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The map label for this gene is aglA [H]

Identifier: 160875199

GI number: 160875199

Start: 2482861

End: 2484483

Strand: Direct

Name: aglA [H]

Synonym: Sbal195_2086

Alternate gene names: 160875199

Gene position: 2482861-2484483 (Clockwise)

Preceding gene: 160875192

Following gene: 160875200

Centisome position: 46.43

GC content: 46.89

Gene sequence:

>1623_bases
ATGAGTGAGTTAACTTGGTGGCGTGGCGCGGTAATTTATCAAATTTATCCGCGTAGCCTTTTAGATACCAATGGTGATGG
CGTTGGTGATTTGCGTGGCATTATCGCTAAGCTGAACTACATTGCGAGCCTCAACGTCGATGCCATCTGGATTTCACCAT
TCTTTAAATCGCCCATGGCAGATTTTGGCTACGACATCAGTGACTACCGCGAAGTTGACCCTCTTTTTGGCACTATGCAT
GATTTCGATGAATTGATTGAAAAAGCTCACGGCTTAGGCATTAAGGTCATTATCGATCAAGTATTAAGTCATACTTCTGA
TCAGCATGCGTGGTTTTTTGAAAGCCGCGAGAGCCGTACAAATCCCAAAGCGGATTGGTACGTGTGGGCCGATCCTAAGG
AAGATGGCACTGCGCCTAACAACTGGCTCGCGATTTTTGGCGGTTGTGCTTGGGAATGGGAACCGCGCAGACAGCAATAC
TATTTACATAACTTCCTGCGTAGCCAGCCCGATATCAACTTCCATAACCCTGACGTGCGTCAAGCTGTGCTGGATAATGT
CGAGTTCTGGCTTAAAAAGGGCGTTGATGGATTCCGCTTAGATGCGATTACCTTCTGTTATCACGATGAACAGCTCAGGG
ACAATCCGGCAAAACCTAAGGATAAACGTCAGGGCCGAGGTTTCAGTGAAGATAACCCTTACGCCTATCAATATCATTAC
TACAATAATGACCGTCCGCAAACCATCGCGTTTATTGAAGATTTACGAAAGCTTATTAATCGTTATCCTGGTGCGGTGAC
CTTAGGTGAAGTGTCTGCCGAAGACTCTTTAGCTGTGATGGCGGCGTACACTAAGGGTGAAGATCGCCTGCACATGGCCT
ACAGTTTTGAGCTACTGACCGACGATTACAGCGCCGCTTATATTCGCCAAACCGTAGAGGCATTAGAAGCGAGCATTGGT
GATGGCTGGCCTTGTTGGGCTATCGGTAATCATGATGTGCAGCGTGTGGCGAGCCGTTGGGGGCAGGGCAAACAGAATTC
AGACATGGTCAAAATGCTCAATGCCATGCTAAGTTCACTGCGCGGTAGTGTGTGTAGTTATCAAGGTGAAGAACTCGGTT
TAACCGAAGCGCCGATTGAATACCATGAGTTGCAAGATCCCTTTGGCAAAACCTTCTGGCCTATGTTCAAAGGCCGTGAT
GGTTGCCGTACGCCAATGCCATGGCAGCAAGGCGCCGATTTTAGTGGTTTTAGTGAGGTATCGCCTTGGCTACCGATAGC
ACCTGCACATCAAGCGTTAGCGGTAGATGTACAAGAATCAGATGCTGATTCCATGCTTAATGGTTATCGACGTTTTCTTG
CTTGGCGTAAATTACACCCAGTGCTGATTGAAGGCGATATCCAGTTTGTGGATACCAACGAGCCCATCTTGGCCTTTATC
CGCACCTTAGGAGAGCTCAAAATATTCGTCGGATTCAACTTAGGCGATACCAATGTTGAGTTGGATTTATCACAGTTGGA
ATTGGGTGAAATATTAACGGGACATGGCCTGAAAACCGCACAAATAGAACACAATCAGCTGAAGTTTTCGGCATTTGCCA
GCTTTTATGCAGTTTTAAACTAA

Upstream 100 bases:

>100_bases
AATAAGCCGCGATACCTTTACTCTGCAGTAACAAAGCGATAACTGTTTGCGTGAGTGTGTTAGGGAAAGATCGCTGTAGA
ATAACGATAAAGGGGCAACA

Downstream 100 bases:

>100_bases
AAATGATTCATTTTTATTCGGCTGAGGTGTTATATACCCAAACAACTTGAAGATGCAGGATTCAGCGGGAATTTAACGCA
CTTTAGGCAAGGCGGTTATT

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIIAKLNYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMH
DFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFFESRESRTNPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQY
YLHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
YNNDRPQTIAFIEDLRKLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIG
DGWPCWAIGNHDVQRVASRWGQGKQNSDMVKMLNAMLSSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRD
GCRTPMPWQQGADFSGFSEVSPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLIEGDIQFVDTNEPILAFI
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKTAQIEHNQLKFSAFASFYAVLN

Sequences:

>Translated_540_residues
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIIAKLNYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMH
DFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFFESRESRTNPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQY
YLHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
YNNDRPQTIAFIEDLRKLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIG
DGWPCWAIGNHDVQRVASRWGQGKQNSDMVKMLNAMLSSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRD
GCRTPMPWQQGADFSGFSEVSPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLIEGDIQFVDTNEPILAFI
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKTAQIEHNQLKFSAFASFYAVLN
>Mature_539_residues
SELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIIAKLNYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMHD
FDELIEKAHGLGIKVIIDQVLSHTSDQHAWFFESRESRTNPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQYY
LHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHYY
NNDRPQTIAFIEDLRKLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIGD
GWPCWAIGNHDVQRVASRWGQGKQNSDMVKMLNAMLSSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDG
CRTPMPWQQGADFSGFSEVSPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLIEGDIQFVDTNEPILAFIR
TLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKTAQIEHNQLKFSAFASFYAVLN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=534, Percent_Identity=32.9588014981273, Blast_Score=278, Evalue=1e-74,
Organism=Escherichia coli, GI1790687, Length=511, Percent_Identity=35.2250489236791, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1786604, Length=495, Percent_Identity=23.4343434343434, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32565753, Length=514, Percent_Identity=23.7354085603113, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25147709, Length=549, Percent_Identity=22.5865209471767, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321731, Length=557, Percent_Identity=35.1885098743267, Blast_Score=289, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6319776, Length=557, Percent_Identity=35.7271095152603, Blast_Score=289, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6322245, Length=556, Percent_Identity=33.273381294964, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321726, Length=553, Percent_Identity=32.1880650994575, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6324416, Length=553, Percent_Identity=32.5497287522604, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6322241, Length=553, Percent_Identity=32.5497287522604, Blast_Score=266, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6322021, Length=553, Percent_Identity=32.5497287522604, Blast_Score=266, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24583747, Length=527, Percent_Identity=37.7609108159393, Blast_Score=324, Evalue=8e-89,
Organism=Drosophila melanogaster, GI24583749, Length=527, Percent_Identity=37.7609108159393, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24586587, Length=522, Percent_Identity=36.9731800766284, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI24586591, Length=520, Percent_Identity=35.9615384615385, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24586593, Length=500, Percent_Identity=36, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24583745, Length=529, Percent_Identity=36.1058601134215, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24586599, Length=522, Percent_Identity=35.0574712643678, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24586589, Length=534, Percent_Identity=34.8314606741573, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24586597, Length=557, Percent_Identity=34.6499102333932, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI221330053, Length=526, Percent_Identity=33.8403041825095, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI45549022, Length=560, Percent_Identity=34.1071428571429, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI281360393, Length=443, Percent_Identity=33.4085778781038, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61639; Mature: 61507

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIIAKLNYIASLNVDAIWISPFFKSPMA
CCCCCCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHEECCCCEEEECHHHCCCHH
DFGYDISDYREVDPLFGTMHDFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFFESRESRT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
NPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLRSQPDINFHNPDVR
CCCCCEEEECCCCCCCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHH
QAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNDRPQTIAFIEDLRKLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLT
ECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEEEEEEEC
DDYSAAYIRQTVEALEASIGDGWPCWAIGNHDVQRVASRWGQGKQNSDMVKMLNAMLSSL
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDGCRTPMPWQQGADFSGFSEV
CCHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLIEGDIQFVDTNEPILAFI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCHHHHHH
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKTAQIEHNQLKFSAFASFYAVLN
HHHCCEEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIIAKLNYIASLNVDAIWISPFFKSPMA
CCCCCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHEECCCCEEEECHHHCCCHH
DFGYDISDYREVDPLFGTMHDFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFFESRESRT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
NPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLRSQPDINFHNPDVR
CCCCCEEEECCCCCCCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHH
QAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNDRPQTIAFIEDLRKLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLT
ECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEEEEEEEC
DDYSAAYIRQTVEALEASIGDGWPCWAIGNHDVQRVASRWGQGKQNSDMVKMLNAMLSSL
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDGCRTPMPWQQGADFSGFSEV
CCHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLIEGDIQFVDTNEPILAFI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCHHHHHH
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKTAQIEHNQLKFSAFASFYAVLN
HHHCCEEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]