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The map label for this gene is ilvI [H]

Identifier: 160875602

GI number: 160875602

Start: 2943733

End: 2945451

Strand: Direct

Name: ilvI [H]

Synonym: Sbal195_2490

Alternate gene names: 160875602

Gene position: 2943733-2945451 (Clockwise)

Preceding gene: 160875601

Following gene: 160875603

Centisome position: 55.05

GC content: 48.87

Gene sequence:

>1719_bases
ATGGAGATGTTATCCGGCGCCAGCATGATAGTGCGTTCTTTGATCGATGAAGGCGTAAAGCACATTTTTGGCTATCCTGG
CGGTTCAGTTTTAGATATCTACGACGCCCTGCACGTTATCCCCGGTATTGAACACATACTCGTTCGCCACGAACAAGCCG
CCGTACACATGGCCGATGGCTACGCCCGTGCCACGGGTAAAGTGGGCGTGGTATTAGTCACCTCAGGCCCAGGCGCGACC
AACGCGATTACCGGTATTGCCACCGCTTACATGGATTCTATTCCCCTTGTGGTGTTATCGGGTCAAGTCCCGAGCAATCT
TATCGGCAACGATGCCTTCCAAGAATGCGACATGATAGGTATCTCTCGCCCTGTAGTGAAACACAGCTTTTTAGTTAAAG
ATCCGACTGAAATTCCTGAAATTATTAAAAAAGCTTTTTACATTGCCTCTACTGGCCGTCCCGGTCCTGTGGTTGTCGAT
CTGCCAAAAGATTGTTTAAACCCCGCCCTGCTGCACGAATATTGCTACCCAGAAAGCGTCAAAATGCGCTCGTATAATCC
AACGACATCAGGCCATAAAGGTCAGATCCGCCGTGGTCTTCAAGCTCTATTAGCCGCAAAAAAACCGGTACTTTATGTCG
GTGGCGGCGCAATTATCTCAGGTTGTGATAAGCAAATTCTGCAATTAGCTGAGAGATTAAATATTCCCGTCGTCAGCACG
CTGATGGGGCTTGGCGCCTTTCCAAGCACTCACAAAAACTGTTTAGGCATGCTCGGGATGCACGGTCGCTACGAAGCCAA
CATGACAATGCATAACTGCGATTTGATCTTCGGTATTGGTGTACGCTTCGACGATAGAACCACCAATAATGTGGAAAAAT
ACTGTCCGAACGCGACCATTTTGCACATAGATATCGATCCCTCTTCTATTTCGAAAACTATCCACGTGGATATTCCTATC
GTGGGTTCGGCCGACAGTATTTTAGACAGCATGTTGGCACTGTTAGATGAATCGAACGAACACAACGACAACGATGCCAT
TGCCACTTGGTGGAATGACATTGAACAATGGCGCAGCCGCGATTGTCTGGCCTATGACAAAAACAGTGGCCGCATCAAAC
CACAGCAAGTGATTGAAACCTTGTATAAGCTGACCAATGGCGATGCCTATGTCGCATCAGATGTTGGCCAGCACCAAATG
TTTGCCGCCTTGTACTATCCGTTCGATAAGCCGCGCCGCTGGATCAACTCAGGTGGCCTAGGGACAATGGGATTCGGCCT
GCCCGCCGCGATGGGCGTAAAAATGGCCATGCCTGATGCTACTGTTGTGTGTGTCACGGGTGATGGTTCCATCCAGATGA
ACATTCAAGAGCTTTCGACCGCGCTGCAATATGATACGCCGGTTAAAATCATCAACTTAAACAATCGTTTCCTTGGCATG
GTAAAACAATGGCAGGACATGATTTACTCTGGCCGTCACTCACATTCCTATATGGATTCAGTGCCTAACTTCGCCAAAAT
TGCCGAGGCCTATGGTCATGTTGGCATGACGATCAGCGATCCCGCCGAACTTGAGTCAAAAATGGCTGAAGCGCTGGCGA
TGAAAGACAGGCTGGTCTTTATCGATATCATGGTGGATGAAACTGAGCACGTGTACCCCATGTTGATCCGCGGTGGTGCG
ATGAATGAAATGTGGCTGACCAAGACGGAGAAATGCTAA

Upstream 100 bases:

>100_bases
CGGAGTCTGAATTACCATTCAGGCTTAATTTTTAGAGCGATTACCGTCGCTGTAACAAGATACTGTTTACCGTAACGGTA
CTATGCAATAGGAGTTCCAC

Downstream 100 bases:

>100_bases
TGCGTCGAATTATATCTGTACTATTAGAAAACCAATCCGGCGCCCTGTCTCGTGTGGTCGGTTTGTTTTCACAACGCGGT
TATAACATTGAAAGTTTAAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHEYCYPESVKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
LMGLGAFPSTHKNCLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATILHIDIDPSSISKTIHVDIPI
VGSADSILDSMLALLDESNEHNDNDAIATWWNDIEQWRSRDCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLTKTEKC

Sequences:

>Translated_572_residues
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHEYCYPESVKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
LMGLGAFPSTHKNCLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATILHIDIDPSSISKTIHVDIPI
VGSADSILDSMLALLDESNEHNDNDAIATWWNDIEQWRSRDCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLTKTEKC
>Mature_572_residues
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHEYCYPESVKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
LMGLGAFPSTHKNCLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATILHIDIDPSSISKTIHVDIPI
VGSADSILDSMLALLDESNEHNDNDAIATWWNDIEQWRSRDCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLTKTEKC

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=592, Percent_Identity=27.027027027027, Blast_Score=190, Evalue=4e-48,
Organism=Homo sapiens, GI21361361, Length=509, Percent_Identity=24.3614931237721, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=75.043630017452, Blast_Score=929, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=39.8576512455516, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=33.6466165413534, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=29.009009009009, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=24.1071428571429, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=582, Percent_Identity=25.4295532646048, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=591, Percent_Identity=25.7191201353638, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=597, Percent_Identity=23.785594639866, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=41.2371134020619, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=24.2616033755274, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=522, Percent_Identity=22.7969348659004, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=327, Percent_Identity=25.0764525993884, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=497, Percent_Identity=22.3340040241449, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922626, Length=594, Percent_Identity=22.5589225589226, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62639; Mature: 62639

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVDLPKDCLNPALLHEYCYPESV
CCCCHHHHEEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCHHHHHHHCCCCCE
KMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHCHHHHHHHHHHCCCCHHHH
LMGLGAFPSTHKNCLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATI
HHHCCCCCCCHHHHHHHCCCCCCEECCEEEEECEEEEEECEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTIHVDIPIVGSADSILDSMLALLDESNEHNDNDAIATWWNDIEQWRSR
EEEECCHHHCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
DCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQMFAALYYPFDKPRRWINSGGL
CCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHHH
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVF
HHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEE
IDIMVDETEHVYPMLIRGGAMNEMWLTKTEKC
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVDLPKDCLNPALLHEYCYPESV
CCCCHHHHEEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCHHHHHHHCCCCCE
KMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHCHHHHHHHHHHCCCCHHHH
LMGLGAFPSTHKNCLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATI
HHHCCCCCCCHHHHHHHCCCCCCEECCEEEEECEEEEEECEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTIHVDIPIVGSADSILDSMLALLDESNEHNDNDAIATWWNDIEQWRSR
EEEECCHHHCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
DCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQMFAALYYPFDKPRRWINSGGL
CCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHHH
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVF
HHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEE
IDIMVDETEHVYPMLIRGGAMNEMWLTKTEKC
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]