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The map label for this gene is dapE [H]

Identifier: 160875582

GI number: 160875582

Start: 2922422

End: 2923564

Strand: Direct

Name: dapE [H]

Synonym: Sbal195_2470

Alternate gene names: 160875582

Gene position: 2922422-2923564 (Clockwise)

Preceding gene: 160875581

Following gene: 160875583

Centisome position: 54.65

GC content: 51.88

Gene sequence:

>1143_bases
ATGATGCCAGCCGACGATTATCCTGTAACTGAACTGACAAAAGCGCTGATCGCCCGCCCTTCGGTGACGCCGCTTGATGA
AGGCTGTCAAACCTTAATGGCTGAAAGACTCAGCGCCATCGGCTTTAATATTGAACCTATGGTATTTGAAGACACCACCA
ATATGTGGGCTCGCCGTGGCAATGAAGGTCCGGTCTTTTGTTTTGCCGGCCACACAGATGTTGTGCCAACGGGTGATGTG
AGCCGCTGGCACACGCCGCCTTTTGTGCCCACCATCATTGATGGCTACTTATATGGCCGCGGCGCAGCGGACATGAAAGG
CTCACTGGCGGCGATGATCATTGCCACCGAACGCTTTGTGGCTAAACATCCCGATCATCATGGCTCTATCGCCTTTTTGA
TCACCAGTGATGAAGAAGGTCCGTTCATCAACGGCACGACGCGAGTCATTGATACCTTAGAAGCGCGCAACGAGAAGATC
ACCTGGGCATTAGTCGGCGAACCCTCATCAACCCTAAAGCTGGGTGATGTGGTCAAAAATGGTCGCCGCGGCAGTTTAAC
CGGCAACTTAACCGTGAAAGGCATTCAAGGCCACGTGGCCTATCCACACCTTGCCGATAATCCAATCCACAAAGCCGCGC
CTTTCTTAGCCGAGCTGAGTCAGATGCACTGGGATAATGGCAATGAGTTCTTTCCGCCCACCAGCTTCCAAATCGCCAAT
ATTAATGGCGGCACCGGCGCATCGAATGTGATCCCCGGCGCACTGGACGTGATGTTTAACTTCCGTTACTCCACCGAAGT
GAGCGCCGACATCTTGATTGAGCGGGTTGAAGCCTTATTGAAAGCCCATGAGCTAGATTACGATATCAGCTGGATTTTCA
ATGGCCTGCCATTTTTAACCGGCGATGGCCCACTGCTTGACGCGACCCGTATTGCTATTCGCCAAGTCACAGGCTATGAG
ACAGATCCACAAACCACAGGCGGCACATCGGACGGACGCTTTATCGCGCCAACGGGTGCCAAAGTGTTAGAGCTTGGCCC
AGTGAACGCTACCATTCATAAAGTGAATGAATGCGTTAAAGTGGATGACCTTGAGCAGCTAGCCCTGTGTTATGAAGTGA
TTTTGGAACAACTATTGTGCTAA

Upstream 100 bases:

>100_bases
TGCTGGCGCAACCGACACTGATCAAACGCCCCGTCTTGGTCGTCGGTGAACACGTGCTGATTGGCTTTGATGAAGCCGCC
TATAAAAGAGTATTTTCACT

Downstream 100 bases:

>100_bases
CACACCTTGAGATCCGCCCCGAAACCGCGAGGCTTTACGGCTTAAACTCCCCTGAATTGGTGGAATATTTAAGTCCGTAT
CACCTTGGGTATCATAGCTT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MMPADDYPVTELTKALIARPSVTPLDEGCQTLMAERLSAIGFNIEPMVFEDTTNMWARRGNEGPVFCFAGHTDVVPTGDV
SRWHTPPFVPTIIDGYLYGRGAADMKGSLAAMIIATERFVAKHPDHHGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWALVGEPSSTLKLGDVVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQMHWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALDVMFNFRYSTEVSADILIERVEALLKAHELDYDISWIFNGLPFLTGDGPLLDATRIAIRQVTGYE
TDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVKVDDLEQLALCYEVILEQLLC

Sequences:

>Translated_380_residues
MMPADDYPVTELTKALIARPSVTPLDEGCQTLMAERLSAIGFNIEPMVFEDTTNMWARRGNEGPVFCFAGHTDVVPTGDV
SRWHTPPFVPTIIDGYLYGRGAADMKGSLAAMIIATERFVAKHPDHHGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWALVGEPSSTLKLGDVVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQMHWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALDVMFNFRYSTEVSADILIERVEALLKAHELDYDISWIFNGLPFLTGDGPLLDATRIAIRQVTGYE
TDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVKVDDLEQLALCYEVILEQLLC
>Mature_380_residues
MMPADDYPVTELTKALIARPSVTPLDEGCQTLMAERLSAIGFNIEPMVFEDTTNMWARRGNEGPVFCFAGHTDVVPTGDV
SRWHTPPFVPTIIDGYLYGRGAADMKGSLAAMIIATERFVAKHPDHHGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWALVGEPSSTLKLGDVVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQMHWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALDVMFNFRYSTEVSADILIERVEALLKAHELDYDISWIFNGLPFLTGDGPLLDATRIAIRQVTGYE
TDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVKVDDLEQLALCYEVILEQLLC

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=373, Percent_Identity=62.1983914209115, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1790395, Length=275, Percent_Identity=29.8181818181818, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17551016, Length=227, Percent_Identity=29.9559471365639, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41335; Mature: 41335

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPADDYPVTELTKALIARPSVTPLDEGCQTLMAERLSAIGFNIEPMVFEDTTNMWARRG
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCC
NEGPVFCFAGHTDVVPTGDVSRWHTPPFVPTIIDGYLYGRGAADMKGSLAAMIIATERFV
CCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCEEEEEEEHHHHH
AKHPDHHGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWALVGEPSSTLKLGDVVKN
HCCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHC
GRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQMHWDNGNEFFPPTSFQIAN
CCCCCEEECEEEEEEECCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
INGGTGASNVIPGALDVMFNFRYSTEVSADILIERVEALLKAHELDYDISWIFNGLPFLT
CCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEC
GDGPLLDATRIAIRQVTGYETDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVK
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHC
VDDLEQLALCYEVILEQLLC
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMPADDYPVTELTKALIARPSVTPLDEGCQTLMAERLSAIGFNIEPMVFEDTTNMWARRG
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCC
NEGPVFCFAGHTDVVPTGDVSRWHTPPFVPTIIDGYLYGRGAADMKGSLAAMIIATERFV
CCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCEEEEEEEHHHHH
AKHPDHHGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWALVGEPSSTLKLGDVVKN
HCCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHC
GRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQMHWDNGNEFFPPTSFQIAN
CCCCCEEECEEEEEEECCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
INGGTGASNVIPGALDVMFNFRYSTEVSADILIERVEALLKAHELDYDISWIFNGLPFLT
CCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEC
GDGPLLDATRIAIRQVTGYETDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVK
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHC
VDDLEQLALCYEVILEQLLC
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]