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The map label for this gene is mlaA [H]

Identifier: 160876148

GI number: 160876148

Start: 3610489

End: 3611274

Strand: Reverse

Name: mlaA [H]

Synonym: Sbal195_3039

Alternate gene names: 160876148

Gene position: 3611274-3610489 (Counterclockwise)

Preceding gene: 160876151

Following gene: 160876147

Centisome position: 67.53

GC content: 43.51

Gene sequence:

>786_bases
ATGAAGTTAAAATGGATGGGATTATCTTTGGGGTTAATGCTGTTGCCTAAAGTGCAAGCGGCTGAAGTGCCCGTATCAGA
TACCATTCAGCAGGAAGCACCTGCTAAGGTACAAATCAGCTATGATGATCCCCGCGACCCATTCGAAGGGTTTAACCGTG
CAATGTGGGATTTTAACTATCTCTTTTTAGATAGATATTTATATCGTCCTGTTGCCCATGGTTATAACGATTATATTCCA
ATGCCAGCTAAAACAGGCGTCAATAATTTTGTACAGAATTTAGAAGAACCTAGCAGCTTGGTAAACAACGTGCTGCAAGG
AAAGTGGGGCTGGGCGGCGAATGCCGGTGGACGTTTTACCATCAACTCTACAGTCGGTTTATTAGGAGTCATTGACGTCG
CAGATATGATGGGTATGAGCCGTAAGCAAGATGAGTTTAACGAAGTGCTTGGTTATTATGGCGTGCCCAACGGACCTTAC
TTCATGGCGCCGTTTGCGGGGCCTTATGTCGTGCGAGAACTCGCGTCTGATTGGGTTGATGGTCTATACTTTCCCTTGTC
TGAGCTAACAATCTGGCAAACCATTGTTAAATGGGGACTTAAAAATCTTCATTCCAGAGCGTCTGCGATAGATCAAGAAC
GACTCGTTGATAATGCACTCGATCCTTATGCCTTTGTCAAAGATGCGTATTTACAACACATGGACTATAAAGTCTATGAT
GGTAATGTTCCTCAAAAACAAGATGATGATGAGTTACTCGATCAGTATATGCAGGAGCTTGAGTAA

Upstream 100 bases:

>100_bases
GTATGCCACTAAGACGAATTATCTTTATATCGTCATTTTTGTATTGTCACTTTAGCGAAAACTTGATGCCTAAGCTGTAC
ATTTATATTAAGAGAGCGAT

Downstream 100 bases:

>100_bases
CCCTATAGGCCGTACTTGACAGCATTTGTCGTTAGTATTCAAGTGTTAGCCTTATCGATAAACGTTATCGGTATATTCAC
TTGAGTAATGGCAATGCGAT

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKLKWMGLSLGLMLLPKVQAAEVPVSDTIQQEAPAKVQISYDDPRDPFEGFNRAMWDFNYLFLDRYLYRPVAHGYNDYIP
MPAKTGVNNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTINSTVGLLGVIDVADMMGMSRKQDEFNEVLGYYGVPNGPY
FMAPFAGPYVVRELASDWVDGLYFPLSELTIWQTIVKWGLKNLHSRASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYD
GNVPQKQDDDELLDQYMQELE

Sequences:

>Translated_261_residues
MKLKWMGLSLGLMLLPKVQAAEVPVSDTIQQEAPAKVQISYDDPRDPFEGFNRAMWDFNYLFLDRYLYRPVAHGYNDYIP
MPAKTGVNNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTINSTVGLLGVIDVADMMGMSRKQDEFNEVLGYYGVPNGPY
FMAPFAGPYVVRELASDWVDGLYFPLSELTIWQTIVKWGLKNLHSRASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYD
GNVPQKQDDDELLDQYMQELE
>Mature_261_residues
MKLKWMGLSLGLMLLPKVQAAEVPVSDTIQQEAPAKVQISYDDPRDPFEGFNRAMWDFNYLFLDRYLYRPVAHGYNDYIP
MPAKTGVNNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTINSTVGLLGVIDVADMMGMSRKQDEFNEVLGYYGVPNGPY
FMAPFAGPYVVRELASDWVDGLYFPLSELTIWQTIVKWGLKNLHSRASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYD
GNVPQKQDDDELLDQYMQELE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=265, Percent_Identity=36.6037735849057, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29760; Mature: 29760

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKWMGLSLGLMLLPKVQAAEVPVSDTIQQEAPAKVQISYDDPRDPFEGFNRAMWDFNY
CCEEEECHHHHHHHHCCCHHHCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
LFLDRYLYRPVAHGYNDYIPMPAKTGVNNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFT
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEE
INSTVGLLGVIDVADMMGMSRKQDEFNEVLGYYGVPNGPYFMAPFAGPYVVRELASDWVD
ECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHH
GLYFPLSELTIWQTIVKWGLKNLHSRASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEC
GNVPQKQDDDELLDQYMQELE
CCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKWMGLSLGLMLLPKVQAAEVPVSDTIQQEAPAKVQISYDDPRDPFEGFNRAMWDFNY
CCEEEECHHHHHHHHCCCHHHCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
LFLDRYLYRPVAHGYNDYIPMPAKTGVNNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFT
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEE
INSTVGLLGVIDVADMMGMSRKQDEFNEVLGYYGVPNGPYFMAPFAGPYVVRELASDWVD
ECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHH
GLYFPLSELTIWQTIVKWGLKNLHSRASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEC
GNVPQKQDDDELLDQYMQELE
CCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]