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The map label for this gene is dacB [H]

Identifier: 160875500

GI number: 160875500

Start: 2818012

End: 2819520

Strand: Direct

Name: dacB [H]

Synonym: Sbal195_2388

Alternate gene names: 160875500

Gene position: 2818012-2819520 (Clockwise)

Preceding gene: 160875499

Following gene: 160875511

Centisome position: 52.7

GC content: 50.7

Gene sequence:

>1509_bases
ATGGAAACGTTACTGAAAACCACTCTGTATGCCAGCCTACTCTTAGCGGCGAGCCACAATGTCGCCCGCGCTGAGCAAAT
CGACGCAGGAGCGATAGAGACCCGAACCACTTCGCTTAGCCAAGCGGTCAGCAAAATCAGCCCTCGTCACTCGCAAATTG
CACTGCTGACAATGGACATGTCGAGCAATCAAGTGATCTTTAGCCAACAGGCTGAAACGCTGTTTATTCCCGCCAGCACG
CAAAAGCTACTCACTGCTGTGAGTGCCATGGCTCAGCTTGGACCTGATTTTCGTTATGTCACTGAATTATGGACGGACGC
ACCAATACGCAAGGGCCATATCGCCGGCAGCGCTTATCTGCGTTTTAGTGGCGATCCGACGTTCACTCAAGCCGATCTCA
AGGCCATTTTTGCCAGTCTCGTGAAACAAGGCATTAATCGCATTGATGGACATCTGTATTTGCTCGGCGATAAACAAGAG
CAAATGCAAGCCCCTGGTTGGGTTTGGGATGACTTAGGCATTTGTTTTGCAGCGCCTGTGTCGAGTTACATTATCAATCA
AAACTGCGTCTATGGTCAGTTCAACCCCAGCACGGCCAATAAGCCTTCACAGGTGAGCCTGCGCACGGGGAGTTATGGGG
TAAAAGTCAGCAGCGATGCGGTATTTGATCCCAAGGCGAGTCGCGAATTTTGCCAGCTCGATTTGGTGCGACTAGGCCAA
AACAACTATCATCTGCGTGGCTGTTATCCGGGCGGTGAAGCGATTGGACTGGCGATTGCGGTCAGCGATCCGTTCAAATT
TGCCCAAGATAACCTCACCTCGCTGCTCAAAGGTGAGATGTCACTGGCGGGTAAAGTTAAGCTCGGCACTAGCTTGCCCA
AAAAGGCGAAACTGATTGCCAGCCATAGCTCGGCGCCTCTGCCTGAATTGCTCGATACTATGCTGCTCAAGTCGGATAAT
TTGATTGCTGATAGCCTGTTTAAACAGCTTGGCAAAAGTTATTTCAATGCCCAAGGTAGCTTTACCAATGGCGCGGCAGC
CATGCAGCGGATTTTAACGGAGTTAGGCGTCGATCTGAGTAACGCTAACATTGTCGATGGTTCAGGGCTGTCACGCTATA
ACTTGCTCAATGCCCAGCAGTTAGCGGCGGTATTGGCACTCATCCATAAGGACAGCCGCTTTAGCCCGTTAATGACCAGT
CTGCCGCAAGCTGGAATAAGCGGCACCTTAAGGTATCGCATGGGCTACAACAAGCCGCCGCTGAAACAGCAAATTTTTGC
TAAAACAGGCTCGATGCAAGGCGTGTCTAACTTAGCGGGGTTTATCCGTTTGCCTGAGCACAGTGACACCCTGTTTGTGG
TGCTTGAGAATGGCATGAGTCCCGAGCTTAAACATCAGCATAAACCATCGTTTAATGCCGAGTTTCTCAAGCAATTGATT
TCAGTGCTGCAAGTGCCATCCCCCACCACAGCAGCGCTTAGTACCGCAGCTCACTCGATCCAAGATTAA

Upstream 100 bases:

>100_bases
ATGGAAAAGGATGATGGCAGCAGTGAGGATTAGCCCCACATTGCATCGAACTTAACCTGCAGGCCCATGATGTTATTAAT
AAAAATGGCCAATCGCTAAC

Downstream 100 bases:

>100_bases
ATACTGCCGAGTAGAAATAGCATGATATAAAACAAAAAAGCACTGAGTCATCAGTGCTTTTCTCGTTTTTATGTCGTTTT
TATTTAAACGAATCTATCAA

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
METLLKTTLYASLLLAASHNVARAEQIDAGAIETRTTSLSQAVSKISPRHSQIALLTMDMSSNQVIFSQQAETLFIPAST
QKLLTAVSAMAQLGPDFRYVTELWTDAPIRKGHIAGSAYLRFSGDPTFTQADLKAIFASLVKQGINRIDGHLYLLGDKQE
QMQAPGWVWDDLGICFAAPVSSYIINQNCVYGQFNPSTANKPSQVSLRTGSYGVKVSSDAVFDPKASREFCQLDLVRLGQ
NNYHLRGCYPGGEAIGLAIAVSDPFKFAQDNLTSLLKGEMSLAGKVKLGTSLPKKAKLIASHSSAPLPELLDTMLLKSDN
LIADSLFKQLGKSYFNAQGSFTNGAAAMQRILTELGVDLSNANIVDGSGLSRYNLLNAQQLAAVLALIHKDSRFSPLMTS
LPQAGISGTLRYRMGYNKPPLKQQIFAKTGSMQGVSNLAGFIRLPEHSDTLFVVLENGMSPELKHQHKPSFNAEFLKQLI
SVLQVPSPTTAALSTAAHSIQD

Sequences:

>Translated_502_residues
METLLKTTLYASLLLAASHNVARAEQIDAGAIETRTTSLSQAVSKISPRHSQIALLTMDMSSNQVIFSQQAETLFIPAST
QKLLTAVSAMAQLGPDFRYVTELWTDAPIRKGHIAGSAYLRFSGDPTFTQADLKAIFASLVKQGINRIDGHLYLLGDKQE
QMQAPGWVWDDLGICFAAPVSSYIINQNCVYGQFNPSTANKPSQVSLRTGSYGVKVSSDAVFDPKASREFCQLDLVRLGQ
NNYHLRGCYPGGEAIGLAIAVSDPFKFAQDNLTSLLKGEMSLAGKVKLGTSLPKKAKLIASHSSAPLPELLDTMLLKSDN
LIADSLFKQLGKSYFNAQGSFTNGAAAMQRILTELGVDLSNANIVDGSGLSRYNLLNAQQLAAVLALIHKDSRFSPLMTS
LPQAGISGTLRYRMGYNKPPLKQQIFAKTGSMQGVSNLAGFIRLPEHSDTLFVVLENGMSPELKHQHKPSFNAEFLKQLI
SVLQVPSPTTAALSTAAHSIQD
>Mature_502_residues
METLLKTTLYASLLLAASHNVARAEQIDAGAIETRTTSLSQAVSKISPRHSQIALLTMDMSSNQVIFSQQAETLFIPAST
QKLLTAVSAMAQLGPDFRYVTELWTDAPIRKGHIAGSAYLRFSGDPTFTQADLKAIFASLVKQGINRIDGHLYLLGDKQE
QMQAPGWVWDDLGICFAAPVSSYIINQNCVYGQFNPSTANKPSQVSLRTGSYGVKVSSDAVFDPKASREFCQLDLVRLGQ
NNYHLRGCYPGGEAIGLAIAVSDPFKFAQDNLTSLLKGEMSLAGKVKLGTSLPKKAKLIASHSSAPLPELLDTMLLKSDN
LIADSLFKQLGKSYFNAQGSFTNGAAAMQRILTELGVDLSNANIVDGSGLSRYNLLNAQQLAAVLALIHKDSRFSPLMTS
LPQAGISGTLRYRMGYNKPPLKQQIFAKTGSMQGVSNLAGFIRLPEHSDTLFVVLENGMSPELKHQHKPSFNAEFLKQLI
SVLQVPSPTTAALSTAAHSIQD

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=451, Percent_Identity=35.4767184035477, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 54283; Mature: 54283

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLLKTTLYASLLLAASHNVARAEQIDAGAIETRTTSLSQAVSKISPRHSQIALLTMDM
CCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEC
SSNQVIFSQQAETLFIPASTQKLLTAVSAMAQLGPDFRYVTELWTDAPIRKGHIAGSAYL
CCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEE
RFSGDPTFTQADLKAIFASLVKQGINRIDGHLYLLGDKQEQMQAPGWVWDDLGICFAAPV
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCEECCCCHHHHHHH
SSYIINQNCVYGQFNPSTANKPSQVSLRTGSYGVKVSSDAVFDPKASREFCQLDLVRLGQ
HHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHCCC
NNYHLRGCYPGGEAIGLAIAVSDPFKFAQDNLTSLLKGEMSLAGKVKLGTSLPKKAKLIA
CCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHCCHHHCEEEEECCCCCHHHHHEE
SHSSAPLPELLDTMLLKSDNLIADSLFKQLGKSYFNAQGSFTNGAAAMQRILTELGVDLS
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
NANIVDGSGLSRYNLLNAQQLAAVLALIHKDSRFSPLMTSLPQAGISGTLRYRMGYNKPP
CCEEECCCCCCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEECCCCCCC
LKQQIFAKTGSMQGVSNLAGFIRLPEHSDTLFVVLENGMSPELKHQHKPSFNAEFLKQLI
HHHHHHHHCCCCCCHHHHHHEEECCCCCCEEEEEEECCCCCCHHHCCCCCCCHHHHHHHH
SVLQVPSPTTAALSTAAHSIQD
HHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
METLLKTTLYASLLLAASHNVARAEQIDAGAIETRTTSLSQAVSKISPRHSQIALLTMDM
CCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEC
SSNQVIFSQQAETLFIPASTQKLLTAVSAMAQLGPDFRYVTELWTDAPIRKGHIAGSAYL
CCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEE
RFSGDPTFTQADLKAIFASLVKQGINRIDGHLYLLGDKQEQMQAPGWVWDDLGICFAAPV
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCEECCCCHHHHHHH
SSYIINQNCVYGQFNPSTANKPSQVSLRTGSYGVKVSSDAVFDPKASREFCQLDLVRLGQ
HHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHCCC
NNYHLRGCYPGGEAIGLAIAVSDPFKFAQDNLTSLLKGEMSLAGKVKLGTSLPKKAKLIA
CCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHCCHHHCEEEEECCCCCHHHHHEE
SHSSAPLPELLDTMLLKSDNLIADSLFKQLGKSYFNAQGSFTNGAAAMQRILTELGVDLS
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
NANIVDGSGLSRYNLLNAQQLAAVLALIHKDSRFSPLMTSLPQAGISGTLRYRMGYNKPP
CCEEECCCCCCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEECCCCCCC
LKQQIFAKTGSMQGVSNLAGFIRLPEHSDTLFVVLENGMSPELKHQHKPSFNAEFLKQLI
HHHHHHHHCCCCCCHHHHHHEEECCCCCCEEEEEEECCCCCCHHHCCCCCCCHHHHHHHH
SVLQVPSPTTAALSTAAHSIQD
HHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]