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The map label for this gene is rffG [H]

Identifier: 160876126

GI number: 160876126

Start: 3586495

End: 3587517

Strand: Reverse

Name: rffG [H]

Synonym: Sbal195_3017

Alternate gene names: 160876126

Gene position: 3587517-3586495 (Counterclockwise)

Preceding gene: 160876127

Following gene: 160876123

Centisome position: 67.09

GC content: 44.77

Gene sequence:

>1023_bases
ATGCGAGTGCTCGTTACCGGTGGTGCAGGCTTTATTGGTTCTGCTTTGGTGCGTATGCTGATTGAGCAAACGACGTGTGT
TGTGATCAATTTCGATAAGTTGACCTATGCCAGTGATCTCGAGTCCTTAGCATCTATTGCCGATAGTGAGCGCTATCATT
TTATTCAGGCAGATATTGGCGATAGGGCGAAATTAGATCAAGTGTTTCAGGATTATCTGCCAGATGTGGTGATGCATTTG
GCGGCCGAAAGCCATGTTGATCGCTCAATTAATGGTCCTGCTGAATTTATCCAAACTAATATAGTGGGTACTTATACATT
GCTTGAGGCGTGCCGCTGTTATTTCCAAAGTTTGAATACGGATAAGCAAAAAGTATTTAGATTCCATCATATTTCTACCG
ATGAGGTCTACGGTTCACTTGGTGATACGGGGCTCTTTTCTGAGACTACAGCTTATGATCCCAGTTCTCCTTATTCGGCG
AGCAAGGCATCGGCGGACCATCTTGTTAGAGCTTGGCATCGCACCTATGGTTTACCGATTGTCATCACTAACTGCTCTAA
CAATTATGGGCCTTTTCAATATCCTGAGAAATTGATCCCGTTAATGGTGTTGAATGCATTAGCTGGCAAGCAGTTACCTG
TATATGGCAATGGTCAACAAGTTCGTGATTGGTTGTACGTTGATGACCATGTTCGGGCGTTGTTTCTCGTGGTGACCCAG
GGCACAGTGGGGGAGACTTATAATATTGGTGGCACTAATGAGCGGAGTAATCTTGAAGTCGTACATCAAATATGCGATTT
ACTTGAGGAGTTAGTACCCACTCATGCACAAGCACTTGCGGCTGATGGTGTTGGTTTTCGCAGTTTGGTAGAACATGTTA
CTGACAGAGCAGGGCATGACGTTCGCTATGCTATTGATGCTAGCAAGATCCAGCGAGAGCTTGGTTGGCAACCATTAGAA
TCCTTTGATTCAGGCCTTAGGAAAACTGTCGAGTGGATAGTGGCTCGGTATCATAAATCGTAA

Upstream 100 bases:

>100_bases
ATGTCCTTTCGAATATTATTATGCTGATTTTTTCAAGCCAGAAAGTTGTATACTTTCTGGCTTTTTTATTTTAATGCTTT
TTATTTTTGGGGTTGGCGAT

Downstream 100 bases:

>100_bases
ATTGATTAATATTGCAGATTTGTACCGCTAGCGAGCATGGGGAAATTTGAGGTTACATATTATTTGAAGCTACTTATTCA
GTGCAGTTGAAGCTAAGACA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRVLVTGGAGFIGSALVRMLIEQTTCVVINFDKLTYASDLESLASIADSERYHFIQADIGDRAKLDQVFQDYLPDVVMHL
AAESHVDRSINGPAEFIQTNIVGTYTLLEACRCYFQSLNTDKQKVFRFHHISTDEVYGSLGDTGLFSETTAYDPSSPYSA
SKASADHLVRAWHRTYGLPIVITNCSNNYGPFQYPEKLIPLMVLNALAGKQLPVYGNGQQVRDWLYVDDHVRALFLVVTQ
GTVGETYNIGGTNERSNLEVVHQICDLLEELVPTHAQALAADGVGFRSLVEHVTDRAGHDVRYAIDASKIQRELGWQPLE
SFDSGLRKTVEWIVARYHKS

Sequences:

>Translated_340_residues
MRVLVTGGAGFIGSALVRMLIEQTTCVVINFDKLTYASDLESLASIADSERYHFIQADIGDRAKLDQVFQDYLPDVVMHL
AAESHVDRSINGPAEFIQTNIVGTYTLLEACRCYFQSLNTDKQKVFRFHHISTDEVYGSLGDTGLFSETTAYDPSSPYSA
SKASADHLVRAWHRTYGLPIVITNCSNNYGPFQYPEKLIPLMVLNALAGKQLPVYGNGQQVRDWLYVDDHVRALFLVVTQ
GTVGETYNIGGTNERSNLEVVHQICDLLEELVPTHAQALAADGVGFRSLVEHVTDRAGHDVRYAIDASKIQRELGWQPLE
SFDSGLRKTVEWIVARYHKS
>Mature_340_residues
MRVLVTGGAGFIGSALVRMLIEQTTCVVINFDKLTYASDLESLASIADSERYHFIQADIGDRAKLDQVFQDYLPDVVMHL
AAESHVDRSINGPAEFIQTNIVGTYTLLEACRCYFQSLNTDKQKVFRFHHISTDEVYGSLGDTGLFSETTAYDPSSPYSA
SKASADHLVRAWHRTYGLPIVITNCSNNYGPFQYPEKLIPLMVLNALAGKQLPVYGNGQQVRDWLYVDDHVRALFLVVTQ
GTVGETYNIGGTNERSNLEVVHQICDLLEELVPTHAQALAADGVGFRSLVEHVTDRAGHDVRYAIDASKIQRELGWQPLE
SFDSGLRKTVEWIVARYHKS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=36.9822485207101, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI42516563, Length=344, Percent_Identity=25, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=25.6267409470752, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=25.6267409470752, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=25.6267409470752, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=68.0597014925373, Blast_Score=476, Evalue=1e-136,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=62.7906976744186, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=25.9154929577465, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788366, Length=246, Percent_Identity=27.6422764227642, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=38.6904761904762, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=35.6287425149701, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71982035, Length=350, Percent_Identity=27.1428571428571, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=351, Percent_Identity=24.2165242165242, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=352, Percent_Identity=26.9886363636364, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=252, Percent_Identity=26.5873015873016, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=27.1978021978022, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=23.9766081871345, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=25.6267409470752, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24158427, Length=334, Percent_Identity=24.251497005988, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37921; Mature: 37921

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVTGGAGFIGSALVRMLIEQTTCVVINFDKLTYASDLESLASIADSERYHFIQADIG
CEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCC
DRAKLDQVFQDYLPDVVMHLAAESHVDRSINGPAEFIQTNIVGTYTLLEACRCYFQSLNT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKQKVFRFHHISTDEVYGSLGDTGLFSETTAYDPSSPYSASKASADHLVRAWHRTYGLPI
CHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VITNCSNNYGPFQYPEKLIPLMVLNALAGKQLPVYGNGQQVRDWLYVDDHVRALFLVVTQ
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEECHHHHEEEEEEEC
GTVGETYNIGGTNERSNLEVVHQICDLLEELVPTHAQALAADGVGFRSLVEHVTDRAGHD
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
VRYAIDASKIQRELGWQPLESFDSGLRKTVEWIVARYHKS
EEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLVTGGAGFIGSALVRMLIEQTTCVVINFDKLTYASDLESLASIADSERYHFIQADIG
CEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCC
DRAKLDQVFQDYLPDVVMHLAAESHVDRSINGPAEFIQTNIVGTYTLLEACRCYFQSLNT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKQKVFRFHHISTDEVYGSLGDTGLFSETTAYDPSSPYSASKASADHLVRAWHRTYGLPI
CHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VITNCSNNYGPFQYPEKLIPLMVLNALAGKQLPVYGNGQQVRDWLYVDDHVRALFLVVTQ
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEECHHHHEEEEEEEC
GTVGETYNIGGTNERSNLEVVHQICDLLEELVPTHAQALAADGVGFRSLVEHVTDRAGHD
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
VRYAIDASKIQRELGWQPLESFDSGLRKTVEWIVARYHKS
EEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]