTNAKGTLNICQAALENNVSRVIHTSTSEVYGTAKYVPIDEQHPLQPQSPYSASKMAADAMAMSF
HNSFELPLTIARPFNTYGPRQSARAVIPTIISQIAAGATQIKLGDISPTRDFNYVLDTCRGFIALAAHDNCIGETLNISS
NYEISIEDTLNIIKQNMHSDVEFITDDARLRPQQSEVFRLWGDNSKIKTLTGYQPQFDIHIGLKETITWFTEPANLSKYK
STIYNR

Specific function: Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4- hexulose via a three-step process involving oxidation, dehydration and re

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=32.398753894081, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI42516563, Length=325, Percent_Identity=28.9230769230769, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4504031, Length=327, Percent_Identity=25.0764525993884, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI56237023, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI56118217, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI189083684, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4504507, Length=187, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260763931, Length=187, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504509, Length=187, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI193211614, Length=239, Percent_Identity=25.1046025104602, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI8393516, Length=239, Percent_Identity=25.1046025104602, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI48994969, Length=359, Percent_Identity=27.8551532033426, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788353, Length=339, Percent_Identity=28.6135693215339, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788366, Length=361, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786974, Length=311, Percent_Identity=27.9742765273312, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=320, Percent_Identity=28.75, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=26.1398176291793, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=340, Percent_Identity=23.8235294117647, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=345, Percent_Identity=24.0579710144928, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71987463, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=23.3333333333333, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=258, Percent_Identity=29.0697674418605, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356223, Length=324, Percent_Identity=29.9382716049383, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19923002, Length=347, Percent_Identity=24.7838616714697, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24158427, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36557; Mature: 36557

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLVTGADGFIGSHLVEMLVAQGYQVRALSQYNSFNYWGWLENIDCLDEVEVICGDIR
CCEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHCCCCCEEECCCCCCHHHHHHHHHCCCC
DPHFCKHLCKDIDVIYHLAALIAIPYSYIAPDSYLDTNAKGTLNICQAALENNVSRVIHT
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEC
STSEVYGTAKYVPIDEQHPLQPQSPYSASKMAADAMAMSFHNSFELPLTIARPFNTYGPR
CCHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
QSARAVIPTIISQIAAGATQIKLGDISPTRDFNYVLDTCRGFIALAAHDNCIGETLNISS
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCEEECCC
NYEISIEDTLNIIKQNMHSDVEFITDDARLRPQQSEVFRLWGDNSKIKTLTGYQPQFDIH
CEEEEHHHHHHHHHHHHHCCHHEEECCCCCCCCHHHEEEEECCCCCEEEEECCCCCEEEE
IGLKETITWFTEPANLSKYKSTIYNR
ECHHHHHHEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKKVLVTGADGFIGSHLVEMLVAQGYQVRALSQYNSFNYWGWLENIDCLDEVEVICGDIR
CCEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHCCCCCEEECCCCCCHHHHHHHHHCCCC
DPHFCKHLCKDIDVIYHLAALIAIPYSYIAPDSYLDTNAKGTLNICQAALENNVSRVIHT
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEC
STSEVYGTAKYVPIDEQHPLQPQSPYSASKMAADAMAMSFHNSFELPLTIARPFNTYGPR
CCHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
QSARAVIPTIISQIAAGATQIKLGDISPTRDFNYVLDTCRGFIALAAHDNCIGETLNISS
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCEEECCC
NYEISIEDTLNIIKQNMHSDVEFITDDARLRPQQSEVFRLWGDNSKIKTLTGYQPQFDIH
CEEEEHHHHHHHHHHHHHCCHHEEECCCCCCCCHHHEEEEECCCCCEEEEECCCCCEEEE
IGLKETITWFTEPANLSKYKSTIYNR
ECHHHHHHEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10196166 [H]