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The map label for this gene is purB [H]

Identifier: 160875705

GI number: 160875705

Start: 3067252

End: 3068622

Strand: Direct

Name: purB [H]

Synonym: Sbal195_2593

Alternate gene names: 160875705

Gene position: 3067252-3068622 (Clockwise)

Preceding gene: 160875704

Following gene: 160875706

Centisome position: 57.36

GC content: 46.75

Gene sequence:

>1371_bases
ATGGATCTTTCCGCACTAACTGCTATCTCTCCGGTAGACGGTCGTTATGGTAGTAAAACCGCGTCATTGAGAGGGATTTT
CAGCGAGTTCGGTCTTACCAAGTACCGTGTTCAGGTTGAAATCAATTGGCTAAAACTGTTAGCAGACTGCCCAGATATTA
GCGAAGTGCCAACATTGAGCGCGTCAGCTATTGCTGTGCTCGATGGCATTAAAGACAATTTCAACGAACAAGATGCGCTG
CGTGTTAAGGCTATCGAAAGCACCACTAACCACGATGTGAAAGCGGTTGAATACTTCATCAAAGAAAAAATCGCGGGCAA
TAGCGAATTAGCCGCTGTGGGTGAGTTTGTTCACTTTGCCTGTACTTCCGAAGATATCAACAACTTAAGCCACGGTTTAA
TGTTGACTGGAGCCCGTGAGCAAGTATTACTGCCATATTGCAACGAGTTGCTGACTGCGATTAAAAAGCTGGCGATTGAA
TATCGCTCTGTGCCATTAATGTCACGCACCCATGGCCAACCTGCTTCACCTTCGACACTCGGTAAAGAGATGGCGAACGT
GGCGATGCGTCTTGAGCGTCAAATCAAACAAATTGCCAATGTTGAAATCATGGGCAAACTGAACGGTGCCGTAGGTAACT
ACAATGCTCACCTGTCAGCCTACCCTGAAGTTAATTGGCATGAACTGTCTGAGCGTTTTGTGACTAGCCTTGGCCTGCAC
TGGAACGCCTACACGACGCAAATCGAACCACACGATTACATTGCCGAACTGTTTGATGCGGTTGCCCGTTTCAACACTGT
GCTGATTGACTTTGACCGTGACATTTGGGGTTACATTGCCCTAGGTCACTTCAAACAACGCACTATCGCCGGTGAAATCG
GTTCATCGACTATGCCGCACAAAGTTAACCCAATCGATTTTGAAAACTCTGAGGGTAACTTAGGTATCGCTAACGCGCTG
ATGCAACATTTAGCGTCTAAGCTGCCGTTATCAAGATGGCAACGTGACTTAACTGACTCAACTGTGCTGCGTAACTTAGG
CGTAGGTATTGCCCATGCGTTGATCGCGTATCAAGCGACCTTAAAAGGCATCAGCAAGTTAGAAGTTAACGAAGCGCACC
TGCGTGATGAGTTAGATCACAACTGGGAAGTGTTAGCAGAACCCGTACAAACGGTAATGCGTCGTTACGGCATTGAAAAG
CCATACGAAAAATTAAAAGAACTGACGCGCGGTAAACGTATTGACGCGCAGCAACTGTCAGTGTTTATTGACGGACTTGA
ATTACCAGACTCAGTGAAAGCTGAACTGAAGAAAATGACGCCCGCCAATTACATTGGTCGCGCCGAAACGTTTGTCGACG
AACTGAAGTAA

Upstream 100 bases:

>100_bases
GTAAGCGTCGACACTTAGTCTTCGCCCGCAAAGCCATAGTCGATACAGCCAAGAAAAGTCTTACCCTATAATAAAATTAG
CGTTCATCAAGGAGCTTCAC

Downstream 100 bases:

>100_bases
GTTTTTAACTGAACTTAGTTTTTAAATAAACTTAGTTATTAGCTGAAATTGGCGATGGACAATACTCTTTCATAAACAAA
AATGAGGATTAAGGCGACTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLADCPDISEVPTLSASAIAVLDGIKDNFNEQDAL
RVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFACTSEDINNLSHGLMLTGAREQVLLPYCNELLTAIKKLAIE
YRSVPLMSRTHGQPASPSTLGKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHALIAYQATLKGISKLEVNEAHLRDELDHNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDAQQLSVFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK

Sequences:

>Translated_456_residues
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLADCPDISEVPTLSASAIAVLDGIKDNFNEQDAL
RVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFACTSEDINNLSHGLMLTGAREQVLLPYCNELLTAIKKLAIE
YRSVPLMSRTHGQPASPSTLGKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHALIAYQATLKGISKLEVNEAHLRDELDHNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDAQQLSVFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK
>Mature_456_residues
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLADCPDISEVPTLSASAIAVLDGIKDNFNEQDAL
RVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFACTSEDINNLSHGLMLTGAREQVLLPYCNELLTAIKKLAIE
YRSVPLMSRTHGQPASPSTLGKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHALIAYQATLKGISKLEVNEAHLRDELDHNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDAQQLSVFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=75.219298245614, Blast_Score=742, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50845; Mature: 50845

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLADCPDISEVPTLS
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCCC
ASAIAVLDGIKDNFNEQDALRVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFA
HHHHHHHHHHHHCCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CTSEDINNLSHGLMLTGAREQVLLPYCNELLTAIKKLAIEYRSVPLMSRTHGQPASPSTL
CCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHH
GKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
HHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPH
CCCEEECCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALMQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHALIAYQAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LKGISKLEVNEAHLRDELDHNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDAQQLS
HHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
VFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLADCPDISEVPTLS
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCCC
ASAIAVLDGIKDNFNEQDALRVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFA
HHHHHHHHHHHHCCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CTSEDINNLSHGLMLTGAREQVLLPYCNELLTAIKKLAIEYRSVPLMSRTHGQPASPSTL
CCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHH
GKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
HHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPH
CCCEEECCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALMQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHALIAYQAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LKGISKLEVNEAHLRDELDHNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDAQQLS
HHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
VFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]