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The map label for this gene is rluE [H]

Identifier: 160875701

GI number: 160875701

Start: 3063790

End: 3064638

Strand: Direct

Name: rluE [H]

Synonym: Sbal195_2589

Alternate gene names: 160875701

Gene position: 3063790-3064638 (Clockwise)

Preceding gene: 160875699

Following gene: 160875702

Centisome position: 57.3

GC content: 47.11

Gene sequence:

>849_bases
ATGACCACATTAACGACTCAATCAAGTCAGGTAAGCGTGTCTCAACCTCCTGTTAGCAAAAGTATGAACAAAAACAAGTT
TCCACCTAAACGTGCCAATTCCAGTACCAGTTCCCCAGCCAAGAGCACGATTAAACCCGCTAAGCCCGCGAGCAGAGGCC
CTGTCAACCGCGCTGCCGCCGTAGATAGCAAAGTGGCGCCGGCGCGTAATGCCAATGGCAGTTTAAAGAAAGAACCCTTT
AAAAATCCCAATCGACGCGCAATTGCTGCGGCAAAACCGGTGCAGAGCCAAACGATCGCACCGATTATCGTGCTATTTAA
TAAGCCTTTTGATGTACTTTGCCAGTTTACCGATGAACAAGGTCGTAAAACCCTAAAAGACTATATCCCCATTGCTGACG
TGTATGCCGCAGGCCGCTTAGACAGAGACAGTGAGGGCTTGTTGTTGCTGACCAATGACGGTCAATTACAGGCAAGACTG
ACAGAACCGAAAAAGAAAACCTTTAAAACCTATTGGGTGCAAGTAGAAGGCGTGCCGACGGAAGATGCACTGACGAAACT
GCGCCAAGGTGTTGAATTAAATGATGGTATGACGCTACCAGCTAAAGTCAAAATCATGGATTCACCCGATATTTGGCCAC
GTAATCCGCCGGTGAGAGAACGCAAAAATATCCCAACAACTTGGTTAGAAATCCAAATTCATGAAGGCCGTAACCGCCAA
GTCAGACGCATGACAGCGCATATCGGTTTTCCGACCTTAAGATTAATCCGTTATAAAATAGGCCAATGGAGTCTAGATAA
TTTGCCATCGGGCGAGCATAAATGCATCCGCCTCGGTGATGAAGTCTAA

Upstream 100 bases:

>100_bases
ACCTAATAAACATTTAGGCACAGATCACATTTTCTGGCCGACATCATAGAGCATTCGCAATAATATTCAAATTCCACTAA
CGGCGAATGCCAAGTACACT

Downstream 100 bases:

>100_bases
CTTTCAGCCAAGTTTCTACGCTCAACGCCACTAAAAAGTGGCACCATGTCCACTCAAAGGAGGCACTATGAGTCAAAGAT
ATAAACCCAATGTGACCGTC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTTLTTQSSQVSVSQPPVSKSMNKNKFPPKRANSSTSSPAKSTIKPAKPASRGPVNRAAAVDSKVAPARNANGSLKKEPF
KNPNRRAIAAAKPVQSQTIAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARL
TEPKKKTFKTYWVQVEGVPTEDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQ
VRRMTAHIGFPTLRLIRYKIGQWSLDNLPSGEHKCIRLGDEV

Sequences:

>Translated_282_residues
MTTLTTQSSQVSVSQPPVSKSMNKNKFPPKRANSSTSSPAKSTIKPAKPASRGPVNRAAAVDSKVAPARNANGSLKKEPF
KNPNRRAIAAAKPVQSQTIAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARL
TEPKKKTFKTYWVQVEGVPTEDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQ
VRRMTAHIGFPTLRLIRYKIGQWSLDNLPSGEHKCIRLGDEV
>Mature_281_residues
TTLTTQSSQVSVSQPPVSKSMNKNKFPPKRANSSTSSPAKSTIKPAKPASRGPVNRAAAVDSKVAPARNANGSLKKEPFK
NPNRRAIAAAKPVQSQTIAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARLT
EPKKKTFKTYWVQVEGVPTEDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQV
RRMTAHIGFPTLRLIRYKIGQWSLDNLPSGEHKCIRLGDEV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=69.7142857142857, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1787524, Length=173, Percent_Identity=34.1040462427746, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788510, Length=185, Percent_Identity=32.972972972973, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790453, Length=225, Percent_Identity=27.1111111111111, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 31422; Mature: 31290

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTTQSSQVSVSQPPVSKSMNKNKFPPKRANSSTSSPAKSTIKPAKPASRGPVNRAAA
CCCCCCCCCEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHH
VDSKVAPARNANGSLKKEPFKNPNRRAIAAAKPVQSQTIAPIIVLFNKPFDVLCQFTDEQ
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHEEEECCHH
GRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYWVQVEGVPT
HHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCEEEEEEEEECCCC
EDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQ
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCC
VRRMTAHIGFPTLRLIRYKIGQWSLDNLPSGEHKCIRLGDEV
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
TTLTTQSSQVSVSQPPVSKSMNKNKFPPKRANSSTSSPAKSTIKPAKPASRGPVNRAAA
CCCCCCCCEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHH
VDSKVAPARNANGSLKKEPFKNPNRRAIAAAKPVQSQTIAPIIVLFNKPFDVLCQFTDEQ
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHEEEECCHH
GRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYWVQVEGVPT
HHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCEEEEEEEEECCCC
EDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQ
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCC
VRRMTAHIGFPTLRLIRYKIGQWSLDNLPSGEHKCIRLGDEV
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]