Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 160875652

GI number: 160875652

Start: 3003780

End: 3004607

Strand: Direct

Name: hisH [H]

Synonym: Sbal195_2540

Alternate gene names: 160875652

Gene position: 3003780-3004607 (Clockwise)

Preceding gene: 160875651

Following gene: 160875653

Centisome position: 56.17

GC content: 49.64

Gene sequence:

>828_bases
ATGCAACAAGACACTCTATTAACAGCTGGCCAGCAATTAACAGCTAGCCCGCAATTAACAGCAAATGAATCATCAGATGC
TGAAACCGTCATCATAGACACGGGCTGCGCCAATTTAAGTTCAGTACGTTTCGCCTTCGAGCGTTTAGGCGCTAAGGTAT
TGGTCACAGACGATAAAGCCAAAATCAAAGCGGCAAAACGTGTGGTGCTGCCGGGTGTAGGCACAGCGGGTGCCGCCATG
GCGTCCTTACATGAAAAAGGCTTGGTCGAACTCATCCAAAGCCTGACTCAACCTGTGTTGGGCGTATGTCTTGGCATGCA
AATGTTGGCGGCAATTTCTAAGGAGCGCGGCGGAAAAGGTCAAGATTGCGAGTGCTTAGGCATAATCCCAACCGACATTT
GTGAACTGGATACGCAGTTACTTAAAGCTGAAAGCTTACCTTTGCCACACATGGGTTGGAACCAACTTAAGCTAACAACA
AACTCAGAAGGCACCAACAGCCCTAACGTTCACCCACTGTTTGCGGGTATTGAAGATGGCAGCTATGTGTATTTCGTCCA
CAGTTACCGCGCGCCGTTGAGCGAATTCACCTTAGCCGAATGTACTTATGGCGAAGGCTTTAGCGCCGCCATCGGCAAAG
GCAACTTTATGGGCGTGCAGTTCCACCCCGAAAAAAGTGCCGCCGTGGGCGCGCAAATTCTGCGTAACTTTTTACAGATG
GACGAGTCACAGATGAACAAAGCTCTGATGACCGAAGCTCTGATGAACAAAGCGCTGCAAAGCAACTCTGCTGATGGCCA
AGCGGGTGTAAATAAGGCTGACCAATGA

Upstream 100 bases:

>100_bases
GGCCGCGCGCTACGCCAAGCGGTTAAAGTTGAAGGCGATATTCTGCCATCGAGCAAAGGCGTACTTTAAGGCATCAGCAT
AAATCGTTTAAGAGGTTAGG

Downstream 100 bases:

>100_bases
ATAAATTACTCATTAAAAAACCAGTTATTAATAAATTACTTATAAACAAATCAATTAAGGATCTGAGCTTATGATTATTC
CTGCGATTGATTTAATTGAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQQDTLLTAGQQLTASPQLTANESSDAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKAKIKAAKRVVLPGVGTAGAAM
ASLHEKGLVELIQSLTQPVLGVCLGMQMLAAISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLKLTT
NSEGTNSPNVHPLFAGIEDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQILRNFLQM
DESQMNKALMTEALMNKALQSNSADGQAGVNKADQ

Sequences:

>Translated_275_residues
MQQDTLLTAGQQLTASPQLTANESSDAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKAKIKAAKRVVLPGVGTAGAAM
ASLHEKGLVELIQSLTQPVLGVCLGMQMLAAISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLKLTT
NSEGTNSPNVHPLFAGIEDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQILRNFLQM
DESQMNKALMTEALMNKALQSNSADGQAGVNKADQ
>Mature_275_residues
MQQDTLLTAGQQLTASPQLTANESSDAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKAKIKAAKRVVLPGVGTAGAAM
ASLHEKGLVELIQSLTQPVLGVCLGMQMLAAISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLKLTT
NSEGTNSPNVHPLFAGIEDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQILRNFLQM
DESQMNKALMTEALMNKALQSNSADGQAGVNKADQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=48.5849056603774, Blast_Score=201, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319725, Length=244, Percent_Identity=31.5573770491803, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 29223; Mature: 29223

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQDTLLTAGQQLTASPQLTANESSDAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKA
CCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCHH
KIKAAKRVVLPGVGTAGAAMASLHEKGLVELIQSLTQPVLGVCLGMQMLAAISKERGGKG
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLKLTTNSEGTNSPNVHPLFAGIEDG
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCC
SYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQILRNFLQM
CEEEEEECCCCCHHHHHHHHCCCCCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHC
DESQMNKALMTEALMNKALQSNSADGQAGVNKADQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQDTLLTAGQQLTASPQLTANESSDAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKA
CCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCHH
KIKAAKRVVLPGVGTAGAAMASLHEKGLVELIQSLTQPVLGVCLGMQMLAAISKERGGKG
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLKLTTNSEGTNSPNVHPLFAGIEDG
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCC
SYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQILRNFLQM
CEEEEEECCCCCHHHHHHHHCCCCCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHC
DESQMNKALMTEALMNKALQSNSADGQAGVNKADQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]