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The map label for this gene is cphE [H]

Identifier: 160875164

GI number: 160875164

Start: 2437345

End: 2438970

Strand: Direct

Name: cphE [H]

Synonym: Sbal195_2050

Alternate gene names: 160875164

Gene position: 2437345-2438970 (Clockwise)

Preceding gene: 160875159

Following gene: 160875167

Centisome position: 45.58

GC content: 45.57

Gene sequence:

>1626_bases
ATGAAAACCTGCAGCTCTCAAGCTCCCGCAAATTGCCAATCGGAAGTCAAATTCTCAGCCGATGCCAAACGCGAAACACG
TTATGTGTTCAACGTTTCGTGGCTTGAACGTGCACTTAAACACCCAAGATTGCAGTCGTTAAATGAGCAACAGAAAGTGA
TATTACGGCAAGCGGCTAAAGCACTTTCTCCTGTCAGTAACAGTCTTGAAAATAGCGATGTTGAAATTGGCCGTTCACTT
GGCCGTTTATATAGTGACAGCTTAACCTTGGCAAGCCTTGAACAAGCCATTGCAAAGATAGATAAGCAATTTCTGGCGCA
TTTGAGCGACCAACAATATTACACACTGCTGGATTTACTGGAAGAAAAGCAAATGCTTGCTGACGGCACGCTGTTACAAG
AACAAGTGTTACTTGATTCCACAACCAATCCCCATGCCGCGACGCTTTATCAATTATTTACGCAGCAAGCGTTGGCAAAG
CGCCAGCAAAGCAACCCCAATGCCAAACGTCCACTGATCGCCGTTGTGACCGCTTCTGCGCGCGATCCCTTTGAAGCGGC
CCATTACTATGTCAGCGTATTTGAGCAAATGGGGGCCGAGGTTATTTGGCTGCCTTTAGATGCAGCGTTGCAAGCAGGTA
TCGCAACCCGTCAGTGTGACCAACTCGAAATGTTTAGACAGCAAATACAAGGTAATACGGATAGGGCGAGGCTTTATCCA
AAGCAAGTTGAGCTACAAAAGGTTTTCTGTGCCAGCCCCATTAAACTCTATCAATTACTTAATCAAATCGACGGACTCTT
TTTAAATGGCGGCGATCAGAGTTTGACTTTAAAGGCTTGGACGACGCCGGATGAATCACCCAGTGAAGCATTAAGCATCA
TCAAGAAACGTCTTTCCAAACAAGAAATTGTCATCGGTGGCACCAGCGCTGGCACTGCTGTGATGGCTGATAAAAATATG
ATCACCGGCGGGACGAGTGAGGGTGCCCTGAGTTTTGGTGTTTTCTCGACCGAAGCGCCAAGTGAGCGTTGTGAAGTCGC
TAATTGTGATACCTCAGTTCCTGCAACGGCGGTCACTTATCGCGAGCAAGGTGGGCTTGGATTTTTCCCCTTTGGTATAC
CCGACACACACTTTTCACAACGAGACCGTTATTTAAGACTCTTGCTCCTTAGCGCCAAGAGCGATAGCAAAATGGCATTT
GGAGTCGATGAAAATACAGCATTATTGGTCGATTTATCACATCGTTCGCTCTCAGTGGTCGGTGAGCATGGAGTATGGGC
ATTAGCGCAAGTTGAGCGATTTAAACAAAGCCAAGCTAACTCTAAAATAAATGCGGTTTCGCACTATTTAACTCCGGGTT
CCTATGCCGAAATTGACGCCACTGGTGAGCATTTGACTCATATCCGCCTAGCTCAGACTGCCAAATTAACGCCAATAATG
AGTAAGCAGAGTGACTTTTTTACTTGGGTCATGAATGCGTGTCGGCAAGGTATATATTCAACATCACTGAGTGATAAGTA
CGAATTAACTATTACTCCAATGGCGGCATTAAGTTGCCAAGGCATTTTAAATCAAGAACGCGGATATCAGTCGATGGATT
TAACACTAAAAGCGTTAGATAAATGA

Upstream 100 bases:

>100_bases
GCTATTTATCGCCCCAAGCGTGCAGTCAGCGCCAGTGAAAACCCTGAGTTTATCGATACCCACTCATCAACCATTTGATC
TTATGCTGGTGGGCGGAGGG

Downstream 100 bases:

>100_bases
CAGTAAGTTGAATTAATTTTAGTTGGATAGCTATGATATCAATAGGATAGGCTAAATTGATTAAGGTTAAGTATATATTA
GGTAGATTTTAAAAGTTGAG

Product: cyanophycinase-like exopeptidase-like protein

Products: NA

Alternate protein names: Extracellular CGPase; CPHEpa; Extracellular cyanophycinase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKTCSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAKALSPVSNSLENSDVEIGRSL
GRLYSDSLTLASLEQAIAKIDKQFLAHLSDQQYYTLLDLLEEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAK
RQQSNPNAKRPLIAVVTASARDPFEAAHYYVSVFEQMGAEVIWLPLDAALQAGIATRQCDQLEMFRQQIQGNTDRARLYP
KQVELQKVFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALSIIKKRLSKQEIVIGGTSAGTAVMADKNM
ITGGTSEGALSFGVFSTEAPSERCEVANCDTSVPATAVTYREQGGLGFFPFGIPDTHFSQRDRYLRLLLLSAKSDSKMAF
GVDENTALLVDLSHRSLSVVGEHGVWALAQVERFKQSQANSKINAVSHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
SKQSDFFTWVMNACRQGIYSTSLSDKYELTITPMAALSCQGILNQERGYQSMDLTLKALDK

Sequences:

>Translated_541_residues
MKTCSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAKALSPVSNSLENSDVEIGRSL
GRLYSDSLTLASLEQAIAKIDKQFLAHLSDQQYYTLLDLLEEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAK
RQQSNPNAKRPLIAVVTASARDPFEAAHYYVSVFEQMGAEVIWLPLDAALQAGIATRQCDQLEMFRQQIQGNTDRARLYP
KQVELQKVFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALSIIKKRLSKQEIVIGGTSAGTAVMADKNM
ITGGTSEGALSFGVFSTEAPSERCEVANCDTSVPATAVTYREQGGLGFFPFGIPDTHFSQRDRYLRLLLLSAKSDSKMAF
GVDENTALLVDLSHRSLSVVGEHGVWALAQVERFKQSQANSKINAVSHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
SKQSDFFTWVMNACRQGIYSTSLSDKYELTITPMAALSCQGILNQERGYQSMDLTLKALDK
>Mature_541_residues
MKTCSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAKALSPVSNSLENSDVEIGRSL
GRLYSDSLTLASLEQAIAKIDKQFLAHLSDQQYYTLLDLLEEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAK
RQQSNPNAKRPLIAVVTASARDPFEAAHYYVSVFEQMGAEVIWLPLDAALQAGIATRQCDQLEMFRQQIQGNTDRARLYP
KQVELQKVFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALSIIKKRLSKQEIVIGGTSAGTAVMADKNM
ITGGTSEGALSFGVFSTEAPSERCEVANCDTSVPATAVTYREQGGLGFFPFGIPDTHFSQRDRYLRLLLLSAKSDSKMAF
GVDENTALLVDLSHRSLSVVGEHGVWALAQVERFKQSQANSKINAVSHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
SKQSDFFTWVMNACRQGIYSTSLSDKYELTITPMAALSCQGILNQERGYQSMDLTLKALDK

Specific function: Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water- insoluble reserve polymer) into aspartate-arginine dipeptides [H]

COG id: COG4242

COG function: function code QP; Cyanophycinase and related exopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005320
- InterPro:   IPR011811 [H]

Pfam domain/function: PF03575 Peptidase_S51 [H]

EC number: =3.4.15.6 [H]

Molecular weight: Translated: 59798; Mature: 59798

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTCSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAK
CCCCCCCCCCCCHHCCEECCCCCCCCEEEEEHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
ALSPVSNSLENSDVEIGRSLGRLYSDSLTLASLEQAIAKIDKQFLAHLSDQQYYTLLDLL
HHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAKRQQSNPNAKRPLIAVVTASA
HHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
RDPFEAAHYYVSVFEQMGAEVIWLPLDAALQAGIATRQCDQLEMFRQQIQGNTDRARLYP
CCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCH
KQVELQKVFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALSIIKKRLSK
HHHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCC
QEIVIGGTSAGTAVMADKNMITGGTSEGALSFGVFSTEAPSERCEVANCDTSVPATAVTY
CEEEEECCCCCCEEEECCCEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCEEEEE
REQGGLGFFPFGIPDTHFSQRDRYLRLLLLSAKSDSKMAFGVDENTALLVDLSHRSLSVV
ECCCCCEEECCCCCCCCHHHHHHEEEEEEEECCCCCCEEEECCCCCEEEEEECCCCEEEE
GEHGVWALAQVERFKQSQANSKINAVSHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCCCHHHEEEHHHHHHCCCHH
SKQSDFFTWVMNACRQGIYSTSLSDKYELTITPMAALSCQGILNQERGYQSMDLTLKALD
CCCCHHHHHHHHHHHHCHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHCEEHHCCC
K
C
>Mature Secondary Structure
MKTCSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAK
CCCCCCCCCCCCHHCCEECCCCCCCCEEEEEHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
ALSPVSNSLENSDVEIGRSLGRLYSDSLTLASLEQAIAKIDKQFLAHLSDQQYYTLLDLL
HHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAKRQQSNPNAKRPLIAVVTASA
HHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
RDPFEAAHYYVSVFEQMGAEVIWLPLDAALQAGIATRQCDQLEMFRQQIQGNTDRARLYP
CCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCH
KQVELQKVFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALSIIKKRLSK
HHHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCC
QEIVIGGTSAGTAVMADKNMITGGTSEGALSFGVFSTEAPSERCEVANCDTSVPATAVTY
CEEEEECCCCCCEEEECCCEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCEEEEE
REQGGLGFFPFGIPDTHFSQRDRYLRLLLLSAKSDSKMAFGVDENTALLVDLSHRSLSVV
ECCCCCEEECCCCCCCCHHHHHHEEEEEEEECCCCCCEEEECCCCCEEEEEECCCCEEEE
GEHGVWALAQVERFKQSQANSKINAVSHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCCCHHHEEEHHHHHHCCCHH
SKQSDFFTWVMNACRQGIYSTSLSDKYELTITPMAALSCQGILNQERGYQSMDLTLKALD
CCCCHHHHHHHHHHHHCHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHCEEHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11986309 [H]